LOCUS       sequence-400496-       14608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14608)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 14608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2745
FEATURES             Location/Qualifiers
     source          1..14608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    10465..11053
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10518..10538)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10936..10947)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11125..11358
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11436..11557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11473..11492)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11527..11546
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11597..11732
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11752..11771)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11753..11771)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11781..11798)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11781..11797)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11790..11812)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11939..12394
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12026..12045)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12236..12257
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12420..12524
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12525..12593,12594..13385)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12743..12762)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13556..14144
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14045..14064
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14298..14315
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14432..14453
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14468..14473,14474..14491,14492..14498)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14506..14522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14511..14533
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14530..14546
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14530..14546
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14565..14585
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14567..14585
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgagc tcctccccct cccgcagtgc
     4141 cgccgacatc tacatccggg agtatttcca cagccacgtc agcggcggcc accccgaggc
     4201 cactcctctc cgtgtgatgt acacggaccg gccgctgagc cagacggacc cagtcacgct
     4261 gcagtactgt tgcctgaccg acgaccgcca ggctttccgc ccgcccacac gggcagagct
     4321 ggcgcggcac cgcgtggtgg tcaccaccac ctcccaggcc cgtgagctca gggtgccggt
     4381 cggcttcttc tcccacattc tcatcgatga ggcggcccag atgctggagt gcgaggccct
     4441 caccccgctg gcctatgcct cgcacggcac ccgcctcgtg ctggcgggcg accacatgca
     4501 ggtcacaccc cggctgttca gtgtggccag ggcccgggcg gccgagcaca cgctgctgca
     4561 ccgcctcttc ctgtgctacc agcaggagac tcacgaggtg gcgcggcaga gccgcctggt
     4621 cttccacgag aactaccgct gcacggacgc cattgtcagc ttcatctcgc ggcacttcta
     4681 cgtggccaag ggcaacccca tccacgccag gggcaaggtt ccgccccacc cccggcacta
     4741 cccgctcatg ttctgccacg tggcgggcag cccagaccgg gacatgtcca tggcgtcctg
     4801 gctgaatctg gctgagattg cgcaggtcgt cgagaaggtg caggaggcct acaacacctg
     4861 gcccagctgc tggggcggcc gcgagcagag gtgcatctgt gtcgtttccc acggtgccca
     4921 ggtcagtgca ctgaggcagg agctgaggag gcgggaccta ggccaggtgt ctgtcggcag
     4981 ttttgagatc ctgccagggc ggcagttccg ggtcgtggtg ctcagcactg tgcacacctg
     5041 ccagagcctg ctcagccctg gggcactggc ccctgagttc ttcaccgacg cccgcgtgct
     5101 caacaccgtc ctgacccgcg cccagtccca gctggtggta gtgggggacg ccgtggccct
     5161 ctgctccttc ggggcctgcg gcaagctctg ggagagcttc atccgtgagt gcgtggagcg
     5221 gcacagtgtc tgccccgagg gcctgtccat ggagcaggtc gagcagggtg tggcgcagag
     5281 acggcgctgg cctccccgag gcacacaggc tggggcagcg gggaactggg aggctgcccc
     5341 agagccagta ggggacctgg ccgaggagca ggcggctgtg gtgacggcca tggtgaaggc
     5401 agagccggga gatgaggctc tgagcccagc atcccgtgac atcacggcaa ccacagcgca
     5461 gacggaggct gcggcagcac cagcaggaga cgcagtgaag gaagacgtgg tgcccggggc
     5521 ctgtgcggca ggagcggctg ctgcagcggg cgtggagtcc acggaggctg aggatgcaga
     5581 ggctgacttc tggccgtggg acggggagct caacgctgac gacgccatcc tacgggagct
     5641 tctggacgag agccagaagg tgatggtgac cgtcggggag gacgggctgc tggacactgt
     5701 cgccaggccc gagtccctgc agcaggcccg gctgtacgag aacctgcccc cggctgcgct
     5761 acggaagctg ctgcacgcgg agcccgagcg gtaccgccac tgctctttcg tgccagagac
     5821 cttcgagcgg gcgtcagcca tcccgctgga cgatgcctcc tcgggcccca tccaggtcag
     5881 gggccgcctg gactgtggga tggccttcgc cggggatgag gtgctggtgc agctcctttc
     5941 gggagacaag gcgcccgagg ggcggcttcg gggccgcgtg ctgggcgtgc tgaagaggaa
     6001 gaggcacgag ctggcgtttg tgtgccgcat ggacacgtgg gacccgcgca tcatggtccc
     6061 catcaatggc tccgtgacca agatcttcgt ggccgagctg aaggacccat cgcaggtccc
     6121 catctacagc ctccggaagg gccggctgca gcgtgtgggg cttgagaggc tcaccgccga
