LOCUS       sequence-400021-        4618 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Complementation of M. smegmatis MceG(∆312-360).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert D
  TITLE     Structure of an endogenous mycobacterial MCE lipid transporter.
  JOURNAL   Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi: 
            10.21203/rs.3.rs-2412186/v1. Preprint.
  PUBMED    36711512
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Res Sq. 
            2023 Jan 10:rs.3.rs-2412186. doi: 10.21203/rs.3.rs-2412186/v1. 
            Preprint."
COMMENT     Sequence Label: pBEL2709
FEATURES             Location/Qualifiers
     source          1..4618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1857..1903
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        1942..1989
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2008..2382
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYTEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      2434..3022
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2923..2942
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgacgaggg ccactcttgg ccgcaaacgg ccaaacattt cgggctgcat ctggacaccg
       61 ttaagcgact cggctatcgg gcgaggaaag agcgtgcggc agaacaggaa gcggctcaaa
      121 aggcccacaa cgaagccgac aatccaccgc tgttctaacg caattgggga gcgggtgtcg
      181 cgggggttcc gtggggggtt ccgttgcaac gggtcggaca ggtaaaagtc ctggtagacg
      241 ctagttttct ggtttgggcc atgcctgtct cgttgcgtgt ttcgttgcgc ccgttttgaa
      301 taccagccag acgagacggg gttctacgaa tcttggtcga taccaagcca tttccgctga
      361 atatcgggga gctcaccgcc agaatcggtg gttgtggtga tgtacgtggc gaactccgtt
      421 gtagtgcctg tggtggcatc cgtggcgcgg ccgcggtacc agatctttaa atctagacgg
      481 tgaccacaac gcgcccgctt tgatcgggga cgtctgcggc cgaccattta cgggtcttgt
      541 tgtcgttggc ggtcatgggc cgaacatact cacccggatc ggagggccga ggacaaggtc
      601 gaacgagggg catgacccgg tgcggggctt cttgcactcg gcataggcga gtgctaagaa
      661 taacgttggc actcgcgacc ggtgagtgct aggtcgggac ggtgaggcca ggcccgtcgt
      721 cgcagcgagt ggcagcgagg acaacttgag ccgtccgtcg cgggcactgc gcccggccag
      781 cgtaagtagc ggggttgccg tcacccggtg acccccgttt catccccgat ccggaggaat
      841 cacttcgcaa tgggcgtcca aatcgacgtg acggggttga gcaagtcttt cgggtcgtca
      901 aagatctggg aagacgtgac catgtcgatc ccggccggcg aggtgagcgt gctgctgggc
      961 ccctccggta ccggcaaatc cgtgttcctg aagtcgctga tcggtttgct ccgccccgaa
     1021 cgtggctcca tcgtcatcga cggcaccgac atcctgcagt gctcggccaa ggagctctac
     1081 gagatccgca cgctgttcgg tgtgctgttc caggacggcg cgctgttcgg ctcgatgaac
     1141 atctacgaca acaccgcgtt cccgctccgt gagcacacca agaagagcga gagcgaaatc
     1201 cgcaagatcg tgatggagaa gctcgacctg gtcggtatgc ccaacgacgg gcacaagttc
     1261 cccggtgaga tctccggcgg tatgcgcaag cgcgccggtc tggcccgcgc actcgtgctc
     1321 gatccggaga tcatcctctg cgacgagccg gactcgggtc tggacccggt gcgtacggcc
     1381 tacctgtcgc agctgctgat cgacatcaac gcgcagatcg acgcgaccgt gctgatcgtg
     1441 acgcacaaca tcaacatcgc ccgcaccgtg cccgacaaca tgggcatgct gttccgcaag
     1501 cagctggtga tgttcggccc gcgtgaggtg cttctgacca gcgaggagcc ggtggtcaag
     1561 cagttcctca acggccgccg tatcggcccg atcggtatgt cggaggagaa ggacgaggcc
     1621 accgcggccg ccgagcaggc cctggtggac gccggccagc acgacggcgg tgtcgaggag
     1681 atcgagggtg tgccgccgca gctgcaggcc accccgggta tgcccgagcg caaggccgtg
     1741 gcccgccgca aggcccgcgt gcgcgagatc ctgcacaccc tctaagatcc agctgcagaa
