LOCUS       sequence-399925-        4765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Complementation of M. smegmatis MceG(K43A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert D
  TITLE     Structure of an endogenous mycobacterial MCE lipid transporter.
  JOURNAL   Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi: 
            10.21203/rs.3.rs-2412186/v1. Preprint.
  PUBMED    36711512
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Res Sq. 
            2023 Jan 10:rs.3.rs-2412186. doi: 10.21203/rs.3.rs-2412186/v1. 
            Preprint."
COMMENT     Sequence Label: pBEL2714
FEATURES             Location/Qualifiers
     source          1..4765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      78..124
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        163..210
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             229..603
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYTEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      655..1243
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1144..1163
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgtaagatc cagctgcaga attcgaagct tatcgatgtc gacgtagtta actagcgtac
       61 gatcgactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      121 ttatgccatc atggccgcgg tgatcagcta gctcgagcac gtgttgacaa ttaatcatcg
      181 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggctaagttg
      241 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
      301 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
      361 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
      421 tgggtgtggg tgcgcggcct ggacgagctg tacaccgagt ggtcggaggt cgtgtccacg
      481 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
      541 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
      601 tgagaatcta gttccatgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
      661 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
      721 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
      781 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
      841 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
      901 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
      961 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1021 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aggggagaaa
     1081 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     1141 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     1201 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     1261 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     1321 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     1381 ccgaacgacc gagcgcaacg cgtgagccca ccagctccgt aagttcgggc gctgtgtggc
     1441 tcgtacccgc gcattcaggc ggcagggggt ctaacgggtc taaggcggcg tgtacggccg
     1501 ccacagcggc tctcagcggc ccggaaacgt cctcgaaacg acgcatgtgt tcctcctggt
     1561 tggtacaggt ggttgggggt gctcggctgt cgctggtgtt ccaccaccag ggctcgacgg
     1621 gagagcgggg gagtgtgcag ttgtggggtg gcccctcagc gaaatatctg acttggagct
     1681 cgtgtcggac catacaccgg tgattaatcg tggtctacta ccaagcgtga gccacgtcgc
     1741 cgacgaattt gagcagctct ggctgccgta ctggccgctg gcaagcgacg atctgctcga
     1801 ggggatctac cgccaaagcc gcgcgtcggc cctaggccgc cggtacatcg aggcgaaccc
     1861 aacagcgctg gcaaacctgc tggtcgtgga cgtagaccat ccagacgcag cgctccgagc
     1921 gctcagcgcc cgggggtccc atccgctgcc caacgcgatc gtgggcaatc gcgccaacgg
     1981 ccacgcacac gcagtgtggg cactcaacgc ccctgttcca cgcaccgaat acgcgcggcg
