LOCUS       sequence-399907-        4669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Complementation of M. smegmatis MceG(∆328-360).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert D
  TITLE     Structure of an endogenous mycobacterial MCE lipid transporter.
  JOURNAL   Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi: 
            10.21203/rs.3.rs-2412186/v1. Preprint.
  PUBMED    36711512
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Res Sq. 
            2023 Jan 10:rs.3.rs-2412186. doi: 10.21203/rs.3.rs-2412186/v1. 
            Preprint."
COMMENT     Sequence Label: pBEL2708
FEATURES             Location/Qualifiers
     source          1..4669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2617..2663
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2702..2749
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2768..3142
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYTEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      3194..3782
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3683..3702
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccccggccac atcgcctggg aaacggaatg gctccactca gatctctaca cactcagcca
       61 catcgaggcc gagctcggcg cgaacatgcc accgccgcgc tggcgtcagc agaccacgta
      121 caaagcggct ccgacgccgc tagggcggaa ttgcgcactg ttcgattccg tcaggttgtg
      181 ggcctatcgt cccgccctca tgcggatcta cctgccgacc cggaacgtgg acggactcgg
      241 ccgcgcgatc tatgccgagt gccacgcgcg aaacgccgaa tttccgtgca acgacgtgtg
      301 tcccggaccg ctaccggaca gcgaggtccg cgccatcgcc aacagcattt ggcgttggat
      361 cacaaccaag tcgcgcattt gggcggacgg gatcgtggtc tacgaggcca cactcagtgc
      421 gcgccagtcg gccatctcgc ggaagggcgc agcagcgcgc acggcggcga gcacagttgc
      481 gcggcgcgca aagtccgcgt cagccatgga ggcattgcta tgagcgacgg ctacagcgac
      541 ggctacaacc ggcagccgac tgtccgcaaa aagcggcgcg tgaccgccgc cgaaggcgct
      601 cgaatcaccg gactatccga acgccacgtc gtccggctcg tggcgcagga acgcagcgag
      661 tggctcgccg agcaggctgc acgccgcgaa cgcatccgcg cctatcacga cgacgagggc
      721 cactcttggc cgcaaacggc caaacatttc gggctgcatc tggacaccgt taagcgactc
      781 ggctatcggg cgaggaaaga gcgtgcggca gaacaggaag cggctcaaaa ggcccacaac
      841 gaagccgaca atccaccgct gttctaacgc aattggggag cgggtgtcgc gggggttccg
      901 tggggggttc cgttgcaacg ggtcggacag gtaaaagtcc tggtagacgc tagttttctg
      961 gtttgggcca tgcctgtctc gttgcgtgtt tcgttgcgcc cgttttgaat accagccaga
     1021 cgagacgggg ttctacgaat cttggtcgat accaagccat ttccgctgaa tatcggggag
     1081 ctcaccgcca gaatcggtgg ttgtggtgat gtacgtggcg aactccgttg tagtgcctgt
     1141 ggtggcatcc gtggcgcggc cgcggtacca gatctttaaa tctagacggt gaccacaacg
     1201 cgcccgcttt gatcggggac gtctgcggcc gaccatttac gggtcttgtt gtcgttggcg
     1261 gtcatgggcc gaacatactc acccggatcg gagggccgag gacaaggtcg aacgaggggc
     1321 atgacccggt gcggggcttc ttgcactcgg cataggcgag tgctaagaat aacgttggca
     1381 ctcgcgaccg gtgagtgcta ggtcgggacg gtgaggccag gcccgtcgtc gcagcgagtg
     1441 gcagcgagga caacttgagc cgtccgtcgc gggcactgcg cccggccagc gtaagtagcg
     1501 gggttgccgt cacccggtga cccccgtttc atccccgatc cggaggaatc acttcgcaat
     1561 gggcgtccaa atcgacgtga cggggttgag caagtctttc gggtcgtcaa agatctggga
     1621 agacgtgacc atgtcgatcc cggccggcga ggtgagcgtg ctgctgggcc cctccggtac
     1681 cggcaaatcc gtgttcctga agtcgctgat cggtttgctc cgccccgaac gtggctccat
     1741 cgtcatcgac ggcaccgaca tcctgcagtg ctcggccaag gagctctacg agatccgcac
