LOCUS       sequence-400357-       12058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the MECOM transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12058)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0341
FEATURES             Location/Qualifiers
     source          1..12058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7915..8503
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7968..7988)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8386..8397)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8575..8808
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8886..9007
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8923..8942)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8977..8996
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9047..9182
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9202..9221)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9203..9221)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9231..9248)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9231..9247)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9240..9262)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9389..9844
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9476..9495)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9686..9707
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9870..9974
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9975..10043,10044..10835)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10193..10212)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11006..11594
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11495..11514
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11748..11765
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11882..11903
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11918..11923,11924..11941,11942..11948)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11956..11972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11961..11983
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11980..11996
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11980..11996
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12015..12035
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12017..12035
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaga tccaaaggca gggcaaggaa
     4141 actggccaca aataatgagt gtgtatatgg caactaccct gaaatacctt tggaagaaat
     4201 gccagatgca gatggagtag ccagcactcc ctccctcaat attcaagagc catgctctcc
     4261 tgccacatcc agtgaagcat tcactccaaa ggagggttct ccttacaaag cccccatcta
     4321 catccctgat gatatcccca ttcctgctga gtttgaactt cgagagtcaa atatgcctgg
     4381 ggcaggacta ggaatatgga ccaaaaggaa gatcgaagta ggtgaaaagt ttgggcctta
     4441 tgtgggagag cagaggtcaa acctgaaaga ccccagttat ggatgggaga tcttagacga
     4501 attttacaat gtgaagttct gcatagatgc cagtcaacca gatgttggaa gctggctcaa
     4561 gtacattaga ttcgctggct gttatgatca gcacaacctt gttgcatgcc agataaatga
     4621 tcagatattc tatagagtag ttgcagacat tgcgccggga gaggagcttc tgctgttcat
     4681 gaagagcgaa gactatcccc atgaaactat ggcgccggat atccacgaag aacggcaata
     4741 tcgctgcgaa gactgtgacc agctctttga atctaaggct gaactagcag atcaccaaaa
     4801 gtttccatgc agtactcctc actcagcatt ttcaatggtt gaagaggact ttcagcaaaa
     4861 actcgaaagc gagaatgatc tccaagagat acacacgatc caggagtgta aggaatgtga
     4921 ccaagttttt cctgatttgc aaagcctgga gaaacacatg ctgtcacata ctgaagagag
     4981 ggaatacaag tgtgatcagt gtcccaaggc atttaactgg aagtccaatt taattcgcca
     5041 ccagatgtca catgacagtg gaaagcacta tgaatgtgaa aactgtgcca aggttttcac
     5101 ggaccctagc aaccttcagc ggcacattcg ctctcagcat gtcggtgccc gggcccatgc
     5161 atgcccggag tgtggcaaaa cgtttgccac ttcgtcgggc ctcaaacaac acaagcacat
     5221 ccacagcagt gtgaagccct ttatctgtga ggtctgccat aaatcctata ctcagttttc
     5281 aaacctttgc cgtcataagc gcatgcatgc tgattgcaga acccaaatca agtgcaaaga
     5341 ctgtggacaa atgttcagca ctacgtcttc cttaaataaa cacaggaggt tttgtgaggg
     5401 caagaaccat tttgcggcag gtggattttt tggccaaggc atttcacttc ctggaacccc
     5461 agctatggat aaaacgtcca tggttaatat gagtcatgcc aacccgggcc ttgctgacta
     5521 ttttggcgcc aataggcatc ctgctggtct tacctttcca acagctcctg gattttcttt
     5581 tagcttccct ggtctgtttc cttccggctt gtaccacagg cctcctttga tacctgctag
     5641 ttctcctgtt aaaggactat caagtactga acagacaaac aaaagtcaaa gtcccctcat
     5701 gacacatcct cagatactgc cagctacaca ggatattttg aaggcactat ctaaacaccc
     5761 atctgtaggg gacaataagc cagtggagct ccagcccgag aggtcctctg aagagaggcc
     5821 ctttgagaaa atcagtgacc agtcagagag tagtgacctt gatgatgtca gtacaccaag
     5881 tggcagtgac ctggaaacaa cctcgggctc tgatctggaa agtgacattg aaagtgataa
     5941 agagaaattt aaagaaaatg gtaaaatgtt caaagacaaa gtaagccctc ttcagaatct
     6001 ggcttcaata aataataaga aagaatacag caatcattcc attttctcac catctttaga
     6061 ggagcagact gcggtgtcag gagctgtgaa tgattctata aaggctattg cttctattgc
     6121 tgaaaaatac tttggttcaa caggactggt ggggctgcaa gacaaaaaag ttggagcttt
     6181 accttaccct tccatgtttc ccctcccatt ttttccagca ttctctcaat caatgtaccc
     6241 atttcctgat agagacttga gatcgttacc tttgaaaatg gaaccccaat caccaggtga
     6301 agtaaagaaa ctgcagaagg gcagctctga gtcccccttt gatctcacca ctaagcgaaa
     6361 ggatgagaag cccttgactc cagtcccctc caagcctcca gtgacacctg ccacaagcca
     6421 agaccagccc ctggatctaa gtatgggcag taggagtaga gccagtggga caaagctgac
     6481 tgagcctcga aaaaaccacg tgtttggggg aaaaaaagga agcaacgtcg aatcaagacc
     6541 tgcttcagat ggttccttgc agcatgcaag acccactcct ttctttatgg accctattta
     6601 cagagtagag aaaagaaaac taactgaccc acttgaagct ttaaaagaga aatacttgag
     6661 gccttctcca ggattcttgt ttcacccaca aatgtcagct attgaaaaca tggcagaaaa
     6721 gctagagagc ttcagtgccc tgaaacctga ggccagtgag ctcttacagt cagtgccctc
