LOCUS       sequence-399913-        4765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Complementation of M. smegmatis MceG(Y178A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert D
  TITLE     Structure of an endogenous mycobacterial MCE lipid transporter.
  JOURNAL   Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi: 
            10.21203/rs.3.rs-2412186/v1. Preprint.
  PUBMED    36711512
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Res Sq. 
            2023 Jan 10:rs.3.rs-2412186. doi: 10.21203/rs.3.rs-2412186/v1. 
            Preprint."
COMMENT     Sequence Label: pBEL2719
FEATURES             Location/Qualifiers
     source          1..4765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2006..2052
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2091..2138
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2157..2531
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYTEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      2583..3171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3072..3091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgacgacgag ggccactctt ggccgcaaac ggccaaacat ttcgggctgc atctggacac
       61 cgttaagcga ctcggctatc gggcgaggaa agagcgtgcg gcagaacagg aagcggctca
      121 aaaggcccac aacgaagccg acaatccacc gctgttctaa cgcaattggg gagcgggtgt
      181 cgcgggggtt ccgtgggggg ttccgttgca acgggtcgga caggtaaaag tcctggtaga
      241 cgctagtttt ctggtttggg ccatgcctgt ctcgttgcgt gtttcgttgc gcccgttttg
      301 aataccagcc agacgagacg gggttctacg aatcttggtc gataccaagc catttccgct
      361 gaatatcggg gagctcaccg ccagaatcgg tggttgtggt gatgtacgtg gcgaactccg
      421 ttgtagtgcc tgtggtggca tccgtggcgc ggccgcggta ccagatcttt aaatctagac
      481 ggtgaccaca acgcgcccgc tttgatcggg gacgtctgcg gccgaccatt tacgggtctt
      541 gttgtcgttg gcggtcatgg gccgaacata ctcacccgga tcggagggcc gaggacaagg
      601 tcgaacgagg ggcatgaccc ggtgcggggc ttcttgcact cggcataggc gagtgctaag
      661 aataacgttg gcactcgcga ccggtgagtg ctaggtcggg acggtgaggc caggcccgtc
      721 gtcgcagcga gtggcagcga ggacaacttg agccgtccgt cgcgggcact gcgcccggcc
      781 agcgtaagta gcggggttgc cgtcacccgg tgacccccgt ttcatccccg atccggagga
      841 atcacttcgc aatgggcgtc caaatcgacg tgacggggtt gagcaagtct ttcgggtcgt
      901 caaagatctg ggaagacgtg accatgtcga tcccggccgg cgaggtgagc gtgctgctgg
      961 gcccctccgg taccggcaaa tccgtgttcc tgaagtcgct gatcggtttg ctccgccccg
     1021 aacgtggctc catcgtcatc gacggcaccg acatcctgca gtgctcggcc aaggagctct
     1081 acgagatccg cacgctgttc ggtgtgctgt tccaggacgg cgcgctgttc ggctcgatga
     1141 acatctacga caacaccgcg ttcccgctcc gtgagcacac caagaagagc gagagcgaaa
     1201 tccgcaagat cgtgatggag aagctcgacc tggtcggtat gcccaacgac gggcacaagt
     1261 tccccggtga gatctccggc ggtatgcgca agcgcgccgg tctggcccgc gcactcgtgc
     1321 tcgatccgga gatcatcctc tgcgacgagc cggactcggg tctggacccg gtgcgtacgg
     1381 ccgccctgtc gcagctgctg atcgacatca acgcgcagat cgacgcgacc gtgctgatcg
     1441 tgacgcacaa catcaacatc gcccgcaccg tgcccgacaa catgggcatg ctgttccgca
     1501 agcagctggt gatgttcggc ccgcgtgagg tgcttctgac cagcgaggag ccggtggtca
     1561 agcagttcct caacggccgc cgtatcggcc cgatcggtat gtcggaggag aaggacgagg
     1621 ccaccgcggc cgccgagcag gccctggtgg acgccggcca gcacgacggc ggtgtcgagg
     1681 agatcgaggg tgtgccgccg cagctgcagg ccaccccggg tatgcccgag cgcaaggccg
     1741 tggcccgccg caaggcccgc gtgcgcgaga tcctgcacac cctcccgccc gccgcgcagg
     1801 ccgcgatcct cgaagagctc gaccgcgacc agccgcagct gtccgccccg accacccaga
