LOCUS       sequence-400061-        9590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2-bit register circuit on pLW555 backbone: PTac-S2, PTet-P2, 
            PLux-A1, PLux-H1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   Lebovich M, Zeng M, Andrews LB
  TITLE     Algorithmic Programming of Sequential Logic and Genetic Circuits for
            Recording Biochemical Concentration in a Probiotic Bacterium.
  JOURNAL   ACS Synth Biol. 2023 Aug 15. doi: 10.1021/acssynbio.3c00232.
  PUBMED    37581922
REFERENCE   2  (bases 1 to 9590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Aug 15. doi: 10.1021/acssynbio.3c00232."
COMMENT     Sequence Label: pML32
FEATURES             Location/Qualifiers
     source          1..9590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(399..1214)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(399..1214)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1122..1141
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1206..1225
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        join(1393..1398,1399..1414,1415..1421)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1427..1446
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    2461..2479
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2486..2504
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        5249..5283
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     RBS             5292..5303
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             6159..6170
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             6288..7370
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6307..6326)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             7389..8012
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(7462..7481)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(8971..8990)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      complement(join(9355..9590,1..310))
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(9435..9454)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag
       61 tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga
      121 ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga
      181 ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga
      241 aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa
      301 acgatctcaa gaagatcatc ttattaaggg gtctgacgct cagtggaacg aaaaatcaat
      361 ctaaagtata tatgagtaaa cttggtctga cagttacctt agaaaaactc atcgagcatc
      421 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
      481 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
      541 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
      601 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
      661 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
      721 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
      781 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
      841 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
      901 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
      961 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     1021 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     1081 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     1141 ccatataaat cagcatccat gttggaattt aatcgcggcc tggagcaaga cgtttcccgt
     1201 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     1261 catgatgata tatttttatc ttgtgcaatg tacatcagag attttgagac acaaccaatt
     1321 attgaaggcc tccctaacgg ggggcctttt tttgtttctg gtctcccgct tggagaacga
     1381 tcgttggctg tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcgctc
     1441 acaatttacg agcaggaatc tgaacgattc gttaccaatt gccatattta aaattcttgt
     1501 ttaaaatgct agcctgagcg ctcaacgggt gtgcttcccg ttctgatgag tccgtgagga
     1561 cgaaagcgcc tctacaaata attttgttta agagtctatg gactatgttt tcacatatga
     1621 gataccagga tggcacgtaa aaccgcagca gaagcagaag aaacccgtca gcgtattatt
     1681 gatgcagcac tggaagtttt tgttgcacag ggtgttagtg atgcaaccct ggatcagatt
     1741 gcacgtaaag ccggtgttac ccgtggtgca gtttattggc attttaatgg taaactggaa
     1801 gttctgcagg cagttctggc aagccgtcag catccgctgg aactggattt tacaccggat
     1861 ctgggtattg aacgtagctg ggaagcagtt gttgttgcaa tgctggatgc agttcatagt
     1921 ccgcagagca aacagtttag cgaaattctg atttatcagg gtctggatga aagcggtctg
     1981 attcataatc gtatggttca ggcaagcgat cgttttctgc agtatattca tcaggttctg
     2041 cgtcatgcag ttacccaggg tgaactgccg attaatctgg atctgcagac cagcattggt
     2101 gtttttaaag gtctgattac cggtctgctg tatgaaggtc tgcgtagcaa agatcagcag
