LOCUS       sequence-400022-        4564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Complementation of M. smegmatis MceG(∆294-360).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert D
  TITLE     Structure of an endogenous mycobacterial MCE lipid transporter.
  JOURNAL   Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi: 
            10.21203/rs.3.rs-2412186/v1. Preprint.
  PUBMED    36711512
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Res Sq. 
            2023 Jan 10:rs.3.rs-2412186. doi: 10.21203/rs.3.rs-2412186/v1. 
            Preprint."
COMMENT     Sequence Label: pBEL2710
FEATURES             Location/Qualifiers
     source          1..4564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2260..2306
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2345..2392
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2411..2785
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYTEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      2837..3425
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3326..3345
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgccgagtgc cacgcgcgaa acgccgaatt tccgtgcaac gacgtgtgtc ccggaccgct
       61 accggacagc gaggtccgcg ccatcgccaa cagcatttgg cgttggatca caaccaagtc
      121 gcgcatttgg gcggacggga tcgtggtcta cgaggccaca ctcagtgcgc gccagtcggc
      181 catctcgcgg aagggcgcag cagcgcgcac ggcggcgagc acagttgcgc ggcgcgcaaa
      241 gtccgcgtca gccatggagg cattgctatg agcgacggct acagcgacgg ctacaaccgg
      301 cagccgactg tccgcaaaaa gcggcgcgtg accgccgccg aaggcgctcg aatcaccgga
      361 ctatccgaac gccacgtcgt ccggctcgtg gcgcaggaac gcagcgagtg gctcgccgag
      421 caggctgcac gccgcgaacg catccgcgcc tatcacgacg acgagggcca ctcttggccg
      481 caaacggcca aacatttcgg gctgcatctg gacaccgtta agcgactcgg ctatcgggcg
      541 aggaaagagc gtgcggcaga acaggaagcg gctcaaaagg cccacaacga agccgacaat
      601 ccaccgctgt tctaacgcaa ttggggagcg ggtgtcgcgg gggttccgtg gggggttccg
      661 ttgcaacggg tcggacaggt aaaagtcctg gtagacgcta gttttctggt ttgggccatg
      721 cctgtctcgt tgcgtgtttc gttgcgcccg ttttgaatac cagccagacg agacggggtt
      781 ctacgaatct tggtcgatac caagccattt ccgctgaata tcggggagct caccgccaga
      841 atcggtggtt gtggtgatgt acgtggcgaa ctccgttgta gtgcctgtgg tggcatccgt
      901 ggcgcggccg cggtaccaga tctttaaatc tagacggtga ccacaacgcg cccgctttga
      961 tcggggacgt ctgcggccga ccatttacgg gtcttgttgt cgttggcggt catgggccga
     1021 acatactcac ccggatcgga gggccgagga caaggtcgaa cgaggggcat gacccggtgc
     1081 ggggcttctt gcactcggca taggcgagtg ctaagaataa cgttggcact cgcgaccggt
     1141 gagtgctagg tcgggacggt gaggccaggc ccgtcgtcgc agcgagtggc agcgaggaca
     1201 acttgagccg tccgtcgcgg gcactgcgcc cggccagcgt aagtagcggg gttgccgtca
     1261 cccggtgacc cccgtttcat ccccgatccg gaggaatcac ttcgcaatgg gcgtccaaat
     1321 cgacgtgacg gggttgagca agtctttcgg gtcgtcaaag atctgggaag acgtgaccat
     1381 gtcgatcccg gccggcgagg tgagcgtgct gctgggcccc tccggtaccg gcaaatccgt
     1441 gttcctgaag tcgctgatcg gtttgctccg ccccgaacgt ggctccatcg tcatcgacgg
     1501 caccgacatc ctgcagtgct cggccaagga gctctacgag atccgcacgc tgttcggtgt
     1561 gctgttccag gacggcgcgc tgttcggctc gatgaacatc tacgacaaca ccgcgttccc
     1621 gctccgtgag cacaccaaga agagcgagag cgaaatccgc aagatcgtga tggagaagct
     1681 cgacctggtc ggtatgccca acgacgggca caagttcccc ggtgagatct ccggcggtat
