LOCUS       sequence-399748-        9061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector that expresses an shRNA that knocks down 
            SVA-lncRNA AK057321 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9061)
  AUTHORS   Nadler MJS, Chang W, Ozkaynak E, Huo Y, Nong Y, Boillot M, Johnson 
            M, Moreno A, Matthew P Anderson
  TITLE     Hominoid SVA-lncRNA AK057321 targets human-specific SVA 
            retrotransposons in SCN8A and CDK5RAP2 to initiate neuronal 
            maturation.
  JOURNAL   Commun Biol. 2023 Mar 30;6(1):347. doi: 10.1038/s42003-023-04683-8.
  PUBMED    36997626
REFERENCE   2  (bases 1 to 9061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-03-30"; volume: "6(1):347. doi"; pages: " 
            10.1038/s42003-023-04683-8"
COMMENT     Sequence Label: pLVX-SVA-lncRNA AK057321 shRNA 2S
FEATURES             Location/Qualifiers
     source          1..9061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     458..477
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(696..800)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    848..982
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(885..904)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     939..958
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1150..1783
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1830..1955
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2452..2685
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2870..2914
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3177..3292
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        3345..3593
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3345..3365
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3516..3535
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        3687..4898
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4735..4754
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4905..4961
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4974..5345
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     CDS             5355..5408
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      5430..5439
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5436..6146
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5581..5599)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5849..5868
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6179..6767
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6232..6252)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6650..6661)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6974..7607
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7735..7751)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7735..7751)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7748..7770)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7759..7775
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7783..7789,7790..7807,7808..7813))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7828..7849
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7966..7983)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8137..8725)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8217..8236)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8896..9061,1..626,627..695))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
       61 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
      121 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
      181 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
      241 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
      301 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
      361 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
      421 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
      481 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
      541 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
      601 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
      661 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
      721 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
      781 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
      841 ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
      901 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
      961 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     1021 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     1081 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     1141 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     1201 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     1261 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     1321 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     1381 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     1441 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     1501 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     1561 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     1621 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     1681 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     1741 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     1801 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     1861 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     1921 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     1981 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     2041 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     2101 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     2161 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     2221 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     2281 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     2341 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     2401 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     2461 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     2521 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     2581 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     2641 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     2701 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     2761 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     2821 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     2881 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     2941 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     3001 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     3061 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     3121 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta
     3181 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     3241 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     3301 attacaggga cagcagagat ccagtttatc gatctgggca ggaagagggc ctatttccca
     3361 tgattccttc atatttgcat atacgataca aggctgttag agagataatt agaattaatt
     3421 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
     3481 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
     3541 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagga tccgcatcaa
     3601 gatgcagtct cccttcaaga gagggagact gcatcttgat gttttttacg cgtgaattcg
     3661 aattaacccg tgtcggctcc agatctggcc tccgcgccgg gttttggcgc ctcccgcggg
     3721 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga
     3781 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc
     3841 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt
     3901 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg
     3961 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct
     4021 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca
     4081 cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt
     4141 gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg
     4201 ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg
     4261 gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg
     4321 gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat
     4381 gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt
     4441 ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct
     4501 ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag
     4561 ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt
     4621 cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat
     4681 aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc
     4741 tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac
     4801 cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt
     4861 ccgctaaatt ctggccgttt ttggcttttt tgttagactc tggcgccacg aatttctcgc
     4921 tactcaagca ggccggtgat gtcgaggaaa accctggtcc tatggtgtct agaatggcca
     4981 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
     5041 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc
     5101 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
     5161 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
     5221 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
     5281 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
     5341 aggacggttc aggggaggga agaggttccc tgctcacatg cggagacgta gaagagaatc
     5401 ccggacccat gacctctaga gaaccggtcg ccaccatggt gagcaagggc gaggaggata
     5461 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     5521 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     5581 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     5641 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     5701 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     5761 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     5821 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     5881 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     5941 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     6001 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     6061 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     6121 gcggcatgga cgagctgtac aagtagctcg agtctagagg gcccgacgcg tctggaacaa
     6181 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6241 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6301 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6361 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6421 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6481 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6541 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     6601 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6661 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6721 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc
     6781 tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     6841 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     6901 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     6961 gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     7021 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     7081 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     7141 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     7201 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     7261 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     7321 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     7381 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     7441 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7501 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7561 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     7621 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     7681 ttgacattgc tagcgtttac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     7741 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7801 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7861 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7921 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7981 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8041 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8101 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8161 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8221 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8281 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8341 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8401 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8461 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8521 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8581 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8641 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8701 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8761 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8821 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8881 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8941 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9001 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9061 t
//
