LOCUS       sequence-400439-       13000 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13000)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2773
FEATURES             Location/Qualifiers
     source          1..13000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4445..4456
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    8857..9445
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8910..8930)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9328..9339)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9517..9750
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9828..9949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9865..9884)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9919..9938
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9989..10124
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10144..10163)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10145..10163)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10173..10190)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10173..10189)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10182..10204)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10331..10786
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10418..10437)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10628..10649
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10812..10916
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10917..10985,10986..11777)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11135..11154)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11948..12536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12437..12456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12690..12707
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12824..12845
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12860..12865,12866..12883,12884..12890)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12898..12914
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12903..12925
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12922..12938
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12922..12938
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12957..12977
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12959..12977
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag gcggccacca cactgcaccc
     4141 aggcccgcgc ccggcgctgc ccctcggggg cccgggcccg ctgggcgagt tcctgcctcc
     4201 acccgagtgc ccggtcttcg aacccagctg ggaagagttc gcggacccct tcgctttcat
     4261 ccacaagatc cggcccatag ccgagcagac tggcatctgt aaggtgcggc cgccgccgga
     4321 ttggcagcca ccatttgcat gtgatgttga taaacttcat tttacgccac gtatccagag
     4381 actgaatgaa ttggaggccc aaactcgtgt aaaattgaat ttcttggacc agattgcaaa
     4441 gtactgggag ttacagggaa gtactctgaa aattccacat gtggagagga agatcttgga
     4501 cttatttcag cttaataagt tagttgcaga agaaggtgga tttgcagttg tttgcaagga
     4561 tagaaaatgg accaaaattg ctaccaagat ggggtttgct cctggcaaag cagtgggctc
     4621 acatatcaga gggcattatg aacgaattct caacccctac aacttattcc tgtccggaga
     4681 cagcctaagg tgtttgcaga agccaaacct gaccacagac actaaggaca aggagtacaa
     4741 accccatgat attccccaga ggcagtctgt gcagccttcg gaaacgtgcc ccccagcccg
     4801 acgagcaaaa cgcatgagag cagaggccat gaatattaaa atagaacccg aggagacaac
     4861 ggaagccaga actcataatc tgagacgtcg aatgggttgt ccaactccaa aatgtgaaaa
     4921 tgagaaagaa atgaagagta gcatcaagca agaacctatt gagaggaaag attatattgt
     4981 agaaaatgag aaggaaaagc ccaagagtcg atctaaaaaa gccaccaatg ctgtggacct
     5041 gtatgtctgt cttttatgtg gcagtggcaa tgatgaagac cggctactgt tgtgtgatgg
     5101 ctgtgatgac agttaccata ccttttgctt gatcccacct ctccatgatg ttcccaaggg
     5161 agactggagg tgtcctaagt gtttggctca ggaatgtagt aagccacaag aagcatttgg
     5221 ctttgaacaa gcagccaggg actataccct ccgtactttt ggggaaatgg cagatgcgtt
     5281 caaatctgat tacttcaaca tgccagtcca tatggtcccc acagagcttg ttgagaaaga
     5341 attttggaga ctagtaagca ctattgagga ggatgtcaca gtggaatatg gagctgacat
     5401 tgcctcaaag gaatttggca gtggctttcc tgtccgagat gggaaaatca aactctcacc
     5461 tgaggaagag gagtatcttg atagtggctg gaatttgaac aacatgccag tgatggagca
     5521 gtctgtcctt gcacatatta ctgctgatat atgtggcatg aaacttcctt ggttgtatgt
     5581 gggaatgtgc ttttcttcat tctgttggca cattgaagac cactggagct attcaattaa
     5641 ctacttgcac tggggtgagc caaaaacctg gtatggagtc ccagggtatg ctgctgagca
     5701 gctagaaaat gtaatgaaga aactagctcc agaactcttt gtgtcccagc cggatctcct
     5761 ccatcagctt gtgaccatca tgaaccccaa taccctgatg actcatgaag tgcctgttta
     5821 ccgaactaat cagtgtgctg gggagtttgt gattacattt ccaagagcct accacagtgg
     5881 ttttaaccag ggttttaatt ttgctgaggc tgttaacttc tgcactgttg attggctgcc
     5941 attaggccga cagtgtgtgg agcattatcg cttgcttcat cgatattgtg tgttttccca
     6001 cgatgagatg atctgcaaga tggcttccaa ggctgatgta ttagatgttg tagtggcttc
     6061 aactgttcag aaagacatgg ccattatgat tgaggatgag aaagctttaa gagaaactgt
     6121 ccgtaaattg ggagtgattg attcggaaag aatggatttt gagctgttgc cagatgatga
     6181 acgtcagtgt gtaaaatgca aaactacatg cttcatgtct gccatctcct gttcttgtaa
     6241 acctggcctt cttgtttgcc tgcatcatgt aaaagaattg tgttcctgtc ctccttacaa
     6301 atataaattg cggtataggt acacgctgga tgatctctac cctatgatga atgcattgaa
     6361 gcttcgagca gaatcttaca acgaatgggc cttgaatgtg aatgaagctt tggaggcaaa
     6421 gatcaacaag aagaaaagcc ttgtcagctt caaggcttta attgaagaat ctgaaatgaa
     6481 gaaattccca gacaatgatc ttttgcgaca ccttcgccta gtcacacagg atgcagagaa
     6541 gtgtgcctct gttgcgcagc agttgcttaa tggcaaaagg caaactagat atcgatctgg
     6601 tggagggaaa tcccaaaatc agttgacagt gaatgagctc cggcagtttg taacacagct
     6661 gtatgctctt ccatgtgtcc tcagtcagac accattacta aaggatctct tgaatcgtgt
     6721 agaagatttt caacagcata gtcagaaact actctctgag gaaacgccta gtgctgcgga
     6781 gctgcaggac ttgctagatg tcagctttga atttgatgtt gaacttccac agcttgctga
     6841 gatgcgtatc cgtttggaac aagcccgttg gctagaagag gtgcagcaag cttgcctaga
     6901 ccccagctcc cttactttag atgatatgag acgtctcata gacctagggg tagggctggc
     6961 cccgtattca gcagtggaga aagctatggc ccggctgcag gaactgctca cagtgtcaga
     7021 gcactgggac gacaaagcca agagtctcct caaggccagg ccacgacatt cattgaatag
     7081 ccttgctacg gcagtaaagg aaatcgaaga gatccctgca tatctgccca atggtgcggc
     7141 tctgaaagac tcagtgcaga gagccagaga ctggcttcag gatgtagagg gcctgcaggc
     7201 tggaggacgt gtgccagtgt tagacacact catagaactt gttacacgag gccgatctat
     7261 ccccgtacat ctgaattctt tgccaagact ggaaacccta gtagctgagg ttcaggcttg
