LOCUS       sequence-400380-       12211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12211)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2137
FEATURES             Location/Qualifiers
     source          1..12211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8068..8656
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8121..8141)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8539..8550)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8728..8961
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9039..9160
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9076..9095)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9130..9149
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9200..9335
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9355..9374)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9356..9374)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9384..9401)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9384..9400)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9393..9415)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9542..9997
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9629..9648)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9839..9860
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10023..10127
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10128..10196,10197..10988)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10346..10365)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11159..11747
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11648..11667
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11901..11918
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12035..12056
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12071..12076,12077..12094,12095..12101)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12109..12125
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12114..12136
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12133..12149
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12133..12149
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12168..12188
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12170..12188
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggtc cggaaaaaga acccccctct
     4141 gagaaacgtt gcaagtgaag gcgagggcca gatcctggag cctataggta cagaaagcaa
     4201 ggtatctgga aagaacaaag aattttctgc agatcagatg tcagaaaata cggatcagag
     4261 tgatgctgca gaactaaatc ataaggagga acatagcttg catgttcaag atccatcttc
     4321 tagcagtaag aaggacttga aaagcgcagt tctgagtgag aaggctggct tcaattatga
     4381 aagccccagt aagggaggaa actttccctc ctttccgcat gatgaggtga cagacagaaa
     4441 tatgttggct ttctcatctc cagctgctgg gggagtctgt gagcccttga agtctccgca
     4501 aagagcagag gcagatgacc ctcaagatat ggcctgcacc ccctcagggg actcactgga
     4561 gacaaaggaa gatcagaaga tgtcaccaaa ggctacagag gaaacagggc aagcacagag
     4621 tggtcaagcc aattgtcaag gtttgagccc agtttcagtg gcctcaaaaa acccacaagt
     4681 gccttcagat gggggtgtaa gactgaataa atccaaaact gacttactgg tgaatgacaa
     4741 cccagacccg gcacctctgt ctccagagct tcaggacttt aaatgcaata tctgtggata
     4801 tggttactac ggcaacgacc ccacagatct gattaagcac ttccgaaagt atcacttagg
     4861 actgcataac cgcaccaggc aagatgctga gctggacagc aaaatcttgg cccttcataa
     4921 catggtgcag ttcagccatt ccaaagactt ccagaaggtc aaccgttctg tgttttctgg
     4981 tgtgctgcag gacatcaatt cttcaaggcc tgttttacta aatgggacct atgatgtgca
     5041 ggtgacttca ggtggaacat tcattggcat tggacggaaa acaccagatt gccaagggaa
     5101 caccaagtat ttccgctgta aattctgcaa tttcacttat atgggcaact catccaccga
     5161 attagaacaa cattttcttc agactcaccc aaacaaaata aaagcttctc tcccctcctc
     5221 tgaggttgca aaaccttcag agaaaaactc taacaagtcc atccctgcac ttcaatccag
     5281 tgattctgga gacttgggaa aatggcagga caagataaca gtcaaagcag gagatgacac
     5341 tcctgttggg tactcagtgc ccataaagcc cctcgattcc tctagacaaa atggtacaga
     5401 ggccaccagt tactactggt gtaaattttg tagtttcagc tgtgagtcat ctagctcact
     5461 taaactgcta gaacattatg gcaagcagca cggagcagtg cagtcaggcg gccttaatcc
     5521 agagttaaat gataagcttt ccaggggctc tgtcattaat cagaatgatc tagccaaaag
     5581 ttcagaagga gagacaatga ccaagacaga caagagctcg agtggggcta aaaagaagga
     5641 cttctccagc aagggagccg aggataatat ggtaacgagc tataattgtc agttctgtga
     5701 cttccgatat tccaaaagcc atggccctga tgtaattgta gtggggccac ttctccgtca
     5761 ttatcaacag ctccataaca ttcacaagtg taccattaaa cactgtccat tctgtcccag
     5821 aggactttgc agcccagaaa agcaccttgg agaaattact tatccgtttg cttgtagaaa
     5881 aagtaattgt tcccactgtg cactcttgct tctgcacttg tctcctgggg cggctggaag
     5941 ctcgcgagtc aaacatcagt gccatcagtg ttcattcacc acccctgacg tagatgtact
     6001 cctctttcac tatgaaagtg tgcatgagtc ccaagcatcg gatgtcaaac aagaagcaaa
     6061 tcacctgcaa ggatcggatg ggcagcagtc tgtcaaggaa agcaaagaac actcatgtac
     6121 caaatgtgat tttattaccc aagtggaaga agagatttcc cgacactaca ggagagcaca
     6181 cagctgctac aaatgccgtc agtgcagttt tacagctgcc gatactcagt cactactgga
     6241 gcacttcaac actgttcact gccaggaaca ggacatcact acagccaacg gcgaagagga
     6301 cggtcatgcc atatccacca tcaaagagga gcccaaaatt gacttcaggg tctacaatct
     6361 gctaactcca gactctaaaa tgggagagcc agtttctgag agtgtggtga agagagagaa
     6421 gctggaagag aaggacgggc tcaaagagaa agtttggacc gagagttcca gtgatgacct
     6481 tcgcaatgtg acttggagag gggcagacat cctgcggggg agtccgtcat acacccaagc
     6541 aagcctgggg ctgctgacgc ctgtgtctgg cacccaagag cagacaaaga ctctaaggga
     6601 tagtcccaat gtggaggccg cccatctggc gcgacctatt tatggcttgg ctgtggaaac
     6661 caagggattc ctgcaggggg cgccagctgg cggagagaag tctggggccc tcccccagca
     6721 gtatcctgca tcgggagaaa acaagtccaa ggatgaatcc cagtccctgt tacggaggcg
     6781 tagaggctcc ggtgtttttt gtgccaattg cctgaccaca aagacctctc tctggcgaaa
     6841 gaatgcaaat ggcggatatg tatgcaacgc gtgtggcctc taccagaagc ttcactcgac
