LOCUS       sequence-400058-        9267 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3 input AND circuit on pLW555 backbone: PTet-P1, PLux-A1, 
            PIcaRA:PPhlF:PAmtR-Q2, PTac-I1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9267)
  AUTHORS   Lebovich M, Zeng M, Andrews LB
  TITLE     Algorithmic Programming of Sequential Logic and Genetic Circuits for
            Recording Biochemical Concentration in a Probiotic Bacterium.
  JOURNAL   ACS Synth Biol. 2023 Aug 15. doi: 10.1021/acssynbio.3c00232.
  PUBMED    37581922
REFERENCE   2  (bases 1 to 9267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Aug 15. doi: 10.1021/acssynbio.3c00232."
COMMENT     Sequence Label: pML31
FEATURES             Location/Qualifiers
     source          1..9267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(399..1214)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(399..1214)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1122..1141
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1206..1225
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1396..1414
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1421..1439
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        join(4123..4128,4129..4144,4145..4151)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    4157..4176
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        4926..4960
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     RBS             4969..4980
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5836..5847
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5965..7047
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(5984..6003)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             7066..7689
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(7139..7158)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(8648..8667)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      complement(join(9032..9267,1..310))
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(9112..9131)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag
       61 tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga
      121 ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga
      181 ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga
      241 aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa
      301 acgatctcaa gaagatcatc ttattaaggg gtctgacgct cagtggaacg aaaaatcaat
      361 ctaaagtata tatgagtaaa cttggtctga cagttacctt agaaaaactc atcgagcatc
      421 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
      481 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
      541 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
      601 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
      661 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
      721 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
      781 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
      841 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
      901 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
      961 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     1021 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     1081 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     1141 ccatataaat cagcatccat gttggaattt aatcgcggcc tggagcaaga cgtttcccgt
     1201 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     1261 catgatgata tatttttatc ttgtgcaatg tacatcagag attttgagac acaaccaatt
     1321 attgaaggcc tccctaacgg ggggcctttt tttgtttctg gtctcccgct tggagtactc
     1381 caccgttggc ttttttccct atcagtgata gagattgaca tccctatcag tgatagagat
     1441 aatgagcacc tgagcggtca acgcatgtgc tttgcgttct gatgagacag tgatgtcgaa
     1501 accgcctcta caaataattt tgtttaacta tggactatgt ttgaaaggga gaaatactag
     1561 atggcacgta ccccgagccg tagcagcatt ggtagcctgc gtagtccgca tacccataaa
     1621 gcaattctga ccagcaccat tgaaatcctg aaagaatgtg gttatagcgg tctgagcatt
     1681 gaaagcgttg cacgtcgtgc cggtgcaagc aaaccgacca tttatcgttg gtggaccaat
     1741 aaagcagcac tgattgccga agtgtatgaa aatgaaagcg aacaggtgcg taaatttccg
     1801 gatctgggta gctttaaagc cgatctggat tttctgctgc gtaatctgtg gaaagtttgg
     1861 cgtgaaacca tttgtggtga agcatttcgt tgtgttattg cagaagcaca gctggaccct
     1921 gcaaccctga cccagctgaa agatcagttt atggaacgtc gtcgtgagat gccgaaaaaa
     1981 ctggttgaaa atgccattag caatggtgaa ctgccgaaag ataccaatcg tgaactgctg
