LOCUS       sequence-400408-       12079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12079)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1572
FEATURES             Location/Qualifiers
     source          1..12079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7936..8524
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7989..8009)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8407..8418)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8596..8829
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8907..9028
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8944..8963)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8998..9017
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9068..9203
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9223..9242)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9224..9242)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9252..9269)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9252..9268)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9261..9283)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9410..9865
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9497..9516)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9707..9728
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9891..9995
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9996..10064,10065..10856)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10214..10233)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11027..11615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11516..11535
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11769..11786
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11903..11924
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11939..11944,11945..11962,11963..11969)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11977..11993
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11982..12004
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12001..12017
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12001..12017
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12036..12056
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12038..12056
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgggg gatatgaaga cccctgattt
     4141 tgatgacctc cttgctgcct ttgacatccc tgacattgat gcgaatgaag ccatccattc
     4201 tgggccagaa gaaaatgagg ggcctggagg cccagggaag ccagaaccag gtgtaggaag
     4261 tgaatctgaa gacacagcag cagcctctgc tggggatggc cctggagttc cagcccaggc
     4321 ctctgaccat ggcctgccac cgccagacat ttctgtagtc agtgtcattg tcaagaacac
     4381 tgtgtgtccc gagcagtctg aggccctggc tggaggctca gcaggagacg gggcccaggc
     4441 tgctggggta actaaagaag ggcctgtggg gcctcatcga atgcagaatg gttttgggag
     4501 ccctgaacct tccctcccag gaactcccca ctctcctgct cctcccagtg ggggcacctg
     4561 gaaagaaaaa ggcatggaag gcaaaactcc cttggacctg tttgctcatt ttggccctga
     4621 gccaggggac cactcagatc cgctgcctcc ctctgcaccc tctcccactc gggagggggc
     4681 tctgaccccg cctcctttcc cctcttcctt tgagctggcc caggagaatg gcccaggcat
     4741 gcagccacct gtttcttccc caccattggg ggccttgaag caggagagct gcagccccca
     4801 tcatccccag gtcctagccc aacaaggctc aggctccagc cctaaggcca cggacatccc
     4861 tgccagtgcc tcgcctcccc cagttgctgg ggtgcccttc ttcaagcagt ctccagggca
     4921 ccagagccct cttgcctccc ccaaagtgcc cgtctgtcag cccttgaagg aagaagatga
     4981 tgatgagggg ccagtggaca agtcttcccc aggaagtccc cagagtccct ctagtggggc
     5041 cgaggctgca gatgaggaca gcaatgactc ccctgcctcc agctcctcta ggcctcttaa
     5101 ggtgcggatc aagaccatta aaacatcctg cgggaatatc acaaggactg taactcaggt
     5161 cccctcagat cctgatccac ctgccccctt ggctgagggg gccttcttgg ctgaggctag
     5221 cctcttgaag ctgtcccctg caacacctac ttctgagggt ccaaaggtgg tgagcgtaca
     5281 gttgggtgat ggtacaaggc tgaaaggcac tgtgctgcct gtggccacca tccagaacgc
     5341 cagtactgcc atgctgatgg cagccagtgt ggctcgcaag gctgtggtgc tgcctggggg
     5401 gactgccacc agccctaaga tgattgctaa gaacgtgcta ggcctggtgc cccaagccct
     5461 gcctaaggct gacgggcggg cagggctggg gactggggga cagaaggtga atggtgcctc
     5521 ggtggtgatg gtgcaacctt caaagacagc tactgggcca agtacagggg gcggcacagt
     5581 gatatcacgg acccagtcca gcctggtgga ggccttcaac aagatcctca acagcaagaa
     5641 cctgctccct gcctataggc caaacctgag cccaccagct gaggctgggc tggccctgcc
     5701 tcccaccggc taccgctgcc tggagtgtgg ggatgccttc tcattggaga agagcctggc
     5761 acggcactat gaccgtcgga gcatgcgcat cgaggtcacc tgcaaccact gcgcccgccg
     5821 cctggtcttc ttcaacaagt gcagcctgct cctgcatgca cgtgaacaca aggacaaggg
     5881 gctcgtcatg cagtgctcac atttggtcat gaggcctgta gcccttgacc agatggtggg
     5941 gcagccggac atcacaccgc tgctgcctgt agctgtccca cctgtctctg gacctctggc
     6001 cttgcctgcc ttgggcaagg gtgagggggc catcacctcc tctgccatta ctacagttgc
     6061 tgctgaggcc cctgtcctgc cgctctccac agagccgcct gctgccccgg ccacctctgc
     6121 ttacacatgc tttcgctgcc tggagtgcaa ggaacagtgc cgggacaagg ctggcatggc
     6181 agctcacttc cagcagctcg gcccccctgc ccctggggcc accagcaatg tgtgcccaac
     6241 ctgccccatg atgctcccca atcgctgcag cttcagcgcc caccagcgca tgcataagaa
     6301 tcgacccccc catgtctgtc ctgagtgtgg gggcaacttc ctgcaagcca attttcagac
     6361 ccatctccgg gaggcctgtc tgcacgtctc tcgccgtgta ggatacaggt gccccagctg
     6421 ttcagtggtg tttgggggtg tgaactccat caagtcccac atccagacgt cgcactgcga
     6481 ggttttccac aagtgcccca tctgccccat ggccttcaag tctgggccaa gtgcccatgc
     6541 ccacctctac tcccagcatc ccagcttcca aactcagcag gccaagctga tctacaagtg
     6601 cgccatgtgc gacacagtct tcactcacaa acccctcctc tcctcacact tcgaccagca
     6661 cttgctgccc cagcgtgtca gtgtctttaa gtgcccgtct tgtcctctgc tctttgccca
     6721 aaaaaggacc atgctggaac atctcaagaa cacccatcag tctgggcgct tggaggagac
     6781 tgctgggaaa ggggccgggg gtgccctgct gacccccaag actgagcctg aggagctggc
