LOCUS       sequence-400303-       12010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12010)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2066
FEATURES             Location/Qualifiers
     source          1..12010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7867..8455
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7920..7940)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8338..8349)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8527..8760
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8838..8959
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8875..8894)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8929..8948
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8999..9134
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9154..9173)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9155..9173)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9183..9200)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9183..9199)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9192..9214)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9341..9796
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9428..9447)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9638..9659
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9822..9926
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9927..9995,9996..10787)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10145..10164)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10958..11546
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11447..11466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11700..11717
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11834..11855
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11870..11875,11876..11893,11894..11900)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11908..11924
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11913..11935
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11932..11948
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11932..11948
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11967..11987
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11969..11987
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag gccaccgggg tgctgccgtt
     4141 cgtgcgtggc gtggacctca gcggcaacga cttcaagggc ggctacttcc ctgagaatgt
     4201 caaggccatg accagcctgc ggtggctgaa gctgaaccgc actggcctct gctacctgcc
     4261 cgaggagctg gccgccctgc agaagctgga acacttgtct gtgagccaca acaacctgac
     4321 cacgcttcat ggggagctgt ccagcctgcc atcgctgcgc gccatcgtgg cccgagccaa
     4381 cagtctgaag aattccggag tccccgatga catcttcaag ctagatgatc tctcagtcct
     4441 ggacttgagc cacaaccagc tgacagagtg cccgcgggag ctggagaacg ccaagaacat
     4501 gctggtgctg aacctcagcc acaacaggca gctcccagcg atgacggccc tgcagaccct
     4561 gcacctgcgg agcacccagc gcacccagag caacctgccc accagcctgg agggtctgag
     4621 caacctcgca gacgtggatc tgtcctgcaa tgacctgaca cgggtgcccg agtgtctgta
     4681 caccctcccc agcctgcgcc gcctcaacct cagcagcaac cagatcacgg agctgtccct
     4741 gtgcatagac cagtgggtgc acgtggaaac tctgaacctg tcccgaaatc agctcacctc
     4801 actgccctca gccatttgca agctgagcaa gctgaagaag ctgtacctga attccaacaa
     4861 gctggacttt gacgggctgc cctcaggcat tggcaagctc accaacctgg aagagttcat
     4921 ggctgccaac aacaacctgg agctggtccc tgaaagtctc tgcaggtgcc caaagctgag
     4981 gaaacttgtc ctgaacaaga accacctggt gaccctccca gaagccatcc atttcctgac
     5041 ggagatcgag gtcctggatg tgcgggagaa ccccaacctg gtcatgccgc ccaagcccgc
     5101 agaccgtgcc gctgagtggt acaacatcga cttctcgctg cagaaccagc tgcggctagc
     5161 gggtgcctct cctgctaccg tggctgcagc tgcagctggg agtgggccca aggaccctat
     5221 ggctcgcaag atgcgactgc ggaggcgcaa ggattcagcc caggatgacc aggccaagca
     5281 ggtgctgaag ggcatgtcag atgttgccca ggagaagaac aaaaagcagg aggagagcgc
     5341 agatgcccgg gcccccagcg ggaaggtgcg gcgttgggac cagggcctgg agaagccccg
     5401 ccttgactac tccgagttct tcacggagga cgtgggccag ctgcccggac tgaccatctg
     5461 gcagatagag aacttcgtgc ctgtgctggt ggaggaagcc ttccacggca agttctacga
     5521 ggctgactgc tacattgtgc tcaagacctt tctggatgac agcggctccc tcaactggga
     5581 gatctactac tggattggcg gggaggccac actcgacaag aaagcttgct ctgccatcca
     5641 cgctgtcaac ttgcgcaact acctgggtgc tgagtgccgc actgtccggg aggagatggg
     5701 cgatgagagc gaggagttcc tgcaggtgtt tgacaacgac atctcctaca ttgagggtgg
     5761 aacagccagt ggcttctaca ctgtggaaga cacacactat gtcaccagga tgtatcgtgt
     5821 gtatgggaaa aagaacatca agttggagcc tgtgcccctc aaggggacct ctctggaccc
     5881 aaggtttgtt ttcctgctgg accgagggct agacatctac gtatggcggg gggcccaggc
     5941 cacactgagc agcaccacca aggccaggct ctttgcagag aaaattaaca agaatgagcg
     6001 gaaagggaag gctgagatca cactgctggt gcagggccag gagctcccag agttctggga
     6061 ggcactgggt ggggagccct ctgagatcaa gaagcacgtg cctgaagact tctggccgcc
     6121 gcagcccaag ctgtacaagg tgggcctggg cttgggctac ctggagctgc cacagatcaa
     6181 ctacaagctc tccgtggaac ataagcagcg tcccaaggtg gagctgatgc caagaatgcg
     6241 gctgctgcag agtctgctgg acacgcgctg cgtgtacatt ctggactgtt ggtccgacgt
     6301 gttcatctgg ctcggccgca agtccccgcg cctggtgcgc gctgccgccc tcaagctggg
     6361 tcaggagctg tgcgggatgc tgcaccggcc acgccatgcc acggtcagcc gcagcctcga
     6421 gggcaccgag gcgcaggtgt tcaaggccaa gttcaagaat tgggacgatg tgttgacggt
     6481 ggactacaca cgcaatgcgg aggccgtgct gcagagcccg ggtctctccg ggaaggtgaa
     6541 acgcgacgcc gagaagaaag accagatgaa ggctgacctc actgcgcttt tcctgccgcg
     6601 gcagccgccc atgtcgctgg ccgaggcgga gcagctgatg gaggagtgga acgaagacct
     6661 agacggcatg gagggtttcg tgctggaggg caagaagttt gcgcggctgc cggaagagga
     6721 gtttggccac ttctacacgc aggactgcta cgtcttcctc tgcaggtact gggtgcctgt
     6781 ggagtacgag gaggaggaaa agaaggaaga caaggaggag aaggccgagg gcaaagaagg
