LOCUS       sequence-399706-       19583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express 'Frost Lisbon' Lemon FLS2-2 gene under the UBQ10 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19583)
  AUTHORS   Trinh J, Li T, Franco JY, Toruno TY, Stevens DM, Thapa SP, Wong J, 
            Pineda R, Avila de Dios E, Kahn TL, Seymour DK, Ramadugu C, Coaker 
            GL
  TITLE     Variation in microbial feature perception in the Rutaceae family 
            with immune receptor conservation in citrus.
  JOURNAL   Plant Physiol. 2023 May 5:kiad263. doi: 10.1093/plphys/kiad263.
  PUBMED    37144828
REFERENCE   2  (bases 1 to 19583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 May 5:kiad263. doi: 10.1093/plphys/kiad263."
COMMENT     Sequence Label: pUBQ10:LLFLS2-2
FEATURES             Location/Qualifiers
     source          1..19583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(145..516)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            549..658
                     /note="incP origin of transfer"
     primer_bind     881..900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1134..1151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1339..1989)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2454..2478
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2602..2781
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2634..2817
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2838..3632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2892..2911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3502..3521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4859..4864,4865..4882,4883..4889)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4897..4913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4902..4924
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4921..4937
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4921..4937
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    5972..5996
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9715..9739)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9768..9857
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      9891..10143
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(10572..10824)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11292..12317)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(11292..12317)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(12327..12671)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(12363..12383)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(12813..12830)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12813..12829)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12822..12844)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12969..12988)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13179..13200
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    13447..13471
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(15067..16215)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(16514..17308)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  17500..18267
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      join(18960..19583,1..8)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tgagcgtcgg aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgatca ggatattctt gtttaagatg
     4981 ttgaactcta tggaggtttg tatgaactga tgatctagga ccggataagt tcccttcttc
     5041 atagcgaact tattcaaaga atgttttgtg tatcattctt gttacattgt tattaatgaa
     5101 aaaatattat tggtcattgg actgaacacg agtgttaaat atggaccagg ccccaaataa
     5161 gatccattga tatatgaatt aaataacaag aataaatcga gtcaccaaac cacttgcctt
     5221 ttttaacgag acttgttcac caacttgata caaaagtcat tatcctatgc aaatcaataa
     5281 tcatacaaaa atatccaata acactaaaaa attaaaagaa atggataatt tcacaatatg
     5341 ttatacgata aagaagttac ttttccaaga aattcactga ttttataagc ccacttgcat
     5401 tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa agcacgaaga attctagaaa
     5461 atacgaaata cgcttcaatg cagtgggacc cacggttcaa ttattgccaa ttttcagctc
     5521 caccgtatat ttaaaaaata aaacgataat gctaaaaaaa tataaatcgt aacgatcgtt
     5581 aaatctcaac ggctggatct tatgacgacc gttagaaatt gtggttgtcg acgagtcagt
     5641 aataaacggc gtcaaagtgg ttgcagccgg cacacacgag tcgtgtttat caactcaaag
