LOCUS       sequence-399707-       19504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Arabidopsis thaliana FLS2 gene under UBQ10 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19504)
  AUTHORS   Trinh J, Li T, Franco JY, Toruno TY, Stevens DM, Thapa SP, Wong J, 
            Pineda R, Avila de Dios E, Kahn TL, Seymour DK, Ramadugu C, Coaker 
            GL
  TITLE     Variation in microbial feature perception in the Rutaceae family 
            with immune receptor conservation in citrus.
  JOURNAL   Plant Physiol. 2023 May 5:kiad263. doi: 10.1093/plphys/kiad263.
  PUBMED    37144828
REFERENCE   2  (bases 1 to 19504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 May 5:kiad263. doi: 10.1093/plphys/kiad263."
COMMENT     Sequence Label: pUBQ10:AtFLS2
FEATURES             Location/Qualifiers
     source          1..19504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(145..516)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            549..658
                     /note="incP origin of transfer"
     primer_bind     881..900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1134..1151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1339..1989)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2454..2478
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2602..2781
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2634..2817
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2838..3632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2892..2911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3502..3521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4859..4864,4865..4882,4883..4889)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4897..4913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4902..4924
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4921..4937
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4921..4937
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    5972..5996
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9636..9660)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9689..9778
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      9812..10064
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(10493..10745)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11213..12238)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(11213..12238)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(12248..12592)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(12284..12304)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(12734..12751)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12734..12750)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12743..12765)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12890..12909)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13100..13121
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    13368..13392
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(14988..16136)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(16435..17229)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  17421..18188
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      join(18881..19504,1..8)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tgagcgtcgg aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgatca ggatattctt gtttaagatg
     4981 ttgaactcta tggaggtttg tatgaactga tgatctagga ccggataagt tcccttcttc
     5041 atagcgaact tattcaaaga atgttttgtg tatcattctt gttacattgt tattaatgaa
     5101 aaaatattat tggtcattgg actgaacacg agtgttaaat atggaccagg ccccaaataa
     5161 gatccattga tatatgaatt aaataacaag aataaatcga gtcaccaaac cacttgcctt
     5221 ttttaacgag acttgttcac caacttgata caaaagtcat tatcctatgc aaatcaataa
     5281 tcatacaaaa atatccaata acactaaaaa attaaaagaa atggataatt tcacaatatg
     5341 ttatacgata aagaagttac ttttccaaga aattcactga ttttataagc ccacttgcat
     5401 tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa agcacgaaga attctagaaa
     5461 atacgaaata cgcttcaatg cagtgggacc cacggttcaa ttattgccaa ttttcagctc
     5521 caccgtatat ttaaaaaata aaacgataat gctaaaaaaa tataaatcgt aacgatcgtt
     5581 aaatctcaac ggctggatct tatgacgacc gttagaaatt gtggttgtcg acgagtcagt
     5641 aataaacggc gtcaaagtgg ttgcagccgg cacacacgag tcgtgtttat caactcaaag
