LOCUS       sequence-400488-       11527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11527)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2486
FEATURES             Location/Qualifiers
     source          1..11527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7384..7972
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7437..7457)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7855..7866)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8044..8277
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8355..8476
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8392..8411)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8446..8465
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8516..8651
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8671..8690)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8672..8690)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8700..8717)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8700..8716)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8709..8731)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8858..9313
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8945..8964)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9155..9176
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9339..9443
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9444..9512,9513..10304)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9662..9681)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10475..11063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10964..10983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11217..11234
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11351..11372
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11387..11392,11393..11410,11411..11417)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11425..11441
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11430..11452
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11449..11465
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11449..11465
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11484..11504
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11486..11504
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtca agcgaggaaa gctaccgggc
     4141 catcctgcgt tacctgacga acgagcgcga gccgtatgcg ccgggcaccg agggcaatgt
     4201 caagcgtaaa atccgaaaag ctgccgcctg ctacgtggtg cgcggcggga ctctgtatta
     4261 ccagcggcgg cagcggcacc gcaagacctt cgcggagctg gaggtggtgc tgcagccgga
     4321 gcgacgccgg gacctcatcg aggcggcgca cctgggtccc ggcggcactc accacacccg
     4381 gcatcagacc tggcactact tgtccaagac gtactggtgg cgaggtatat tgaagcaagt
     4441 caaagattac attaaacagt gtagcaaatg ccaggagaaa ctagatcgat cccgtccaat
     4501 atcagatgtt tcagaaatgt tggaagaatt gggactagac cttgaatctg gagaagaaag
     4561 taatgaatcg gaagatgacc tgagcaactt tacttcatct ccaactacag catccaagcc
     4621 tgcaaaaaag aagccagtat ccaaacatga acttgtgttt gttgacacca aaggagtggt
     4681 aaaacgttct tctccaaaac attgtcaggc tgtcttaaaa cagctgaacg aacagagact
     4741 ttccaaccag ttctgtgatg ttactttgtt aattgaagga gaagagtaca aagctcataa
     4801 atctgttttg tcagcaaata gcgagtattt tcgagatctt tttattgaga aaggagctgt
     4861 ttccagtcat gaggctgtgg tggatctttc tggtttttgt aaggccagct tccttccttt
     4921 actggaattt gcctatactt ctgtactaag ttttgatttc tgtagcatgg ctgatgtagc
     4981 catcttagct cgtcatcttt tcatgtcaga agtcttagag atttgtgaaa gtgtacataa
     5041 gctaatggaa gagaagcagc taacagtata taagaagggc gaagtacaaa cagttgcatc
     5101 cacccaggac ttacgagtac agaatggagg tacagcacct cctgttgcta gcagtgaggg
     5161 aaccacaaca agtttaccta ctgaacttgg ggattgtgaa attgtactac tggtaaatgg
     5221 agaattgcca gaagctgagc agaatggaga ggtaggacga cagcctgagc cccaggtttc
     5281 ttcagaggct gaatctgccc tgtcatcagt aggatgtata gctgattccc atcctgaaat
     5341 ggagtctgtt gatttaataa caaaaaacaa ccagacagaa ctagaaactt caaacaacag
     5401 agaaaataac acagtttcta atatacaccc taaactttca aaagagaatg taattagtag
     5461 ctcgccagag gatagtggta tgggaaatga tatatcagct gaggatattt gtgccgaaga
     5521 cattccaaaa cataggcaga aagttgacca acctttaaaa gatcaggaaa atctagttgc
     5581 atcaacagca aagacagact ttggccctga tgatgatact tatagaagca ggcttcgaca
     5641 acgttctgtt aatgaagggg catatattcg actacacaag ggaatggaga aaaagctgca
     5701 gaaacggaaa gccgttccca agtcagcagt tcaacaggtg gctcagaagt tagttcaaag
     5761 aggaaaaaag atgaaacagc caaaaagaga tgctaaagag aacacagaag aagcatctca
     5821 taaatgtggg gaatgtggaa tggtttttca gagacgatac gcccttataa tgcacaaact
     5881 gaaacatgaa agagctagag attacaaatg tccattgtgt aaaaaacagt ttcagtacag
     5941 tgcctctttg cgagcacatc ttattcgtca taccagaaaa gatgcaccct cttcatcctc
     6001 gtccaattcc acgtctaatg aagcatcggg aacatcatct gagaagggca gaaccaagcg
     6061 ggaatttata tgttccatat gtggaagaac attacctaaa ttatattctc tccgaataca
     6121 tatgttaaag cacacaggtg taaagccaca tgcatgccag gtctgtggaa agacttttat
     6181 ctataagcat ggtctaaaat tacatcagag tcttcatcaa tcacagaagc agttccagtg
     6241 tgaactgtgt gttaagtcat ttgttaccaa acggagtctt caagaacata tgagtattca
     6301 cacaggagag tccaagtacc tttgctcagt ttgtggaaag tcttttcata ggggctctgg
     6361 actcagcaag cacttcaaga aacaccaacc aaagcctgag gttcgaggct atcattgtac
     6421 tcaatgtgaa aaaagtttct ttgaagctag agatcttcgc cagcacatga acaaacatct
     6481 tggtgtgaag ccattccagt gccaattttg tgataagtgc tatagttgga agaaagattg
