LOCUS       sequence-399914-        4765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Complementation of M. smegmatis MceG(E166Q).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert D
  TITLE     Structure of an endogenous mycobacterial MCE lipid transporter.
  JOURNAL   Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi: 
            10.21203/rs.3.rs-2412186/v1. Preprint.
  PUBMED    36711512
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Res Sq. 
            2023 Jan 10:rs.3.rs-2412186. doi: 10.21203/rs.3.rs-2412186/v1. 
            Preprint."
COMMENT     Sequence Label: pBEL2717
FEATURES             Location/Qualifiers
     source          1..4765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2464..2510
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2549..2596
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2615..2989
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYTEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      3041..3629
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3530..3549
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctatgccgag tgccacgcgc gaaacgccga atttccgtgc aacgacgtgt gtcccggacc
       61 gctaccggac agcgaggtcc gcgccatcgc caacagcatt tggcgttgga tcacaaccaa
      121 gtcgcgcatt tgggcggacg ggatcgtggt ctacgaggcc acactcagtg cgcgccagtc
      181 ggccatctcg cggaagggcg cagcagcgcg cacggcggcg agcacagttg cgcggcgcgc
      241 aaagtccgcg tcagccatgg aggcattgct atgagcgacg gctacagcga cggctacaac
      301 cggcagccga ctgtccgcaa aaagcggcgc gtgaccgccg ccgaaggcgc tcgaatcacc
      361 ggactatccg aacgccacgt cgtccggctc gtggcgcagg aacgcagcga gtggctcgcc
      421 gagcaggctg cacgccgcga acgcatccgc gcctatcacg acgacgaggg ccactcttgg
      481 ccgcaaacgg ccaaacattt cgggctgcat ctggacaccg ttaagcgact cggctatcgg
      541 gcgaggaaag agcgtgcggc agaacaggaa gcggctcaaa aggcccacaa cgaagccgac
      601 aatccaccgc tgttctaacg caattgggga gcgggtgtcg cgggggttcc gtggggggtt
      661 ccgttgcaac gggtcggaca ggtaaaagtc ctggtagacg ctagttttct ggtttgggcc
      721 atgcctgtct cgttgcgtgt ttcgttgcgc ccgttttgaa taccagccag acgagacggg
      781 gttctacgaa tcttggtcga taccaagcca tttccgctga atatcgggga gctcaccgcc
      841 agaatcggtg gttgtggtga tgtacgtggc gaactccgtt gtagtgcctg tggtggcatc
      901 cgtggcgcgg ccgcggtacc agatctttaa atctagacgg tgaccacaac gcgcccgctt
      961 tgatcgggga cgtctgcggc cgaccattta cgggtcttgt tgtcgttggc ggtcatgggc
     1021 cgaacatact cacccggatc ggagggccga ggacaaggtc gaacgagggg catgacccgg
     1081 tgcggggctt cttgcactcg gcataggcga gtgctaagaa taacgttggc actcgcgacc
     1141 ggtgagtgct aggtcgggac ggtgaggcca ggcccgtcgt cgcagcgagt ggcagcgagg
     1201 acaacttgag ccgtccgtcg cgggcactgc gcccggccag cgtaagtagc ggggttgccg
     1261 tcacccggtg acccccgttt catccccgat ccggaggaat cacttcgcaa tgggcgtcca
     1321 aatcgacgtg acggggttga gcaagtcttt cgggtcgtca aagatctggg aagacgtgac
     1381 catgtcgatc ccggccggcg aggtgagcgt gctgctgggc ccctccggta ccggcaaatc
     1441 cgtgttcctg aagtcgctga tcggtttgct ccgccccgaa cgtggctcca tcgtcatcga
     1501 cggcaccgac atcctgcagt gctcggccaa ggagctctac gagatccgca cgctgttcgg
     1561 tgtgctgttc caggacggcg cgctgttcgg ctcgatgaac atctacgaca acaccgcgtt
     1621 cccgctccgt gagcacacca agaagagcga gagcgaaatc cgcaagatcg tgatggagaa
     1681 gctcgacctg gtcggtatgc ccaacgacgg gcacaagttc cccggtgaga tctccggcgg
     1741 tatgcgcaag cgcgccggtc tggcccgcgc actcgtgctc gatccggaga tcatcctctg
     1801 cgaccagccg gactcgggtc tggacccggt gcgtacggcc tacctgtcgc agctgctgat
