LOCUS       sequence-400288-       12142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2067
FEATURES             Location/Qualifiers
     source          1..12142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7999..8587
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8052..8072)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8470..8481)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8659..8892
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8970..9091
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9007..9026)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9061..9080
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9131..9266
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9286..9305)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9287..9305)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9315..9332)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9315..9331)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9324..9346)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9473..9928
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9560..9579)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9770..9791
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9954..10058
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10059..10127,10128..10919)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10277..10296)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11090..11678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11579..11598
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11832..11849
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11966..11987
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12002..12007,12008..12025,12026..12032)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12040..12056
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12045..12067
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12064..12080
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12064..12080
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12099..12119
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12101..12119
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggac ctccgaggcc tccgcccagt
     4141 gcctggcggc tacttccctg agaatgtcaa ggccatgacc agcctgcggt ggctgaagct
     4201 gaaccgcact ggcctctgct acctgcccga ggagctggcc gccctgcaga agctggaaca
     4261 cttgtctgtg agccacaaca acctgaccac gcttcatggg gagctgtcca gcctgccatc
     4321 gctgcgcgcc atcgtggccc gagccaacag tctgaagaat tccggagtcc ccgatgacat
     4381 cttcaagcta gatgatctct cagtcctgga cttgagccac aaccagctga cagagtgccc
     4441 gcgggagctg gagaacgcca agaacatgct ggtgctgaac ctcagccaca acagcatcga
     4501 caccatcccc aaccagctct tcatcaacct cactgaccta ctatacctgg acctcagcga
     4561 gaaccgcctg gagagcctgc ccccgcagat gcgccgcctg gtgcacctgc agacgctcgt
     4621 gctcaatgga aaccccctgc tgcatgcaca gctccggcag ctcccagcga tgacggccct
     4681 gcagaccctg cacctgcgga gcacccagcg cacccagagc aacctgccca ccagcctgga
     4741 gggtctgagc aacctcgcag acgtggatct gtcctgcaat gacctgacac gggtgcccga
     4801 gtgtctgtac accctcccca gcctgcgccg cctcaacctc agcagcaacc agatcacgga
     4861 gctgtccctg tgcatagacc agtgggtgca cgtggaaact ctgaacctgt cccgaaatca
     4921 gctcacctca ctgccctcag ccatttgcaa gctgagcaag ctgaagaagc tgtacctgaa
     4981 ttccaacaag ctggactttg acgggctgcc ctcaggcatt ggcaagctca ccaacctgga
     5041 agagttcatg gctgccaaca acaacctgga gctggtccct gaaagtctct gcaggtgccc
     5101 aaagctgagg aaacttgtcc tgaacaagaa ccacctggtg accctcccag aagccatcca
     5161 tttcctgacg gagatcgagg tcctggatgt gcgggagaac cccaacctgg tcatgccgcc
     5221 caagcccgca gaccgtgccg ctgagtggta caacatcgac ttctcgctgc agaaccagct
     5281 gcggctagcg ggtgcctctc ctgctaccgt ggctgcagct gcagctgcag ggagtgggcc
     5341 caaggaccct atggctcgca agatgcgact gcggaggcgc aaggattcag cccaggatga
     5401 ccaggccaag caggtgctga agggcatgtc agatgttgcc caggagaaga acaaaaagca
     5461 ggaggagagc gcagatgccc gggcccccag cgggaaggtg cggcgttggg accagggcct
     5521 ggagaagccc cgccttgact actccgagtt cttcacggag gacgtgggcc agctgcccgg
     5581 actgaccatc tggcagatag agaacttcgt gcctgtgctg gtggaggaag ccttccacgg
     5641 caagttctac gaggctgact gctacattgt gctcaagacc tttctggatg acagcggctc
     5701 cctcaactgg gagatctact actggattgg cggggaggcc acactcgaca agaaagcttg
     5761 ctctgccatc cacgctgtca acttgcgcaa ctacctgggt gctgagtgcc gcactgtccg
     5821 ggaggagatg ggcgatgaga gcgaggagtt cctgcaggtg tttgacaacg acatctccta
     5881 cattgagggt ggaacagcca gtggcttcta cactgtggaa gacacacact atgtcaccag
     5941 gatgtatcgt gtgtatggga aaaagaacat caagttggag cctgtgcccc tcaaggggac
     6001 ctctctggac ccaaggtttg ttttcctgct ggaccgaggg ctagacatct acgtatggcg
     6061 gggggcccag gccacactga gcagcaccac caaggccagg ctctttgcag agaaaattaa
     6121 caagaatgag cggaaaggga aggctgagat cacactgctg gtgcagggcc aggagctccc
     6181 agagttctgg gaggcactgg gtggggagcc ctctgagatc aagaagcacg tgcctgaaga
     6241 cttctggccg ccgcagccca agctgtacaa ggtgggcctg ggcttgggct acctggagct
     6301 gccacagatc aactacaagc tctccgtgga acataagcag cgtcccaagg tggagctgat
     6361 gccaagaatg cggctgctgc agagtctgct ggacacgcgc tgcgtgtaca ttctggactg
     6421 ttggtccgac gtgttcatct ggctcggccg caagtccccg cgcctggtgc gcgctgccgc
     6481 cctcaagctg ggtcaggagc tgtgcgggat gctgcaccgg ccacgccatg ccacggtcag
     6541 ccgcagcctc gagggcaccg aggcgcaggt gttcaaggcc aagttcaaga attgggacga
     6601 tgtgttgacg gtggactaca cacgcaatgc ggaggccgtg ctgcagagcc cgggtctctc
     6661 cgggaaggtg aaacgcgacg ccgagaagaa agaccagatg aaggctgacc tcactgcgct
     6721 tttcctgccg cggcagccgc ccatgtcgct ggccgaggcg gagcagctga tggaggagtg
     6781 gaacgaagac ctagacggca tggagggttt cgtgctggag ggcaagaagt ttgcgcggct
     6841 gccggaagag gagtttggcc acttctacac gcaggactgc tacgtcttcc tctgcaggta