     7321 gaaagaatgt gctgttaata cattcttgac tgagaattct ccatattctc tcttagaggt
     7381 gctgtgtcct cgatgtgata ttggcctttt gggattgaaa aggaagcaga gaaagttaaa
     7441 ggagcccttg ccaaatggaa agaaaaaaag caccaaatta gagagtctga gtgacctgga
     7501 gagagcttta actgaaagca aggagactgc ttcagctatg gcaactcttg gggaagctcg
     7561 cctaagggaa atggaagcct tgcagtctct cagactcgcc aatgaaggga aattgctgtc
     7621 gcctctccaa gatgtggata taaaaatctg cctatgtcag aaggccccag ctgcccctat
     7681 gattcaatgt gaactctgca gggatgcttt ccacaccagt tgtgtggcgg tacccagtat
     7741 ttcacagggc ctgcgaatct ggctttgtcc ccattgtcgg aggtcagaga aacctccatt
     7801 agagaaaatt ctgcccctgc tcgcctccct tcagcgtatc cgagttcgcc ttcctgaggg
     7861 agatgcactt cgatatatga ttgaaagaac cgtgaactgg cagcacagag cccagcaact
     7921 gctttcgtca gggaatctta aatttgtgca agatcgagtg ggctcaggac tgttatatag
     7981 cagatggcaa gcctcagcag gacaggtgtc agacacaaac aaggtatctc aacctcctgg
     8041 cacaacatca ttttctttgc ctgatgactg ggacaacaga acctcatatt tgcactcccc
     8101 cttctcaact ggacgaagtt gtatccccct ccatggtgtt agtccagaag tgaatgaact
     8161 attgatggaa gcccagctgc tccaggtatc ccttcctgaa attcaggaac tttaccagac
     8221 tttacttgca aagccaagcc ctgctcagca gactgaccga agctcaccag tgagacccag
     8281 cagtgagaag aatgactgtt gccgagggaa gcgagatgga attaacagtc ttgagagaaa
     8341 actgaagaga cgcctggaaa gagagggcct ctccagtgag cggtgggaac gagttaagaa
     8401 aatgcggacc cccaaaaaga agaaaatcaa actgagccac cccaaggaca tgaacaattt
     8461 caagttagag agagagcgta gctatgaatt agttcgttct gctgaaactc attccctgcc
     8521 ctcagacaca tcctattccg aacaggaaga ctctgaggat gaagatgcca tctgcccagc
     8581 tgtgagctgc ctgcagccag aaggagatga ggtggactgg gtccagtgtg atggcagctg
     8641 caatcagtgg tttcatcagg tctgtgttgg tgtctcccca gagatggcag agaaagaaga
     8701 ctacatctgt gtgcgctgta ctgtgaagga cgcaccaagc cgaaagtagt aatgaactag
     8761 tccgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgattg
     8821 ggtaccgggt tctatgctcc gacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     8881 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     8941 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     9001 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     9061 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     9121 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     9181 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     9241 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     9301 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     9361 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     9421 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     9481 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     9541 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     9601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     9661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     9721 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     9781 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     9841 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     9901 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     9961 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
    10021 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    10081 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
    10141 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
    10201 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
    10261 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
    10321 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
    10381 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
    10441 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
    10501 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
    10561 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
    10621 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
    10681 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
    10741 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
    10801 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
    10861 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
    10921 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
    10981 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
    11041 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
    11101 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
    11161 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
    11221 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
    11281 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
    11341 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
    11401 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
    11461 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
    11521 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
    11581 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
    11641 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
    11701 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    11761 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    11821 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    11881 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    11941 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    12001 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    12061 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
    12121 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    12181 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    12241 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    12301 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    12361 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    12421 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    12481 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    12541 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    12601 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    12661 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    12721 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
    12781 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
    12841 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
    12901 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
    12961 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
//
