LOCUS       sequence-399910-       10326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CMP-PE-V1 in mammalian cells for prime editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10326)
  AUTHORS   Park SJ, Jeong TY, Shin SK, Yoon DE, Lim SY, Kim SP, Choi J, Lee H, 
            Hong JI, Ahn J, Seong JK, Kim K
  TITLE     Targeted mutagenesis in mouse cells and embryos using an enhanced 
            prime editor.
  JOURNAL   Genome Biol. 2021 Jun 3;22(1):170. doi: 10.1186/s13059-021-02389-w.
  PUBMED    34082781
REFERENCE   2  (bases 1 to 10326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2021-06-3"; volume: "22(1):170. doi"; pages: " 
            10.1186/s13059-021-02389-w"
COMMENT     Sequence Label: pCMV-CMP-PE-V1
FEATURES             Location/Qualifiers
     source          1..10326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             37..57
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(370..2883,2884..2886,2887..4470)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             370..4470
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6904..6924
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6933..6950
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6973..6990)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6979..7203
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7274..7290)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7274..7290)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7287..7309)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7298..7314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7322..7328,7329..7346,7347..7352))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7367..7388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7505..7522)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7676..8264)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7756..7775)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8435..9226,9227..9295))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9058..9077
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9296..9400)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9479..9498)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9670..10049
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10050..10253
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10204..10224
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     10295..10314
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10295..10313
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgaaacgga cagccgacgg aagcgagttc gagtcaccaa agaagaagcg gaaagtcatg
       61 cccaagagga aggtcagctc cgccgaaggc gccgccaagg aagagcccaa gaggagatcg
      121 gcgcggttgt cagctaaacc tcctgcaaaa gtggaagcga agccgaaaaa ggcagcagcg
      181 aaggataaat cttcagacaa aaaagtgcaa acaaaaggga aaaggggagc aaagggaaaa
      241 caggccgaag tggctaacca agaaactaaa gaagacttac ctgcggaaaa cggggaaacg
      301 aagactgagg agagtccagc ctctgatgaa gcaggagaga aagaagccaa gtctgatacc
      361 gggagcggag acaagaagta cagcatcggc ctggacatcg gcaccaactc tgtgggctgg
      421 gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc
      481 gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca
      541 gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg
      601 atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc
      661 cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc
      721 ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg
      781 agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg
      841 gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac
      901 agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa
      961 aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag
     1021 agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc
     1081 ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg
     1141 gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg
     1201 ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac
     1261 gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc
     1321 gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc
     1381 gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc
     1441 tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc
     1501 atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg
     1561 ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag
     1621 ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa
     1681 aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga
     1741 aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc
     1801 gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc
     1861 gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc
     1921 accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc
     1981 ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa
     2041 gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg
     2101 gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg
     2161 aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat
     2221 atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc
     2281 tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc
     2341 tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca
     2401 atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac
     2461 gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat
     2521 agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg
     2581 cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac
     2641 atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc
     2701 gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa
     2761 caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat
     2821 gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg
     2881 gacgctatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc
     2941 agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag
     3001 atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac
     3061 aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag
     3121 agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg
     3181 atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg
     3241 aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc
     3301 aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc
     3361 aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg
     3421 cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc
     3481 tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg
     3541 aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg
     3601 gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc
     3661 gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag
     3721 ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc
     3781 gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag
     3841 agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc
     3901 atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg
     3961 cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc
     4021 gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg
     4081 gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt
     4141 gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag
     4201 agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg
     4261 gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg
     4321 ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc
     4381 accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca
     4441 cggatcgacc tgtctcagct gggaggtgac ctggaggggg gcggaggctc ttccacagac
     4501 caccccaagt attcagacat gatcgtggct gccatccagg ccgagaagaa ccgcgctggc
     4561 tcctcgcgcc agtccattca gaagtatatc aagagccact acaaggtggg tgagaacgct
     4621 gactcgcaga tcaagttgtc catcaagcgc ctggtcacca ccggtgtcct caagcagacc
     4681 aaaggggtgg gggcctcggg gtccttccgg ctagccaaga gcgacgaacc ctctggagga
     4741 tctagcggag gatcctctgg cagcgagaca ccaggaacaa gcgagtcagc aacaccagag
     4801 agcagtggcg gcagcagcgg cggcagcagc accctaaata tagaagatga gtatcggcta
     4861 catgagacct caaaagagcc agatgtttct ctagggtcca catggctgtc tgattttcct
     4921 caggcctggg cggaaaccgg gggcatggga ctggcagttc gccaagctcc tctgatcata
     4981 cctctgaaag caacctctac ccccgtgtcc ataaaacaat accccatgtc acaagaagcc
     5041 agactgggga tcaagcccca catacagaga ctgttggacc agggaatact ggtaccctgc
     5101 cagtccccct ggaacacgcc cctgctaccc gttaagaaac cagggactaa tgattatagg
     5161 cctgtccagg atctgagaga agtcaacaag cgggtggaag acatccaccc caccgtgccc
     5221 aacccttaca acctcttgag cgggctccca ccgtcccacc agtggtacac tgtgcttgat
     5281 ttaaaggatg cctttttctg cctgagactc caccccacca gtcagcctct cttcgccttt
     5341 gagtggagag atccagagat gggaatctca ggacaattga cctggaccag actcccacag
     5401 ggtttcaaaa acagtcccac cctgtttaat gaggcactgc acagagacct agcagacttc
     5461 cggatccagc acccagactt gatcctgcta cagtacgtgg atgacttact gctggccgcc
     5521 acttctgagc tagactgcca acaaggtact cgggccctgt tacaaaccct agggaacctc
     5581 gggtatcggg cctcggccaa gaaagcccaa atttgccaga aacaggtcaa gtatctgggg
     5641 tatcttctaa aagagggtca gagatggctg actgaggcca gaaaagagac tgtgatgggg
     5701 cagcctactc cgaagacccc tcgacaacta agggagttcc tagggaaggc aggcttctgt
     5761 cgcctcttca tccctgggtt tgcagaaatg gcagcccccc tgtaccctct caccaaaccg
     5821 gggactctgt ttaattgggg cccagaccaa caaaaggcct atcaagaaat caagcaagct
     5881 cttctaactg ccccagccct ggggttgcca gatttgacta agccctttga actctttgtc
     5941 gacgagaagc agggctacgc caaaggtgtc ctaacgcaaa aactgggacc ttggcgtcgg
     6001 ccggtggcct acctgtccaa aaagctagac ccagtagcag ctgggtggcc cccttgccta
     6061 cggatggtag cagccattgc cgtactgaca aaggatgcag gcaagctaac catgggacag
     6121 ccactagtca ttctggcccc ccatgcagta gaggcactag tcaaacaacc ccccgaccgc
     6181 tggctttcca acgcccggat gactcactat caggccttgc ttttggacac ggaccgggtc
     6241 cagttcggac cggtggtagc cctgaacccg gctacgctgc tcccactgcc tgaggaaggg
     6301 ctgcaacaca actgccttga tatcctggcc gaagcccacg gaacccgacc cgacctaacg
     6361 gaccagccgc tcccagacgc cgaccacacc tggtacacgg atggaagcag tctcttacaa
     6421 gagggacagc gtaaggcggg agctgcggtg accaccgaga ccgaggtaat ctgggctaaa
     6481 gccctgccag ccgggacatc cgctcagcgg gctgaactga tagcactcac ccaggcccta
     6541 aagatggcag aaggtaagaa gctaaatgtt tatactgata gccgttatgc ttttgctact
     6601 gcccatatcc atggagaaat atacagaagg cgtgggtggc tcacatcaga aggcaaagag
     6661 atcaaaaata aagacgagat cttggcccta ctaaaagccc tctttctgcc caaaagactt
     6721 agcataatcc attgtccagg acatcaaaag ggacacagcg ccgaggctag aggcaaccgg
     6781 atggctgacc aagcggcccg aaaggcagcc atcacagaga ctccagacac ctctaccctc
     6841 ctcatagaaa attcatcacc ctctggcggc tcaaaaagaa ccgccgacgg cagcgaattc
     6901 gagcccaaga agaagaggaa agtctaaccg gtcatcatca ccatcaccat tgagtttaaa
     6961 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     7021 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgaga
     7081 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     7141 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     7201 tggcttctga ggcggaaaga accagctggg gctcgatacc gtcgacctct agctagagct
     7261 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7321 acaacatacg agccggaagc ataaagtgta aagcctaggg tgcctaatga gtgagctaac
     7381 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7441 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7501 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7561 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7621 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7681 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7741 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7801 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7861 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7921 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7981 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8041 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8101 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8161 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8221 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8281 tttctacggg gtctgacact cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8341 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8401 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8461 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8521 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8581 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8641 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8701 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8761 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8821 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8881 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8941 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9001 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9061 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9121 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9181 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9241 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9301 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9361 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9421 cacctgacgt cgacggatcg ggagatcgat ctcccgatcc cctagggtct actctcagta
     9481 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     9541 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     9601 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     9661 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     9721 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     9781 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     9841 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     9901 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     9961 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
    10021 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
    10081 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
    10141 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
    10201 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
    10261 tgaaccgtca gatccgctag agatccgcgg ccgctaatac gactcactat agggagagcc
    10321 gccacc
//