     1801 gctgttcggt gtgctgttcc aggacggcgc gctgttcggc tcgatgaaca tctacgacaa
     1861 caccgcgttc ccgctccgtg agcacaccaa gaagagcgag agcgaaatcc gcaagatcgt
     1921 gatggagaag ctcgacctgg tcggtatgcc caacgacggg cacaagttcc ccggtgagat
     1981 ctccggcggt atgcgcaagc gcgccggtct ggcccgcgca ctcgtgctcg atccggagat
     2041 catcctctgc gacgagccgg actcgggtct ggacccggtg cgtacggcct acctgtcgca
     2101 gctgctgatc gacatcaacg cgcagatcga cgcgaccgtg ctgatcgtga cgcacaacat
     2161 caacatcgcc cgcaccgtgc ccgacaacat gggcatgctg ttccgcaagc agctggtgat
     2221 gttcggcccg cgtgaggtgc ttctgaccag cgaggagccg gtggtcaagc agttcctcaa
     2281 cggccgccgt atcggcccga tcggtatgtc ggaggagaag gacgaggcca ccgcggccgc
     2341 cgagcaggcc ctggtggacg ccggccagca cgacggcggt gtcgaggaga tcgagggtgt
     2401 gccgccgcag ctgcaggcca ccccgggtat gcccgagcgc aaggccgtgg cccgccgcaa
     2461 ggcccgcgtg cgcgagatcc tgcacaccct cccgcccgcc gcgcaggccg cgatcctcga
     2521 agagctcgac cgcgaccagc cgtaagatcc agctgcagaa ttcgaagctt atcgatgtcg
     2581 acgtagttaa ctagcgtacg atcgactgcc aggcatcaaa taaaacgaaa ggctcagtcg
     2641 aaagactggg cctttcgttt tatgccatca tggccgcggt gatcagctag ctcgagcacg
     2701 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     2761 ctaaaccatg gctaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     2821 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     2881 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     2941 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acaccgagtg
     3001 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     3061 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     3121 cgtggccgag gagcaggact gagaatctag ttccatgagc gtcagacccc gtagaaaaga
     3181 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3241 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3301 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     3361 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3421 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3481 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3541 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3601 cgcttcccga ggggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3661 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3721 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3781 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3841 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3901 ctgataccgc tcgccgcagc cgaacgaccg agcgcaacgc gtgagcccac cagctccgta
     3961 agttcgggcg ctgtgtggct cgtacccgcg cattcaggcg gcagggggtc taacgggtct
     4021 aaggcggcgt gtacggccgc cacagcggct ctcagcggcc cggaaacgtc ctcgaaacga
     4081 cgcatgtgtt cctcctggtt ggtacaggtg gttgggggtg ctcggctgtc gctggtgttc
     4141 caccaccagg gctcgacggg agagcggggg agtgtgcagt tgtggggtgg cccctcagcg
     4201 aaatatctga cttggagctc gtgtcggacc atacaccggt gattaatcgt ggtctactac
     4261 caagcgtgag ccacgtcgcc gacgaatttg agcagctctg gctgccgtac tggccgctgg
     4321 caagcgacga tctgctcgag gggatctacc gccaaagccg cgcgtcggcc ctaggccgcc
     4381 ggtacatcga ggcgaaccca acagcgctgg caaacctgct ggtcgtggac gtagaccatc
     4441 cagacgcagc gctccgagcg ctcagcgccc gggggtccca tccgctgccc aacgcgatcg
     4501 tgggcaatcg cgccaacggc cacgcacacg cagtgtgggc actcaacgcc cctgttccac
     4561 gcaccgaata cgcgcggcgt aagccgctcg catacatggc ggcgtgcgcc gaaggccttc
     4621 ggcgcgccgt cgatggcgac cgcagttact caggcctcat gaccaaaaa
//