     5701 cacaaatact tttcctcaac ctaaaaataa ggcaattagc caaaaacaac tttgcgtgta
     5761 aacaacgctc aatacacgtg tcattttatt attagctatt gcttcaccgc cttagctttc
     5821 tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct ataaaacaat acccaaagag
     5881 ctcttcttct tcacaattca gatttcaatt tctcaaaatc ttaaaaactt tctctcaatt
     5941 ctctctaccg tgatcaaggc tagagttatc aacaagtttg tacaaaaaag caggctccgc
     6001 ggccgccccc ttcaccatga agttactctc aaagaccttt ttgatattaa ctctcacctt
     6061 cttcttcttt ggcattgcac tagcgaaaca gagctttgaa ccagagatcg aagctttgaa
     6121 atccttcaag aatggtattt ccaacgaccc tttaggagta ttatcagatt ggaccatcat
     6181 cggttcgtta cgacactgta attggaccgg aatcacctgc gatagtaccg gacatgtagt
     6241 ctcggtttcc ttgctggaga agcaacttga aggtgttctg tctccagcca tagcgaatct
     6301 cacctatctc caggttcttg atctcacttc aaatagtttt accggcaaaa taccggctga
     6361 aataggaaag ttaaccgagc ttaaccagct tattctgtac ctaaactatt tctctggttc
     6421 gattccttct ggaatctggg agcttaagaa tattttctat cttgatctta gaaataattt
     6481 gttgtccggt gatgttcctg aggaaatctg caaaaccagt tctttggtat tgattgggtt
     6541 tgattacaac aacttaaccg ggaaaatacc agaatgctta ggagatttgg ttcatctcca
     6601 aatgtttgta gcagctggta accatttaac tggttcgatt ccggtatcaa ttggtactct
     6661 ggctaattta acggatttag acctgagtgg taaccagtta accggaaaaa taccgagaga
     6721 ttttggaaat ctcttgaact tacagtctct cgttttaact gaaaacttgt tggaaggaga
     6781 tataccagct gagatcggaa actgctcgag cttggtccaa cttgagcttt acgataacca
     6841 gttaaccggg aaaataccag ctgaattagg gaatttggtt cagctgcaag cactccggat
     6901 atacaagaac aaacttactt cttcaattcc atcttcattg ttccggttaa ctcagttaac
     6961 ccatttgggg ttatcagaaa accatttggt tggaccgata tcagaagaaa tcggttttct
     7021 tgagtcactt gaagtcctca cacttcattc caacaacttc acaggagagt ttccacagtc
     7081 catcacaaac ttgaggaact tgacagtcct aacggtgggg ttcaataata tttccggtga
     7141 gctcccggcg gatctagggc ttcttacaaa ccttcggaac ctttcagcgc acgacaatct
     7201 tcttaccgga ccaatacctt ccagcataag taactgcacc ggtcttaaac tcctggacct
     7261 gtctcacaac caaatgactg gcgagatccc gcggggtttc ggaaggatga atcttacgtt
     7321 catttctatt gggaggaatc atttcaccgg tgaaattcca gatgatatct tcaactgttc
     7381 aaacttggaa actcttagtg tggcagataa caacttaaca ggaactctca agccattaat
     7441 tgggaagctt caaaaactca ggattttgca agtttcatat aactctctca ctggaccgat
     7501 tcctcgagaa atcgggaatc tgaaagattt gaatatcttg taccttcact ctaatggttt
     7561 cacagggaga atcccgagag agatgtcgaa tctcactctc ctccaggggc taaggatgta
     7621 ttcaaatgat cttgaaggtc caattcctga agaaatgttt gatatgaagc tactctcagt
     7681 tcttgatctt tccaacaaca aattctcagg tcaaattcct gccttgttct ccaagcttga
     7741 atcgcttacc tacttgagtc ttcaaggaaa caaattcaac gggtctatcc ctgcaagcct
     7801 taagtcgctt tcgcttctca acacattcga tatctccgac aatcttctca ctggaaccat
     7861 ccctggagag ctgttagctt ctttgaaaaa catgcagctt tacctcaact tctcaaacaa
     7921 cttgttgact ggaaccatcc caaaggagct tggaaagctt gaaatggttc aagaaatcga
     7981 cctttcaaac aatctctttt ctgggtctat tccaagatct ttacaggcct gcaaaaatgt
     8041 gttcacactg gatttttcgc agaacaatct ctcgggtcat ataccagatg aagtcttcca
     8101 aggcatggat atgatcataa gcttgaacct ttcaaggaac agtttctctg gagaaatccc
     8161 tcagagcttc gggaacatga cgcatttggt ctccttggat ctctctagta acaatctcac
     8221 tggtgaaatt ccagagagtc tcgccaatct ttcgactctg aaacatctca aactagcttc
     8281 aaacaacctc aaaggccatg ttcctgaatc cggggtgttc aaaaacatca acgcctctga
     8341 tctaatggga aacacagatc tctgtggtag caagaagcct ctcaagccat gtacgatcaa
     8401 gcagaagtcg agccacttct cgaagagaac cagagtcatc ctgattattc ttggatcagc
     8461 cgcggctctt cttcttgtcc tgcttcttgt tctgattcta acctgttgca agaaaaaaga
     8521 aaaaaagatt gaaaattcat cagagtcctc attaccggat ttggattcag ctctgaaact
     8581 gaagagattt gaaccaaaag agttggagca agcaacagat tcattcaaca gtgccaacat
     8641 cattggctca agcagcttaa gcacagtgta caaaggtcag ctagaagatg ggacagtgat
     8701 tgcagtaaaa gtattgaatc taaaggaatt ctctgcagaa tcagacaagt ggttctacac
     8761 agaagctaaa acattgagcc aactaaaaca tcgaaacctg gtgaagatct tagggtttgc
     8821 gtgggaaagc ggcaaaacga aagctttagt gcttccattt atggagaatg gaaacttgga
     8881 ggacaccatt cacggctctg cagcaccgat tgggtcgctt ttagaaaaaa tcgatctttg
     8941 tgttcatatc gcaagcggaa tcgattatct tcattctgga tatggttttc ccatcgttca
