LOCUS       sequence-400515-       15235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15235)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 15235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2428
FEATURES             Location/Qualifiers
     source          1..15235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4364..4381
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4385..4402
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(5363..5374)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    11092..11680
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11145..11165)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11563..11574)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11752..11985
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12063..12184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12100..12119)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12154..12173
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12224..12359
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12379..12398)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12380..12398)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12408..12425)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12408..12424)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12417..12439)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12566..13021
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12653..12672)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12863..12884
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13047..13151
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13152..13220,13221..14012)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13370..13389)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14183..14771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14672..14691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14925..14942
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    15059..15080
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15095..15100,15101..15118,15119..15125)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15133..15149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15138..15160
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15157..15173
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15157..15173
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     15192..15212
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        15194..15212
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcc cataacgcgg gcgccgcggc
     4141 cgccgccggc acccacagcg ccaagagcgg cggctccgag gcggctctca aggagggtgg
     4201 aagcgccgcc gcgctgtcct cctcctcctc ctcctccgcg gcggcagcgg cggcatcctc
     4261 ttcctcctcg tcgggcccgg gctcggccat ggagacgggg ctgctcccca accacaaact
     4321 gaaaaccgtt ggcgaagccc ccgccgcgcc gccccaccag cagcaccacc accaccacca
     4381 tgcccaccac caccaccacc atgcccacca cctccaccac caccacgcac tacagcagca
     4441 gctaaaccag ttccagcagc agcagcagca gcagcaacag cagcagcagc agcagcagca
     4501 acagcaacat cccatttcca acaacaacag cttgggcggc gcgggcggcg gcgcgcctca
     4561 gcccggcccc gacatggagc agccgcaaca tggaggcgcc aaggacagtg ctgcgggcgg
     4621 ccaggccgac cccccgggcc cgccgctgct gagcaagccg ggcgacgagg acgacgcgcc
     4681 gcccaagatg ggggagccgg cgggcggccg ctacgagcac ccgggcttgg gcgccctggg
     4741 cacgcagcag ccgccggtcg ccgtgcccgg gggcggcggc ggcccggcgg ccgtcccgga
     4801 gtttaataat tactatggca gcgctgcccc tgcgagcggc ggccccggcg gccgcgctgg
     4861 gccttgcttt gatcaacatg gcggacaaca aagccccggg atggggatga tgcactccgc
     4921 ctccgccgcc gccgccgggg cccccggcag catggacccc ctgcagaact cccacgaagg
     4981 gtaccccaac agccagtgca accattatcc gggctacagc cggcccggcg cgggcggcgg
     5041 cggcggcggc ggcggcggag gaggaggagg cagcggagga ggaggaggag gaggaggagc
     5101 aggagcagga ggagcaggag cgggagctgt ggcggcggcg gccgcggcgg cggcggcagc
     5161 agcaggaggc ggcggcggcg gcggctatgg gggctcgtcc gcggggtacg gggtgctgag
     5221 ctccccccgg cagcagggcg gcggcatgat gatgggcccc gggggcggcg gggccgcgag
     5281 cctcagcaag gcggccgccg gctcggcggc ggggggcttc cagcgcttcg ccggccagaa
     5341 ccagcacccg tcgggggcca ccccgaccct caatcagctg ctcacctcgc ccagccccat
     5401 gatgcggagc tacggcggca gctaccccga gtacagcagc cccagcgcgc cgccgccgcc
     5461 gccgtcgcag ccccagtccc aggcggcggc ggcgggggcg gcggcgggcg gccagcaggc
     5521 ggccgcgggc atgggcttgg gcaaggacat gggcgcccag tacgccgctg ccagcccggc
     5581 ctgggcggcc gcgcaacaaa ggagtcaccc ggcgatgagc cccggcaccc ccggaccgac
     5641 catgggcaga tcccagggca gcccaatgga tccaatggtg atgaagagac ctcagttgta
     5701 tggcatgggc agtaaccctc attctcagcc tcagcagagc agtccgtacc caggaggttc
     5761 ctatggccct ccaggcccac agcggtatcc aattggcatc cagggtcgga ctcccggggc
     5821 catggccgga atgcagtacc ctcagcagca gatgccacct cagtatggac agcaaggtgt
     5881 gagtggttac tgccagcagg gccaacagcc atattacagc cagcagccgc agcccccgca
     5941 cctcccaccc caggcgcagt atctgccgtc ccagtcccag cagaggtacc agccgcagca
     6001 ggacatgtct caggaaggct atggaactag atctcaacct cctctggccc ccggaaaacc
     6061 taaccatgaa gacttgaact taatacagca agaaagacca tcaagtttac cagatctgtc
     6121 tggctccatt gatgacctcc ccacgggaac ggaagcaact ttgagctcag cagtcagtgc
     6181 atccgggtcc acgagcagcc aaggggatca gagcaacccg gcgcagtcgc ctttctcccc