     2161 gcacagatta tcaaagttgc actgggtagc ttttgggcac tgctgcgtga accgcctcgt
     2221 tttctgctgt gtgaagaagc acagattaaa caggtgaaat ccttcgaata attcagccaa
     2281 aaaacttaag accgccggtc ttgtccacta ccttgcagta atgcggtgga caggatcggc
     2341 ggttttcttt tctcttctca aaggtcgctg gagcttgtcc aaccaaatga ttcgttacca
     2401 attgacagtt tctatcgatc tatagataat gctagctacg tactccaccg ttggcttttt
     2461 tccctatcag tgatagagat tgacatccct atcagtgata gagataatga gcacctgagc
     2521 ggtcaacgca tgtgctttgc gttctgatga gacagtgatg tcgaaaccgc ctctacaaat
     2581 aattttgttt aaggagctat ggactatgtt tgaaaggctg aaatactaga tggcacgtac
     2641 cccgagccgt agcagcattg gtagcctgcg tagtccgcat acccataaag caattctgac
     2701 cagcaccatt gaaatcctga aagaatgtgg ttatagcggt ctgagcattg aaagcgttgc
     2761 acgtcgtgcc ggtgcaagca aaccgaccat ttatcgttgg tggaccaata aagcagcact
     2821 gattgccgaa gtgtatgaaa atgaaagcga acaggtgcgt aaatttccgg atctgggtag
     2881 ctttaaagcc gatctggatt ttctgctgcg taatctgtgg aaagtttggc gtgaaaccat
     2941 ttgtggtgaa gcatttcgtt gtgttattgc agaagcacag ctggaccctg caaccctgac
     3001 ccagctgaaa gatcagttta tggaacgtcg tcgtgagatg ccgaaaaaac tggttgaaaa
     3061 tgccattagc aatggtgaac tgccgaaaga taccaatcgt gaactgctgc tggatatgat
     3121 ttttggtttt tgttggtatc gcctgctgac cgaacagctg accgttgaac aggatattga
     3181 agaatttacc ttcctgctga ttaatggtgt ttgtccgggt acacagcgtt aaggaaacac
     3241 agaaaaaagc ccgcacctga cagtgcgggc tttttttttc gaccaaagga ggttactgga
     3301 gcgacgtacg gtggaatctg attcgttacc aattgacatg atacgaaacg taccgtatcg
     3361 ttaaggttac gatagcttct taccggacct gtaggatcgt acaggtttac gcaagaaaat
     3421 ggtttgttac tttcgaataa actgagggtg tctcaaggtg cgtaccttga ctgatgagtc
     3481 cgaaaggacg aaacacccct ctacaaataa ttttgtttaa aatgttccct aataatcagc
     3541 aaagaggtta ctagatggca ggcgcagttg gtcgtccgcg tcgtagtgca ccgcgtcgtg
     3601 caggtaaaaa tccgcgtgaa gaaattctgg atgcaagcgc agaactgttt acccgtcagg
     3661 gttttgcaac caccagtacc catcagattg cagatgcagt tggtattcgt caggcaagcc
     3721 tgtattatca ttttccgagc aaaaccgaaa tctttctgac cctgctgaaa agcaccgttg
     3781 aaccgagcac cgttctggca gaagatctga gcaccctgga tgcaggtccg gaaatgcgtc
     3841 tgtgggcaat tgttgcaagc gaagttcgtc tgctgctgag caccaaatgg aatgttggtc
     3901 gtctgtatca gctgccgatt gttggtagcg aagaatttgc agaatatcat agccagcgtg
     3961 aagcactgac caatgttttt cgtgatctgg caaccgaaat tgttggtgat gatccgcgtg
     4021 cagaactgcc gtttcatatt accatgagcg ttattgaaat gcgtcgcaat gatggtaaaa
     4081 ttccgagtcc gctgagcgca gatagcctgc cggaaaccgc aattatgctg gcagatgcaa
     4141 gcctggcagt tctgggtgca ccgctgcctg cagatcgtgt tgaaaaaacc ctggaactga
     4201 ttaaacaggc agatgcaaaa taactcggta ccaaagacga acaataagac gctgaaaagc
     4261 gtcttttttc gttttggtcc aggttgccgg agtctatgat tggtccagat tcgttaccaa
     4321 ttgacagcta gctcagtcct aggtatatac atacatgctt gtttgtttgt aaactacgat
     4381 agcttcttac cggacctgta ggatcgtaca ggtttacgca agaaaatggt ttgttacttt
     4441 cgaataaact gagtagtcac cggctgtgct tgccggtctg atgagcctgt gaaggcgaaa
     4501 ctacctctac aaataatttt gtttaaaccc ccgagatgaa atacatcctg tttgaggtgt
     4561 gcgaaatggg taaaagccgt gaacagacca tggaaaatat tctgaaagca gccaaaaaga
     4621 aattcggcga acgtggttat gaaggcacca gcattcaaga aattaccaaa gaagccaaag
     4681 ttaacgttgc aatggccagc tattacttta atggcaaaga gaacctgtac tacgaggtgt
     4741 tcaaaaaata cggtctggca aatgaactgc cgaactttct ggaaaaaaac cagtttaatc
     4801 cgattaatgc cctgcgtgaa tatctgaccg tttttaccac ccacattaaa gaaaatccgg
     4861 aaattggcac cctggcctat gaagaaatta tcaaagaaag cgcacgcctg gaaaaaatca
     4921 aaccgtattt tatcggcagc ttcgaacagc tgaaagaaat tctgcaagag ggtgaaaaac
     4981 agggtgtgtt tcactttttt agcatcaacc ataccatcca ttggattacc agcattgttc
     5041 tgtttccgaa attcaaaaaa ttcatcgata gcctgggtcc gaatgaaacc aatgatacca
     5101 atcatgaatg gatgccggaa gatctggtta gccgtattat tagcgcactg accgataaac
     5161 cgaacattta aaacgcatga gaaagccccc ggaagatcac cttccggggg cttttttatt
     5221 gcgcaggtaa tggtcaccat atatcaagtt tacggctagc tcagtcctag gtactatgct
     5281 agctactaga gaaagaggag aaatactaga tggctgaagc gcaaaatgat cccctgctgc
     5341 cgggatactc gtttaatgcc catctggtgg cgggtttaac gccgattgag gccaacggtt
     5401 atctcgattt ttttatcgac cgaccgctgg gaatgaaagg ttatattctc aatctcacca
     5461 ttcgcggtca gggggtggtg aaaaatcagg gacgagaatt tgtttgccga ccgggtgata
     5521 ttttgctgtt cccgccagga gagattcatc actacggtcg tcatccggag gctcgcgaat
     5581 ggtatcacca gtgggtttac tttcgtccgc gcgcctactg gcatgaatgg cttaactggc
     5641 cgtcaatatt tgccaatacg gggttctttc gcccggatga agcgcaccag ccgcatttca
     5701 gcgacctgtt tgggcaaatc attaacgccg ggcaagggga agggcgctat tcggagctgc
