LOCUS       sequence-400355-       13594 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13594)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1367
FEATURES             Location/Qualifiers
     source          1..13594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    9451..10039
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9504..9524)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9922..9933)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10111..10344
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10422..10543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10459..10478)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10513..10532
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10583..10718
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10738..10757)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10739..10757)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10767..10784)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10767..10783)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10776..10798)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10925..11380
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11012..11031)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11222..11243
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11406..11510
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11511..11579,11580..12371)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11729..11748)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12542..13130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13031..13050
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13284..13301
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13418..13439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13454..13459,13460..13477,13478..13484)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13492..13508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13497..13519
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13516..13532
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13516..13532
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13551..13571
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13553..13571
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgacg tcaagttcgc ccgctggctt
     4141 ggaaggttca gacctatctt ccatcaacac catgatgtcg gcggtcatga gtgtagggaa
     4201 ggtcacagag aatggcggga gcccccaggg gatcaagtcc ccctcgaagc ctccaggacc
     4261 aaatcggatt ggcagaagga accaggaaac gaaagaggag aagtcttcct ataactgccc
     4321 cctgtgtgag aagatttgca ctacccagca ccagctgacc atgcacattc gccagcacaa
     4381 cacagacact ggaggagccg accactcatg cagcatctgc ggaaagtcac tgagctcggc
     4441 cagctccctc gatcgccaca tgctggtgca ctctggcgag aggccttaca agtgcactgt
     4501 gtgtggccag tcatttacca ccaatgggaa catgcacaga catatgaaga tccatgagaa
     4561 ggaccctaac agtgccacag ccacagcccc tccatctcct ctgaaacgta ggcgattgtc
     4621 ctccaagagg aaactgagtc acgatgccga gtcagagaga gaagacccag caccagctaa
     4681 aaagatggta gaagacgggc agtcaggtga cttggagaag aaagctgatg aagtctttca
     4741 ctgcccagta tgtttcaagg agtttgtttg caagtatgga ctggagaccc acatggagac
     4801 ccattcagat aacccactaa gatgtgacat ttgttgtgtc acctttcgaa cacatcgagg
     4861 actgctgcgt cacaacgcgc ttgtccacaa acaacttccc agggatgcaa tgggcagacc
     4921 tttcatacag aacaaccctt caattcctgc tggcttccac gacttaggat tcacggactt
     4981 ctcctgtagg aagtttcctc gcatttctca ggcctggtgc gaaacaaacc tgcggaggtg
     5041 catcagcgag caacaccgtt ttgtctgcga cacctgtgac aaggcgttcc ccatgctctg
     5101 ctcactggct ctgcacaagc agacccatgt ggcggcagac cagggtcaag aaaagccgca
     5161 ggccacgccc ctgcctggtg acgccctgga ccagaagggc ttcctggcct tgcttggcct
     5221 gcagcacacc aaagacgtca ggcctgcccc cgccgaggag cccctgccgg atgacaacca
     5281 ggcaattcag ctccagacac tcaagtgtca gctacctcag gaccccggct gcaccaacct
     5341 gctgagcctg tcacctttcg aagctgcttc cctaggcggt tctctcacag ttctccccgc
     5401 gaccaaggac agcataaagc acctgtccct gcagcccttc cagaagggct tcatcatcca
     5461 gcctgacagc agcattgtgg tcaagcccat ctctggcgag tcggccatcg agctggcaga
     5521 catccagcaa attctgaaga tggcagcctc ggctccccct cagatcagtc ttccgccctt
     5581 ctccaaggcc cctgccgccc cactgcaggc gatcttcaag cacatgcccc ctctgaagcc
     5641 aaagcccctg gtcacaccac ggacggtggt ggccacctcc acgcccccgc ctctcatcaa
     5701 cgcccagcag gcttccccgg gctgtatcag ccccagcctg ccgccaccgc ccctgaagct
     5761 cctcaaaggc tcagtggagg cggcctccaa cgcccacctg ctgcagtcca agtccgggac
