LOCUS       sequence-399767-        4526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tardigrade (Hypsibius exemplaris) alpha-tubulin Tub1C1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Novotna Floriancicova K, Baltzis A, Smejkal J, Czernekova M, 
            Kaczmarek L, Maly J, Notredame C, Vinopal S
  TITLE     Phylogenetic and functional characterization of water bears 
            (Tardigrada) tubulins.
  JOURNAL   Sci Rep. 2023 Mar 30;13(1):5194. doi: 10.1038/s41598-023-31992-z.
  PUBMED    36997657
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-03-30"; volume: "13(1):5194. doi"; pages: " 
            10.1038/s41598-023-31992-z"
COMMENT     Sequence Label: pJet1.2-tHe-Tub1C1
FEATURES             Location/Qualifiers
     source          1..4526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(2358..2364,2365..2382,2383..2388))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    2403..2424
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2544..2561)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2715..3303)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2795..2814)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3474..4265,4266..4334))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4097..4116
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4335..4439)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4507..4525
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tcccatacag ctcacaagat tcccttctta atatcaaact atttgcggat
      421 ctatttcggc gacctcaaag aaaatgcgtg aatgtatctc aatccacgtc ggacaggctg
      481 gagtgcagat cggcaatgcc tgctgggaac tgtactgcct ggagcacggc atccagccgg
      541 acggacggat gtcgcagtcg aaggaaggcg gcaaacgtga cggccaagac tcattcacca
      601 ctttcttcag cgaaactggg tcaggcaaat acgttccccg agctgttttt gtggaccttg
      661 agcctactgt tgtcgacgaa gttcggacag gcacctacaa gcagcttttc catccggagc
      721 agctgattac cgggaaggag gatgccgcca acaactacgc tcgtggccat tacaccgtcg
      781 gcaaggagat tgtcgacgtc accattgacc ggatccggaa gttggctgac cagtgcactg
      841 gcctgcaggg atttcttatt tttcattcgt tcggcggtgg cactggttcg ggtttcactt
      901 cgttgctcat ggaacgcttg tctgtcgagt ttggcaagaa gtcaaagttg gagttttgcg
      961 tctaccccgc tccggaagtc tcgactgctg tggttgaacc gtacaattcc atcctcacca
     1021 cccacaccac cctggagcac tcggactgtg cattcatggt agacaatgaa gccatttttg
     1081 acatctgtcg ccggaaattg gatgtcgagc gaccgactta caccaacttg aaccggctca
     1141 tcagccagat cgtttcttcc atcacagcct cgctccgctt cgacggcgcc ctgaacgtcg
     1201 acctcacgga attccagacc aacttggtgc cgtatccgcg tatccacttc ccgctggtat
     1261 cttactcacc cgtcatttca gcggaaaagg cgttccacga gcagttgtcg gtccaagaga
     1321 tcaccaatgc ctgcttcgag ccggccaacc agatggtgaa gtgcgacccg cgccagggca
     1381 aatacatggc ctgctgcatg ctctaccgcg gtgatgtggt tccgaaggat gtcaccgcgg
     1441 ccatcgcgac cgtgaagagc aagtcgagca tccgtttcgt ggattggtgc ccgaccggtt
     1501 tcaaggttgg cttgaacttc cagccaccga ccgtcgtccc gggcgccgat caggcagccg
     1561 ttccgcgtgc cgtttgcatg ctttccaaca ccaccgccat cgtggaagcc tgggcgaggt
     1621 tgaaccacaa gttcgacctg atgtacgcca agcgcgcctt tgtccactgg tacgtcggcg
     1681 agggcatgga agagggcgag ttcagcgagg cccgagaaga tttggccgcc ctcgagaggg
     1741 actacgagga ggccggcgcg gactctaccg acggccagga cgacgaaaat ggcgaattct
     1801 aaagccggta tcaccagagc tgtatttttg tcgaactttc ggattttcgt cgctatgttt
     1861 tcgtttttat cctgtcctat gttttctaat taatatactt gttttatcat gggaagcgag
     1921 tggatctttc tagaagatct cctacaatat tctcagctgc catggaaaat cgatgttctt
     1981 cttttattct ctcaagattt tcaggctgta tattaaaact tatattaaga actatgctaa
     2041 ccacctcatc aggaaccgtt gtaggtggcg tgggttttct tggcaatcga ctctcatgaa
     2101 aactacgagc taaatattca atatgttcct cttgaccaac tttattctgc attttttttg
     2161 aacgaggttt agagcaagct tcaggaaact gagacaggaa ttttattaaa aatttaaatt
     2221 ttgaagaaag ttcagggtta atagcatcca ttttttgctt tgcaagttcc tcagcattct
     2281 taacaaaaga cgtctctttt gacatgttta aagtttaaac ctcctgtgtg aaattattat
     2341 ccgctcataa ttccacacat tatacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2401 taatgagtga gctaactcac attaattgcg ttgcgctcac tgccaattgc tttccagtcg
     2461 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     2521 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     2581 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2641 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2701 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2761 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2821 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2881 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2941 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3001 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3061 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3121 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3181 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3241 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3301 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3361 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3421 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     3481 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3541 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3601 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3661 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3721 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3781 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3841 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3901 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3961 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     4021 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     4081 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4141 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4201 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     4261 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     4321 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     4381 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     4441 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     4501 tataaaaata ggcgtatcac gaggcc
//
