LOCUS       sequence-400413-       10657 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10657)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1511
FEATURES             Location/Qualifiers
     source          1..10657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6514..7102
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6567..6587)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6985..6996)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7174..7407
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7485..7606
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7522..7541)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7576..7595
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7646..7781
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7801..7820)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7802..7820)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7830..7847)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7830..7846)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7839..7861)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7988..8443
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8075..8094)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8285..8306
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8469..8573
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8574..8642,8643..9434)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8792..8811)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9605..10193
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10094..10113
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10347..10364
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10481..10502
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10517..10522,10523..10540,10541..10547)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10555..10571
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10560..10582
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10579..10595
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10579..10595
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10614..10634
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10616..10634
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcg ggctggatcc aggcccagca
     4141 gctgcaggga gacgcgctgc gccagatgca ggtgctgtac ggccagcact tccccatcga
     4201 ggtccggcac tacttggccc agtggattga gagccagcca tgggatgcca ttgacttgga
     4261 caatccccag gacagagccc aagccaccca gctcctggag ggcctggtgc aggagctgca
     4321 gaagaaggcg gagcaccagg tgggggaaga tgggttttta ctgaagatca agctggggca
     4381 ctacgccacg cagctccaga aaacatatga ccgctgcccc ctggagctgg tccgctgcat
     4441 ccggcacatt ctgtacaatg aacagaggct ggtccgagaa gccaacaatt gcagctctcc
     4501 ggctgggatc ctggttgacg ccatgtccca gaagcacctt cagatcaacc agacatttga
     4561 ggagctgcga ctggtcacgc aggacacaga gaatgagctg aagaaactgc agcagactca
     4621 ggagtacttc atcatccagt accaggagag cctgaggatc caagctcagt ttgcccagct
     4681 ggcccagctg agcccccagg agcgtctgag ccgggagacg gccctccagc agaagcaggt
     4741 gtctctggag gcctggttgc agcgtgaggc acagacactg cagcagtacc gcgtggagct
     4801 ggccgagaag caccagaaga ccctgcagct gctgcggaag cagcagacca tcatcctgga
     4861 tgacgagctg atccagtgga agcggcggca gcagctggcc gggaacggcg ggccccccga
     4921 gggcagcctg gacgtgctac agtcctggtg tgagaagttg gccgagatca tctggcagaa
     4981 ccggcagcag atccgcaggg ctgagcacct ctgccagcag ctgcccatcc ccggcccagt
     5041 ggaggagatg ctggccgagg tcaacgccac catcacggac attatctcag ccctggtgac
     5101 cagcacattc atcattgaga agcagcctcc tcaggtcctg aagacccaga ccaagtttgc
     5161 agccaccgta cgcctgctgg tgggcgggaa gctgaacgtg cacatgaatc ccccccaggt
     5221 gaaggccacc atcatcagtg agcagcaggc caagtctctg cttaaaaatg agaacacccg
     5281 caacgagtgc agtggtgaga tcctgaacaa ctgctgcgtg atggagtacc accaagccac
     5341 gggcaccctc agtgcccact tcaggaacat gtcactgaag aggatcaagc gtgctgaccg
     5401 gcggggtgca gagtccgtga cagaggagaa gttcacagtc ctgtttgagt ctcagttcag
     5461 tgttggcagc aatgagcttg tgttccaggt gaagggcagg gtgccatttg ccgtgcctga
     5521 caaagtgctg tggccgcagc tgtgtgaggc gctcaacatg aaattcaagg ccgaagtgca
     5581 gagcaaccgg ggcctgacca aggagaacct cgtgttcctg gcgcagaaac tgttcaacaa
     5641 cagcagcagc cacctggagg actacagtgg cctgtccgtg tcctggtccc agttcaacag
     5701 ggagaacttg ccgggctgga actacacctt ctggcagtgg tttgacgggg tgatggaggt
     5761 gttgaagaag caccacaagc cccactggaa tgatggggcc atcctaggtt ttgtgaataa
     5821 gcaacaggcc cacgacctgc tcatcaacaa gcccgacggg accttcttgt tgcgctttag
     5881 tgactcagaa atcgggggca tcaccatcgc ctggaagttt gactccccgg aacgcaacct
     5941 gtggaacctg aaaccattca ccacgcggga tttctccatc aggtccctgg ctgaccggct
     6001 gggggacctg agctatctca tctatgtgtt tcctgaccgc cccaaggatg aggtcttctc
     6061 caagtactac actcctgtgc tggctaaagc tgttgatgga tatgtgaaac cacagatcaa
     6121 gcaagtggtc cctgagtttg tgaatgcatc tgcagatgct gggggcagca gcgccacgta
     6181 catggaccag gccccctccc cagctgtgtg cccccaggct ccctataaca tgtacccaca
     6241 gaaccctgac catgtactcg atcaggatgg agaattcgac ctggatgaga ccatggatgt
     6301 ggccaggcac gtggaggaac tcttacgccg accaatggac agtcttgact cccgcctctc
     6361 gccccctgcc ggtcttttca cctctgccag aggctccctc tcatagtaat gaactagtcc
     6421 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgatacaag
     6481 gcacacgtgt ctcacgtcac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     6541 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6601 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6661 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6721 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6781 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6841 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6901 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     6961 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7021 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7081 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     7141 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     7201 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     7261 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7321 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7381 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     7441 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     7501 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7561 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     7621 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7681 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     7741 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     7801 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     7861 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     7921 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     7981 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     8041 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     8101 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8161 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     8221 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     8281 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     8341 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     8401 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     8461 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     8521 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     8581 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     8641 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     8701 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     8761 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     8821 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     8881 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     8941 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     9001 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     9061 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     9121 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     9181 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     9241 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     9301 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     9361 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     9421 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     9481 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9541 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9601 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9661 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     9721 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     9781 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9841 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9901 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9961 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    10021 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    10081 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    10141 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10201 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    10261 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    10321 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    10381 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    10441 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    10501 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    10561 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
    10621 accctcacta aagggaacaa aagctggagc tgcaagc
//
