LOCUS       sequence-399901-        4492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Complementation of M. smegmatis MceG(∆270-360).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert D
  TITLE     Structure of an endogenous mycobacterial MCE lipid transporter.
  JOURNAL   Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi: 
            10.21203/rs.3.rs-2412186/v1. Preprint.
  PUBMED    36711512
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Res Sq. 
            2023 Jan 10:rs.3.rs-2412186. doi: 10.21203/rs.3.rs-2412186/v1. 
            Preprint."
COMMENT     Sequence Label: pBEL2711
FEATURES             Location/Qualifiers
     source          1..4492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2185..2231
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2270..2317
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2336..2710
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYTEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      2762..3350
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3251..3270
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgagtgccac gcgcgaaacg ccgaatttcc gtgcaacgac gtgtgtcccg gaccgctacc
       61 ggacagcgag gtccgcgcca tcgccaacag catttggcgt tggatcacaa ccaagtcgcg
      121 catttgggcg gacgggatcg tggtctacga ggccacactc agtgcgcgcc agtcggccat
      181 ctcgcggaag ggcgcagcag cgcgcacggc ggcgagcaca gttgcgcggc gcgcaaagtc
      241 cgcgtcagcc atggaggcat tgctatgagc gacggctaca gcgacggcta caaccggcag
      301 ccgactgtcc gcaaaaagcg gcgcgtgacc gccgccgaag gcgctcgaat caccggacta
      361 tccgaacgcc acgtcgtccg gctcgtggcg caggaacgca gcgagtggct cgccgagcag
      421 gctgcacgcc gcgaacgcat ccgcgcctat cacgacgacg agggccactc ttggccgcaa
      481 acggccaaac atttcgggct gcatctggac accgttaagc gactcggcta tcgggcgagg
      541 aaagagcgtg cggcagaaca ggaagcggct caaaaggccc acaacgaagc cgacaatcca
      601 ccgctgttct aacgcaattg gggagcgggt gtcgcggggg ttccgtgggg ggttccgttg
      661 caacgggtcg gacaggtaaa agtcctggta gacgctagtt ttctggtttg ggccatgcct
      721 gtctcgttgc gtgtttcgtt gcgcccgttt tgaataccag ccagacgaga cggggttcta
      781 cgaatcttgg tcgataccaa gccatttccg ctgaatatcg gggagctcac cgccagaatc
      841 ggtggttgtg gtgatgtacg tggcgaactc cgttgtagtg cctgtggtgg catccgtggc
      901 gcggccgcgg taccagatct ttaaatctag acggtgacca caacgcgccc gctttgatcg
      961 gggacgtctg cggccgacca tttacgggtc ttgttgtcgt tggcggtcat gggccgaaca
     1021 tactcacccg gatcggaggg ccgaggacaa ggtcgaacga ggggcatgac ccggtgcggg
     1081 gcttcttgca ctcggcatag gcgagtgcta agaataacgt tggcactcgc gaccggtgag
     1141 tgctaggtcg ggacggtgag gccaggcccg tcgtcgcagc gagtggcagc gaggacaact
     1201 tgagccgtcc gtcgcgggca ctgcgcccgg ccagcgtaag tagcggggtt gccgtcaccc
     1261 ggtgaccccc gtttcatccc cgatccggag gaatcacttc gcaatgggcg tccaaatcga
     1321 cgtgacgggg ttgagcaagt ctttcgggtc gtcaaagatc tgggaagacg tgaccatgtc
     1381 gatcccggcc ggcgaggtga gcgtgctgct gggcccctcc ggtaccggca aatccgtgtt
     1441 cctgaagtcg ctgatcggtt tgctccgccc cgaacgtggc tccatcgtca tcgacggcac
     1501 cgacatcctg cagtgctcgg ccaaggagct ctacgagatc cgcacgctgt tcggtgtgct
     1561 gttccaggac ggcgcgctgt tcggctcgat gaacatctac gacaacaccg cgttcccgct
     1621 ccgtgagcac accaagaaga gcgagagcga aatccgcaag atcgtgatgg agaagctcga
     1681 cctggtcggt atgcccaacg acgggcacaa gttccccggt gagatctccg gcggtatgcg
     1741 caagcgcgcc ggtctggccc gcgcactcgt gctcgatccg gagatcatcc tctgcgacga
     1801 gccggactcg ggtctggacc cggtgcgtac ggcctacctg tcgcagctgc tgatcgacat
     1861 caacgcgcag atcgacgcga ccgtgctgat cgtgacgcac aacatcaaca tcgcccgcac
     1921 cgtgcccgac aacatgggca tgctgttccg caagcagctg gtgatgttcg gcccgcgtga
     1981 ggtgcttctg accagcgagg agccggtggt caagcagttc ctcaacggcc gccgtatcgg
     2041 cccgatcggt atgtcggagg agaaggacga ggccaccgcg gccgccgagc aggccctggt
     2101 ggacgccggc taagatccag ctgcagaatt cgaagcttat cgatgtcgac gtagttaact
     2161 agcgtacgat cgactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     2221 tttcgtttta tgccatcatg gccgcggtga tcagctagct cgagcacgtg ttgacaatta
     2281 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
     2341 taagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     2401 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     2461 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     2521 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac accgagtggt cggaggtcgt
     2581 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     2641 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     2701 gcaggactga gaatctagtt ccatgagcgt cagaccccgt agaaaagatc aaaggatctt
     2761 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2821 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2881 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     2941 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3001 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3061 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3121 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgagg
     3181 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3241 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3301 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3361 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3421 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3481 gccgcagccg aacgaccgag cgcaacgcgt gagcccacca gctccgtaag ttcgggcgct
     3541 gtgtggctcg tacccgcgca ttcaggcggc agggggtcta acgggtctaa ggcggcgtgt
     3601 acggccgcca cagcggctct cagcggcccg gaaacgtcct cgaaacgacg catgtgttcc
     3661 tcctggttgg tacaggtggt tgggggtgct cggctgtcgc tggtgttcca ccaccagggc
     3721 tcgacgggag agcgggggag tgtgcagttg tggggtggcc cctcagcgaa atatctgact
     3781 tggagctcgt gtcggaccat acaccggtga ttaatcgtgg tctactacca agcgtgagcc
     3841 acgtcgccga cgaatttgag cagctctggc tgccgtactg gccgctggca agcgacgatc
     3901 tgctcgaggg gatctaccgc caaagccgcg cgtcggccct aggccgccgg tacatcgagg
     3961 cgaacccaac agcgctggca aacctgctgg tcgtggacgt agaccatcca gacgcagcgc
     4021 tccgagcgct cagcgcccgg gggtcccatc cgctgcccaa cgcgatcgtg ggcaatcgcg
     4081 ccaacggcca cgcacacgca gtgtgggcac tcaacgcccc tgttccacgc accgaatacg
     4141 cgcggcgtaa gccgctcgca tacatggcgg cgtgcgccga aggccttcgg cgcgccgtcg
     4201 atggcgaccg cagttactca ggcctcatga ccaaaaaccc cggccacatc gcctgggaaa
     4261 cggaatggct ccactcagat ctctacacac tcagccacat cgaggccgag ctcggcgcga
     4321 acatgccacc gccgcgctgg cgtcagcaga ccacgtacaa agcggctccg acgccgctag
     4381 ggcggaattg cgcactgttc gattccgtca ggttgtgggc ctatcgtccc gccctcatgc
     4441 ggatctacct gccgacccgg aacgtggacg gactcggccg cgcgatctat gc
//