     6901 tcccaggcct ttaaacatca ttaaacaaaa caacggtgag cagattatta ggaggagaac
     6961 aagaaagcgc cttaacccag aggcacttca ggctgagcag ctcaacaaac agcagagggg
     7021 cagcaatgag gagcaagtca atggaagccc gttagagagg aggtcagaag atcatctaac
     7081 tgaaagtcac cagagagaaa ttccactccc cagcctaagt aaatacgaag cccagggttc
     7141 attgactaaa agccattctg ctcagcagcc agtcctggtc agccaaactc tggatattca
     7201 caaaaggatg caacctttgc acattcagat aaaaagtcct caggaaagta ctggagatcc
     7261 aggaaatagt tcatccgtat ctgaagggaa aggaagttct gagagaggca gtcctataga
     7321 aaagtacatg agacctgcga aacacccaaa ttattcacca ccaggcagcc ctattgaaaa
     7381 gtaccagtac ccactttttg gacttccctt tgtacataat gacttccaga gtgaagctga
     7441 ttggctgcgg ttctggagta aatataagct ctccgttcct gggaatccgc actacttgag
     7501 tcacgtgcct ggcctaccaa atccttgcca aaactatgtg ccttatccca ccttcaatct
     7561 gcctcctcat ttttcagctg ttggatcaga caatgacatt cctctagatt tggcgatcaa
     7621 gcattccaga cctgggccaa ctgcaaacgg tgcctccaag gagaaaacga aggcaccacc
     7681 aaatgtaaaa aatgaaggtc ccttgaatgt agtaaaaaca gagaaagttg atagaagtac
     7741 tcaagatgaa ctttcaacaa aatgtgtgca ctgtggcatt gtctttctgg atgaagtgat
     7801 gtatgctttg catatgagtt gccatggtga cagtggacct ttccagtgca gcatatgcca
     7861 gcatctttgc acggacaaat atgacttcac aacacatatc cagaggggcc tgcataggaa
     7921 caatgcacaa gtggaaaaaa atggaaaacc taaagagtag taatgaacta gtccgtaact
     7981 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgagg gatctgtgtt
     8041 gctgtacaga gaacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     8101 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     8161 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     8221 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     8281 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     8341 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     8401 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     8461 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     8521 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     8581 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     8641 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     8701 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     8761 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8821 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8881 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8941 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     9001 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     9061 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     9121 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     9181 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     9241 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     9301 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     9361 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     9421 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     9481 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     9541 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     9601 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     9661 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     9721 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     9781 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     9841 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     9901 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     9961 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
    10021 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
    10081 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
    10141 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
    10201 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
    10261 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
    10321 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
    10381 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    10441 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    10501 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
    10561 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    10621 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
    10681 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    10741 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    10801 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    10861 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    10921 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    10981 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    11041 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    11101 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    11161 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    11221 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    11281 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    11341 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    11401 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    11461 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    11521 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    11581 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    11641 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    11701 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    11761 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    11821 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    11881 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    11941 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    12001 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    12061 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    12121 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
    12181 actaaaggga acaaaagctg gagctgcaag c
//
