LOCUS       sequence-400453-       12814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12814)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2769
FEATURES             Location/Qualifiers
     source          1..12814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8671..9259
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8724..8744)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9142..9153)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9331..9564
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9642..9763
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9679..9698)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9733..9752
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9803..9938
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9958..9977)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9959..9977)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9987..10004)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9987..10003)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9996..10018)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10145..10600
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10232..10251)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10442..10463
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10626..10730
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10731..10799,10800..11591)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10949..10968)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11762..12350
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12251..12270
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12504..12521
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12638..12659
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12674..12679,12680..12697,12698..12704)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12712..12728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12717..12739
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12736..12752
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12736..12752
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12771..12791
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12773..12791
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggaa ccggggtgtg acgagttcct
     4141 gccgccaccg gagtgcccgg tttttgagcc tagctgggct gaattccaag acccgcttgg
     4201 ctacattgcg aaaataaggc ccatagcaga gaagtctggc atctgcaaaa tccgcccacc
     4261 cgcggattgg cagcctcctt ttgcagtaga agttgacaat ttcagattta ctcctcgcgt
     4321 ccaaaggcta aatgaactgg aggcccaaac tagagtgaaa ttgaactatt tggatcagat
     4381 tgcaaaattc tgggaaattc aaggctcctc tttaaagatt cccaatgtgg agcggaagat
     4441 cttggacctc tacagcctta gtaagcaatg taacacacac ccgtttgaca atgaggtaaa
     4501 agataaggaa tacaagcccc acagcatccc ccttagacag tctgtgcagc cttcaaagtt
     4561 cagcagctac agtcgacggg caaaaaggct acagcctgat ccagagccta cagaggagga
     4621 cattgagaag catccagagc taaagaagtt acagatatat gggccaggtc ccaaaatgat
     4681 gggcttgggc cttatggcta aggataagga taagactgtg cataagaaag tcacatgccc
     4741 cccaactgtt acggtgaagg atgagcaaag tggaggtggg aacgtgtcat caacattgct
     4801 caagcagcac ttgagcctag agccctgcac taagacaacc atgcaacttc gaaagaatca
     4861 cagcagtgcc cagtttattg actcatatat ttgccaagta tgctcccgtg gggatgaaga
     4921 tgataagctt cttttctgtg atggctgtga tgacaattac cacatcttct gcttgttacc
     4981 accccttcct gaaatcccca gaggcatctg gaggtgccca aaatgtatct tggcggagtg
     5041 taaacagcct cctgaagctt ttggatttga acaggctacc caggagtaca gtttgcagag
     5101 ttttggtgaa atggctgatt ccttcaagtc cgactacttc aacatgcctg tacatatggt
     5161 gcctacagaa cttgtagaga aggaattctg gaggctggtg agcagcattg aggaagacgt
     5221 gacagttgaa tatggagctg atattcattc caaagaattt ggcagtggct ttcctgtcag
     5281 caatagcaaa caaaacttat ctcctgagga gaaggagtat gcgaccagtg gttggaacct
     5341 gaatgtgatg ccagtgctag atcagtctgt tctctgtcac atcaatgcag acatctcagg
     5401 catgaaggtg ccctggctgt acgtgggcat ggttttctca gcattttgtt ggcatattga
     5461 ggatcactgg agttactcta ttaactatct gcattggggt gagccgaaga cctggtatgg
     5521 tgtaccctcc ctggcagcag agcatttgga ggaggtgatg aagatgctga cacctgagct
     5581 gtttgatagc cagcctgatc tcctacacca gcttgtcact ctcatgaatc ccaacacttt
     5641 gatgtcccat ggtgtgccag ttgtccgcac aaaccagtgt gcaggggagt ttgtcatcac
     5701 ttttcctcgt gcttaccaca gtggttttaa ccaaggctac aattttgctg aagctgtcaa
     5761 cttttgtact gctgactggc tacctgctgg acgccagtgc attgaacact accgccggct
     5821 ccggcgctat tgtgtcttct cccacgagga gctcatctgc aagatggctg ccttcccaga
     5881 gacgttggat ctcaatctag cagtagctgt gcacaaggag atgttcatta tggttcagga
     5941 ggagcgacgt ctacgaaagg cccttttgga gaagggcgtc acggaggctg agcgagaggc
     6001 ttttgagctg ctcccagatg atgaacgcca gtgcatcaag tgcaagacca cgtgcttctt
     6061 gtcagccctg gcctgctacg actgcccaga tggccttgta tgcctttccc acatcaatga
     6121 cctctgcaag tgctctagta gccgacagta cctccggtat cggtacacct tggatgagct
     6181 ccccaccatg ctgcataaac tgaagattcg ggctgagtct tttgacacct gggccaacaa
     6241 agtgcgagtg gccttggagg tggaggatgg ccgtaaacgc agctttgaag agctaagggc
     6301 actggagtct gaggctcgtg agaggaggtt tcctaatagt gagctgcttc agcgactgaa
     6361 gaactgcctg agtgaggtgg aggcttgtat tgctcaagtc ctggggctgg tcagtggtca
     6421 ggtggccagg atggacactc cacagctgac cttgactgaa ctccgggtcc ttcttgagca
     6481 gatgggcagc ctgccctgtg ccatgcatca gattggggat gtcaaggatg tcctggaaca
     6541 ggtggaggcc tatcaagctg aggctcgtga ggctctggcc acactgccct ctagtccagg
     6601 gctattgcgg tccctgttgg agagggggca gcagctgggt gtagaggtgc ctgaagccca
     6661 tcagcttcag cagcaggtgg agcaggcgca atggctagat gaagtgaagc aggccctggc
     6721 cccttctgct cacaggggct ctctggtcat catgcagggg cttttggtta tgggtgccaa
     6781 gatagcctcc agcccttctg tggacaaggc ccgggctgag ctgcaagaac tactgaccat
     6841 tgcagagcgc tgggaagaaa aggctcattt ctgcctggag gccaggcaga agcatccacc
     6901 agccacattg gaagccataa ttcgtgagac agaaaacatc cctgttcacc tgcctaacat
     6961 ccaggctctc aaagaagctc tgactaaggc acaagcttgg attgctgatg tggatgagat
     7021 ccaaaatggt gaccactacc cctgtctaga tgacttggag ggcctggtgg ctgtgggccg
     7081 ggacctgcct gtggggctgg aagagctgag acagctagag ctgcaggtat tgacagcaca
     7141 ttcctggaga gagaaggcct ccaagacctt tctcaagaag aattcttgct acacactgct