     2041 taagccgctc gcatacatgg cggcgtgcgc cgaaggcctt cggcgcgccg tcgatggcga
     2101 ccgcagttac tcaggcctca tgaccaaaaa ccccggccac atcgcctggg aaacggaatg
     2161 gctccactca gatctctaca cactcagcca catcgaggcc gagctcggcg cgaacatgcc
     2221 accgccgcgc tggcgtcagc agaccacgta caaagcggct ccgacgccgc tagggcggaa
     2281 ttgcgcactg ttcgattccg tcaggttgtg ggcctatcgt cccgccctca tgcggatcta
     2341 cctgccgacc cggaacgtgg acggactcgg ccgcgcgatc tatgccgagt gccacgcgcg
     2401 aaacgccgaa tttccgtgca acgacgtgtg tcccggaccg ctaccggaca gcgaggtccg
     2461 cgccatcgcc aacagcattt ggcgttggat cacaaccaag tcgcgcattt gggcggacgg
     2521 gatcgtggtc tacgaggcca cactcagtgc gcgccagtcg gccatctcgc ggaagggcgc
     2581 agcagcgcgc acggcggcga gcacagttgc gcggcgcgca aagtccgcgt cagccatgga
     2641 ggcattgcta tgagcgacgg ctacagcgac ggctacaacc ggcagccgac tgtccgcaaa
     2701 aagcggcgcg tgaccgccgc cgaaggcgct cgaatcaccg gactatccga acgccacgtc
     2761 gtccggctcg tggcgcagga acgcagcgag tggctcgccg agcaggctgc acgccgcgaa
     2821 cgcatccgcg cctatcacga cgacgagggc cactcttggc cgcaaacggc caaacatttc
     2881 gggctgcatc tggacaccgt taagcgactc ggctatcggg cgaggaaaga gcgtgcggca
     2941 gaacaggaag cggctcaaaa ggcccacaac gaagccgaca atccaccgct gttctaacgc
     3001 aattggggag cgggtgtcgc gggggttccg tggggggttc cgttgcaacg ggtcggacag
     3061 gtaaaagtcc tggtagacgc tagttttctg gtttgggcca tgcctgtctc gttgcgtgtt
     3121 tcgttgcgcc cgttttgaat accagccaga cgagacgggg ttctacgaat cttggtcgat
     3181 accaagccat ttccgctgaa tatcggggag ctcaccgcca gaatcggtgg ttgtggtgat
     3241 gtacgtggcg aactccgttg tagtgcctgt ggtggcatcc gtggcgcggc cgcggtacca
     3301 gatctttaaa tctagacggt gaccacaacg cgcccgcttt gatcggggac gtctgcggcc
     3361 gaccatttac gggtcttgtt gtcgttggcg gtcatgggcc gaacatactc acccggatcg
     3421 gagggccgag gacaaggtcg aacgaggggc atgacccggt gcggggcttc ttgcactcgg
     3481 cataggcgag tgctaagaat aacgttggca ctcgcgaccg gtgagtgcta ggtcgggacg
     3541 gtgaggccag gcccgtcgtc gcagcgagtg gcagcgagga caacttgagc cgtccgtcgc
     3601 gggcactgcg cccggccagc gtaagtagcg gggttgccgt cacccggtga cccccgtttc
     3661 atccccgatc cggaggaatc acttcgcaat gggcgtccaa atcgacgtga cggggttgag
     3721 caagtctttc gggtcgtcaa agatctggga agacgtgacc atgtcgatcc cggccggcga
     3781 ggtgagcgtg ctgctgggcc cctccggtac cggcgcgtcc gtgttcctga agtcgctgat
     3841 cggtttgctc cgccccgaac gtggctccat cgtcatcgac ggcaccgaca tcctgcagtg
     3901 ctcggccaag gagctctacg agatccgcac gctgttcggt gtgctgttcc aggacggcgc
     3961 gctgttcggc tcgatgaaca tctacgacaa caccgcgttc ccgctccgtg agcacaccaa
     4021 gaagagcgag agcgaaatcc gcaagatcgt gatggagaag ctcgacctgg tcggtatgcc
     4081 caacgacggg cacaagttcc ccggtgagat ctccggcggt atgcgcaagc gcgccggtct
     4141 ggcccgcgca ctcgtgctcg atccggagat catcctctgc gacgagccgg actcgggtct
     4201 ggacccggtg cgtacggcct acctgtcgca gctgctgatc gacatcaacg cgcagatcga
     4261 cgcgaccgtg ctgatcgtga cgcacaacat caacatcgcc cgcaccgtgc ccgacaacat
     4321 gggcatgctg ttccgcaagc agctggtgat gttcggcccg cgtgaggtgc ttctgaccag
     4381 cgaggagccg gtggtcaagc agttcctcaa cggccgccgt atcggcccga tcggtatgtc
     4441 ggaggagaag gacgaggcca ccgcggccgc cgagcaggcc ctggtggacg ccggccagca
     4501 cgacggcggt gtcgaggaga tcgagggtgt gccgccgcag ctgcaggcca ccccgggtat
     4561 gcccgagcgc aaggccgtgg cccgccgcaa ggcccgcgtg cgcgagatcc tgcacaccct
     4621 cccgcccgcc gcgcaggccg cgatcctcga agagctcgac cgcgaccagc cgcagctgtc
     4681 cgccccgacc acccagacgg ccgccaccgc gcccgtcgag aactacgacg actcgccgac
     4741 gggcgtcatc gaggtgccca agcag
//
