LOCUS       sequence-399764-        4328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tardigrade (Hypsibius exemplaris) beta-tubulin Tub2C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Novotna Floriancicova K, Baltzis A, Smejkal J, Czernekova M, 
            Kaczmarek L, Maly J, Notredame C, Vinopal S
  TITLE     Phylogenetic and functional characterization of water bears 
            (Tardigrada) tubulins.
  JOURNAL   Sci Rep. 2023 Mar 30;13(1):5194. doi: 10.1038/s41598-023-31992-z.
  PUBMED    36997657
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-03-30"; volume: "13(1):5194. doi"; pages: " 
            10.1038/s41598-023-31992-z"
COMMENT     Sequence Label: pJet1.2-tHe-Tub2C
FEATURES             Location/Qualifiers
     source          1..4328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(2160..2166,2167..2184,2185..2190))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    2205..2226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2346..2363)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2517..3105)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2597..2616)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3276..4067,4068..4136))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3899..3918
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4137..4241)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4309..4327
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tcactagtca gtttccgcat cctcctcgtc aaaatctcct tcatcgtcgg
      421 cggttgcttc ttgatactgt tggtactccg agatgaggtc attcatattg ctctcagcct
      481 cagtgaactc catctcgtcc attccttctc cggtgtacca atgcaggaag gccttacgcc
      541 gaaacatggc ggtaaattgg tcggagatcc gtttgaacag ctcttggata gctgttgtgt
      601 taccaataaa ggtcgccgac atcttcagac cccgaggagg aatgtcacat accgcaatct
      661 tgacattgtt gggaatccat tcgacaaagt agctgctgtt tttgttctgc acgttcagca
      721 tattttcatc cacttccttc atactcatcc ggccgcgaaa gatggccgca acagtcaagt
      781 accgcccgtg gcggggatcg caggccgcca tcatattttt cgagtcaaac atctgggtgg
      841 tcaactcggt caccgagaga ttacgataaa tttggctgcc gcgcgaggtt agcggagcga
      901 agcccggcat gaagaaatgc agtcgcggga agggcaccat gttgacggcc agtttacgaa
      961 gatccgcgtt cagttgcccg gggaagcgca gacaggtagt cacaccggac attgtcattg
     1021 acacgagatg attgaggtcg ccataggtgg gagtggtcag tttgagggtg cggaaacaga
     1081 tgtcgtacaa agcttcgttg tcgatacaga aggtctcatc ggtgttctcc actaactggt
     1141 gaacggaaag ggtggcgttg tatggttcaa ccactgtgtc ggaaaccttg ggagatggga
     1201 cgacactgaa cgacatcatg atgcggtcgg ggtattcttc gcggatcttg gagatcatca
     1261 gcgttcccat tccggatccc gtgccaccac ccagggaatg cgccagttgg aagccctgca
     1321 ggcagtcgca actctccgct tcacgccgga ccacatccat caccgcgtcc accagttcgg
     1381 cgccctcggt gtagtggcct ttggcccagt tgttccccgc tccgctctga ccgaagataa
     1441 aattgtccgg gcgaaatagt tgaccgaaag tcgaagaccg gaccgagtcc atcgttcctg
     1501 gctccagatc caccagaact gcacgcggca catatttccc gccgccagct tcgttgtaat
     1561 agacattgac gcgttctaac tgcaagtccg aagtcccgcg gtaagatcca gttggatcga
     1621 tgccgtgctc gtccgatatc acttcccaaa actttgatcc aatttggttc ccgcactgac
     1681 cggcctggag atggacaatc tccctcatgg ttaccttgtt tagctatctt tctagaagat
     1741 ctcctacaat attctcagct gccatggaaa atcgatgttc ttcttttatt ctctcaagat
     1801 tttcaggctg tatattaaaa cttatattaa gaactatgct aaccacctca tcaggaaccg
     1861 ttgtaggtgg cgtgggtttt cttggcaatc gactctcatg aaaactacga gctaaatatt
     1921 caatatgttc ctcttgacca actttattct gcattttttt tgaacgaggt ttagagcaag
     1981 cttcaggaaa ctgagacagg aattttatta aaaatttaaa ttttgaagaa agttcagggt
     2041 taatagcatc cattttttgc tttgcaagtt cctcagcatt cttaacaaaa gacgtctctt
     2101 ttgacatgtt taaagtttaa acctcctgtg tgaaattatt atccgctcat aattccacac
     2161 attatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     2221 acattaattg cgttgcgctc actgccaatt gctttccagt cgggaaacct gtcgtgccag
     2281 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     2341 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2401 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     2461 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2521 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2581 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     2641 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     2701 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     2761 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     2821 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     2881 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2941 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3001 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3061 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3121 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3181 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     3241 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     3301 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     3361 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     3421 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     3481 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     3541 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     3601 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     3661 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     3721 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     3781 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     3841 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     3901 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     3961 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     4021 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     4081 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     4141 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     4201 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     4261 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     4321 acgaggcc
//
