LOCUS       sequence-400407-       12040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12040)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1646
FEATURES             Location/Qualifiers
     source          1..12040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7897..8485
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7950..7970)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8368..8379)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8557..8790
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8868..8989
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8905..8924)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8959..8978
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9029..9164
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9184..9203)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9185..9203)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9213..9230)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9213..9229)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9222..9244)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9371..9826
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9458..9477)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9668..9689
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9852..9956
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9957..10025,10026..10817)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10175..10194)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10988..11576
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11477..11496
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11730..11747
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11864..11885
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11900..11905,11906..11923,11924..11930)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11938..11954
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11943..11965
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11962..11978
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11962..11978
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11997..12017
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11999..12017
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag gatgaatcaa tttacacctg
     4141 cgatcactgt cagcaggact tcgagtctct ggcagacctg acggaccacc gggcccaccg
     4201 ctgtcctgga gatggtgatg acgacccaca actctcctgg gtggcctcgt ctccctccag
     4261 caaggatgtt gcgtcaccca cgcagatgat cggagatggt tgtgacctcg gcctcggcga
     4321 ggaggaaggg ggcacgggcc tgccataccc ttgccagttc tgcgacaagt ccttcatccg
     4381 cttgagctac ttgaagaggc acgagcagat ccacagcgac aagctgccgt tcaagtgcac
     4441 ctactgcagc cgcctcttca agcacaagag gagccgtgac cggcacatca agctgcatac
     4501 gggcgacaag aagtatcact gccacgagtg cgaggcagcc ttctcccgca gcgaccacct
     4561 caagatccac ctgaagaccc acagctccag caagcccttc aagtgcactg tgtgcaagcg
     4621 cggcttctcc tccaccagct cgctgcagag ccacatgcag gcccacaaaa agaacaagga
     4681 gcatctggcc aagtcggaga aggaagccaa gaaggacgac ttcatgtgcg actactgcga
     4741 ggacaccttc agccagacgg aggagctgga gaagcacgtg ctcacccgcc acccgcagct
     4801 gtccgagaag gcggacctgc agtgcattca ctgccctgag gtcttcgtcg acgagaacac
     4861 actgctcgcc catatccacc aagcccacgc caaccagaaa cacaagtgcc ccatgtgccc
     4921 tgagcagttc tcctcagtgg aaggtgtcta ctgccacctg gacagccacc ggcagcccga
     4981 ctccagcaac cacagtgtca gtcccgaccc tgtactgggc agcgtggcct ccatgagcag
     5041 cgccacaccc gactccagcg cctctgtgga gcgtggctcc accccggact ccaccttgaa
     5101 gccgctgcgg gggcagaaga agatgcggga tgacgggcag ggctggacca aggtggtcta
     5161 tagctgcccc tattgttcca agcgggactt taacagcctg gccgtgctgg agatccacct
     5221 gaagaccatc cacgcggaca agccccagca gagccacaca tgtcagatct gcctggactc
     5281 catgcccacc ctctacaacc tcaacgagca cgttcgcaag ctgcacaaga accatgccta
     5341 ccctgtgatg cagtttggca acatctctgc cttccactgc aactactgcc ccgagatgtt
     5401 cgccgacatc aatagcctgc aggagcacat ccgcgtctcc cactgcggcc ccaacgccaa
     5461 cccctctgac ggtaataatg ctttcttctg caaccagtgc tccatgggtt tccttactga
     5521 gtcctccctc accgagcaca tccagcaggc ccactgcagt gtgggcagtg ccaaactaga
     5581 gtctccggtg gtgcagccca cgcagtcctt catggaggtc tattcctgcc cctactgcac
     5641 caactccccc atctttggct ccatcctgaa actcaccaag cacatcaagg agaaccacaa
     5701 gaacattcca ctggcccaca gcaagaagtc caaggccgag cagagcccag tctcgtccga
     5761 tgtggaggtg tcttccccga agcggcagcg gctctcagca agcgccaact ccatctccaa
     5821 tggggagtat ccttgcaatc aatgcgacct caagttctcc aactttgaga gcttccagac
     5881 ccacctgaag ctgcacctgg agctgctgct gcggaagcaa gcgtgccccc agtgcaaaga
     5941 ggactttgac tcccaggagt ccctcctgca gcacctgaca gtgcattaca tgaccacgtc
     6001 gacccactat gtgtgcgaga gctgcgacaa gcaattttcc tcggtggatg acctgcagaa
     6061 gcacctgctg gacatgcaca cctttgtgtt gtaccactgc accctgtgtc aggaggtctt
     6121 cgactccaag gtgtccatcc aggtgcacct ggcggtgaag cacagcaatg agaagaagat
     6181 gtaccgctgc acggcctgca actgggactt ccgcaaggag gctgacctgc aggtgcacgt
     6241 caaacacagc cacctgggca acccggccaa ggctcacaag tgcatcttct gtggggagac
     6301 cttcagcacc gaggtggagc tgcagtgcca catcaccaca cacagcaaga agtataactg
     6361 taagttctgc agcaaggcct tccacgccat catcctgctg gagaagcacc tgcgggagaa
     6421 gcactgtgtg tttgatgctg cgaccgagaa cggcacggcc aatggggtac ccccaatggc
     6481 caccaagaaa gctgagcctg ctgacctgca gggcatgctg cttaagaacc ctgaggcacc
     6541 taacagccat gaggccagcg aggatgacgt ggacgcgtcg gagcccatgt acggctgtga
     6601 catctgtggg gcggcctaca ccatggaggt gctgctgcag aatcaccggc tgcgggacca
     6661 caatatccgg ccgggcgagg atgatggctc acgcaagaag gctgagttta tcaagggcag
     6721 tcacaagtgc aacgtttgtt cacggacttt cttctcggag aacgggctac gggagcacct
     6781 gcagacgcac cggggccctg ccaagcacta catgtgtccc atctgtggtg agcgcttccc