     6841 cgaggaagca accgctgagg cagaggagaa gcagccagag gaggacttcc agtgcatcgt
     6901 gtacttctgg cagggccgtg aagcctccaa tatgggctgg ctcaccttca ccttcagcct
     6961 gcaaaagaag ttcgagagcc tcttccctgg gaagctggag gtggtacgca tgacgcagca
     7021 gcaggagaac cccaagttcc tgtcccattt caagaggaag ttcatcatcc accggggcaa
     7081 gaggaaggcg gtccagggcg cccaacagcc cagcctctac cagatccgca ccaacggcag
     7141 cgccctctgc acccggtgca tccagatcaa caccgactcc agcctcctca actccgagtt
     7201 ctgcttcatc ctcaaggttc cctttgagag tgaggacaac cagggcatcg tgtatgcctg
     7261 ggtgggccgg gcatcagacc ctgacgaagc caagttggca gaagacatcc tgaacaccat
     7321 gtttgacacc tcctacagca agcaggttat caacgaaggt gaggagcctg agaacttctt
     7381 ctgggtgggc attggggcac agaagcccta tgatgacgat gccgagtaca tgaaacacac
     7441 acgtctcttc cggtgctcca acgagaaggg ctactttgca gtgactgaga aatgctccga
     7501 cttttgccaa gatgacctgg cagatgatga catcatgttg ctagacaatg gccaagaggt
     7561 ctacatgtgg gtggggaccc agactagcca ggtggagatc aagctgagcc tgaaggcctg
     7621 ccaggtatat atccagcaca tgcggtccaa ggaacatgag cggccgcgcc ggctgcgcct
     7681 ggtccgcaag ggcaatgagc agcacgcctt tacccgctgc ttccacgcct ggagcgcctt
     7741 ctgcaaggcc ctggcctagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     7801 gctttatata tcttgtggaa aggacgaggt aacggatctt acagtcaaga tacgcgttaa
     7861 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     7921 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     7981 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     8041 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     8101 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     8161 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     8221 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     8281 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     8341 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     8401 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     8461 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     8521 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     8581 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8641 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     8701 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     8761 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     8821 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     8881 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8941 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     9001 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     9061 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     9121 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     9181 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     9241 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     9301 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     9361 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     9421 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     9481 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     9541 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     9601 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     9661 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     9721 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     9781 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     9841 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     9901 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     9961 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    10021 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    10081 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    10141 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    10201 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    10261 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    10321 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    10381 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    10441 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    10501 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    10561 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    10621 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    10681 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    10741 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    10801 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    10861 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    10921 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    10981 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    11041 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    11101 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    11161 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    11221 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    11281 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    11341 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    11401 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    11461 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    11521 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    11581 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    11641 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    11701 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    11761 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    11821 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    11881 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    11941 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    12001 agctgcaagc
//