     1741 gcgcaagcgc gccggtctgg cccgcgcact cgtgctcgat ccggagatca tcctctgcga
     1801 cgagccggac tcgggtctgg acccggtgcg tacggcctac ctgtcgcagc tgctgatcga
     1861 catcaacgcg cagatcgacg cgaccgtgct gatcgtgacg cacaacatca acatcgcccg
     1921 caccgtgccc gacaacatgg gcatgctgtt ccgcaagcag ctggtgatgt tcggcccgcg
     1981 tgaggtgctt ctgaccagcg aggagccggt ggtcaagcag ttcctcaacg gccgccgtat
     2041 cggcccgatc ggtatgtcgg aggagaagga cgaggccacc gcggccgccg agcaggccct
     2101 ggtggacgcc ggccagcacg acggcggtgt cgaggagatc gagggtgtgc cgccgcagct
     2161 gcaggccacc ccgggtatgc ccgagtaaga tccagctgca gaattcgaag cttatcgatg
     2221 tcgacgtagt taactagcgt acgatcgact gccaggcatc aaataaaacg aaaggctcag
     2281 tcgaaagact gggcctttcg ttttatgcca tcatggccgc ggtgatcagc tagctcgagc
     2341 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     2401 gaactaaacc atggctaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     2461 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     2521 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     2581 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacaccga
     2641 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     2701 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     2761 cttcgtggcc gaggagcagg actgagaatc tagttccatg agcgtcagac cccgtagaaa
     2821 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2881 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2941 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     3001 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3061 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3121 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3181 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3241 ccacgcttcc cgaggggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3301 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3361 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3421 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3481 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     3541 gagctgatac cgctcgccgc agccgaacga ccgagcgcaa cgcgtgagcc caccagctcc
     3601 gtaagttcgg gcgctgtgtg gctcgtaccc gcgcattcag gcggcagggg gtctaacggg
     3661 tctaaggcgg cgtgtacggc cgccacagcg gctctcagcg gcccggaaac gtcctcgaaa
     3721 cgacgcatgt gttcctcctg gttggtacag gtggttgggg gtgctcggct gtcgctggtg
     3781 ttccaccacc agggctcgac gggagagcgg gggagtgtgc agttgtgggg tggcccctca
     3841 gcgaaatatc tgacttggag ctcgtgtcgg accatacacc ggtgattaat cgtggtctac
     3901 taccaagcgt gagccacgtc gccgacgaat ttgagcagct ctggctgccg tactggccgc
     3961 tggcaagcga cgatctgctc gaggggatct accgccaaag ccgcgcgtcg gccctaggcc
     4021 gccggtacat cgaggcgaac ccaacagcgc tggcaaacct gctggtcgtg gacgtagacc
     4081 atccagacgc agcgctccga gcgctcagcg cccgggggtc ccatccgctg cccaacgcga
     4141 tcgtgggcaa tcgcgccaac ggccacgcac acgcagtgtg ggcactcaac gcccctgttc
     4201 cacgcaccga atacgcgcgg cgtaagccgc tcgcatacat ggcggcgtgc gccgaaggcc
     4261 ttcggcgcgc cgtcgatggc gaccgcagtt actcaggcct catgaccaaa aaccccggcc
     4321 acatcgcctg ggaaacggaa tggctccact cagatctcta cacactcagc cacatcgagg
     4381 ccgagctcgg cgcgaacatg ccaccgccgc gctggcgtca gcagaccacg tacaaagcgg
     4441 ctccgacgcc gctagggcgg aattgcgcac tgttcgattc cgtcaggttg tgggcctatc
     4501 gtcccgccct catgcggatc tacctgccga cccggaacgt ggacggactc ggccgcgcga
     4561 tcta
//
