LOCUS       sequence-400019-        4259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Complementation of M. smegmatis LucB(WT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert D
  TITLE     Structure of an endogenous mycobacterial MCE lipid transporter.
  JOURNAL   Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi: 
            10.21203/rs.3.rs-2412186/v1. Preprint.
  PUBMED    36711512
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Res Sq. 
            2023 Jan 10:rs.3.rs-2412186. doi: 10.21203/rs.3.rs-2412186/v1. 
            Preprint."
COMMENT     Sequence Label: pBEL2836
FEATURES             Location/Qualifiers
     source          1..4259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1502..1548
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        1587..1634
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1653..2027
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYTEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      2079..2667
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2568..2587
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cacgacgacg agggccactc ttggccgcaa acggccaaac atttcgggct gcatctggac
       61 accgttaagc gactcggcta tcgggcgagg aaagagcgtg cggcagaaca ggaagcggct
      121 caaaaggccc acaacgaagc cgacaatcca ccgctgttct aacgcaattg gggagcgggt
      181 gtcgcggggg ttccgtgggg ggttccgttg caacgggtcg gacaggtaaa agtcctggta
      241 gacgctagtt ttctggtttg ggccatgcct gtctcgttgc gtgtttcgtt gcgcccgttt
      301 tgaataccag ccagacgaga cggggttcta cgaatcttgg tcgataccaa gccatttccg
      361 ctgaatatcg gggagctcac cgccagaatc ggtggttgtg gtgatgtacg tggcgaactc
      421 cgttgtagtg cctgtggtgg catccgtggc gcggccgcgg taccagatct ttaaatctag
      481 acggtgacca caacgcgccc gctttgatcg gggacgtctg cggccgacca tttacgggtc
      541 ttgttgtcgt tggcggtcat gggccgaaca tactcacccg gatcggaggg ccgaggacaa
      601 ggtcgaacga ggggcatgac ccggtgcggg gcttcttgca ctcggcatag gcgagtgcta
      661 agaataacgt tggcactcgc gaccggtgag tgctaggtcg ggacggtgag gccaggcccg
      721 tcgtcgcagc gagtggcagc gaggacaact tgagccgtcc gtcgcgggca ctgcgcccgg
      781 ccagcgtaag tagcggggtt gccgtcaccc ggtgaccccc gtttcatccc cgatccggag
      841 gaatcacttc gcaatgagca agtggttact gcgcggagtg gtgttcgcaa cggcgatggt
      901 gatcgtgcgc ctgctacagg gcgcactggt caacgcgtcc ccgggcaacg ccatctggtt
      961 cagcacggga ctgctggtcc tgtacgcgat cggcgtcgcg gtctggggcg tcctcgacgg
     1021 tcgcggcgac gcgcgcagca accccgatcc ggatcgccgc gccgacctgg cgatgacgtg
     1081 gcttctggcc ggcctggccg cgggcatcct cagcggcgcg gtgtcgtggt tcatcgggct
     1141 gttctacaag agcatctaca ccgagtcgct gctgaacgag atcaccacct tcgccgcctt
     1201 cacggcgctg ctgacgttcc tggtggccgt cgcgggcgtg accatcggcc gctggacgat
     1261 cgaccgcaag gccccgccgg tcacgcgcac ccgccacgga ctggccgccg atgacgaccg
     1321 cgccgacacc gacgtgttcg ccgcggtgag cgccaacggg gcgcaggagc acaccgacac
     1381 gacgcagacc acaccgctgg agaatccgga ccagccgcgt cagagctgag gatccagctg
     1441 cagaattcga agcttatcga tgtcgacgta gttaactagc gtacgatcga ctgccaggca
     1501 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatgc catcatggcc
     1561 gcggtgatca gctagctcga gcacgtgttg acaattaatc atcggcatag tatatcggca
     1621 tagtataata cgacaaggtg aggaactaaa ccatggctaa gttgaccagt gccgttccgg
     1681 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     1741 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     1801 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     1861 gcctggacga gctgtacacc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     1921 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     1981 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgagaa tctagttcca
     2041 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2101 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2161 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2221 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2281 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2341 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2401 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2461 cagcgtgagc tatgagaaag cgccacgctt cccgagggga gaaaggcgga caggtatccg
     2521 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2581 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2641 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2701 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     2761 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     2821 aacgcgtgag cccaccagct ccgtaagttc gggcgctgtg tggctcgtac ccgcgcattc
     2881 aggcggcagg gggtctaacg ggtctaaggc ggcgtgtacg gccgccacag cggctctcag
     2941 cggcccggaa acgtcctcga aacgacgcat gtgttcctcc tggttggtac aggtggttgg
     3001 gggtgctcgg ctgtcgctgg tgttccacca ccagggctcg acgggagagc gggggagtgt
     3061 gcagttgtgg ggtggcccct cagcgaaata tctgacttgg agctcgtgtc ggaccataca
     3121 ccggtgatta atcgtggtct actaccaagc gtgagccacg tcgccgacga atttgagcag
     3181 ctctggctgc cgtactggcc gctggcaagc gacgatctgc tcgaggggat ctaccgccaa
     3241 agccgcgcgt cggccctagg ccgccggtac atcgaggcga acccaacagc gctggcaaac
     3301 ctgctggtcg tggacgtaga ccatccagac gcagcgctcc gagcgctcag cgcccggggg
     3361 tcccatccgc tgcccaacgc gatcgtgggc aatcgcgcca acggccacgc acacgcagtg
     3421 tgggcactca acgcccctgt tccacgcacc gaatacgcgc ggcgtaagcc gctcgcatac
     3481 atggcggcgt gcgccgaagg ccttcggcgc gccgtcgatg gcgaccgcag ttactcaggc
     3541 ctcatgacca aaaaccccgg ccacatcgcc tgggaaacgg aatggctcca ctcagatctc
     3601 tacacactca gccacatcga ggccgagctc ggcgcgaaca tgccaccgcc gcgctggcgt
     3661 cagcagacca cgtacaaagc ggctccgacg ccgctagggc ggaattgcgc actgttcgat
     3721 tccgtcaggt tgtgggccta tcgtcccgcc ctcatgcgga tctacctgcc gacccggaac
     3781 gtggacggac tcggccgcgc gatctatgcc gagtgccacg cgcgaaacgc cgaatttccg
     3841 tgcaacgacg tgtgtcccgg accgctaccg gacagcgagg tccgcgccat cgccaacagc
     3901 atttggcgtt ggatcacaac caagtcgcgc atttgggcgg acgggatcgt ggtctacgag
     3961 gccacactca gtgcgcgcca gtcggccatc tcgcggaagg gcgcagcagc gcgcacggcg
     4021 gcgagcacag ttgcgcggcg cgcaaagtcc gcgtcagcca tggaggcatt gctatgagcg
     4081 acggctacag cgacggctac aaccggcagc cgactgtccg caaaaagcgg cgcgtgaccg
     4141 ccgccgaagg cgctcgaatc accggactat ccgaacgcca cgtcgtccgg ctcgtggcgc
     4201 aggaacgcag cgagtggctc gccgagcagg ctgcacgccg cgaacgcatc cgcgcctat
//