     9001 ttgtgatctg aagccagcta atatactcct tgacagtgac cgcgttgctc acgtaagcga
     9061 ttttggaact gctcggattc taggtttccg cgaagatgga agcaccacag cttcaacatc
     9121 agccttcgag ggtacaattg gatacttagc tccaggtaaa ttactcacaa cacttaattt
     9181 tcaaaaccct aattatgcca tcgacttact tgaaatacac tcttattttt catgaacaga
     9241 gtttgcttat atgaggaaag tgacaacaaa agccgatgta ttcagcttcg ggatcataat
     9301 gatggagctg atgacgaaac agagaccaac ttcgttgaat gatgaagatt cacaagacat
     9361 gactttgcgc caattggtgg agaaatcgat tggaaatgga agaaaaggga tggttagggt
     9421 tcttgatatg gaactcgggg actctattgt ttctctgaaa caggaagagg ctattgaaga
     9481 ctttctgaag ctttgtttgt tctgtacaag ctctagacct gaagatcgac ctgatatgaa
     9541 cgagattctt acacatctga tgaaacttag aggcaaagcg aattcatttc gagaagatcg
     9601 taacgaggat cgagaagtta agggtgggcg cgccgaccca gctttcttgt acaaagtggt
     9661 tgataacagc gggttaatta acatctttta cccatacgat gttcctgact atgcgggcta
     9721 tccctatgac gtcccggact atgcaggatc ctatccatat gacgttccag attacgctgc
     9781 tcagtgcagc gcttagagct cgaatttccc cgatcgttca aacatttggc aataaagttt
     9841 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
     9901 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     9961 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
    10021 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tagcttcatc
    10081 aacgcaagac atgcgcacga ccgtctgaca ggagaggaat ttccgacgag cacagaaagg
    10141 acttgctctt ggacgtaggc ctatttctca ggcacatgta tcaagtgttc ggacgtgggt
    10201 tttcgatggt gtatcagccg ccgccaactg ggagatgagg aggctttctt ggggggcagt
    10261 cagcagttca tttcacaaga cagaggaact tgtaaggaga tgcactgatt tatcttggcg
    10321 caaaccagca ggacgaatta gtgggaatag cccgcgaata tctaagttat gcctgtcggc
    10381 atgagcagaa acttccaatt cgaaacagtt tggagaggtt gtttttgggc ataccttttg
    10441 ttagtcagcc tctcgattgc tcatcgtcat tacacagtac cgaagtttga tcgatctagt
    10501 aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct
    10561 atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac
    10621 gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata
    10681 atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa
    10741 cgatctgctt cgacgcactc cttctttact ccaccatctc gtccttattg aaaacgtggg
    10801 tagcaccaaa acgaatcaag tcgctggaac tgaagttacc aatcacgctg gatgatttgc
    10861 cagttggatt aatcttgcct ttccccgcat gaataatatt gatgaatgca tgcgtgaggg
    10921 gtatttcgat tttggcaata gctgcaattg ccgcgacatc ctccaacgag cataattctt
    10981 cagaaaaata gcgatgttcc atgttgtcag ggcatgcatg atgcacgtta tgaggtgacg
    11041 gtgctaggca gtattccctc aaagtttcat agtcagtatc atattcatca ttgcattcct
    11101 gcaagagaga attgagacgc aatccacacg ctgcggcaac cttccggcgt tcgtggtcta
    11161 tttgctcttg gacgttgcaa acgtaagtgt tggatcccgg tcggcatcta ctctattcct
    11221 ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc
    11281 catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc
    11341 cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt
    11401 caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc cggagccgcg
    11461 gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag
    11521 ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg
    11581 cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc
    11641 cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct
    11701 catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat
    11761 acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc
    11821 cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat
    11881 ccatggcctc cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc aggtcttgca
    11941 acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgctg aattccccaa
    12001 tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat
    12061 ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat
    12121 cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt
    12181 cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat
    12241 cggggtcgtc ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg
    12301 atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt
    12361 tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc
    12421 tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg
    12481 gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg
