LOCUS       sequence-399864-        2571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AjTn6022 donor harboring KanR cargo..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Faure G, Saito M, Benler S, Peng I, Wolf YI, Strecker J, Altae-Tran 
            H, Neumann E, Li D, Makarova KS, Macrae RK, Koonin EV, Zhang F
  TITLE     Modularity and diversity of target selectors in Tn7 transposons.
  JOURNAL   Mol Cell. 2023 Jun 15;83(12):2122-2136.e10. doi: 
            10.1016/j.molcel.2023.05.013. Epub 2023 Jun 1.
  PUBMED    37267947
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.05.013"; journalName: "Mol Cell"; date: 
            "2023-06-15- 15"; volume: "83"; issue: "12"; pages: "2122-2136.e10"
COMMENT     Sequence Label: pSCT91: pR6K-Donor(AjTn6022)
FEATURES             Location/Qualifiers
     source          1..2571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            363..471
                     /note="incP origin of transfer"
     primer_bind     608..624
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(857..904)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             1291..2085
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1345..1364)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1955..1974
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2437..2453)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2437..2453)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      join(2527..2571,1..312)
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac cgttaaacct
       61 taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa accttagagg
      121 ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct tagtacgtga
      181 aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg agggtttagt
      241 tcgttaaaca tgagagctta gtacgttaaa cttgagagct tagtacgtga aacatgagag
      301 cttagtacgt actatcaaca ggttgaactg cccatgttct ttcctgcgtt atcagagctt
      361 atcggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca
      421 gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc
      481 cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg
      541 aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg
      601 gaaagtagta aaacgacggc cagtgaattg acgcgtattg ggattgtcat ttacagcaat
      661 agataagagt gtcatgataa gaaaacaata ggttgccact ctaaaattac acaaatagat
      721 tagagtaatg acagtaataa gcaagagtgg cttgcaaatt tacacaaata cgcacgagtg
      781 gctgtaagag gcattctgtc ggggggatcc actagtgagc tcatgcatga tctcgaatta
      841 gcttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa
      901 cttcaagatc ccctgattcc ctttgtcaac agcaatggat aattcgattt aacaaatgca
      961 tggcgcaagg gctgctaaag gaagcggaac acgtagaaag ccagtccgca gaaacggtgc
     1021 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag
     1081 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg
     1141 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
     1201 aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct
     1261 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     1321 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     1381 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     1441 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     1501 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     1561 tggctgctat tgggcgaagt gccggggcag gatctcctgt catcccacct tgctcctgcc
     1621 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     1681 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     1741 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     1801 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     1861 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     1921 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     1981 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     2041 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaattga aaaaggaaga
     2101 gtatgaggat ccaacatttc caatcactag tgaattatct agaattattc cattgagtaa
     2161 gtttttaagc acatcagctt caaaagcgct cttcactgcc ctcattccta agaaacccac
     2221 catatctata gacagttctg cgaatattat catttgggaa acaatcaata gttgtgactt
     2281 taactttttt atttgcaggc atatctttta gtgaatttaa tgaaattcat atataaatag
     2341 tatagttttt attatcactc tagtttattt gtgtaaactc tgatttattt gtggaactga
     2401 caatcccaat ggcgcgccga gcttggcgta atcatggtca tagctgtttc ctgtatggtg
     2461 cactctcagt acaatcttgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2521 gccggttgtc agccgttaag tgttcctgtg tcactcaaaa ttgctttgag a
//
