LOCUS       sequence-399750-        9060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector that expresses an shRNA that knocks down 
            SVA-lncRNA AK057321 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9060)
  AUTHORS   Nadler MJS, Chang W, Ozkaynak E, Huo Y, Nong Y, Boillot M, Johnson 
            M, Moreno A, Matthew P Anderson
  TITLE     Hominoid SVA-lncRNA AK057321 targets human-specific SVA 
            retrotransposons in SCN8A and CDK5RAP2 to initiate neuronal 
            maturation.
  JOURNAL   Commun Biol. 2023 Mar 30;6(1):347. doi: 10.1038/s42003-023-04683-8.
  PUBMED    36997626
REFERENCE   2  (bases 1 to 9060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-03-30"; volume: "6(1):347. doi"; pages: " 
            10.1038/s42003-023-04683-8"
COMMENT     Sequence Label: pLVX-SVA-lncRNA AK057321 shRNA 1S
FEATURES             Location/Qualifiers
     source          1..9060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     458..477
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(696..800)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    848..982
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(885..904)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     939..958
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1150..1783
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1830..1955
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2452..2685
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2870..2914
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3177..3292
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        3345..3593
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3345..3365
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3516..3535
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        3686..4897
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4734..4753
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4904..4960
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4973..5344
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     CDS             5354..5407
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      5429..5438
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5435..6145
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5580..5598)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5848..5867
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6178..6766
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6231..6251)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6649..6660)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6973..7606
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7734..7750)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7734..7750)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7747..7769)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7758..7774
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7782..7788,7789..7806,7807..7812))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7827..7848
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7965..7982)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8136..8724)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8216..8235)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8895..9060,1..626,627..695))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
       61 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
      121 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
      181 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
      241 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
      301 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
      361 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
      421 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
      481 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
      541 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
      601 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
      661 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
      721 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
      781 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
      841 ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
      901 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
      961 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     1021 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     1081 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     1141 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     1201 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     1261 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     1321 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     1381 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     1441 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     1501 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     1561 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     1621 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     1681 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     1741 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     1801 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     1861 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     1921 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     1981 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     2041 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     2101 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     2161 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     2221 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     2281 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     2341 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     2401 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     2461 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     2521 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     2581 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     2641 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     2701 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     2761 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     2821 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     2881 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     2941 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     3001 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     3061 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     3121 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta
     3181 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     3241 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     3301 attacaggga cagcagagat ccagtttatc gatctgggca ggaagagggc ctatttccca
     3361 tgattccttc atatttgcat atacgataca aggctgttag agagataatt agaattaatt
     3421 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
     3481 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
     3541 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagga tccgactata
     3601 tcatcatgta cattcaagag atgtacatga tgatatagtc ttttttacgc gtgaattcga
     3661 attaacccgt gtcggctcca gatctggcct ccgcgccggg ttttggcgcc tcccgcgggc
     3721 gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat
     3781 ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc
     3841 cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc
     3901 tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga
     3961 tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta
     4021 gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac
     4081 ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg
     4141 ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc
     4201 cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg
     4261 aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg
     4321 caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg
     4381 ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt
     4441 tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt
     4501 tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg
     4561 gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc
     4621 gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata
     4681 agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct
     4741 ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc
     4801 ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc
     4861 cgctaaattc tggccgtttt tggctttttt gttagactct ggcgccacga atttctcgct
     4921 actcaagcag gccggtgatg tcgaggaaaa ccctggtcct atggtgtcta gaatggccaa
     4981 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     5041 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     5101 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     5161 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     5221 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     5281 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     5341 ggacggttca ggggagggaa gaggttccct gctcacatgc ggagacgtag aagagaatcc
     5401 cggacccatg acctctagag aaccggtcgc caccatggtg agcaagggcg aggaggataa
     5461 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     5521 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     5581 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     5641 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     5701 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     5761 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     5821 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     5881 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     5941 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     6001 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     6061 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     6121 cggcatggac gagctgtaca agtagctcga gtctagaggg cccgacgcgt ctggaacaat
     6181 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     6241 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     6301 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     6361 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     6421 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     6481 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     6541 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     6601 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     6661 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     6721 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct
     6781 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     6841 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     6901 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6961 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     7021 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     7081 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     7141 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     7201 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     7261 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     7321 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     7381 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     7441 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7501 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7561 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     7621 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct
     7681 tgacattgct agcgtttacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     7741 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7801 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7861 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7921 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7981 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8041 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8101 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8161 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8221 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8281 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8341 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8401 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8461 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8521 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     8581 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8641 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8701 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8761 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8821 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8881 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8941 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9001 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
//