     5701 cacaaatact tttcctcaac ctaaaaataa ggcaattagc caaaaacaac tttgcgtgta
     5761 aacaacgctc aatacacgtg tcattttatt attagctatt gcttcaccgc cttagctttc
     5821 tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct ataaaacaat acccaaagag
     5881 ctcttcttct tcacaattca gatttcaatt tctcaaaatc ttaaaaactt tctctcaatt
     5941 ctctctaccg tgatcaaggc tagagttatc aacaagtttg tacaaaaaag caggctccgc
     6001 ggccgccccc ttcaccatgg agtctcaaac agtcagctta agcttaatcg tagtcatatt
     6061 tttttctctc ttttccactg ttgcattatc aactgcagaa cgaagcttag aagaagtaga
     6121 aactgaagcc ttgagggcct ttaagaatgg catcaccagc gaccctttag gagcactcgc
     6181 cgattggaat ggcgcaaatc aaattcatca ctgcaactgg tctggaatca cctgtgaccc
     6241 ttcgtcaaac catgtcatag ccattaaact cgtcgacaag cagctccaag gccagatatc
     6301 tccattcctt cgcaaccttt cagccctcca ggttcttgat ttatctttga attcattctc
     6361 tgggagtatt ccggctcagc tggggcaatg ctcccagctt gctgaactaa ctctgtacca
     6421 taattctctt tcgggttcca ttccaccaga aataggatcc cttcagaatc tgcaagcaat
     6481 ggacttagga aataacttct tgaatggaag catacctgaa agcatttgca actgtacttc
     6541 cttgttagcg cttggtctca ttttcaacaa tcttactgga acaatcccaa aagacatagg
     6601 aaatttgaga agccttcaaa tgtttgtagc ataccacaac aagcttgtag gttcaattcc
     6661 agtttccatg ggtagattag aagccttgca agctttagat gtgagtcaga acatgttgtc
     6721 gggaacaata cctccagaaa taggaaacct gtcgaactta gaatatcttc agttatttga
     6781 aaattctatt ggaggaagaa tcccgtccca actaggcaat tttacaaagc ttctcgccct
     6841 tgaactgtac accaatcagc tcaccggaag cattccctct gagcttggaa atttggctag
     6901 cttacaatca atgcatctat acaaaaatag cttgaattcc acaatcccaa tttctttgtt
     6961 ccagctgaaa tctttaactc gtttaggcct ctccgagaat gagttgactg gaacattacc
     7021 cctcgaacta ggatctctaa ggtcattacg agtattgacc ctacattcca ataagttcac
     7081 tggggagatc ccttcttcat tgacaaacct gacaaattta acatatttgt caatgagctt
     7141 caatttctta acagggaagc ttccatcaaa cattggcttg cttcataacc tcgaaaatct
     7201 gaccatgaat aacaatcttc ttgagggaac cattccatct agtatcacga actgtaccca
     7261 tctcgcatcc ataggtttgg cattcaatag gattactggg agaattccca ctggtttagg
     7321 aaagatgcaa aaccttacat tcctatcctt gtcatcaaat ggtatgtccg gagaaatccc
     7381 cgatgacctt ttcaactgtt caaatcttga aattcttgat ctgagtgaaa acaatttcag
     7441 tggactgctg aaaccaggag tcggaaagct taataatctc aagattatga aaatccgaac
     7501 aaatttcttt gttgggccaa tcccaccaga gatcggcaat ctgagccaac tcatgacctt
     7561 gtctcttggt gaaaatagtt tcacaggtcc tattacacca gacttatcca agctttctct
     7621 ccttcaggga ctccttttga atcacaacca tctagaaggt gcaatacccg atagactgtt
     7681 tgaactgaag cagctaacct atcttgattt gcagcaaaac aagttgacag gtccaattcc
     7741 aaatgctatc tcaaatcttg ggtttctttc atacttgaac ctccaaggca acaatcttaa
     7801 cgggtcaata ccaataagca tggagcgtct tcatcgactt ttgaccctgg atctctctca
     7861 taaccattta acaggatccg ctccaggatc tatgattgca ggtatgggaa gtatacaaat
     7921 atatttgaac ttctcataca atttattgga gggaagtatt ccagatgaga ttggtaagct
     7981 ggaaatggca caagccattg acatttcaaa caataacctc tcagggaaaa ttcctaaaat
     8041 gcttcaaggc tgcagaaatc tgttctctct tgatctgtca ggaaataaac tttctggtgc
     8101 aatcggtgct gaagtttttg gtcgaatggg tatgcttatg agcttgaacc tttcaaaaaa
     8161 tggtttagat ggtgaaatcc ctgaagagtt ggcaaatttg aagcatctta gctcgctaga
     8221 cctttctcac aacaagttga cgggcatcat cccggaacgc ctgggcagtc tttccgcctt
     8281 gaaacaactg aacctttctt taaatcaact tgaaggccac gttcctgaaa ctggcatatt
     8341 cagaagcatt aacatgtcta gtttggtcgg aaaccaagct ctctgcggaa caaaaactct
     8401 cagatcatgc agctcatcaa ggaaaaactc acaccattta tccaagagga caaagttggt
     8461 acttcgctgc tcagttgctg tggttctagt tctagttcta gtgctactga tgttaacact
     8521 ccgtcgatat cgatatgcca aaaataggac atcagaaagg gttgaggccc aagatcaaga
     8581 accagagttc atttccgcga tgacactaaa aaggtttgat cgaacagaat tagaaaatgc
     8641 taccggcttc ttcagtgaaa acaacattat tggtaacagc agtttgagta ctgtttacag
     8701 aggtcgactg gaagatgggc agattgtagc agtgaaaaaa ctgaatttcc atcaattctc
     8761 tgcagagtct gacaagagct tctaccgcga ggccaaaacc ttatcgaagc tgaagcacag
     8821 gaatctggtt aaggtgctgg ggtatgcttg ggaaagtggg aaactcaagg cattggttct
     8881 tgaatacatg gaaaatggaa gcttggaaag cgttatacat ggacctgggg tggatcactc
     8941 aaggtggacg ctgcccaaaa gaatagatgt tcttatttcc gttgccagtg ggttggatta