     5761 tggcgataaa tctgcttgag caattgttac tgcggcgcat ggaagcgatt aacgagtcgc
     5821 tccatccacc gatggataat cgggtacgcg aggcttgtca gtacatcagc gatcacctgg
     5881 cagacagcaa ttttgatatc gccagcgtcg cacagcatgt ttgcttgtcg ccgtcgcgtc
     5941 tgtcacatct tttccgccag cagttaggga ttagcgtctt aagctggcgc gaggaccaac
     6001 gtatcagcca ggcgaagctg cttttgagca ccacccggat gcctatcgcc accgtcggtc
     6061 gcaatgttgg ttttgacgat caactctatt tctcgcgggt atttaaaaaa tgcaccgggg
     6121 ccagcccgag cgagttccgt gccggttaat aaccaattat tgaaggccgc taacgcagcc
     6181 tttttttgtt tctggtctcc caatggcggc gcgccatcga atggcgcaaa acctttcgcg
     6241 gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatatg aaaccagtaa
     6301 cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga
     6361 accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc
     6421 tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg
     6481 gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat
     6541 ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg
     6601 aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta
     6661 actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg
     6721 cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgagg
     6781 acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt
     6841 tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc
     6901 tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg
     6961 gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg
     7021 ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg
     7081 gtgcggatat ctcggtagtg ggatacgacg ataccgaaga tagctcatgt tatatcccgc
     7141 cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc
     7201 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgccagtc tcactggtga
     7261 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7321 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga caatccagga
     7381 ggaaaaaaat gtccagatta gataaaagta aagtgattaa cagcgcatta gagctgctta
     7441 atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc
     7501 agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg
     7561 agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt
     7621 ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa
     7681 aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag
     7741 cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg
     7801 ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa
     7861 gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc gaattatttg
     7921 atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata tgcggattag
     7981 aaaaacaact taaatgtgaa agtgggtcct aaatcacaca ggaaaggcct cgatgaaaaa
     8041 cataaatgcc gacgacacat acagaataat taataaaatt aaagcttgta gaagcaataa
     8101 tgatattaat caatgcttat ctgatatgac taaaatggta cattgtgaat attatttact
     8161 cgcgatcatt tatcctcatt ctatggttaa atctgatatt tcaatcctag ataattaccc
     8221 taaaaaatgg aggcaatatt atgatgacgc taatttaata aaatatgatc ctatagtaga
     8281 ttattctaac tccaatcatt caccaattaa ttggaatata tttgaaaaca atgctgtaaa
     8341 taaaaaatct ccaaatgtaa ttaaagaagc gaaaacatca ggtcttatca ctgggtttag
     8401 tttccctatt catacggcta acaatggctt cggaatgctt agttttgcac attcagaaaa
     8461 agacaactat atagatagtt tatttttaca tgcgtgtatg aacataccat taattgttcc
     8521 ttctctagtt gataattatc gaaaaataaa tatagcaaat aataaatcaa acaacgattt
     8581 aaccaaaaga gaaaaagaat gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat
     8641 ttcaaaaata ttaggttgca gtgagcgtac tgtcactttc catttaacca atgcgcaaat
     8701 gaaactcaat acaacaaacc gctgccaaag tatttctaaa gcaattttaa caggagcaat
     8761 tgattgccca tactttaaaa attaaccata attggtaacg aatcagacaa ttgacggctc
     8821 gagggagtag catagggttt gcagaatccc tgcttcgtcc atttgacagg cacattatgc
     8881 atcgatgata agctgtcaaa catgagcaga tcctctacgc cggacgcatc gtggccggca
     8941 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     9001 atcgggctcg ccacttcggg ctcatgagca aatattttat ctgaggtgct tcctcgctca
     9061 ctgactcgct gcacgaggca gacctcagcg ctagcggagt gtatactggc ttactatgtt
     9121 ggcactgatg agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg
     9181 tgcgtcagca gaatatgtga tacaggatat attccgcttc ctcgctcact gactcgctac
     9241 gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcccgg
     9301 aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca
     9361 taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa
     9421 cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc
     9481 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc
     9541 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt
//