     6841 tgtttctcag ggaggggcag cccctgctac tgaggagtcg tcttcatctt cagaagagga
     6901 ggaagtaccc agctcccctg agcccccccg tccagccaaa cggcctcggc gggaactagg
     6961 gagcaaaggc ctcaagggtg ggggtggggg gcctggaggc tggacctgtg gcctgtgtca
     7021 ctcctggttc cctgagcgtg atgaatacgt ggcccacatg aagaaggagc atggcaagtc
     7081 agtgaaaaag ttcccctgtc gcctgtgtga gcgctccttc tgctccgccc ccagcctgag
     7141 gcgccatgtc agagttaatc acgagggcat caagcgagtt tacccctgca ggtattgcac
     7201 agagggaaaa cgcaccttca gcagccgcct gatcctagag aaacatgtcc aggtccggca
     7261 cggcttgcag cttggggccc agtcccctgg ccgggggacc accttggctc ggggttccag
     7321 tgccagagcc caggggccag gtcggaaacg ccgccagtct tctgactctt gcagtgagga
     7381 gcctgacagc acgacaccgc cagccaagtc ccccaggggc ggacctggat ctggaggcca
     7441 tggccctctg cgctaccgga gcagcagctc cacagaacag agcctcatga tggggttgag
     7501 ggtggaggat ggtgcccagc agtgcctcga ctgtggcttg tgctttgcct cccctggctc
     7561 cctgagccga caccgtttca tcagccacaa gaagagacgg ggtgtgggta aagccagtgc
     7621 cctggggctg ggggatgggg aggaagaggc ccctccatca aggtctgacc ccgatggtgg
     7681 agactcaccc ctgcctgctt ctggaggccc actgacctgt aaggtctgtg gcaagagctg
     7741 cgacagccct ctaaacctca agacccactt ccgcacgcat ggcatggcgt tcatcagggc
     7801 tcggcagggg gctgttgggg acaactagta atgaactagt ccgtaacttg aaagtatttc
     7861 gatttcttgg ctttatatat cttgtggaaa ggacgaagcg ggaggagtaa cgtagcgcag
     7921 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     7981 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     8041 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     8101 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     8161 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     8221 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     8281 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     8341 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     8401 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     8461 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     8521 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     8581 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     8641 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8701 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8761 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8821 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     8881 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     8941 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9001 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     9061 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9121 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     9181 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     9241 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     9301 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     9361 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     9421 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     9481 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     9541 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     9601 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     9661 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     9721 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     9781 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     9841 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     9901 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     9961 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    10021 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    10081 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
    10141 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
    10201 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
    10261 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    10321 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    10381 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    10441 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    10501 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    10561 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    10621 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    10681 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    10741 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    10801 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    10861 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10921 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    10981 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    11041 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    11101 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    11161 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    11221 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    11281 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    11341 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    11401 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    11461 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    11521 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    11581 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    11641 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    11701 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    11761 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    11821 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    11881 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    11941 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    12001 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
    12061 aaaagctgga gctgcaagc
//
