LOCUS       sequence-400097-        2945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  the promoter can be recognized by PadR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Jiang T, Li C, Yan Y
  TITLE     Optimization of a p-Coumaric Acid Biosensor System for Versatile 
            Dynamic Performance.
  JOURNAL   ACS Synth Biol. 2021 Jan 15;10(1):132-144. doi: 
            10.1021/acssynbio.0c00500. Epub 2020 Dec 30.
  PUBMED    33378169
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00500"; journalName: "ACS Synth Biol"; date: 
            "2021-01-15- 15"; volume: "10"; issue: "1"; pages: "132-144"
COMMENT     Sequence Label: pZE-P9-egfp
FEATURES             Location/Qualifiers
     source          1..2945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             83..94
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             101..817
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(131..159)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     754..774
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      829..915
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1080..1668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1160..1179)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1756..1850)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1880..2671,2672..2740))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2503..2522
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2741..2845)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2913..2931
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagtaaa ctctggcggt gttgacataa ataccactgg cggtgatact gaacatgtaa
       61 atagttacat gatgaattca ttaaagagga gaaaggtatc atgagtaaag gagaagaact
      121 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
      181 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
      241 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcgcgtatgg
      301 tcttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc
      361 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
      421 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
      481 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
      541 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat
      601 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
      661 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
      721 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc
      781 tgggattaca catggcatgg atgaactata caaatagtct agaggcatca aataaaacga
      841 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      901 ctgagtagga caaatccgcc gccctagacc tagggcgttc ggctgcggcg agcggtatca
      961 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1021 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1081 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1141 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1201 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1261 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1321 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1381 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1441 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1501 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1561 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1621 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1681 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1741 atgactagtg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     1801 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     1861 cgtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1921 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1981 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     2041 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2101 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2161 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     2221 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     2281 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     2341 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     2401 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2461 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     2521 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     2581 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     2641 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     2701 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     2761 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     2821 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     2881 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     2941 ttcac
//
