LOCUS       sequence-399708-        5916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pGL3 luciferase reporter with CHAF1B-SVA-del-VNTR sequence cloned 
            upstream of SV40 promoter to test effects of SVA-del-VNTR insertion 
            on luciferase expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Nadler MJS, Chang W, Ozkaynak E, Huo Y, Nong Y, Boillot M, Johnson 
            M, Moreno A, Matthew P Anderson
  TITLE     Hominoid SVA-lncRNA AK057321 targets human-specific SVA 
            retrotransposons in SCN8A and CDK5RAP2 to initiate neuronal 
            maturation.
  JOURNAL   Commun Biol. 2023 Mar 30;6(1):347. doi: 10.1038/s42003-023-04683-8.
  PUBMED    36997626
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-03-30"; volume: "6(1):347. doi"; pages: " 
            10.1038/s42003-023-04683-8"
COMMENT     Sequence Label: pGL3-luciferase-reporter-CHAF1B-SVA-del-VNTR
FEATURES             Location/Qualifiers
     source          1..5916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(73..92)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(752..1543,1544..1612))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1375..1394
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1613..1717)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1744..2199
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1831..1850)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2041..2062
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    2330..2378
                     /note="synthetic polyadenylation signal"
     misc_feature    2392..2483
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2432..2451
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        3444..3640
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3491..3626
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      3670..3679
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3676..5328
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3742..3760)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    5369..5490
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5380..5399)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5434..5453
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5738..5755)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(5909..5916,1..581))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
       61 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      121 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      181 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      241 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      301 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      361 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      421 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      481 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      541 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
      601 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
      661 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
      721 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
      781 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
      841 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
      901 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
      961 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     1021 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     1081 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     1141 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     1201 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     1261 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     1321 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     1381 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     1441 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     1501 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     1561 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     1621 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     1681 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     1741 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     1801 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     1861 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     1921 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     1981 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     2041 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     2101 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     2161 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg ccattcgcca ttcaggctgc
     2221 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag cccaagctac
     2281 catgataagt aagtaatatt aaggtacggg aggtacttgg agcggccgca ataaaatatc
     2341 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc
     2401 tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag
     2461 tgcaggtgcc agaacatttc tctatcgata ggtacccctc tccctctccc tccaccgtct
     2521 ccctctccct ctcccgctct ctccacagtc tccctctgat gccaagccga ggctggacgg
     2581 tactgccgcc atctcggctc actgcaacct ccctgcctga ttctcctgcc tcagcctgcc
     2641 gagtgcctgg gattgcaggc gcacgccgcc acgcctgtct ggttttcgta tttttttggt
     2701 ggagatgggg tttcgctgtg ttggccgggc tggtctccag ctcctaatcg cgagtgatct
     2761 gccagcctca gcctcccgag gtgccgggat tgcagacaga gtctcactca ctcagtgctc
     2821 aatgttgccc aggctggagg gcactggcgt ggtctccgct cgctacaacc tccacctccc
     2881 agccgcctgc cttggcctcc caaatgggag gtgtacccaa cagctcactg agaacgggcc
     2941 atgatgacga tggcggtttt gtcgaataga aaaaggggaa atgtggggaa aagatagaga
     3001 aatcagattg ttgctgtgtc tgtgtagaaa gaggtagaca taggagactc cattttgttc
     3061 tgtactaaga aaaattcttc tgccttggga tgctgctaat ctataacctt acccccaacc
     3121 ccctgctctc tgaaacatgt gctgtgtcaa ctcagggtta aatggattaa ggacggtaca
     3181 agatgtgctt tgttaaacag atgcttgaag gcagcatgct ccttcagagt catcaccact
     3241 ccctaatctc aagtacccag ggacacaaac actgcggaag gccactgggt cctctgccta
     3301 ggaaaaccag agacccttgt tcacttgttt atctgctgac cttccctcca ctattgtcct
     3361 atgaccctgc caaatccccc tctccgagaa acacccaaga atgatcaata aatactgcta
     3421 gcccgggctc gagatctgcg atctgcatct caattagtca gcaaccatag tcccgcccct
     3481 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatcgctg
     3541 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     3601 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggca ttccggtact
     3661 gttggtaaag ccaccatgga agacgccaaa aacataaaga aaggcccggc gccattctat
     3721 ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
     3781 gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag
     3841 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
     3901 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
     3961 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
     4021 aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa
     4081 attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa
     4141 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
     4201 tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat tgcactgatc
     4261 atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc
     4321 tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
     4381 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
     4441 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttctgagg
     4501 agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc
     4561 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggt
     4621 ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca tctgccaggt
     4681 atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
     4741 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
     4801 ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt gagaggtcct
     4861 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
     4921 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatcgtt
     4981 gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc tgaattggaa
     5041 tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct tcccgacgat
     5101 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
     5161 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
     5221 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
     5281 agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgtaatt ctagagtcgg
     5341 ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     5401 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     5461 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     5521 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     5581 aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     5641 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     5701 actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5761 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5821 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5881 accgtaaaaa ggccgcgttg ctggcgtttt tccata
//