     6781 tatgttcaac ttcagggcgc ctcccaatgc cctgccagag aaccttctgc ggaagggaaa
     6841 ggagcgctat acctgcagat actgtggcaa gatttttcca aggtctgcaa acctaacacg
     6901 gcacttgaga acccacacag gagagcagcc ttacagatgc aaatactgtg acagatcatt
     6961 tagcatatct tctaacttgc aaaggcatgt tcgcaacatc cacaataaag agaagccatt
     7021 taagtgtcac ttatgtgata ggtgttttgg tcaacaaacc aatttagaca gacacctaaa
     7081 gaaacatgag aatgggaaca tgtccggtac agcaacatcg tcgcctcatt ctgaactgga
     7141 aagtacaggt gcgattctgg atgacaaaga agatgcttac ttcacagaaa ttcgaaattt
     7201 cattgggaac agcaaccatg gcagccaatc tcccaggaat gtggaggaga gaatgaatgg
     7261 cagtcatttt aaagatgaaa aggctttggt gaccagtcaa aattcagact tgctggatga
     7321 tgaagaagtt gaagatgagg tgttgttaga tgaggaggat gaagacaatg atattactgg
     7381 aaaaacagga aaggaaccag tgacaagtaa tttacatgaa ggaaaccctg aggatgacta
     7441 tgaagaaacc agtgccctgg agatgagttg caagacatcc ccagtgaggt ataaagagga
     7501 agaatataaa agtggacttt ctgctctaga tcatataagg cacttcacag atagcctcaa
     7561 aatgaggaaa atggaagata atcaatattc tgaagctgag ctgtcttctt ttagtacttc
     7621 ccatgtgcca gaggaactta agcagccgtt acacagaaag tccaaatcgc aggcatatgc
     7681 tatgatgctg tcactgtctg acaaggagtc cctccattct acatcccaca gttcttccaa
     7741 cgtgtggcac agtatggcca gggctgcggc ggaatccagt gctatccagt ccataagcca
     7801 cgtatagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     7861 ttgtggaaag gacgagcccg ggctctcaat gtacgtcaaa cgcgttaagt cgacaatcaa
     7921 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7981 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     8041 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     8101 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     8161 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     8221 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     8281 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     8341 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     8401 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     8461 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     8521 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     8581 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     8641 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8701 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8761 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     8821 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     8881 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8941 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     9001 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     9061 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     9121 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     9181 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     9241 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     9301 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     9361 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     9421 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     9481 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     9541 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     9601 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     9661 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     9721 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     9781 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     9841 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     9901 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     9961 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    10021 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    10081 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    10141 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    10201 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
    10261 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    10321 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    10381 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    10441 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    10501 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    10561 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    10621 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    10681 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    10741 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    10801 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    10861 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    10921 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    10981 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    11041 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    11101 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    11161 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    11221 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    11281 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    11341 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    11401 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    11461 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    11521 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    11581 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    11641 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    11701 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    11761 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    11821 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    11881 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    11941 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    12001 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagc
//