     9001 tttgcattca ggttatgact tccccattgt tcactgtgac ttgaagcctt ccaatattct
     9061 cttagacaga gactttgagg ctcatgtgag cgattttggt acgtctcgaa tgttggatgt
     9121 tcatctacaa gatgtctcct cattatcatc agcttttcaa ggcactattg gctacttggc
     9181 accaggtaaa tctttcaagc tcatcagtgt ttccttatta caggtcatga tttctataga
     9241 agtcttgtac taaatcatat atgtgttctt tctcatcaga gtttgcgtac atgaggacag
     9301 tcgcgactaa agtagatgtg tttagctttg gtatagtggt cacggagttc ttgacaaaac
     9361 ggaggccaac aggactggat gcagagaatg ggttgtcgcc tatcagcttg cgtcaacttg
     9421 tggagaaagc tgtggcaaat ggaatcaacg gggttcgtca aatcacagac cctaggctgg
     9481 ttttgagtat ttatgaagag caggagcagt accaagtatt ggaagagctc tttaaactgg
     9541 ccttggcttg tacttcctca aatcctgagg atcgtcccaa tatgaacgag gtgctgtcta
     9601 cgcttccgaa gctacgagaa acaagatttg gaaatcgtga tgaacacttt ccttccggtg
     9661 gagaatcaaa cacaccagcg cctttaaaag gacagttcaa gggtgggcgc gccgacccag
     9721 ctttcttgta caaagtggtt gataacagcg ggttaattaa catcttttac ccatacgatg
     9781 ttcctgacta tgcgggctat ccctatgacg tcccggacta tgcaggatcc tatccatatg
     9841 acgttccaga ttacgctgct cagtgcagcg cttagagctc gaatttcccc gatcgttcaa
     9901 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     9961 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
    10021 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
    10081 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
    10141 atcgggaatt agcttcatca acgcaagaca tgcgcacgac cgtctgacag gagaggaatt
    10201 tccgacgagc acagaaagga cttgctcttg gacgtaggcc tatttctcag gcacatgtat
    10261 caagtgttcg gacgtgggtt ttcgatggtg tatcagccgc cgccaactgg gagatgagga
    10321 ggctttcttg gggggcagtc agcagttcat ttcacaagac agaggaactt gtaaggagat
    10381 gcactgattt atcttggcgc aaaccagcag gacgaattag tgggaatagc ccgcgaatat
    10441 ctaagttatg cctgtcggca tgagcagaaa cttccaattc gaaacagttt ggagaggttg
    10501 tttttgggca taccttttgt tagtcagcct ctcgattgct catcgtcatt acacagtacc
    10561 gaagtttgat cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc
    10621 gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa
    10681 aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt
    10741 caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact
    10801 ttattgccaa atgtttgaac gatctgcttc gacgcactcc ttctttactc caccatctcg
    10861 tccttattga aaacgtgggt agcaccaaaa cgaatcaagt cgctggaact gaagttacca
    10921 atcacgctgg atgatttgcc agttggatta atcttgcctt tccccgcatg aataatattg
    10981 atgaatgcat gcgtgagggg tatttcgatt ttggcaatag ctgcaattgc cgcgacatcc
    11041 tccaacgagc ataattcttc agaaaaatag cgatgttcca tgttgtcagg gcatgcatga
    11101 tgcacgttat gaggtgacgg tgctaggcag tattccctca aagtttcata gtcagtatca
    11161 tattcatcat tgcattcctg caagagagaa ttgagacgca atccacacgc tgcggcaacc
    11221 ttccggcgtt cgtggtctat ttgctcttgg acgttgcaaa cgtaagtgtt ggatcccggt
    11281 cggcatctac tctattcctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg
    11341 gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta
    11401 cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct
    11461 gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc
    11521 atatacgccc ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc
    11581 gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat
    11641 tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc
    11701 gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc
    11761 tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc
    11821 atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat
    11881 gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga
    11941 tcggccgcag cgatcgcatc catggcctcc gcgaccggct gcagaacagc gggcagttcg
    12001 gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg
    12061 ctctcgctga attccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag
    12121 tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca
    12181 tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc
    12241 atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg
    12301 agttcaggct ttttcatatc ggggtcgtcc tctccaaatg aaatgaactt ccttatatag
    12361 aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
    12421 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
    12481 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct
    12541 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga
    12601 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt
    12661 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga
    12721 cgagagtgtc gtgctccacc atgttgacgg atctctagga cgcgtcgacg cgtcgacgcg
    12781 tcgacgcgtc gacgcgtcct agaagctaat tcactggccg tcgttttaca acgtcgtgac
    12841 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
    12901 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
    12961 ggcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
    13021 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
    13081 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
    13141 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
    13201 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
    13261 ggaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg
    13321 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa
    13381 agaaaaacca ccccagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt
    13441 caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca
    13501 gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg
    13561 gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc
    13621 aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa
    13681 cgatgacaga gcgttgctgc ctgtgatcaa atatcatctc cctcgcagag atccgaatta
    13741 tcagccttct tattcatttc tcgcttaacc gtgacaggct gtcgatcttg agaactatgc
    13801 cgacataata ggaaatcgct ggataaagcc gctgaggaag ctgagtggcg ctatttcttt
    13861 agaagtgaac gttgacgata tcaactcccc tatccattgc tcaccgaatg gtacaggtcg
    13921 gggacccgaa gttccgactg tcggcctgat gcatccccgg ctgatcgacc ccagatctgg
    13981 ggctgagaaa gcccagtaag gaaacaactg taggttcgag tcgcgagatc ccccggaacc
    14041 aaaggaagta ggttaaaccc gctccgatca ggccgagcca cgccaggccg agaacattgg
    14101 ttcctgtagg catcgggatt ggcggatcaa acactaaagc tactggaacg agcagaagtc
    14161 ctccggccgc cagttgccag gcggtaaagg tgagcagagg cacgggaggt tgccacttgc
    14221 gggtcagcac ggttccgaac gccatggaaa ccgcccccgc caggcccgct gcgacgccga
    14281 caggatctag cgctgcgttt ggtgtcaaca ccaacagcgc cacgcccgca gttccgcaaa
    14341 tagcccccag gaccgccatc aatcgtatcg ggctacctag cagagcggca gagatgaaca
    14401 cgaccatcag cggctgcaca gcgcctaccg tcgccgcgac cccgcccggc aggcggtaga
    14461 ccgaaataaa caacaagctc cagaatagcg aaatattaag tgcgccgagg atgaagatgc
    14521 gcatccacca gattcccgtt ggaatctgtc ggacgatcat cacgagcaat aaacccgccg
    14581 gcaacgcccg cagcagcata ccggcgaccc ctcggcctcg ctgttcgggc tccacgaaaa
    14641 cgccggacag atgcgccttg tgagcgtcct tggggccgtc ctcctgtttg aagaccgaca
    14701 gcccaatgat ctcgccgtcg atgtaggcgc cgaatgccac ggcatctcgc aaccgttcag
    14761 cgaacgcctc catgggcttt ttctcctcgt gctcgtaaac ggacccgaac atctctggag
    14821 ctttcttcag ggccgacaat cggatctcgc ggaaatcctg cacgtcggcc gctccaagcc
    14881 gtcgaatctg agccttaatc acaattgtca attttaatcc tctgtttatc ggcagttcgt
    14941 agagcgcgcc gtgcgtcccg agcgatactg agcgaagcaa gtgcgtcgag cagtgcccgc
    15001 ttgttcctga aatgccagta aagcgctggc tgctgaaccc ccagccggaa ctgaccccac
    15061 aaggccctag cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc
    15121 gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg cgccacttct tcacgcgggt
    15181 ggaatccgat ccgcacatga ggcggaaggt ttccagcttg agcgggtacg gctcccggtg
    15241 cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt acttctccca
    15301 tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt cgatcaggac
    15361 ctggcaacgg gacgttttct tgccacggtc caggacgcgg aagcggtgca gcagcgacac
    15421 cgattccagg tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga
    15481 caggcattcc tcggccttcg tgtaataccg gccattgatc gaccagccca ggtcctggca
    15541 aagctcgtag aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg cgtactccaa
    15601 cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat
    15661 cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt
    15721 cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt
    15781 gtccggccac ggcgcaatat cgaacaagga aagctgcatt tccttgatct gctgcttcgt
    15841 gtgtttcagc aacgcggcct gcttggcctc gctgacctgt tttgccaggt cctcgccggc
    15901 ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa
    15961 acctgccgcc tcctgttcga gacgacgcga acgctccacg gcggccgatg gcgcgggcag