    12541 gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct
    12601 ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac
    12661 catgttgacg gatctctagg acgcgtcgac gcgtcgacgc gtcgacgcgt cgacgcgtcc
    12721 tagaagctaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
    12781 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
    12841 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgcccgc tcctttcgct
    12901 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
    12961 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
    13021 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
    13081 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
    13141 tcgggctatt cttttgattt ataagggatt ttgccgattt cggaaccacc atcaaacagg
    13201 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
    13261 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accccagtac
    13321 attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa
    13381 tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca
    13441 ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg
    13501 cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg
    13561 aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg
    13621 cctgtgatca aatatcatct ccctcgcaga gatccgaatt atcagccttc ttattcattt
    13681 ctcgcttaac cgtgacaggc tgtcgatctt gagaactatg ccgacataat aggaaatcgc
    13741 tggataaagc cgctgaggaa gctgagtggc gctatttctt tagaagtgaa cgttgacgat
    13801 atcaactccc ctatccattg ctcaccgaat ggtacaggtc ggggacccga agttccgact
    13861 gtcggcctga tgcatccccg gctgatcgac cccagatctg gggctgagaa agcccagtaa
    13921 ggaaacaact gtaggttcga gtcgcgagat cccccggaac caaaggaagt aggttaaacc
    13981 cgctccgatc aggccgagcc acgccaggcc gagaacattg gttcctgtag gcatcgggat
    14041 tggcggatca aacactaaag ctactggaac gagcagaagt cctccggccg ccagttgcca
    14101 ggcggtaaag gtgagcagag gcacgggagg ttgccacttg cgggtcagca cggttccgaa
    14161 cgccatggaa accgcccccg ccaggcccgc tgcgacgccg acaggatcta gcgctgcgtt
    14221 tggtgtcaac accaacagcg ccacgcccgc agttccgcaa atagccccca ggaccgccat
    14281 caatcgtatc gggctaccta gcagagcggc agagatgaac acgaccatca gcggctgcac
    14341 agcgcctacc gtcgccgcga ccccgcccgg caggcggtag accgaaataa acaacaagct
    14401 ccagaatagc gaaatattaa gtgcgccgag gatgaagatg cgcatccacc agattcccgt
    14461 tggaatctgt cggacgatca tcacgagcaa taaacccgcc ggcaacgccc gcagcagcat
    14521 accggcgacc cctcggcctc gctgttcggg ctccacgaaa acgccggaca gatgcgcctt
    14581 gtgagcgtcc ttggggccgt cctcctgttt gaagaccgac agcccaatga tctcgccgtc
    14641 gatgtaggcg ccgaatgcca cggcatctcg caaccgttca gcgaacgcct ccatgggctt
    14701 tttctcctcg tgctcgtaaa cggacccgaa catctctgga gctttcttca gggccgacaa
    14761 tcggatctcg cggaaatcct gcacgtcggc cgctccaagc cgtcgaatct gagccttaat
    14821 cacaattgtc aattttaatc ctctgtttat cggcagttcg tagagcgcgc cgtgcgtccc
    14881 gagcgatact gagcgaagca agtgcgtcga gcagtgcccg cttgttcctg aaatgccagt
    14941 aaagcgctgg ctgctgaacc cccagccgga actgacccca caaggcccta gcgtttgcaa
    15001 tgcaccaggt catcattgac ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg
    15061 caggcttcgc cgacctgctc gcgccacttc ttcacgcggg tggaatccga tccgcacatg
    15121 aggcggaagg tttccagctt gagcgggtac ggctcccggt gcgagctgaa atagtcgaac
    15181 atccgtcggg ccgtcggcga cagcttgcgg tacttctccc atatgaattt cgtgtagtgg
    15241 tcgccagcaa acagcacgac gatttcctcg tcgatcagga cctggcaacg ggacgttttc
    15301 ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca ccgattccag gtgcccaacg
    15361 cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc
    15421 gtgtaatacc ggccattgat cgaccagccc aggtcctggc aaagctcgta gaacgtgaag
    15481 gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca acacctgctg ccacaccagt
    15541 tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg
    15601 tggaaaatga ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac
    15661 agggcagagc gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca cggcgcaata
    15721 tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc
    15781 tgcttggcct cgctgacctg ttttgccagg tcctcgccgg cggtttttcg cttcttggtc
    15841 gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc ctcctgttcg
    15901 agacgacgcg aacgctccac ggcggccgat ggcgcgggca gggcaggggg agccagttgc
