LOCUS       sequence-399859-        2387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ZooTsy donor harboring KanR cargo..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Faure G, Saito M, Benler S, Peng I, Wolf YI, Strecker J, Altae-Tran 
            H, Neumann E, Li D, Makarova KS, Macrae RK, Koonin EV, Zhang F
  TITLE     Modularity and diversity of target selectors in Tn7 transposons.
  JOURNAL   Mol Cell. 2023 Jun 15;83(12):2122-2136.e10. doi: 
            10.1016/j.molcel.2023.05.013. Epub 2023 Jun 1.
  PUBMED    37267947
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.05.013"; journalName: "Mol Cell"; date: 
            "2023-06-15- 15"; volume: "83"; issue: "12"; pages: "2122-2136.e10"
COMMENT     Sequence Label: pSCT221: pR6K-Donor(ZooTsy)_H1:12_E1:135_E2:20...
FEATURES             Location/Qualifiers
     source          1..2387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     15..31
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15..31
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(205..999)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(316..335)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     926..945
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     protein_bind    1386..1433
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(1660..1676)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     oriT            1813..1921
                     /note="incP origin of transfer"
     rep_origin      1972..2328
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gagagtgcac catacaggaa acagctatga ccatgattac gccaagctcg gcgcgccatt
       61 gggattcggt cgaattcgga ctgggattct ccaatataga gcgcttttga agctgatgtg
      121 cttaaaaact tactcaatgg aataattcta gataattcac tagtgattgg aaatgttgga
      181 tcctcatact cttccttttt caattcagaa gaactcgtca agaaggcgat agaaggcgat
      241 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
      301 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
      361 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
      421 caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag
      481 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
      541 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
      601 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
      661 tactttctcg gcaggagcaa ggtgggatga caggagatcc tgccccggca cttcgcccaa
      721 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
      781 cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga
      841 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
      901 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
      961 ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt
     1021 ctcttgatca gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca
     1081 gtttactttg cagggcttcc caaccttacc agagggcgcc ccagctggca attccggttc
     1141 gcttgctgtc cataaaaccg cccagtctag ctatcgccat gtaagcccac tgcaagctac
     1201 ctgctttctc tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat
     1261 ccggggtcag caccgtttct gcggactggc tttctacgtg ttccgcttcc tttagcagcc
     1321 cttgcgccat gcatttgtta aatcgaatta tccattgctg ttgacaaagg gaatcagggg
     1381 atcttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc
     1441 ttttgaagct gattcgagat catgcatgag ctcactagtg gatccccccg actacttcta
     1501 atgctgtgac atcactcaca ggattcctgc aagtaatgcg acattggata ctttgggatt
     1561 cctttatagt cagtgggtta tccgaatttt tgccttgttc gttgctacat tggctaaatc
     1621 agaacttcgg cagctcgtca tcccaatacg cgtcaattca ctggccgtcg ttttactact
     1681 ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt
     1741 tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca
     1801 acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt
     1861 cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc
     1921 gataagctct gataacgcag gaaagaacat gggcagttca acctgttgat agtacgtact
     1981 aagctctcat gtttcacgta ctaagctctc aagtttaacg tactaagctc tcatgtttaa
     2041 cgaactaaac cctcatggct aacgtactaa gctctcatgg ctaacgtact aagctctcat
     2101 gtttcacgta ctaagctctc atgtttgaac aataaaatta atataaatca gcaacttaaa
     2161 tagcctctaa ggttttaagt tttataagaa aaaaaagaat atataaggct tttaaagctt
     2221 ttaaggttta acggttgtgg acaacaagcc agggatgtaa cgcactgaga agcccttaga
     2281 gcctctcaaa gcaattttga gtgacacagg aacacttaac ggctgacaac cggctggtaa
     2341 caggattagc agagcgaggt atgtaggcgg tgctacaaga ttgtact
//
