LOCUS       sequence-400082-        7305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Number and brightness analysis of EGFR oligomerization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Sankaran J, Balasubramanian H, Tang WH, Ng XW, Rollin A, Wohland T
  TITLE     Simultaneous spatiotemporal super-resolution and multi-parametric 
            fluorescence microscopy.
  JOURNAL   Nat Commun. 2021 Mar 19;12(1):1748. doi: 10.1038/s41467-021-22002-9.
  PUBMED    33741958
REFERENCE   2  (bases 1 to 7305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-19"; volume: "12(1):1748. doi"; pages: " 
            10.1038/s41467-021-22002-9"
COMMENT     Sequence Label: EGFR-mApple
FEATURES             Location/Qualifiers
     source          1..7305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        82..385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..589
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     540..560
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(623..694,695..2557,2558..2626,2627..4252)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     CDS             4271..4978
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     4684..4703
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(5009..5033)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5062..5183
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5073..5092)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5127..5146
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5299..5316)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5470..6058)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5550..5569)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6229..7020,7021..7089))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7090..7194)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7262..7280
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 caactttgta tagaaaagtt gtagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      421 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      481 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      541 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
      601 ccgtcagatc accggtgcca ccatgcgacc ctccgggacg gccggggcag cgctcctggc
      661 gctgctggct gcgctctgcc cggcgagtcg ggctctggag gaaaagaaag tttgccaagg
      721 cacgagtaac aagctcacgc agttgggcac ttttgaagat cattttctca gcctccagag
      781 gatgttcaat aactgtgagg tggtccttgg gaatttggaa attacctatg tgcagaggaa
      841 ttatgatctt tccttcttaa agaccatcca ggaggtggct ggttatgtcc tcattgccct
      901 caacacagtg gagcgaattc ctttggaaaa cctgcagatc atcagaggaa atatgtacta
      961 cgaaaattcc tatgccttag cagtcttatc taactatgat gcaaataaaa ccggactgaa
     1021 ggagctgccc atgagaaatt tacaggaaat cctgcatggc gccgtgcggt tcagcaacaa
     1081 ccctgccctg tgcaacgtgg agagcatcca gtggcgggac atagtcagca gtgactttct
     1141 cagcaacatg tcgatggact tccagaacca cctgggcagc tgccaaaagt gtgatccaag
     1201 ctgtcccaat gggagctgct ggggtgcagg agaggagaac tgccagaaac tgaccaaaat
     1261 catctgtgcc cagcagtgct ccgggcgctg ccgtggcaag tcccccagtg actgctgcca
     1321 caaccagtgt gctgcaggct gcacaggccc ccgggagagc gactgcctgg tctgccgcaa
     1381 attccgagac gaagccacgt gcaaggacac ctgcccccca ctcatgctct acaaccccac
     1441 cacgtaccag atggatgtga accccgaggg caaatacagc tttggtgcca cctgcgtgaa
     1501 gaagtgtccc cgtaattatg tggtgacaga tcacggctcg tgcgtccgag cctgtggggc
     1561 cgacagctat gagatggagg aagacggcgt ccgcaagtgt aagaagtgcg aagggccttg
     1621 ccgcaaagtg tgtaacggaa taggtattgg tgaatttaaa gactcactct ccataaatgc
     1681 tacgaatatt aaacacttca aaaactgcac ctccatcagt ggcgatctcc acatcctgcc
     1741 ggtggcattt aggggtgact ccttcacaca tactcctcct ctggatccac aggaactgga
     1801 tattctgaaa accgtaaagg aaatcacagg gtttttgctg attcaggctt ggcctgaaaa
     1861 caggacggac ctccatgcct ttgagaacct agaaatcata cgcggcagga ccaagcaaca
     1921 tggtcagttt tctcttgcag tcgtcagcct gaacataaca tccttgggat tacgctccct
     1981 caaggagata agtgatggag atgtgataat ttcaggaaac aaaaatttgt gctatgcaaa
     2041 tacaataaac tggaaaaaac tgtttgggac ctccggtcag aaaaccaaaa ttataagcaa
     2101 cagaggtgaa aacagctgca aggccacagg ccaggtctgc catgccttgt gctcccccga
     2161 gggctgctgg ggcccggagc ccagggactg cgtctcttgc cggaatgtca gccgaggcag
     2221 ggaatgcgtg gacaagtgca accttctgga gggtgagcca agggagtttg tggagaactc
     2281 tgagtgcata cagtgccacc cagagtgcct gcctcaggcc atgaacatca cctgcacagg
     2341 acggggacca gacaactgta tccagtgtgc ccactacatt gacggccccc actgcgtcaa
     2401 gacctgcccg gcaggagtca tgggagaaaa caacaccctg gtctggaagt acgcagacgc
     2461 cggccatgtg tgccacctgt gccatccaaa ctgcacctac ggatgcactg ggccaggtct
     2521 tgaaggctgt ccaacgaatg ggcctaagat cccgtccatc gccactggga tggtgggggc
     2581 cctcctcttg ctgctggtgg tggccctggg gatcggcctc ttcatgcgaa ggcgccacat
     2641 cgttcggaag cgcacgctgc ggaggctgct gcaggagagg gagcttgtgg agcctcttac
     2701 acccagtgga gaagctccca accaagctct cttgaggatc ttgaaggaaa ctgaattcaa
     2761 aaagatcaaa gtgctgggct ccggtgcgtt cggcacggtg tataagggac tctggatccc