     6241 acatgcgtcc cctcatctct ccagcatccc ggggggccca tctccctctc ctgttggctc
     6301 tcctgtagga agcaaccagt ctcgatctgg cccaatctct cctgcaagta tcccaggtag
     6361 tcagatgcct ccgcagccac ccgggagcca gtcagaatcc agttcccatc ccgccttgag
     6421 ccagtcacca atgccacagg aaagaggttt tatggcaggc acacaaagaa accctcagat
     6481 ggctcagtat ggacctcaac agacaggacc atccatgtcg cctcatcctt ctcctggggg
     6541 ccagatgcat gctggaatca gtagctttca gcagagtaac tcaagtggga cttacggtcc
     6601 acagatgagc cagtatggac cacaaggtaa ctactccaga cccccagcgt atagtggggt
     6661 gcccagtgca agctacagcg gcccagggcc cggtatgggt atcagtgcca acaaccagat
     6721 gcatggacaa gggccaagcc agccatgtgg tgctgtgccc ctgggacgaa tgccatcagc
     6781 tgggatgcag aacagaccat ttcctggaaa tatgagcagc atgaccccca gttctcctgg
     6841 catgtctcag cagggagggc caggaatggg gccgccaatg ccaactgtga accgtaaggc
     6901 acaggaggca gccgcagcag tgatgcaggc tgctgcgaac tcagcacaaa gcaggcaagg
     6961 cagtttcccc ggcatgaacc agagtggact tatggcttcc agctctccct acagccagcc
     7021 catgaacaac agctctagcc tgatgaacac gcaggcgccg ccctacagca tggcgcccgc
     7081 catggtgaac agctcggcag catctgtggg tcttgcagat atgatgtctc ctggtgaatc
     7141 caaactgccc ctgcctctca aagcagacgg caaagaagaa ggcactccac agcccgagag
     7201 caagtcaaag gatagctaca gctctcaggg tatttctcag cccccaaccc caggcaacct
     7261 gccagtccct tccccaatgt cccccagctc tgctagcatc tcctcatttc atggagatga
     7321 aagtgatagc attagcagcc caggctggcc aaagactcca tcaagcccta agtccagctc
     7381 ctccaccact actggggaga agatcacgaa ggtgtacgag ctggggaatg agccagagag
     7441 aaagctctgg gtcgaccgat acctcacctt catggaagag agaggctctc ctgtctcaag
     7501 tctgcctgcc gtgggcaaga agcccctgga cctgttccga ctctacgtct gcgtcaaaga
     7561 gatcgggggt ttggcccagg ttaataaaaa caagaagtgg cgtgagctgg caaccaacct
     7621 aaacgttggc acctcaagca gtgcagcgag ctccctgaaa aagcagtata ttcagtacct
     7681 gtttgccttt gagtgcaaga tcgaacgtgg ggaggagccc ccgccggaag tcttcagcac
     7741 cggggacacc aaaaagcagc ccaagctcca gccgccatct cctgctaact cgggatcctt
     7801 gcaaggccca cagacccccc agtcaactgg cagcaattcc atggcagagg ttccaggtga
     7861 cctgaagcca cctaccccag cctccacccc tcacggccag atgactccaa tgcaaggtgg
     7921 aagaagcagt acaatcagtg tgcacgaccc attctcagat gtgagtgatt catccttccc
     7981 gaaacggaac tccatgactc caaacgcccc ctaccagcag ggcatgagca tgcccgatgt
     8041 gatgggcagg atgccctatg agcccaacaa ggaccccttt gggggaatga gaaaagtgcc
     8101 tggaagcagc gagcccttta tgacgcaagg acagatgccc aacagcagca tgcaggacat
     8161 gtacaaccaa agtccctccg gagcaatgtc taacctgggc atggggcagc gccagcagtt
     8221 tccctatgga gccagttacg accgaaggca tgaaccttat gggcagcagt atccaggcca
     8281 aggccctccc tcgggacagc cgccgtatgg agggcaccag cccggcctgt acccacagca
     8341 gccgaattac aaacgccata tggacggcat gtacgggccc ccagccaagc gccacgaggg
     8401 cgacatgtac aacatgcagt acagcagcca gcagcaggag atgtacaacc agtatggagg
     8461 ctcctactcg ggcccggacc gcaggcccat ccagggccag tacccgtatc cctacagcag
     8521 ggagaggatg cagggcccgg ggcagatcca gacacacgga atcccgcctc agatgatggg
     8581 cggcccgctg cagtcgtcct ccagtgaggg gcctcagcag aatatgtggg cagcacgcaa
     8641 tgatatgcct tatccctacc agaacaggca gggccctggc ggccctacac aggcgccccc
     8701 ttacccaggc atgaaccgca cagacgatat gatggtaccc gatcagagga taaatcatga
     8761 gagccagtgg ccttctcacg tcagccagcg tcagccttat atgtcgtcct cagcctccat
     8821 gcagcccatc acacgcccac cacagccgtc ctaccagacg ccaccgtcac tgccaaatca
     8881 catctccagg gcgcccagcc cagcgtcctt ccagcgctcc ctggagaacc gcatgtctcc
     8941 aagcaagtct ccttttctgc cgtctatgaa gatgcagaag gtcatgccca cggtccccac
     9001 atcccaggtc accgggccac caccccaacc acccccaatc agaagggaga tcacctttcc
     9061 tcctggctca gtagaagcat cacaaccagt cttgaaacaa aggcgaaaga ttacctccaa
     9121 agatatcgtt actcctgagg cgtggcgtgt gatgatgtcc cttaaatcag gtcttttggc
     9181 tgagagtacg tgggctttgg acactattaa tattcttctg tatgatgaca gcactgttgc
     9241 tactttcaat ctctcccagt tgtctggatt tctcgaactt ttagtcgagt actttagaaa
     9301 atgcctgatt gacatttttg gaattcttat ggaatatgaa gtgggagacc ccagccaaaa
     9361 agcacttgat cacaacgcag caaggaagga tgacagccag tccttggcag acgattctgg
     9421 gaaagaggag gaagatgctg aatgtattga tgacgacgag gaagacgagg aggatgagga
     9481 ggaagacagc gagaagacag aaagcgatga aaagagcagc atcgctctga ctgccccgga
     9541 cgccgctgca gacccaaagg agaagcccaa gcaagccagt aagttcgaca agctgccaat
     9601 aaagatagtc aaaaagaaca acctgtttgt tgttgaccga tctgacaagt tggggcgtgt
     9661 gcaggagttc aatagtggcc ttctgcactg gcagctcggc gggggtgaca ccaccgagca
     9721 cattcagact cactttgaga gcaagatgga aattcctcct cgcaggcgcc cacctccccc
     9781 cttaagctcc gcaggtagaa agaaagagca agaaggcaaa ggcgactctg aagagcagca
     9841 agagaaaagc atcatagcaa ccatcgatga cgtcctctct gctcggccag gggcattgcc
     9901 tgaagacgca aaccctgggc cccagaccga aagcagtaag tttccctttg gtatccagca
     9961 agccaaaagt caccggaaca tcaagctgct ggaggacgag cccaggagcc gagacgagac
    10021 tcctctgtgt accatcgcgc actggcagga ctcgctggct aagcgatgca tctgtgtgtc
    10081 caatattgtc cgtagcttgt cattcgtgcc tggcaatgat gccgaaatgt ccaaacatcc
    10141 aggcctggtg ctgatcctgg ggaagctgat tcttcttcac cacgagcatc cagagagaaa
    10201 gcgagcaccg cagacctatg agaaagagga ggatgaggac aagggggtgg cctgcagcaa
    10261 agatgagtgg tggtgggact gcctcgaggt cttgagggat aacacgttgg tcacgttggc
    10321 caacatttcc gggcagctag acttgtctgc ttacacggaa agcatctgct tgccaatttt
