LOCUS       sequence-399936-        4447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Complementation of M. smegmatis MceG(∆255-360).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert D
  TITLE     Structure of an endogenous mycobacterial MCE lipid transporter.
  JOURNAL   Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi: 
            10.21203/rs.3.rs-2412186/v1. Preprint.
  PUBMED    36711512
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Res Sq. 
            2023 Jan 10:rs.3.rs-2412186. doi: 10.21203/rs.3.rs-2412186/v1. 
            Preprint."
COMMENT     Sequence Label: pBEL2712
FEATURES             Location/Qualifiers
     source          1..4447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2144..2190
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2229..2276
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2295..2669
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYTEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      2721..3309
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3210..3229
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgccgagtg ccacgcgcga aacgccgaat ttccgtgcaa cgacgtgtgt cccggaccgc
       61 taccggacag cgaggtccgc gccatcgcca acagcatttg gcgttggatc acaaccaagt
      121 cgcgcatttg ggcggacggg atcgtggtct acgaggccac actcagtgcg cgccagtcgg
      181 ccatctcgcg gaagggcgca gcagcgcgca cggcggcgag cacagttgcg cggcgcgcaa
      241 agtccgcgtc agccatggag gcattgctat gagcgacggc tacagcgacg gctacaaccg
      301 gcagccgact gtccgcaaaa agcggcgcgt gaccgccgcc gaaggcgctc gaatcaccgg
      361 actatccgaa cgccacgtcg tccggctcgt ggcgcaggaa cgcagcgagt ggctcgccga
      421 gcaggctgca cgccgcgaac gcatccgcgc ctatcacgac gacgagggcc actcttggcc
      481 gcaaacggcc aaacatttcg ggctgcatct ggacaccgtt aagcgactcg gctatcgggc
      541 gaggaaagag cgtgcggcag aacaggaagc ggctcaaaag gcccacaacg aagccgacaa
      601 tccaccgctg ttctaacgca attggggagc gggtgtcgcg ggggttccgt ggggggttcc
      661 gttgcaacgg gtcggacagg taaaagtcct ggtagacgct agttttctgg tttgggccat
      721 gcctgtctcg ttgcgtgttt cgttgcgccc gttttgaata ccagccagac gagacggggt
      781 tctacgaatc ttggtcgata ccaagccatt tccgctgaat atcggggagc tcaccgccag
      841 aatcggtggt tgtggtgatg tacgtggcga actccgttgt agtgcctgtg gtggcatccg
      901 tggcgcggcc gcggtaccag atctttaaat ctagacggtg accacaacgc gcccgctttg
      961 atcggggacg tctgcggccg accatttacg ggtcttgttg tcgttggcgg tcatgggccg
     1021 aacatactca cccggatcgg agggccgagg acaaggtcga acgaggggca tgacccggtg
     1081 cggggcttct tgcactcggc ataggcgagt gctaagaata acgttggcac tcgcgaccgg
     1141 tgagtgctag gtcgggacgg tgaggccagg cccgtcgtcg cagcgagtgg cagcgaggac
     1201 aacttgagcc gtccgtcgcg ggcactgcgc ccggccagcg taagtagcgg ggttgccgtc
     1261 acccggtgac ccccgtttca tccccgatcc ggaggaatca cttcgcaatg ggcgtccaaa
     1321 tcgacgtgac ggggttgagc aagtctttcg ggtcgtcaaa gatctgggaa gacgtgacca
     1381 tgtcgatccc ggccggcgag gtgagcgtgc tgctgggccc ctccggtacc ggcaaatccg
     1441 tgttcctgaa gtcgctgatc ggtttgctcc gccccgaacg tggctccatc gtcatcgacg
     1501 gcaccgacat cctgcagtgc tcggccaagg agctctacga gatccgcacg ctgttcggtg
     1561 tgctgttcca ggacggcgcg ctgttcggct cgatgaacat ctacgacaac accgcgttcc
     1621 cgctccgtga gcacaccaag aagagcgaga gcgaaatccg caagatcgtg atggagaagc
     1681 tcgacctggt cggtatgccc aacgacgggc acaagttccc cggtgagatc tccggcggta
     1741 tgcgcaagcg cgccggtctg gcccgcgcac tcgtgctcga tccggagatc atcctctgcg
     1801 acgagccgga ctcgggtctg gacccggtgc gtacggccta cctgtcgcag ctgctgatcg
     1861 acatcaacgc gcagatcgac gcgaccgtgc tgatcgtgac gcacaacatc aacatcgccc
     1921 gcaccgtgcc cgacaacatg ggcatgctgt tccgcaagca gctggtgatg ttcggcccgc
     1981 gtgaggtgct tctgaccagc gaggagccgg tggtcaagca gttcctcaac ggccgccgta
     2041 tcggcccgat cggtatgtcg gaggagaagt aagatccagc tgcagaattc gaagcttatc
     2101 gatgtcgacg tagttaacta gcgtacgatc gactgccagg catcaaataa aacgaaaggc
     2161 tcagtcgaaa gactgggcct ttcgttttat gccatcatgg ccgcggtgat cagctagctc
     2221 gagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     2281 tgaggaacta aaccatggct aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     2341 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     2401 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     2461 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtaca
     2521 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     2581 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     2641 tgcacttcgt ggccgaggag caggactgag aatctagttc catgagcgtc agaccccgta
     2701 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2761 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2821 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     2881 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2941 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3001 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3061 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3121 agcgccacgc ttcccgaggg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3181 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3241 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3301 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3361 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3421 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg agcccaccag
     3481 ctccgtaagt tcgggcgctg tgtggctcgt acccgcgcat tcaggcggca gggggtctaa
     3541 cgggtctaag gcggcgtgta cggccgccac agcggctctc agcggcccgg aaacgtcctc
     3601 gaaacgacgc atgtgttcct cctggttggt acaggtggtt gggggtgctc ggctgtcgct
     3661 ggtgttccac caccagggct cgacgggaga gcgggggagt gtgcagttgt ggggtggccc
     3721 ctcagcgaaa tatctgactt ggagctcgtg tcggaccata caccggtgat taatcgtggt
     3781 ctactaccaa gcgtgagcca cgtcgccgac gaatttgagc agctctggct gccgtactgg
     3841 ccgctggcaa gcgacgatct gctcgagggg atctaccgcc aaagccgcgc gtcggcccta
     3901 ggccgccggt acatcgaggc gaacccaaca gcgctggcaa acctgctggt cgtggacgta
     3961 gaccatccag acgcagcgct ccgagcgctc agcgcccggg ggtcccatcc gctgcccaac
     4021 gcgatcgtgg gcaatcgcgc caacggccac gcacacgcag tgtgggcact caacgcccct
     4081 gttccacgca ccgaatacgc gcggcgtaag ccgctcgcat acatggcggc gtgcgccgaa
     4141 ggccttcggc gcgccgtcga tggcgaccgc agttactcag gcctcatgac caaaaacccc
     4201 ggccacatcg cctgggaaac ggaatggctc cactcagatc tctacacact cagccacatc
     4261 gaggccgagc tcggcgcgaa catgccaccg ccgcgctggc gtcagcagac cacgtacaaa
     4321 gcggctccga cgccgctagg gcggaattgc gcactgttcg attccgtcag gttgtgggcc
     4381 tatcgtcccg ccctcatgcg gatctacctg ccgacccgga acgtggacgg actcggccgc
     4441 gcgatct
//