     2821 agaaggtgag aaagttaaaa ttcccgtcgc tatcaaggaa ttaagagaag caacatctcc
     2881 gaaagccaac aaggaaatcc tcgatgaagc ctacgtgatg gccagcgtgg acaaccccca
     2941 cgtgtgccgc ctgctgggca tctgcctcac ctccaccgtg caactcatca cgcagctcat
     3001 gcccttcggc tgcctcctgg actatgtccg ggaacacaaa gacaatattg gctcccagta
     3061 cctgctcaac tggtgtgtgc agatcgcaaa gggcatgaac tacttggagg accgtcgctt
     3121 ggtgcaccgc gacctggcag ccaggaacgt actggtgaaa acaccgcagc atgtcaagat
     3181 cacagatttt gggctggcca aactgctggg tgcggaagag aaagaatacc atgcagaagg
     3241 aggcaaagtg cctatcaagt ggatggcatt ggaatcaatt ttacacagaa tctataccca
     3301 ccagagtgat gtctggagct acggggtgac cgtttgggag ttgatgacct ttggatccaa
     3361 gccatatgac ggaatccctg ccagcgagat ctcctccatc ctggagaaag gagaacgcct
     3421 ccctcagcca cccatatgta ccatcgatgt ctacatgatc atggtcaagt gctggatgat
     3481 agacgcagat agtcgcccaa agttccgtga gttgatcatc gaattctcca aaatggcccg
     3541 agacccccag cgctaccttg tcattcaggg ggatgaaaga atgcatttgc caagtcctac
     3601 agactccaac ttctaccgtg ccctgatgga tgaagaagac atggacgacg tggtggatgc
     3661 cgacgagtac ctcatcccac agcagggctt cttcagcagc ccctccacgt cacggactcc
     3721 cctcctgagc tctctgagtg caaccagcaa caattccacc gtggcttgca ttgatagaaa
     3781 tgggctgcaa agctgtccca tcaaggaaga cagcttcttg cagcgataca gctcagaccc
     3841 cacaggcgcc ttgactgagg acagcataga cgacaccttc ctcccagtgc ctgaatacat
     3901 aaaccagtcc gttcccaaaa ggcccgctgg ctctgtgcag aatcctgtct atcacaatca
     3961 gcctctgaac cccgcgccca gcagagaccc acactaccag gacccccaca gcactgcagt
     4021 gggcaacccc gagtatctca acactgtcca gcccacctgt gtcaacagca cattcgacag
     4081 ccctgcccac tgggcccaga aaggcagcca ccaaattagc ctggacaacc ctgactacca
     4141 gcaggacttc tttcccaagg aagccaagcc aaatggcatc tttaagggct ccacagctga
     4201 aaatgcagaa tacctaaggg tcgcgccaca aagcagtgaa tttattggag caggaggtgg
     4261 cgggactagt atggtgagca agggcgagga gaataacatg gccatcatca aggagttcat
     4321 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     4381 gggcgagggc cgcccctacg aggcctttca gaccgctaag ctgaaggtga ccaagggtgg
     4441 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggtcta
     4501 cattaagcac ccagccgaca tccccgacta cttcaagctg tccttccccg agggcttcag
     4561 gtgggagcgc gtgatgaact tcgaggacgg cggcattatt cacgttaacc aggactcctc
     4621 cctgcaggac ggcgtgttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     4681 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccgaggagc ggatgtaccc
     4741 cgaggacggc gccctgaaga gcgagatcaa gaagaggctg aagctgaagg acggcggcca
     4801 ctacgccgcc gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     4861 ctacatcgtc gacatcaagt tggacatcgt gtcccacaac gaggactaca ccatcgtgga
     4921 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggg
     4981 tggcggaggg tccgctagct aaaagcttac ccagctttct tgtacaaagt ggtgatggcc
     5041 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     5101 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     5161 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     5221 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtagcggcc
     5281 gcggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5341 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5401 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5461 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5521 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5581 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctctc
     5641 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     5701 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5761 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5821 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5881 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     5941 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6001 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6061 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6121 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6181 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6241 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6301 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6361 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6421 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6481 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6541 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6601 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6661 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6721 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6781 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6841 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6901 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6961 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7021 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7081 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7141 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7201 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     7261 aaataggcgt atcacgaggc cctttcgtcg gcgcgccgcg gccgc
//