     6541 gtattcccat gtgaagtctc attctgtcac tgagccttat aggtgtaata tatgtggcaa
     6601 agaattttat gaaaaagctt tgttcagaag gcatgtaaag aaagctaccc atgggaagaa
     6661 aggaagagca aagcaaaacc tggaacgggt gtgtgaaaaa tgtggaagaa aattcactca
     6721 gctaagagag tataggagac acatgaacaa ccatgaagga gttaagccat ttgagtgctt
     6781 aacatgtgga gtagcttggg ctgatgcccg atctctaaaa cgccatgtca gaacacatac
     6841 tggtgaacgg ccctatgtct gtcctgtatg tagcgaagcc tacatagatg ctcgaacact
     6901 ccgtaaacat atgactaaat tccacagaga ctatgtgcct tgcaaaatta tgctggaaaa
     6961 agacaccctt cagtttcata accaaggaac tcaagtggca catgctgtta gcatcttaac
     7021 agcaggcatg caggaacaag aaagcagtgg tcctcaagaa cttgagactg tggtagtgac
     7081 aggagaaact atggaagctc tggaagctgt tgcagctact gaagagtatc catcggtatc
     7141 tacactttct gaccaaagta ttatgcaagt ggttaattat gtattagcac aacagcaagg
     7201 acagaagcta tctgaagttg cagaagctat tcaaactgtt aaagtagagg tagcacatat
     7261 ttcaggagga gaatagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct
     7321 ttatatatct tgtggaaagg acgaggacta tagatcgcga taactacaac gcgttaagtc
     7381 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7441 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7501 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7561 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7621 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7681 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7741 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     7801 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7861 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7921 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     7981 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     8041 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     8101 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     8161 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8221 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     8281 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     8341 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     8401 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     8461 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     8521 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8581 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     8641 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     8701 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     8761 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     8821 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     8881 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     8941 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     9001 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     9061 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     9121 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     9181 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     9241 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     9301 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     9361 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     9421 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     9481 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     9541 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     9601 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     9661 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     9721 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     9781 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     9841 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     9901 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     9961 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
    10021 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
    10081 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
    10141 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
    10201 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
    10261 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
    10321 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    10381 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    10441 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    10501 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    10561 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    10621 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    10681 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    10741 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    10801 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    10861 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    10921 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    10981 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    11041 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    11101 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    11161 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    11221 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    11281 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    11341 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    11401 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    11461 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
    11521 tgcaagc
//