    10381 ggatggcttg ctgcactgga tggtgtgccc gtctgcagag gcacaagatc cctttccaac
    10441 tgtgggaccc aactcggtcc tgtcgcctca gagacttgtg ctggagaccc tctgtaaact
    10501 cagtatccag gacaataatg tggacctgat cttggccact cctccattta gtcgtcagga
    10561 gaaattctat gctacattag ttaggtacgt tggggatcgc aaaaacccag tctgtcgaga
    10621 aatgtccatg gcgcttttat cgaaccttgc ccaaggggac gcactagcag caagggccat
    10681 agctgtgcag aaaggaagca ttggaaactt gataagcttc ctagaggatg gggtcacgat
    10741 ggcccagtac cagcagagcc agcacaacct catgcacatg cagcccccgc ccctggaacc
    10801 acctagcgta gacatgatgt gcagggcggc caaggctttg ctagccatgg ccagagtgga
    10861 cgaaaaccgc tcggaattcc ttttgcacga gggccggttg ctggatatct cgatatcagc
    10921 tgtcctgaac tctctggttg catctgtcat ctgtgatgta ctgtttcaga ttgggcagtt
    10981 atagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
    11041 tggaaaggac gagccatgtg gttgaagcag tctattacgc gttaagtcga caatcaacct
    11101 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
    11161 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
    11221 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
    11281 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
    11341 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
    11401 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
    11461 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
    11521 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
    11581 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
    11641 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
    11701 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
    11761 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
    11821 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    11881 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    11941 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
    12001 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
    12061 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    12121 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    12181 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
    12241 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    12301 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    12361 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
    12421 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
    12481 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
    12541 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
    12601 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    12661 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    12721 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    12781 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    12841 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    12901 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    12961 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
    13021 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    13081 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    13141 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    13201 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
    13261 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    13321 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    13381 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    13441 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    13501 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    13561 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    13621 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    13681 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    13741 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    13801 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    13861 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    13921 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    13981 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    14041 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    14101 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    14161 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    14221 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    14281 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    14341 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    14401 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    14461 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    14521 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    14581 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    14641 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    14701 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    14761 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    14821 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    14881 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    14941 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    15001 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    15061 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    15121 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    15181 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagc
//