     2041 ctggatatga tttttggttt ttgttggtat cgcctgctga ccgaacagct gaccgttgaa
     2101 caggatattg aagaatttac cttcctgctg attaatggtg tttgtccggg tacacagcgt
     2161 taaggaaaca cagaaaaaag cccgcacctg acagtgcggg cttttttttt cgaccaaagg
     2221 aggtcgctgg agatagcttc ttaccggacc tgtaggatcg tacaggttta cgcaagaaaa
     2281 tggtttgtta ctttcgaata aactgagggt gtctcaaggt gcgtaccttg actgatgagt
     2341 ccgaaaggac gaaacacccc tctacaaata attttgttta aaatgttccc taataatcag
     2401 caaagaggtt actagatggc aggcgcagtt ggtcgtccgc gtcgtagtgc accgcgtcgt
     2461 gcaggtaaaa atccgcgtga agaaattctg gatgcaagcg cagaactgtt tacccgtcag
     2521 ggttttgcaa ccaccagtac ccatcagatt gcagatgcag ttggtattcg tcaggcaagc
     2581 ctgtattatc attttccgag caaaaccgaa atctttctga ccctgctgaa aagcaccgtt
     2641 gaaccgagca ccgttctggc agaagatctg agcaccctgg atgcaggtcc ggaaatgcgt
     2701 ctgtgggcaa ttgttgcaag cgaagttcgt ctgctgctga gcaccaaatg gaatgttggt
     2761 cgtctgtatc agctgccgat tgttggtagc gaagaatttg cagaatatca tagccagcgt
     2821 gaagcactga ccaatgtttt tcgtgatctg gcaaccgaaa ttgttggtga tgatccgcgt
     2881 gcagaactgc cgtttcatat taccatgagc gttattgaaa tgcgtcgcaa tgatggtaaa
     2941 attccgagtc cgctgagcgc agatagcctg ccggaaaccg caattatgct ggcagatgca
     3001 agcctggcag ttctgggtgc accgctgcct gcagatcgtg ttgaaaaaac cctggaactg
     3061 attaaacagg cagatgcaaa ataactcggt accaaagacg aacaataaga cgctgaaaag
     3121 cgtctttttt cgttttggtc caggttactg gagcgacgta cggtggaatc tgattcgtta
     3181 ccaattgaca tgatacgaaa cgtaccgtat cgttaaggtt acggtcaact cataagattc
     3241 tgattcgtta ccaattgaca attcacctac ctttcgttag gttaggttgt aatgcttgtc
     3301 caaccaaatg attcgttacc aattgacagt ttctatcgat ctatagataa tgctagcctg
     3361 agtcgtcaag tgctgtgctt gcacttctga tgaggcagtg atgccgaaac gacctctaca
     3421 aataattttg tttaagccat gccattggct tttgatagag gacaactact agatgaacct
     3481 gaaagataaa attctgggcg ttgccaaaga actgtttatc aaaaatggct ataacgcaac
     3541 caccaccggt gaaattgtta aactgagcga aagcagcaaa ggcaatctgt attatcactt
     3601 taaaaccaaa gagaacctgt ttctggaaat cctgaacatc gaagaaagca aatggcaaga
     3661 gcagtggaaa aaagaacaaa tcaaatgcaa aaccaaccgc gagaaattct atctgtataa
     3721 tgaactgagc ctgaccaccg aatattacta tccgctgcag aatgccatca tcgagtttta
     3781 taccgagtac tataaaacca acagcatcaa cgagaaaatg aacaaactgg aaaacaaata
     3841 catcgatgcc taccacgtga tctttaaaga aggtaatctg aacggcgaat ggtgcattaa
     3901 tgatgttaat gccgtgagca aaattgcagc aaatgccgtt aatggcattg ttacctttac
     3961 ccatgagcag aatatcaacg aacgcattaa actgatgaac aaattcagcc agatctttct
     4021 gaatggcctg agcaaataag tcagtttcac ctgttttacg taaaaacccg cttcggcggg
     4081 tttttacttt tggaggttgc cggagaacga tcgttggctg tgttgacaat taatcatcgg
     4141 ctcgtataat gtgtggaatt gtgagcgctc acaattctga gccccatagg gtggtgtgta
     4201 ccacccctga tgagtccaaa aggacgaaat ggggcctcta caaataattt tgtttaaatt
     4261 gctatggact atgtttcaaa gtgagaatac taggtgaaag acaaaattat cgataacgcc
     4321 atcaccctgt ttagcgaaaa aggttatgac ggcaccaccc tggatgatat tgcaaaaagc
     4381 gtgaacatca aaaaagccag cctgtattat cactttgata gcaaaaaaag catctacgag
     4441 cagagcgtta aatgctgttt cgattatctg aacaacatca tcatgatgaa ccagaacaaa
     4501 agcaactata gcatcgatgc cctgtatcag tttctgtttg agttcatctt cgatatcgag
     4561 gaacgctata ttcgtatgta tgttcagctg agcaacacac cggaagaatt ttcaggtaac
     4621 atttatggcc agatccagga tctgaatcag agcctgagca aagaaatcgc caaattctat
     4681 gacgaaagca aaatcaaaat gaccaaagag gacttccaga atctgattct gctgtttctg
     4741 gaaagctggt atctgaaagc cagctttagc cagaaatttg gtgcagttga agaaagcaaa
     4801 agccagttta aagatgaggt ttatagcctg ctgaacatct ttctgaagaa ataaacaatt
     4861 ttcgaaaaaa cccgcttcgg cgggtttttt tatagctaaa aaggtaatgg tcaccatata
     4921 tcaagtttac ggctagctca gtcctaggta ctatgctagc tactagagaa agaggagaaa
     4981 tactagatgg ctgaagcgca aaatgatccc ctgctgccgg gatactcgtt taatgcccat
     5041 ctggtggcgg gtttaacgcc gattgaggcc aacggttatc tcgatttttt tatcgaccga
     5101 ccgctgggaa tgaaaggtta tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa
     5161 aatcagggac gagaatttgt ttgccgaccg ggtgatattt tgctgttccc gccaggagag
     5221 attcatcact acggtcgtca tccggaggct cgcgaatggt atcaccagtg ggtttacttt
     5281 cgtccgcgcg cctactggca tgaatggctt aactggccgt caatatttgc caatacgggg
     5341 ttctttcgcc cggatgaagc gcaccagccg catttcagcg acctgtttgg gcaaatcatt
     5401 aacgccgggc aaggggaagg gcgctattcg gagctgctgg cgataaatct gcttgagcaa
     5461 ttgttactgc ggcgcatgga agcgattaac gagtcgctcc atccaccgat ggataatcgg
