LOCUS       sequence-399766-        9167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of TagRFP-T-tagged Galectin 3 with mutation of 
            carbohdrate recognition binding domain (CRD) (R186S mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9167)
  AUTHORS   Roman-Fernandez A, Mansour MA, Kugeratski FG, Anand J, Sandilands E,
            Galbraith L, Rakovic K, Freckmann EC, Cumming EM, Park J, Nikolatou 
            K, Lilla S, Shaw R, Strachan D, Mason S, Patel R, McGarry L, Katoch 
            A, Campbell KJ, Nixon C, Miller CJ, Leung HY, Le Quesne J, Norman 
            JC, Zanivan S, Blyth K, Bryant DM
  TITLE     Spatial regulation of the glycocalyx component podocalyxin is a 
            switch for prometastatic function.
  JOURNAL   Sci Adv. 2023 Feb 3;9(5):eabq1858. doi: 10.1126/sciadv.abq1858. Epub
            2023 Feb 3.
  PUBMED    36735782
REFERENCE   2  (bases 1 to 9167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-02-3"; volume: "9(5):eabq1858. doi"; pages: " 
            10.1126/sciadv.abq1858. Epub 2023 Feb 3"
COMMENT     Sequence Label: pLEX303 TagRFP-T LGALS3 R186S
FEATURES             Location/Qualifiers
     source          1..9167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             204..384
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    431..556
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1049..1282
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1467..1511
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1686..2186
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2105..2123
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2198..2593
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2653..2770
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2887..3190
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3191..3394
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3345..3365
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3433..3442
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3439..4170
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     misc_feature    4996..5584
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5049..5069)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5467..5478)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5656..5889
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5967..6088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6004..6023)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6058..6077
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6128..6263
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6283..6302)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6284..6302)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6312..6329)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6312..6328)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6321..6343)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6470..6925
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6557..6576)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6767..6788
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6951..7055
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7056..7124,7125..7916)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7274..7293)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8087..8675
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8576..8595
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8829..8846
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8963..8984
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8999..9004,9005..9022,9023..9029)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9037..9053
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9042..9064
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9061..9077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9061..9077
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9096..9116
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9098..9116
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        join(9144..9167,1..203)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
       61 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
      121 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
      181 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
      241 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      301 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      361 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      421 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      481 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      541 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
      601 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
      661 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
      721 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
      781 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
      841 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
      901 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
      961 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1021 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1081 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1141 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1201 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1261 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1321 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1381 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1441 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1501 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1561 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     1621 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     1681 gacccttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
     1741 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
     1801 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc
     1861 cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag
     1921 ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc
     1981 gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc
     2041 gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg
     2101 cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg
     2161 accgaatcac cgacctctct ccccaggggg taccaccatg gccaagcctt tgtctcaaga
     2221 agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga
     2281 ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt
     2341 atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc
     2401 ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag
     2461 cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt
     2521 gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta
     2581 tgtgtgggag ggcctgcagc tgcagtagta agaattctag atcttgagac aaatggcagt
     2641 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     2701 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     2761 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg
     2821 ggcccggggt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     2881 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2941 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     3001 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     3061 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     3121 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     3181 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     3241 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     3301 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     3361 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
     3421 gcgctaccgg tcgccaccat ggtgtctaag ggcgaagagc tgattaagga gaacatgcac
     3481 atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac atccgagggc
     3541 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct
     3601 ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcag aaccttcatc
     3661 aaccacaccc agggcatccc cgatttcttt aagcagtcct tccctgaggg cttcacatgg
     3721 gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc
     3781 caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttccc atccaacggc
     3841 cctgtgatgc agaagaaaac actcggctgg gaggccaaca ccgagatgct gtaccccgct
     3901 gacggcggcc tggaaggcag aaccgacatg gccctgaagc tcgtgggcgg gggccacctg
     3961 atctgcaact tcaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc
     4021 ggcgtctact atgtggacca cagactggaa agaatcaagg aggccgacaa agagacctac
     4081 gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa actggggcac
     4141 aaacttaatg gcatggacga gctgtacaag tccggactca gatctcgagc tcaagcttcg
     4201 aacatggcag acaatttttc gctccatgat gcgttatctg ggtctggaaa cccaaaccct
     4261 caaggatggc ctggcgcatg ggggaaccag cctgctgggg cagggggcta cccaggggct
     4321 tcctatcctg gggcctaccc cgggcaggca cccccagggg cttatcctgg acaggcacct
     4381 ccaggcgcct accctggagc acctggagct tatcccggag cacctgcacc tggagtctac
     4441 ccagggccac ccagcggccc tggggcctac ccatcttctg gacagccaag tgccccggga
     4501 gcctaccctg ccactggccc ctatggcgcc cctgctgggc cactgattgt gccttataac
     4561 ctgcctttgc ctgggggagt ggtgcctcgc atgctgataa caattctggg cacggtgaag
     4621 cccaatgcaa acagaattgc tttagatttc caaagaggga atgatgttgc cttccacttt
     4681 aatccccgat ttaacgagaa taacaggaga gtcattgttt gcaatacaaa gctggataat
     4741 aactggggaa gggaagaatc tcagtcggtt ttcccatttg aaagtgggaa accattcaaa
     4801 atacaagtac tggttgaacc tgaccacttc aaggttgcag tgaatgatgc tcacttgctc
     4861 caatataacc acagagtaaa aaaactcaat gaaatcagca aactgggaat ttctggtgac
     4921 atagacctca ccagtgcttc atataccatg atataaggat cccgtacgtg atcaccggtg
     4981 gcgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5041 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5101 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     5161 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     5221 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     5281 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     5341 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     5401 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     5461 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     5521 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     5581 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     5641 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     5701 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5761 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5821 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5881 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     5941 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     6001 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6061 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     6121 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6181 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     6241 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     6301 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     6361 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     6421 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     6481 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6541 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6601 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6661 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6721 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6781 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6841 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     6901 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6961 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     7021 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     7081 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     7141 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     7201 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     7261 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     7321 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     7381 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     7441 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7501 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7561 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     7621 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7681 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7741 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7801 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7861 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7921 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7981 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     8041 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8101 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     8161 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8221 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8281 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8341 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8401 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8461 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8521 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8581 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8641 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8701 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8761 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8821 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     8881 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     8941 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     9001 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     9061 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     9121 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgt
//