     5821 ccagccccac gcggccacgc ggctctccct gcagcagccg cgggcggagc tgccgggcca
     5881 gcctgagatg aagacgcagc tggagcagga cagcatcatc gaggccctgc tgccgctgag
     5941 catggaggcc aagatcaagc aggagatcac agagggggaa ctcaaggcct tcatgacagc
     6001 gcccggcggc aagaagacgc ccgccatgcg caaggtgctc tacccctgcc gcttctgcaa
     6061 ccaggtgttt gccttctcgg gggtcttgcg tgcccacgtg cgctcccacc tgggcatctc
     6121 gccataccag tgcaacatct gcgactacat cgccgccgac aaggccgcgc tcatccgcca
     6181 cctgcgcacg cacagtgggg agcggcccta catttgcaag atctgccact accccttcac
     6241 tgtcaaagcc aactgcgagc ggcacctgcg caagaagcac ctcaaggcca cccgcaagga
     6301 tatcgagaag aacatcgagt atgtgagtag cagcgcggcc gagctggtgg acgccttctg
     6361 cgccccggac accgtgtgcc ggctgtgcgg cgaggacctc aagcactatc gtgccctgcg
     6421 catccacatg cgcacgcact gcggccgcgg cctgggcggg ggccacaagg gccgcaagcc
     6481 cttcgagtgc aaggagtgca gcgccgcgtt cgcggccaag cgcaactgca tccaccacat
     6541 cctcaagcag cacctgcacg tgcccgagca ggacatcgag agctacgtgc tggccgccga
     6601 cggcctgggc cccgcagagg cgccggccgc tgaggcgtcg gggcgcgggg aggacagtgg
     6661 ctgcgctgcc cttggtgact gcaagcccct cactgccttc ctggaacccc agaacggctt
     6721 tcttcacagg ggccccaccc agcctccacc tccccatgtc tcgatcaagt tggagcccgc
     6781 cagtagcttt gcggtggact tcaatgagcc cctggacttc tcgcagaagg gcctggccct
     6841 ggtccaagtg aagcaggaaa acatctcctt tctgagccct tcttccctgg tcccctatga
     6901 ctgctccatg gagcccatcg acctgtccat ccccaagaac ttcaggaaag gggacaagga
     6961 tttggccact cccagcgaag ccaagaagcc tgaggaggag gcggggagca gcgagcagcc
     7021 ctctccctgc ccagcacccg gcccttctct tcctgtaact ttggggccca gcggaatcct
     7081 ggaaagcccc atggcccctg ctccggcggc caccccggaa cccccagcac agcccctgca
     7141 gggccctgtt cagctggcgg tcccaatcta ctcctcagcc ctggtcagca gccctccact
     7201 cgtgggcagc tcagccctcc tgagtggcac agccttgctg cgtccactgc ggcccaagcc
     7261 cccgctgctt ttgccaaagc cccccgtgac agaagagctg cccccgctgg cctccattgc
     7321 ccagatcatc tcatctgtat cctcggcccc caccctgctg aaaaccaagg tggcggaccc
     7381 agggcccgca agcactggca gtaacaccac ggcttcagac agcttaggag gttctgtccc
     7441 caaagccgcc accaccgcca cccccgctgc caccaccagc ccaaaagagt ctagtgagcc
     7501 tcccgctcca gccagcagcc cagaggctgc ctctcccacc gagcagggcc cagcgggcac
     7561 gtcgaagaag aggggccgga aaagggggat gaggagccga ccccgcgcca acagcggcgg
     7621 ggtggacctg gactccagcg gggagtttgc cagcatcgag aagatgctgg ccaccacaga
     7681 caccaacaag ttcagtccgt ttctgcagac agcggaggac aacactcagg atgaggtggc
     7741 cggagcccct gccgaccacc atgggcccag tgatgaagag cagggcagtc ccccagaaga
     7801 caagctgctg agggccaagc ggaactcgta caccaactgc ctgcagaaga tcacctgtcc
     7861 ccactgtccc cgggttttcc cttgggccag ctccctacag aggcacatgc tcacacacac
     7921 tggtcagaaa cccttccctt gtcaaaaatg cgatgccttc ttttctacca aatctaactg
     7981 tgaacgccac cagttgcgca aacacggagt taccacctgt tccctgagaa gaaacgggct
     8041 tatcccccag tcaaaagaga gtgatgttgg atcccatgat agcacagaca gtcagtcgga
     8101 tgcggagact gcagccgccg cgggcgaagt gctagacctc acctcacggg acagagagca
     8161 gccgtcggag ggcgccactg agctccgcca ggtcgcaggg gatgcgcctg tggagcaggc
     8221 cacggcggaa acggcctcgc cggtgcaccg ggaagagcac gggcgtgggg agagccatga
     8281 gccggaggag gagcatggca ctgaggagag cactggggac gccgacggcg cggaagagga
     8341 cgcgtcgagc aaccagagcc tggacctgga cttcgccacc aagctcatgg acttcaagct
     8401 ggcggagggc gacggcgagg caggcgccgg gggcgcggcc tcgcaggagc agaagctcgc
     8461 ctgcgacacc tgtgggaaga gcttcaagtt cctgggcacc ctgagccgcc accggaaggc
     8521 gcacggccgc caggagccca aggacgagaa gggagatggc gccagcactg cagaggaggg
     8581 gccccagccc gcccctgaac aggaggagaa gccccccgag accccggcag aggtggtgga
     8641 gtcggccccg ggtgccgggg aggccccggc ggaaaagctc gcggaggaga cggagggccc
     8701 ctccgacggg gagagcgcgg ccgagaaaag gtcctcagag aagagcgacg atgacaagaa
     8761 accaaagaca gactccccca aaagcgtggc cagcaaggca gacaagagga agaaggtctg
     8821 cagcgtgtgc aacaagcggt tctggtcgct gcaggacctg acccggcaca tgcgctccca
     8881 cacaggggaa aggccataca aatgtcagac ctgcgagcga accttcacct tgaagcacag
     8941 cctggttcgc caccagcgga tccaccagaa agccaggcat gccaaacacc acgggaagga
     9001 cagcgacaag gaagagcggg gtgaggagga cagcgagaat gagtccaccc acagcggcaa
     9061 caacgccgtc tcagagaacg aggctgagct ggctcccaat gccagcaacc acatggctgt
     9121 cacccggagc cggaaggagg gcttggccag tgccaccaag gactgcagcc acagggagga
     9181 gaaggtcacg gcagggtggc cgtctgagcc tggccagggt gaccttaacc cagagagccc
     9241 ggcggccctg gggcaggacc tgctggagcc gcgcagcaag aggcctgccc acccaatcct
     9301 ggccacagct gatggcgcct cccagctcgt ggggatggag tagtaatgaa ctagtccgta
     9361 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aacgttgaga
     9421 acttacagac acaacacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     9481 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     9541 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     9601 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     9661 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     9721 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     9781 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     9841 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     9901 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     9961 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
    10021 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
    10081 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
    10141 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
    10201 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    10261 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    10321 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
    10381 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
    10441 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    10501 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
    10561 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
    10621 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
    10681 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
    10741 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
    10801 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    10861 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
    10921 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
    10981 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
    11041 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
    11101 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
    11161 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
    11221 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
    11281 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
    11341 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
    11401 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    11461 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
    11521 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    11581 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    11641 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    11701 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
    11761 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    11821 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    11881 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    11941 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    12001 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    12061 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    12121 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    12181 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    12241 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    12301 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    12361 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    12421 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    12481 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    12541 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    12601 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    12661 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    12721 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    12781 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    12841 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    12901 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    12961 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    13021 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    13081 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    13141 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    13201 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    13261 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    13321 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    13381 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    13441 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    13501 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
    13561 ctcactaaag ggaacaaaag ctggagctgc aagc
//
