LOCUS       sequence-399856-        2554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Target plasmid containing attachement site for ZooTsy..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Faure G, Saito M, Benler S, Peng I, Wolf YI, Strecker J, Altae-Tran 
            H, Neumann E, Li D, Makarova KS, Macrae RK, Koonin EV, Zhang F
  TITLE     Modularity and diversity of target selectors in Tn7 transposons.
  JOURNAL   Mol Cell. 2023 Jun 15;83(12):2122-2136.e10. doi: 
            10.1016/j.molcel.2023.05.013. Epub 2023 Jun 1.
  PUBMED    37267947
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.05.013"; journalName: "Mol Cell"; date: 
            "2023-06-15- 15"; volume: "83"; issue: "12"; pages: "2122-2136.e10"
COMMENT     Sequence Label: pSCT81: pTarget(ZooTsy)
FEATURES             Location/Qualifiers
     source          1..2554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(215..233)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      229..276
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(497..1156)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1071..1090
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1157..1259)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1651..1668)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1785..2330)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1865..1884)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cacggcttcg accgcagcac tggtcggtgg aggcggcacg ccccgccccg gcgagatcag
       61 cctggcgcac aacggcgtgc tgtacctgga cgagctgccg gaattcgacc gacgtgtgct
      121 cgaggccctg cgcgagccgc tggaaagcgg ccacatcacg atttcccgtg cagcccgccg
      181 cgccgagttc ccggcccgct ctaggctgct gccaccgctg agcaataact agcataaccc
      241 cttggggcct ctaaacgggt cttgaggggt tttttgctga aacctcaggc atttgagaag
      301 cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg
      361 gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc
      421 cattcatccg cttattatca cttattcagg cgtagcaacc aggcgtttaa gggcaccaat
      481 aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
      541 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
      601 gcatcagcac cttgtcgcct tgcgtataat atttgcccat agtgaaaacg ggggcgaaga
      661 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
      721 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
      781 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
      841 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
      901 cccatatcac cagctcaccg tctttcattg ccatacggaa ctccggatga gcattcatca
      961 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     1021 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     1081 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     1141 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     1201 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa
     1261 cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat
     1321 ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgcaa agtgcgtcgg
     1381 gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct
     1441 tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa
     1501 agcaccgccg gacatcagcg ctagcggagt gtatactggc ttactatgtt ggcactgatg
     1561 agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca
     1621 gaatatgtga tacaggatat attccgcttc ctcgctcact gactcgctac gctcggtcgt
     1681 tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg aagatgccag
     1741 gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca taggctccgc
     1801 ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga
     1861 ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc tgttcctgcc
     1921 tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac
     1981 actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca
     2041 gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacatgc
     2101 aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc ttgaagtcat
     2161 gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct ccaagccagt
     2221 tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg
     2281 ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa gaagatcatc
     2341 ttattaatca gataaaatat ttctagattt cagtgcaatt tatctcttca aatgtagcac
     2401 ctgaagtcag ccccatacga tataagttgt aattctcatg ttagtcatgc cccgcgccca
     2461 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     2521 atgagtgagc taacttacat taattgcgtt gcgc
//