    15961 acgctgtcgc gctcgatctt ggccgtagct tgctggacca tcgagccgac ggactggaag
    16021 gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa
    16081 aaccccgcgt cgatcagttc ttgcctgtat gccttccggt caaacgtccg attcattcac
    16141 cctccttgcg ggattgcccc gactcacgcc ggggcaatgt gcccttattc ctgatttgac
    16201 ccgcctggtg ccttggtgtc cagataatcc accttatcgg caatgaagtc ggtcccgtag
    16261 accgtctggc cgtccttctc gtacttggta ttccgaatct tgccctgcac gaataccagc
    16321 gaccccttgc ccaaatactt gccgtgggcc tcggcctgag agccaaaaca cttgatgcgg
    16381 aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc gccacatcta ggtactaaaa
    16441 caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa
    16501 gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca
    16561 gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact
    16621 tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag
    16681 ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg
    16741 agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc
    16801 caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga
    16861 gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc
    16921 ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc
    16981 atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat
    17041 catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt
    17101 taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc
    17161 cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat
    17221 tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct
    17281 aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag
    17341 aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg
    17401 attttgaaac cacaattatg ggtgatgctg ccaacttact gatttagtgt atgatggtgt
    17461 ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac
    17521 ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctatctctg ctctcactgc
    17581 cgtaaaacat ggcaactgca gttcacttac accgcttctc aacccggtac gcaccagaaa
    17641 atcattgata tggccatgaa tggcgttgga tgccgggcaa cagcccgcat tatgggcgtt
    17701 ggcctcaaca cgattttacg tcacttaaaa aactcaggcc gcagtcggta acctcgcgca
    17761 tacagccggg cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg ggctatgtcg
    17821 gggctaaatc gcgccagcgc tggctgtttt acgcgtatga cagtctccgg aagacggttg
    17881 ttgcgcacgt attcggtgaa cgcactatgg cgacgctggg gcgtcttatg agcctgctgt
    17941 caccctttga cgtggtgata tggatgacgg atggctggcc gctgtatgaa tcccgcctga
    18001 agggaaagct gcacgtaatc agcaagcgat atacgcagcg aattgagcgg cataacctga
    18061 atctgaggca gcacctggca cggctgggac ggaagtcgct gtcgttctca aaatcggtgg
    18121 agctgcatga caaagtcatc gggcattatc tgaacataaa acactatcaa taagttggag
    18181 tcattaccca attatgatag aatttacaag ctataaggtt attgtcctgg gtttcaagca
    18241 ttagtccatg caagttttta tgctttgccc attctataga tatattgata agcgcgctgc
    18301 ctatgccttg ccccctgaaa tccttacata cggcgatatc ttctatataa aagatatatt
    18361 atcttatcag tattgtcaat atattcaagg caatctgcct cctcatcctc ttcatcctct
    18421 tcgtcttggt agctttttaa atatggcgct tcatagagta attctgtaaa ggtccaattc
    18481 tcgttttcat acctcggtat aatcttacct atcacctcaa atggttcgct gggtttatcg
    18541 cacccccgaa cacgagcacg gcacccgcga ccactatgcc aagaatgccc aaggtaaaaa
    18601 ttgccggccc cgccatgaag tccgtgaatg ccccgacggc cgaagtgaag ggcaggccgc
    18661 cacccaggcc gccgccctca ctgcccggca cctggtcgct gaatgtcgat gccagcacct
    18721 gcggcacgtc aatgcttccg ggcgtcgcgc tcgggctgat cgcccatccc gttactgccc
    18781 cgatcccggc aatggcaagg actgccagcg ctgccatttt tggggtgagg ccgttcgcgg
    18841 ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg gagggttcga
    18901 gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta
    18961 aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg
    19021 aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat
    19081 gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc
    19141 cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg
    19201 cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag
    19261 gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg
    19321 ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag
    19381 ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc
    19441 gtttctggcg cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc
    19501 ccgg
//