     1801 ttcgaagctt atcgatgtcg acgtagttaa ctagcgtacg atcgactgcc aggcatcaaa
     1861 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatgccatca tggccgcggt
     1921 gatcagctag ctcgagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     1981 taatacgaca aggtgaggaa ctaaaccatg gctaagttga ccagtgccgt tccggtgctc
     2041 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     2101 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     2161 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     2221 gacgagctgt acaccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     2281 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     2341 gccggcaact gcgtgcactt cgtggccgag gagcaggact gagaatctag ttccatgagc
     2401 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2461 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2521 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2581 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2641 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2701 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2761 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2821 tgagctatga gaaagcgcca cgcttcccga ggggagaaag gcggacaggt atccggtaag
     2881 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2941 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3001 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3061 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3121 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcaacgc
     3181 gtgagcccac cagctccgta agttcgggcg ctgtgtggct cgtacccgcg cattcaggcg
     3241 gcagggggtc taacgggtct aaggcggcgt gtacggccgc cacagcggct ctcagcggcc
     3301 cggaaacgtc ctcgaaacga cgcatgtgtt cctcctggtt ggtacaggtg gttgggggtg
     3361 ctcggctgtc gctggtgttc caccaccagg gctcgacggg agagcggggg agtgtgcagt
     3421 tgtggggtgg cccctcagcg aaatatctga cttggagctc gtgtcggacc atacaccggt
     3481 gattaatcgt ggtctactac caagcgtgag ccacgtcgcc gacgaatttg agcagctctg
     3541 gctgccgtac tggccgctgg caagcgacga tctgctcgag gggatctacc gccaaagccg
     3601 cgcgtcggcc ctaggccgcc ggtacatcga ggcgaaccca acagcgctgg caaacctgct
     3661 ggtcgtggac gtagaccatc cagacgcagc gctccgagcg ctcagcgccc gggggtccca
     3721 tccgctgccc aacgcgatcg tgggcaatcg cgccaacggc cacgcacacg cagtgtgggc
     3781 actcaacgcc cctgttccac gcaccgaata cgcgcggcgt aagccgctcg catacatggc
     3841 ggcgtgcgcc gaaggccttc ggcgcgccgt cgatggcgac cgcagttact caggcctcat
     3901 gaccaaaaac cccggccaca tcgcctggga aacggaatgg ctccactcag atctctacac
     3961 actcagccac atcgaggccg agctcggcgc gaacatgcca ccgccgcgct ggcgtcagca
     4021 gaccacgtac aaagcggctc cgacgccgct agggcggaat tgcgcactgt tcgattccgt
     4081 caggttgtgg gcctatcgtc ccgccctcat gcggatctac ctgccgaccc ggaacgtgga
     4141 cggactcggc cgcgcgatct atgccgagtg ccacgcgcga aacgccgaat ttccgtgcaa
     4201 cgacgtgtgt cccggaccgc taccggacag cgaggtccgc gccatcgcca acagcatttg
     4261 gcgttggatc acaaccaagt cgcgcatttg ggcggacggg atcgtggtct acgaggccac
     4321 actcagtgcg cgccagtcgg ccatctcgcg gaagggcgca gcagcgcgca cggcggcgag
     4381 cacagttgcg cggcgcgcaa agtccgcgtc agccatggag gcattgctat gagcgacggc
     4441 tacagcgacg gctacaaccg gcagccgact gtccgcaaaa agcggcgcgt gaccgccgcc
     4501 gaaggcgctc gaatcaccgg actatccgaa cgccacgtcg tccggctcgt ggcgcaggaa
     4561 cgcagcgagt ggctcgccga gcaggctgca cgccgcgaac gcatccgcgc ctatcacg
//