     7201 tgaggtgctt tgcccgtgtg cagacgctgg ctcagacagc accaagcgta gccggtggat
     7261 ggagaaggcg ctggggttgt accagtgtga cacagagctg ctggggctgt ctgcacagga
     7321 cctcagagac ccaggctctg tgattgtggc cttcaaggaa ggggaacaga aggagaagga
     7381 gggtatcctg cagctgcgtc gcaccaactc agccaagccc agtccactgg caccatccct
     7441 catggcctct tctccgactt ctatctgtgt gtgtgggcag gtgccagctg gggtgggagt
     7501 tctgcagtgt gacctgtgtc aggactggtt ccatgggcag tgtgtgtcag tgccccatct
     7561 cctcacctct ccaaagccca gtctcacttc atctccactg ctagcctggt gggaatggga
     7621 cacaaaattc ctgtgtccac tgtgtatgcg ctcacgacgg ccacgcctag agacaatcct
     7681 agccttgctg gttgccctgc agaggctgcc cgtgcggctg cctgagggtg aggcccttca
     7741 gtgtctcaca gagagggcca ttggctggca agaccgtgcc agaaaggctc tggcctctga
     7801 agatgtgact gctctgttgc gacagctggc tgagcttcgc caacagctac aggccaaacc
     7861 cagaccagag gaggcctcag tctacacttc agccactgcc tgtgacccta tcagagaagg
     7921 cagtggcaac aatatttcta aggtccaagg gctgctggag aatggagaca gtgtgaccag
     7981 tcctgagaac atggctccag gaaagggctc tgacctggag ctactgtcct cactgttgcc
     8041 gcagttgact ggccctgtgt tggagctgcc tgaggcaatc cgggctcccc tggaggagct
     8101 catgatggaa ggggacctgc ttgaggtgac cctggatgag aaccacagca tctggcagct
     8161 gctgcaggct ggacagcctc cagacctgga cagaattcgc acacttctgg agctggaaaa
     8221 atttgaacat caagggagtc ggacaaggag ccgggctctg gagaggcgac ggcggcggca
     8281 gaaggtggat cagggtagaa acgttgagaa tcttgttcaa caggagcttc agtcaaaaag
     8341 ggctcggagc tcagggatta tgtctcaggt gggccgagaa gaagaacatt atcaggagaa
     8401 agcagaccgt gaaaatatgt tcctgacacc ttccacagac cacagccctt tcttgaaagg
     8461 aaaccaaaat agcttacaac acaaggattc aggctcttca gctgcttgtc cttctttaat
     8521 gcctttgcta caactctcct actctgatga gcaacagttg tagtaatgaa ctagtccgta
     8581 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aatgtcgatt
     8641 gttcataaca gatcgacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     8701 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     8761 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     8821 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     8881 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     8941 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     9001 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     9061 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     9121 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     9181 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     9241 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     9301 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     9361 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     9421 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     9481 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     9541 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     9601 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     9661 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     9721 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     9781 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9841 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     9901 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     9961 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
    10021 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    10081 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
    10141 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
    10201 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
    10261 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
    10321 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
    10381 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
    10441 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
    10501 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
    10561 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
    10621 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    10681 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
    10741 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    10801 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    10861 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    10921 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
    10981 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    11041 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    11101 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    11161 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    11221 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    11281 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    11341 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    11401 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    11461 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    11521 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    11581 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    11641 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    11701 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    11761 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    11821 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    11881 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    11941 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    12001 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    12061 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    12121 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    12181 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    12241 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    12301 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    12361 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    12421 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    12481 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    12541 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    12601 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    12661 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    12721 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
    12781 ctcactaaag ggaacaaaag ctggagctgc aagc
//