     1861 cgacatcaac gcgcagatcg acgcgaccgt gctgatcgtg acgcacaaca tcaacatcgc
     1921 ccgcaccgtg cccgacaaca tgggcatgct gttccgcaag cagctggtga tgttcggccc
     1981 gcgtgaggtg cttctgacca gcgaggagcc ggtggtcaag cagttcctca acggccgccg
     2041 tatcggcccg atcggtatgt cggaggagaa ggacgaggcc accgcggccg ccgagcaggc
     2101 cctggtggac gccggccagc acgacggcgg tgtcgaggag atcgagggtg tgccgccgca
     2161 gctgcaggcc accccgggta tgcccgagcg caaggccgtg gcccgccgca aggcccgcgt
     2221 gcgcgagatc ctgcacaccc tcccgcccgc cgcgcaggcc gcgatcctcg aagagctcga
     2281 ccgcgaccag ccgcagctgt ccgccccgac cacccagacg gccgccaccg cgcccgtcga
     2341 gaactacgac gactcgccga cgggcgtcat cgaggtgccc aagcaggcgt aagatccagc
     2401 tgcagaattc gaagcttatc gatgtcgacg tagttaacta gcgtacgatc gactgccagg
     2461 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat gccatcatgg
     2521 ccgcggtgat cagctagctc gagcacgtgt tgacaattaa tcatcggcat agtatatcgg
     2581 catagtataa tacgacaagg tgaggaacta aaccatggct aagttgacca gtgccgttcc
     2641 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     2701 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     2761 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     2821 cggcctggac gagctgtaca ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     2881 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     2941 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgag aatctagttc
     3001 catgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3061 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3121 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3181 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3241 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3301 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3361 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3421 tacagcgtga gctatgagaa agcgccacgc ttcccgaggg gagaaaggcg gacaggtatc
     3481 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3541 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3601 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3661 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3721 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3781 gcaacgcgtg agcccaccag ctccgtaagt tcgggcgctg tgtggctcgt acccgcgcat
     3841 tcaggcggca gggggtctaa cgggtctaag gcggcgtgta cggccgccac agcggctctc
     3901 agcggcccgg aaacgtcctc gaaacgacgc atgtgttcct cctggttggt acaggtggtt
     3961 gggggtgctc ggctgtcgct ggtgttccac caccagggct cgacgggaga gcgggggagt
     4021 gtgcagttgt ggggtggccc ctcagcgaaa tatctgactt ggagctcgtg tcggaccata
     4081 caccggtgat taatcgtggt ctactaccaa gcgtgagcca cgtcgccgac gaatttgagc
     4141 agctctggct gccgtactgg ccgctggcaa gcgacgatct gctcgagggg atctaccgcc
     4201 aaagccgcgc gtcggcccta ggccgccggt acatcgaggc gaacccaaca gcgctggcaa
     4261 acctgctggt cgtggacgta gaccatccag acgcagcgct ccgagcgctc agcgcccggg
     4321 ggtcccatcc gctgcccaac gcgatcgtgg gcaatcgcgc caacggccac gcacacgcag
     4381 tgtgggcact caacgcccct gttccacgca ccgaatacgc gcggcgtaag ccgctcgcat
     4441 acatggcggc gtgcgccgaa ggccttcggc gcgccgtcga tggcgaccgc agttactcag
     4501 gcctcatgac caaaaacccc ggccacatcg cctgggaaac ggaatggctc cactcagatc
     4561 tctacacact cagccacatc gaggccgagc tcggcgcgaa catgccaccg ccgcgctggc
     4621 gtcagcagac cacgtacaaa gcggctccga cgccgctagg gcggaattgc gcactgttcg
     4681 attccgtcag gttgtgggcc tatcgtcccg ccctcatgcg gatctacctg ccgacccgga
     4741 acgtggacgg actcggccgc gcgat
//