    16021 ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt gctggaccat
    16081 cgagccgacg gactggaagg tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt
    16141 ttcggcatcc tcggcggaaa accccgcgtc gatcagttct tgcctgtatg ccttccggtc
    16201 aaacgtccga ttcattcacc ctccttgcgg gattgccccg actcacgccg gggcaatgtg
    16261 cccttattcc tgatttgacc cgcctggtgc cttggtgtcc agataatcca ccttatcggc
    16321 aatgaagtcg gtcccgtaga ccgtctggcc gtccttctcg tacttggtat tccgaatctt
    16381 gccctgcacg aataccagcg accccttgcc caaatacttg ccgtgggcct cggcctgaga
    16441 gccaaaacac ttgatgcgga agaagtcggt gcgctcctgc ttgtcgccgg catcgttgcg
    16501 ccacatctag gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat
    16561 caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct
    16621 ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc
    16681 caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt
    16741 cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct
    16801 cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat
    16861 cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac
    16921 aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct
    16981 tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca
    17041 tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct
    17101 ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat
    17161 accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct
    17221 tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca
    17281 attccggtga tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt
    17341 aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt
    17401 ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa
    17461 catagtatcg acggagccga ttttgaaacc acaattatgg gtgatgctgc caacttactg
    17521 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
    17581 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg
    17641 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
    17701 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
    17761 agcccgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
    17821 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
    17881 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
    17941 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
    18001 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
    18061 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
    18121 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
    18181 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
    18241 cactatcaat aagttggagt cattacccaa ttatgataga atttacaagc tataaggtta
    18301 ttgtcctggg tttcaagcat tagtccatgc aagtttttat gctttgccca ttctatagat
    18361 atattgataa gcgcgctgcc tatgccttgc cccctgaaat ccttacatac ggcgatatct
    18421 tctatataaa agatatatta tcttatcagt attgtcaata tattcaaggc aatctgcctc
    18481 ctcatcctct tcatcctctt cgtcttggta gctttttaaa tatggcgctt catagagtaa
    18541 ttctgtaaag gtccaattct cgttttcata cctcggtata atcttaccta tcacctcaaa
    18601 tggttcgctg ggtttatcgc acccccgaac acgagcacgg cacccgcgac cactatgcca
    18661 agaatgccca aggtaaaaat tgccggcccc gccatgaagt ccgtgaatgc cccgacggcc
    18721 gaagtgaagg gcaggccgcc acccaggccg ccgccctcac tgcccggcac ctggtcgctg
    18781 aatgtcgatg ccagcacctg cggcacgtca atgcttccgg gcgtcgcgct cgggctgatc
    18841 gcccatcccg ttactgcccc gatcccggca atggcaagga ctgccagcgc tgccattttt
    18901 ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg gggatgggag gcccgcgtta
    18961 gcgggccggg agggttcgag aagggggggc accccccttc ggcgtgcgcg gtcacgcgca
    19021 cagggcgcag ccctggttaa aaacaaggtt tataaatatt ggtttaaaag caggttaaaa
    19081 gacaggttag cggtggccga aaaacgggcg gaaacccttg caaatgctgg attttctgcc
    19141 tgtggacagc ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac tctgcccctc
    19201 aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg
    19261 cagggcactt atccccaggc ttgtccacat catctgtggg aaactcgcgt aaaatcaggc
    19321 gttttcgccg atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc
    19381 ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca
    19441 tctgtcagtg agggccaagt tttccgcgag gtatccacaa cgccggcggc cgcggtgtct
    19501 cgcacacggc ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg ccgccagccc
    19561 agcggcgagg gcaaccagcc cgg
//