     6181 ggcccggcac agccggctct tctgggtcca aattgtcctg tggcggcaag gcttctacta
     6241 cccgctgggc atcgtccggg aggtgctgcc tgaggccagc acctgggagc agggcctccg
     6301 catcctcggc ctggagtaca gcttgagggt gcccccgtcg gaccaggcca ccatcaccaa
     6361 ggtgctgcag aaataccaca cggagcttgg ccgggttgcc ggccgccgag aggactgccg
     6421 cgccttcttg accttcactg tggaccccca gggcgcctgc aacctcgatg atgccctcag
     6481 tgtccgagac ctgggtccca ggtgcgaggt ggctgtgcac atcactgatg tggccagctt
     6541 cgtgcccagg gacggggtgc tggacgtgga ggcgcgaagg cagggcgctg cgttctatgc
     6601 ccccggcagg gagccagtgc ccatgctgcc ggccagcctc tgccaggacg tcctcagcct
     6661 cctgcctggc cgggaccgcc tggccatctc cctgttcctc accatggaga aggccagtgg
     6721 ccagctgaag agcctgcgct ttgcaccctc cgtggtccag tctgaccgcc agctgtccta
     6781 cgaggaggcg gaggaggtga tcaggcagca cccgggtgcc ggccgtgagc tgccggcccg
     6841 cctggactcc gtggacgcct gcgtcgtggc cgcgtgctac ttctctcggc tgctgcgccg
     6901 gcaccgcctg cggtccgact gcttctatga gcagccggac gaggacggca ccctgggctt
     6961 ccgcgcggcc cacatcatgg tgaaggagta catgattcag tttaataggc tcgtggctga
     7021 gttcctggtg ggcagcgagt gcacgcggac ggtcacgcct ctgcggtggc agccagcacc
     7081 ccgcagccag cagctcaagg ccctgtgtga gaagcatggg gaccgggtgc ccctgtcact
     7141 gcacctcggc caccacctgc acggcggcgg gggcagtccc cccgacacgc ggctgcacct
     7201 cctggcctcc ctctggaagc aggtccagtt tgctgcccgc acccaggact acgaacagat
     7261 ggtggacttg gtcaccacgg acgacatgca cccattcctg gctcctgcag gccgcgacct
     7321 ccgcaaggcc ctggagcgct cggcgttcgg ccgctgcgcc cggggccacc agcagcaggg
     7381 cggccactac tcgctgcagg tggactggta cacgtgggcc acctcgccca tccgcaggta
     7441 cctggacgtg gtgttgcagc ggcagatcct gctggcgctg ggccatgggg gctctgccta
     7501 ctctgccagg gacatcgatg ggctctgcca ggccttcagc ctccagcacg cacttgccca
     7561 gagctatcag cggcgggcgc gcagcctgca cctggccgtg cagctcaagg cccagcctct
     7621 ggacaagctg ggcttcgtgg tggacgtgga ggcgggctcc cgctgcttcc ggctgctctt
     7681 ccccagcaac cgggagacgc tgcctgaccc ctgccccgtc ccctacggct ccctgcagct
     7741 ggccgagcac ccccacgccc tggcaggccg gccgggcctg cggctcctgt ggcggcgccg
     7801 tgtctactca gcgcagggat ccagcccgcc cctgccactg cctggcactg tgccggaccc
     7861 acacaccctg gccgtggaga cggccctgtg gaagcagctg ctggagctgg tggagctgca
     7921 gcgctggccg gaggcggctg ctctcatcca ggagaagggc gaggcgtccc agcggcggga
     7981 gctggtgcag gtgcagcgga gccactgtgg ccatttcctg gaggtggccc gggagctggg
     8041 cagtggggac accctgcagg tgcagctcgg caccagcctg cagcacggct tcctggtacc
     8101 gagccctcag ctctggacgg tggcaccggg cttcagcctc tgcctggagc acgtggagcg
     8161 gcccggagac tgcttctcag gccgtgtgta ccgggccccg agggaccggt accgcgacgt
     8221 ggatgagtac gcctgcgtgt gggaaccatt ctgcgccctg gagtcggcca ccggcgcggt
     8281 tgccgagaat gactccgtca cacttcagca cctgagtgtc tcctgggagg cgtcacggac
     8341 gccgcagggg cagctgcagg gcgccttccg cctggaggcc gccttcctcg aggagaactg
     8401 tgccgacatc aacttcagct gctgctacct ctgcatccgg ctcgaggggc tgccggctcc
     8461 cacggccagc ccacgccctg ggcccagcag cctcggccct ggcctgaatg ttgaccccgg
     8521 cacgtatacc tgggtggccc acgggcagac gcaggactgg gaccaggagc gccgggcaga
     8581 ccggcaggag gctcccagac gggtgcacct cttcgtccac cacatgggca tggagaaggt
     8641 tccggaagag gtgctgaggc cgggcaccct gttcaccgtt gagctgctgc ccaagcagct
     8701 tcctgatctc cgcaaggagg aagccgtgcg tggactagag gaggcgtccc cgctggtcac
     8761 cagcatcgca ctgggccggc ctgtcccgca gcccctctgc agagtgatcc ccagcaggtt
     8821 cctggagcgg cagacctaca acatccccgg aggccgccac aagctgaacc ccagccagaa
     8881 cgtggcggtc agggaggctc tggagaagcc tttcacggtc attcagggcc caccaggtac
     8941 agggaagacg atcgtgggcc tccacatcgt attctggttt cataaatcaa accaggagca
     9001 ggtgcagccc ggaggccccc cccgtgggga gaagcggctg gggggtccct gcatcttgta
     9061 ctgcggcccc tccaacaagt cggtggatgt cctggcagga ctgctcctga gaaggatgga
     9121 gctgaagccc ctccgtgtgt acagtgagca ggctgaggcc agcgagttcc cagtgccgcg
     9181 tgtgggcagc aggaagctgc tcaggaagag cccccgggag gggaggccga accagagcct
     9241 caggagcatc accctgcacc accggatccg gcaggccccc aacccttact cgtcggaaat
     9301 caaggccttt gacacccggc tgcagagagg ggagctcttc tccagggagg acctggtctg
     9361 gtacaagaag gtcttgtggg aggctcggaa gttcgagctg gaccggcatg aggtcatcct
     9421 ctgcacctgc tcctgtgcag cctctgccag cctcaaaatc ctggacgtga ggcagatcct
     9481 tgttgacgag gcaggcatgg ccacggaacc tgaaaccctc atccccctgg tgcagttccc
     9541 acaggccgag aaggtggttc ttctcggaga ccacaagcag ctgcggcctg tggtcaagaa
     9601 tgagcggctg caaaacctgg gtctggaccg gtctctgttc gagcggtacc acgaggacgc
     9661 acatatgctg gacactcagt accgcatgca tgagggcatc tgtgccttcc cctctgtggc
     9721 gttctacaag agcaagctga agacgtggca gggcctgagg aggccgccca gtgtcctggg
     9781 ccacgctggc aaggagagct gccctgtcat ctttggccac gtgcagggcc acgagcggag
     9841 cctgctggtg tccacggacg aagggaatga gaactccaag gccaacctgg aggaggtggc
     9901 tgaggtggtc cgtatcacca agcagctgac cctggggagg accgtagagc cccaggacat
     9961 cgccgtcctc acgccctaca acgcgcaggc ctctgagatc agcaaggccc ttcggcgaga