     5521 gtacgcgagg cttgtcagta catcagcgat cacctggcag acagcaattt tgatatcgcc
     5581 agcgtcgcac agcatgtttg cttgtcgccg tcgcgtctgt cacatctttt ccgccagcag
     5641 ttagggatta gcgtcttaag ctggcgcgag gaccaacgta tcagccaggc gaagctgctt
     5701 ttgagcacca cccggatgcc tatcgccacc gtcggtcgca atgttggttt tgacgatcaa
     5761 ctctatttct cgcgggtatt taaaaaatgc accggggcca gcccgagcga gttccgtgcc
     5821 ggttaataac caattattga aggccgctaa cgcagccttt ttttgtttct ggtctcccaa
     5881 tggcggcgcg ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa
     5941 gagagtcaat tcagggtggt gaatatgaaa ccagtaacgt tatacgatgt cgcagagtat
     6001 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
     6061 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
     6121 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
     6181 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
     6241 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
     6301 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
     6361 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac
     6421 cagacaccca tcaacagtat tattttctcc catgaggacg gtacgcgact gggcgtggag
     6481 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
     6541 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg
     6601 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
     6661 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
     6721 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga
     6781 tacgacgata ccgaagatag ctcatgttat atcccgccgt taaccaccat caaacaggat
     6841 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
     6901 gtgaagggca atcagctgtt gccagtctca ctggtgaaaa gaaaaaccac cctggcgccc
     6961 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     7021 gtttcccgac tggaaagcgg gcagtgacaa tccaggagga aaaaaatgtc cagattagat
     7081 aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta
     7141 acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta
     7201 aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact
     7261 cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt
     7321 tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct
     7381 acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt
     7441 tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta
     7501 ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt
     7561 atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc
     7621 ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt
     7681 gggtcctaaa tcacacagga aaggcctcga tgaaaaacat aaatgccgac gacacataca
     7741 gaataattaa taaaattaaa gcttgtagaa gcaataatga tattaatcaa tgcttatctg
     7801 atatgactaa aatggtacat tgtgaatatt atttactcgc gatcatttat cctcattcta
     7861 tggttaaatc tgatatttca atcctagata attaccctaa aaaatggagg caatattatg
     7921 atgacgctaa tttaataaaa tatgatccta tagtagatta ttctaactcc aatcattcac
     7981 caattaattg gaatatattt gaaaacaatg ctgtaaataa aaaatctcca aatgtaatta
     8041 aagaagcgaa aacatcaggt cttatcactg ggtttagttt ccctattcat acggctaaca
     8101 atggcttcgg aatgcttagt tttgcacatt cagaaaaaga caactatata gatagtttat
     8161 ttttacatgc gtgtatgaac ataccattaa ttgttccttc tctagttgat aattatcgaa
     8221 aaataaatat agcaaataat aaatcaaaca acgatttaac caaaagagaa aaagaatgtt
     8281 tagcgtgggc atgcgaagga aaaagctctt gggatatttc aaaaatatta ggttgcagtg
     8341 agcgtactgt cactttccat ttaaccaatg cgcaaatgaa actcaataca acaaaccgct
     8401 gccaaagtat ttctaaagca attttaacag gagcaattga ttgcccatac tttaaaaatt
     8461 aaccataatt ggtaacgaat cagacaattg acggctcgag ggagtagcat agggtttgca
     8521 gaatccctgc ttcgtccatt tgacaggcac attatgcatc gatgataagc tgtcaaacat
     8581 gagcagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     8641 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     8701 atgagcaaat attttatctg aggtgcttcc tcgctcactg actcgctgca cgaggcagac
     8761 ctcagcgcta gcggagtgta tactggctta ctatgttggc actgatgagg gtgtcagtga
     8821 agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac
     8881 aggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg actgcggcga
     8941 gcggaaatgg cttacgaacg gggcggagat ttcccggaag atgccaggaa gatacttaac
     9001 agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc cctgacaagc
     9061 atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc
     9121 aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
     9181 gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg
     9241 taggcagttc gctccaagct ggactgt
//
