LOCUS       sequence-399777-        6842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6842)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 6842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pDESTmycYBX1
FEATURES             Location/Qualifiers
     source          1..6842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6..26)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      191..326
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             391..1185
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(445..464)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1055..1074
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1249..1297
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(1338..1357)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1457..1479
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        1603..1707
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1708..1776,1777..2568)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1926..1945)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2739..3327
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3228..3247
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3481..3498
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        complement(3572..3676)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        3786..4089
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4090..4293
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4244..4264
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4290..4314
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4410..4439
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    4449..4473
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5486..5510)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5542..5561)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5543..5561)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5572..5589)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5572..5588)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5581..5603)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    5993..6127
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6030..6049)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6084..6103
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6253..6708
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6340..6359)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6550..6571
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        join(6825..6842,1..340)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        join(6825..6842,1..174)
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
ORIGIN
        1 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
       61 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
      121 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
      181 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
      241 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
      301 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttgatt cttctgacac
      361 aacagtctcg aacttaaggc tagagccacc atgattgaac aagatggatt gcacgcaggt
      421 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
      481 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
      541 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
      601 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
      661 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
      721 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
      781 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
      841 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
      901 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
      961 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     1021 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     1081 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     1141 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     1201 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg atggccgcaa taaaatatct
     1261 ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagcgat aaggatccgc
     1321 gtatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     1381 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     1441 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     1501 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     1561 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     1621 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     1681 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     1741 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     1801 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     1861 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     1921 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     1981 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     2041 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     2101 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     2161 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     2221 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     2281 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     2341 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     2401 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     2461 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     2521 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     2581 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     2641 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2701 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2761 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2821 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2881 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2941 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3001 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3061 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3121 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3181 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3241 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3301 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3361 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3421 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3481 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     3541 cgttggccga ttcattaatg cagagcttgc aattcgcgct ttttcaatat tattgaagca
     3601 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3661 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     3721 ttatcatgac attaacctat aaaaataggc gtattacgag gccctttcac gcattagatg
     3781 catgtcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     3841 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     3901 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     3961 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     4021 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     4081 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     4141 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     4201 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     4261 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
     4321 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
     4381 actctagcct aggccgcggt accaccatgg aacaaaaact tatttctgaa gaagatctgt
     4441 ctagatcaac aagtttgtac aaaaaagcag gctccaccat gggaaccatg agcagcgagg
     4501 ccgagaccca gcagccgccc gccgcccccc ccgccgcccc cgccctcagc gccgccgaca
     4561 ccaagcccgg cactacgggc agcggcgcag ggagcggtgg cccgggcggc ctcacatcgg
     4621 cggcgcctgc cggcggggac aagaaggtca tcgcaacgaa ggttttggga acagtaaaat
     4681 ggttcaatgt aaggaacgga tatggtttca tcaacaggaa tgacaccaag gaagatgtat
     4741 ttgtacacca gactgccata aagaagaata accccaggaa gtaccttcgc agtgtaggag
     4801 atggagagac tgtggagttt gatgttgttg aaggagaaaa gggtgcggag gcagcaaatg
     4861 ttacaggtcc tggtggtgtt ccagttcaag gcagtaaata tgcagcagac cgtaaccatt
     4921 atagacgcta tccacgtcgt aggggtcctc cacgcaatta ccagcaaaat taccagaata
     4981 gtgagagtgg ggaaaagaac gagggatcgg agagtgctcc cgaaggccag gcccaacaac
     5041 gccggcccta ccgcaggcga aggttcccac cttactacat gcggagaccc tatgggcgtc
     5101 gaccacagta ttccaaccct cctgtgcagg gagaagtgat ggagggtgct gacaaccagg
     5161 gtgcaggaga acaaggtaga ccagtgaggc agaatatgta tcggggatat agaccacgat
     5221 tccgcagggg ccctcctcgc caaagacagc ctagagagga cggcaatgaa gaagataaag
     5281 aaaatcaagg agatgagacc caaggtcagc agccacctca acgtcggtac cgccgcaact
     5341 tcaattaccg acgcagacgc ccagaaaacc ctaaaccaca agatggcaaa gagacaaaag
     5401 cagccgatcc accagctgag aattcgtccg ctcccgaggc tgagcagggc ggggctgagt
     5461 aagcggccgc actcgagata tctagaccca gctttcttgt acaaagtggt tgatcgcgtg
     5521 catgcgacgt catagctctc tccctatagt gagtcgtatt ataagctagg cactggccgt
     5581 cgttttacaa cgtcgtgact gggaaaactg ctagcttggg atctttgtga aggaacctta
     5641 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
     5701 tataaaattt ttaagtgtat aatgtgttaa actagctgca tatgcttgct gcttgagagt
     5761 tttgcttact gagtatgatt tatgaaaata ttatacacag gagctagtga ttctaattgt
     5821 ttgtgtattt tagattcaca gtcccaaggc tcatttcagg cccctcagtc ctcacagtct
     5881 gttcatgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     5941 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     6001 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6061 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6121 ctggatcgat cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6181 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     6241 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6301 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6361 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6421 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     6481 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6541 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     6601 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6661 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta
     6721 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgcggatctg cgcagcacca
     6781 tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac
     6841 ca
//