    10021 gggcatcgcc ggggtggccg tgtcctccat caccaagagc caggggagcg agtggcgcta
    10081 tgtgctggtg agcaccgtcc gcacctgtgc caagagcgac ctggaccagc ggcccaccaa
    10141 gagctggctc aagaagtttc tgggcttcgt tgtggacccc aaccaagtga atgtggctgt
    10201 cacgcgggcc caggaggggc tctgcctgat cggagaccac ctccttctgc gctgctgccc
    10261 cctctggcgt agcctcctgg acttctgcga ggctcagcag accctcgtgc ctgccggcca
    10321 ggtgcgcgtc tgcaggaggc caactatgcc ttcctagtaa tgaactagtc cgtaacttga
    10381 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgatcagg taatcgtaga
    10441 catataacta cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
    10501 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
    10561 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
    10621 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
    10681 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
    10741 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
    10801 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
    10861 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
    10921 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
    10981 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
    11041 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
    11101 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
    11161 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
    11221 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
    11281 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
    11341 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
    11401 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
    11461 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    11521 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
    11581 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
    11641 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
    11701 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
    11761 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
    11821 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
    11881 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
    11941 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
    12001 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
    12061 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
    12121 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
    12181 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
    12241 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
    12301 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
    12361 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
    12421 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    12481 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    12541 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    12601 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    12661 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    12721 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    12781 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    12841 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    12901 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    12961 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    13021 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    13081 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    13141 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    13201 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    13261 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    13321 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    13381 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    13441 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    13501 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    13561 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    13621 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    13681 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    13741 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    13801 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    13861 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    13921 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    13981 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    14041 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    14101 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    14161 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    14221 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    14281 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    14341 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    14401 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    14461 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    14521 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
    14581 aaagggaaca aaagctggag ctgcaagc
//