     1861 cggccgccac cgcgcccgtc gagaactacg acgactcgcc gacgggcgtc atcgaggtgc
     1921 ccaagcaggc gtaagatcca gctgcagaat tcgaagctta tcgatgtcga cgtagttaac
     1981 tagcgtacga tcgactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     2041 ctttcgtttt atgccatcat ggccgcggtg atcagctagc tcgagcacgt gttgacaatt
     2101 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     2161 ctaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
     2221 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
     2281 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
     2341 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta caccgagtgg tcggaggtcg
     2401 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
     2461 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
     2521 agcaggactg agaatctagt tccatgagcg tcagaccccg tagaaaagat caaaggatct
     2581 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2641 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2701 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     2761 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2821 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2881 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2941 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgag
     3001 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3061 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3121 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3181 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3241 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3301 cgccgcagcc gaacgaccga gcgcaacgcg tgagcccacc agctccgtaa gttcgggcgc
     3361 tgtgtggctc gtacccgcgc attcaggcgg cagggggtct aacgggtcta aggcggcgtg
     3421 tacggccgcc acagcggctc tcagcggccc ggaaacgtcc tcgaaacgac gcatgtgttc
     3481 ctcctggttg gtacaggtgg ttgggggtgc tcggctgtcg ctggtgttcc accaccaggg
     3541 ctcgacggga gagcggggga gtgtgcagtt gtggggtggc ccctcagcga aatatctgac
     3601 ttggagctcg tgtcggacca tacaccggtg attaatcgtg gtctactacc aagcgtgagc
     3661 cacgtcgccg acgaatttga gcagctctgg ctgccgtact ggccgctggc aagcgacgat
     3721 ctgctcgagg ggatctaccg ccaaagccgc gcgtcggccc taggccgccg gtacatcgag
     3781 gcgaacccaa cagcgctggc aaacctgctg gtcgtggacg tagaccatcc agacgcagcg
     3841 ctccgagcgc tcagcgcccg ggggtcccat ccgctgccca acgcgatcgt gggcaatcgc
     3901 gccaacggcc acgcacacgc agtgtgggca ctcaacgccc ctgttccacg caccgaatac
     3961 gcgcggcgta agccgctcgc atacatggcg gcgtgcgccg aaggccttcg gcgcgccgtc
     4021 gatggcgacc gcagttactc aggcctcatg accaaaaacc ccggccacat cgcctgggaa
     4081 acggaatggc tccactcaga tctctacaca ctcagccaca tcgaggccga gctcggcgcg
     4141 aacatgccac cgccgcgctg gcgtcagcag accacgtaca aagcggctcc gacgccgcta
     4201 gggcggaatt gcgcactgtt cgattccgtc aggttgtggg cctatcgtcc cgccctcatg
     4261 cggatctacc tgccgacccg gaacgtggac ggactcggcc gcgcgatcta tgccgagtgc
     4321 cacgcgcgaa acgccgaatt tccgtgcaac gacgtgtgtc ccggaccgct accggacagc
     4381 gaggtccgcg ccatcgccaa cagcatttgg cgttggatca caaccaagtc gcgcatttgg
     4441 gcggacggga tcgtggtcta cgaggccaca ctcagtgcgc gccagtcggc catctcgcgg
     4501 aagggcgcag cagcgcgcac ggcggcgagc acagttgcgc ggcgcgcaaa gtccgcgtca
     4561 gccatggagg cattgctatg agcgacggct acagcgacgg ctacaaccgg cagccgactg
     4621 tccgcaaaaa gcggcgcgtg accgccgccg aaggcgctcg aatcaccgga ctatccgaac
     4681 gccacgtcgt ccggctcgtg gcgcaggaac gcagcgagtg gctcgccgag caggctgcac
     4741 gccgcgaacg catccgcgcc tatca
//