     6901 ctgggtgcct gtggagtacg aggaggagga aaagaaggaa gacaaggagg agaaggccga
     6961 gggcaaagaa ggcgaggaag caaccgctga ggcagaggag aagcagccag aggaggactt
     7021 ccagtgcatc gtgtacttct ggcagggccg tgaagcctcc aatatgggct ggctcacctt
     7081 caccttcagc ctgcaaaaga agttcgagag cctcttccct gggaagctgg aggtggtacg
     7141 catgacgcag cagcaggaga accccaagtt cctgtcccat ttcaagagga agttcatcat
     7201 ccaccggggc aagaggaagg cggtccaggg cgcccaacag cccagcctct accagatccg
     7261 caccaacggc agcgccctct gcacccggtg catccagatc aacaccgact ccagcctcct
     7321 caactccgag ttctgcttca tcctcaaggt tccctttgag agtgaggaca accagggcat
     7381 cgtgtatgcc tgggtgggcc gggcatcaga ccctgacgaa gccaagttgg cagaagacat
     7441 cctgaacacc atgtttgaca cctcctacag caagcaggtt atcaacgaag gtgaggagcc
     7501 tgagaacttc ttctgggtgg gcattggggc acagaagccc tatgatgacg atgccgagta
     7561 catgaaacac acacgtctct tccggtgctc caacgagaag ggctactttg cagtgactga
     7621 gaaatgctcc gacttttgcc aagatgacct ggcagatgat gacatcatgt tgctagacaa
     7681 tggccaagag gtctacatgt gggtggggac ccagactagc caggtggaga tcaagctgag
     7741 cctgaaggcc tgccaggtat atatccagca catgcggtcc aaggaacatg agcggccgcg
     7801 ccggctgcgc ctggtccgca agggcaatga gcagcacgcc tttacccgct gcttccacgc
     7861 ctggagcgcc ttctgcaagg ccctggccta gtaatgaact agtccgtaac ttgaaagtat
     7921 ttcgatttct tggctttata tatcttgtgg aaaggacgaa aggtctgtga tgcgagtcct
     7981 aatacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     8041 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     8101 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     8161 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     8221 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     8281 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     8341 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     8401 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8461 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8521 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     8581 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     8641 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     8701 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8761 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     8821 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8881 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     8941 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     9001 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     9061 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     9121 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     9181 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     9241 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     9301 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     9361 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     9421 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     9481 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     9541 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     9601 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     9661 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     9721 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     9781 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     9841 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     9901 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     9961 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    10021 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
    10081 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
    10141 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
    10201 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
    10261 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
    10321 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
    10381 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
    10441 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
    10501 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
    10561 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
    10621 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
    10681 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
    10741 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
    10801 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
    10861 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
    10921 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    10981 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    11041 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    11101 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    11161 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    11221 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    11281 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    11341 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    11401 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    11461 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    11521 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    11581 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    11641 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    11701 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    11761 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    11821 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    11881 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    11941 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    12001 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    12061 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
    12121 aacaaaagct ggagctgcaa gc
//
