LOCUS       sequence-399909-        4408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Complementation of M. smegmatis MceG(∆242-360).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert D
  TITLE     Structure of an endogenous mycobacterial MCE lipid transporter.
  JOURNAL   Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi: 
            10.21203/rs.3.rs-2412186/v1. Preprint.
  PUBMED    36711512
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Res Sq. 
            2023 Jan 10:rs.3.rs-2412186. doi: 10.21203/rs.3.rs-2412186/v1. 
            Preprint."
COMMENT     Sequence Label: pBEL2713
FEATURES             Location/Qualifiers
     source          1..4408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2102..2148
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2187..2234
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2253..2627
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYTEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      2679..3267
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3168..3187
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgagtgcca cgcgcgaaac gccgaatttc cgtgcaacga cgtgtgtccc ggaccgctac
       61 cggacagcga ggtccgcgcc atcgccaaca gcatttggcg ttggatcaca accaagtcgc
      121 gcatttgggc ggacgggatc gtggtctacg aggccacact cagtgcgcgc cagtcggcca
      181 tctcgcggaa gggcgcagca gcgcgcacgg cggcgagcac agttgcgcgg cgcgcaaagt
      241 ccgcgtcagc catggaggca ttgctatgag cgacggctac agcgacggct acaaccggca
      301 gccgactgtc cgcaaaaagc ggcgcgtgac cgccgccgaa ggcgctcgaa tcaccggact
      361 atccgaacgc cacgtcgtcc ggctcgtggc gcaggaacgc agcgagtggc tcgccgagca
      421 ggctgcacgc cgcgaacgca tccgcgccta tcacgacgac gagggccact cttggccgca
      481 aacggccaaa catttcgggc tgcatctgga caccgttaag cgactcggct atcgggcgag
      541 gaaagagcgt gcggcagaac aggaagcggc tcaaaaggcc cacaacgaag ccgacaatcc
      601 accgctgttc taacgcaatt ggggagcggg tgtcgcgggg gttccgtggg gggttccgtt
      661 gcaacgggtc ggacaggtaa aagtcctggt agacgctagt tttctggttt gggccatgcc
      721 tgtctcgttg cgtgtttcgt tgcgcccgtt ttgaatacca gccagacgag acggggttct
      781 acgaatcttg gtcgatacca agccatttcc gctgaatatc ggggagctca ccgccagaat
      841 cggtggttgt ggtgatgtac gtggcgaact ccgttgtagt gcctgtggtg gcatccgtgg
      901 cgcggccgcg gtaccagatc tttaaatcta gacggtgacc acaacgcgcc cgctttgatc
      961 ggggacgtct gcggccgacc atttacgggt cttgttgtcg ttggcggtca tgggccgaac
     1021 atactcaccc ggatcggagg gccgaggaca aggtcgaacg aggggcatga cccggtgcgg
     1081 ggcttcttgc actcggcata ggcgagtgct aagaataacg ttggcactcg cgaccggtga
     1141 gtgctaggtc gggacggtga ggccaggccc gtcgtcgcag cgagtggcag cgaggacaac
     1201 ttgagccgtc cgtcgcgggc actgcgcccg gccagcgtaa gtagcggggt tgccgtcacc
     1261 cggtgacccc cgtttcatcc ccgatccgga ggaatcactt cgcaatgggc gtccaaatcg
     1321 acgtgacggg gttgagcaag tctttcgggt cgtcaaagat ctgggaagac gtgaccatgt
     1381 cgatcccggc cggcgaggtg agcgtgctgc tgggcccctc cggtaccggc aaatccgtgt
     1441 tcctgaagtc gctgatcggt ttgctccgcc ccgaacgtgg ctccatcgtc atcgacggca
     1501 ccgacatcct gcagtgctcg gccaaggagc tctacgagat ccgcacgctg ttcggtgtgc
     1561 tgttccagga cggcgcgctg ttcggctcga tgaacatcta cgacaacacc gcgttcccgc
     1621 tccgtgagca caccaagaag agcgagagcg aaatccgcaa gatcgtgatg gagaagctcg
     1681 acctggtcgg tatgcccaac gacgggcaca agttccccgg tgagatctcc ggcggtatgc
     1741 gcaagcgcgc cggtctggcc cgcgcactcg tgctcgatcc ggagatcatc ctctgcgacg
     1801 agccggactc gggtctggac ccggtgcgta cggcctacct gtcgcagctg ctgatcgaca
     1861 tcaacgcgca gatcgacgcg accgtgctga tcgtgacgca caacatcaac atcgcccgca
     1921 ccgtgcccga caacatgggc atgctgttcc gcaagcagct ggtgatgttc ggcccgcgtg
     1981 aggtgcttct gaccagcgag gagccggtgg tcaagcagtt cctcaactaa gatccagctg
     2041 cagaattcga agcttatcga tgtcgacgta gttaactagc gtacgatcga ctgccaggca
     2101 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatgc catcatggcc
     2161 gcggtgatca gctagctcga gcacgtgttg acaattaatc atcggcatag tatatcggca
     2221 tagtataata cgacaaggtg aggaactaaa ccatggctaa gttgaccagt gccgttccgg
     2281 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     2341 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     2401 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     2461 gcctggacga gctgtacacc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     2521 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     2581 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgagaa tctagttcca
     2641 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2701 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2761 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2821 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2881 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2941 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3001 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3061 cagcgtgagc tatgagaaag cgccacgctt cccgagggga gaaaggcgga caggtatccg
     3121 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3181 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3241 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3301 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3361 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3421 aacgcgtgag cccaccagct ccgtaagttc gggcgctgtg tggctcgtac ccgcgcattc
     3481 aggcggcagg gggtctaacg ggtctaaggc ggcgtgtacg gccgccacag cggctctcag
     3541 cggcccggaa acgtcctcga aacgacgcat gtgttcctcc tggttggtac aggtggttgg
     3601 gggtgctcgg ctgtcgctgg tgttccacca ccagggctcg acgggagagc gggggagtgt
     3661 gcagttgtgg ggtggcccct cagcgaaata tctgacttgg agctcgtgtc ggaccataca
     3721 ccggtgatta atcgtggtct actaccaagc gtgagccacg tcgccgacga atttgagcag
     3781 ctctggctgc cgtactggcc gctggcaagc gacgatctgc tcgaggggat ctaccgccaa
     3841 agccgcgcgt cggccctagg ccgccggtac atcgaggcga acccaacagc gctggcaaac
     3901 ctgctggtcg tggacgtaga ccatccagac gcagcgctcc gagcgctcag cgcccggggg
     3961 tcccatccgc tgcccaacgc gatcgtgggc aatcgcgcca acggccacgc acacgcagtg
     4021 tgggcactca acgcccctgt tccacgcacc gaatacgcgc ggcgtaagcc gctcgcatac
     4081 atggcggcgt gcgccgaagg ccttcggcgc gccgtcgatg gcgaccgcag ttactcaggc
     4141 ctcatgacca aaaaccccgg ccacatcgcc tgggaaacgg aatggctcca ctcagatctc
     4201 tacacactca gccacatcga ggccgagctc ggcgcgaaca tgccaccgcc gcgctggcgt
     4261 cagcagacca cgtacaaagc ggctccgacg ccgctagggc ggaattgcgc actgttcgat
     4321 tccgtcaggt tgtgggccta tcgtcccgcc ctcatgcgga tctacctgcc gacccggaac
     4381 gtggacggac tcggccgcgc gatctatg
//