     6841 ttcgctgctg acgctcaccg aacacaaggt gacccacagc aagagcctgg acacgggcac
     6901 ctgtcgcatc tgcaagatgc ccctgcagag cgaggaggag tttattgagc actgccagat
     6961 gcaccctgac ctgcgcaact cactcacggg cttccgctgt gtggtctgca tgcagacagt
     7021 cacttccacg cttgagctca agatccatgg caccttccac atgcagaagc tggcgggcag
     7081 ctcagcggcg tcctccccca atggccaggg gctgcagaag ctctacaagt gcgccctgtg
     7141 cctcaaggag ttccgcagca agcaggacct ggtgaagctt gacgtcaatg ggctgcccta
     7201 cggcctctgc gccggctgca tggcccgcag cgccaacgga caggtgggtg gcctggcccc
     7261 gcccgagccc gccgaccggc cctgtgccgg cctccgttgc cccgagtgca gtgtcaagtt
     7321 tgagagtgcc gaagacctgg agagccacat gcaggtggac caccgtgacc tcacgccgga
     7381 gaccagtggg ccccggaaag gcacccagac atcgccagtg ccccggaaaa agacatacca
     7441 gtgcatcaag tgccagatga ccttcgagaa cgagagagag atccaaatcc acgttgccaa
     7501 ccacatgatt gaggaaggca tcaaccacga gtgtaagctg tgcaaccaga tgttcgactc
     7561 cccggccaag ctcctctgtc acctcattga gcacagcttc gagggcatgg gcggcacctt
     7621 caaatgcccc gtgtgtttca cagtcttcgt ccaggccaac aagttgcagc agcacatctt
     7681 tgccgtgcac gggcaggagg acaagatcta cgactgctca cagtgccctc agaagttctt
     7741 cttccagacc gagctgcaga accacacgat gagccagcac gcacagtagt aatgaactag
     7801 tccgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaagt
     7861 tgcccagacg attgggcgtg cacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     7921 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     7981 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     8041 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     8101 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     8161 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     8221 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     8281 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     8341 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     8401 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     8461 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     8521 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     8581 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     8641 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8701 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8761 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     8821 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     8881 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     8941 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     9001 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     9061 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     9121 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     9181 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     9241 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     9301 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     9361 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     9421 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     9481 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     9541 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     9601 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     9661 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     9721 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     9781 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     9841 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     9901 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     9961 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
    10021 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
    10081 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
    10141 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
    10201 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
    10261 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
    10321 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
    10381 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
    10441 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
    10501 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
    10561 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
    10621 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
    10681 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
    10741 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    10801 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    10861 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    10921 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    10981 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    11041 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    11101 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
    11161 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    11221 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    11281 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    11341 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    11401 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    11461 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    11521 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    11581 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    11641 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    11701 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    11761 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
    11821 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
    11881 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
    11941 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
    12001 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
//
