LOCUS       sequence-400547-       15223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15223)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 15223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2432
FEATURES             Location/Qualifiers
     source          1..15223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             6895..6903
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    11080..11668
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11133..11153)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11551..11562)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11740..11973
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12051..12172
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12088..12107)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12142..12161
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12212..12347
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12367..12386)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12368..12386)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12396..12413)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12396..12412)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12405..12427)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12554..13009
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12641..12660)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12851..12872
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13035..13139
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13140..13208,13209..14000)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13358..13377)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14171..14759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14660..14679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14913..14930
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    15047..15068
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15083..15088,15089..15106,15107..15113)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15121..15137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15126..15148
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15145..15161
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15145..15161
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     15180..15200
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        15182..15200
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcc gcgcaggtcg cccccgccgc
     4141 cgccagcagc ctgggcaacc cgccgccgcc gccgccctcg gagctgaaga aagccgagca
     4201 gcagcagcgg gaggaggcgg ggggcgaggc ggcggcggcg gcagcggccg agcgcgggga
     4261 aatgaaggca gccgccgggc aggaaagcga gggccccgcc gtggggccgc cgcagccgct
     4321 gggaaaggag ctgcaggacg gggccgagag caatgggggt ggcggcggcg gcggagccgg
     4381 cagcggcggc gggcccggcg cggagccgga cctgaagaac tcgaacggga acgcgggccc
     4441 taggcccgcc ctgaacaata acctcacgga gccgcccggc ggcggcggtg gcggcagcag
     4501 cgatggggtg ggggcgcctc ctcactcagc cgcggccgcc ttgccgcccc cagcctacgg
     4561 cttcgggcaa ccctacggcc ggagcccgtc tgccgtcgcc gccgccgcgg ccgccgtctt
     4621 ccaccaacaa catggcggac aacaaagccc tggcctggca gcgctgcaga gcggcggcgg
     4681 cgggggcctg gagccctacg cggggcccca gcagaactct cacgaccacg gcttccccaa
     4741 ccaccagtac aactcctact accccaaccg cagcgcctac cccccgcccg ccccggccta
     4801 cgcgctgagc tccccgagag gtggcactcc gggctccggc gcggcggcgg ctgccggctc
     4861 caagccgcct ccctcctcca gcgcctccgc ctcctcgtcg tcttcgtcct tcgctcagca
     4921 gcgcttcggg gccatggggg gaggcggccc ctccgcggcc ggcgggggaa ctccccagcc
     4981 caccgccacc cccaccctca accaactgct cacgtcgccc agctcggccc ggggctacca
     5041 gggctacccc gggggcgact acagtggcgg gccccaggac gggggcgccg gcaagggccc
     5101 ggcggacatg gcctcgcagt gttggggggc tgcggcggcg gcagctgcgg cggcggccgc
     5161 ctcgggaggg gcccaacaaa ggagccacca cgcgcccatg agccccggga gcagcggcgg
     5221 cggggggcag ccgctcgccc ggacccctca gccatccagt ccaatggatc agatgggcaa
     5281 gatgagacct cagccatatg gcgggactaa cccatactcg cagcaacagg gacctccgtc
     5341 aggaccgcag caaggacatg ggtacccagg gcagccatac gggtcccaga ccccgcagcg
     5401 gtacccgatg accatgcagg gccgggcgca gagtgccatg ggcggcctct cttatacaca
     5461 gcagattcct ccttatggac aacaaggccc cagcgggtat ggtcaacagg gccagactcc
     5521 atattacaac cagcaaagtc ctcaccctca gcagcagcag ccaccctact cccagcaacc
     5581 accgtcccag acccctcatg cccaaccttc gtatcagcag cagccacagt ctcaaccacc
     5641 acagctccag tcctctcagc ctccatactc ccagcagcca tcccagcctc cacatcagca
     5701 gtccccggct ccatacccct cccagcagtc gacgacacag cagcaccccc agagccagcc
     5761 cccctactca cagccacagg ctcagtctcc ttaccagcag cagcaacctc agcagccagc
     5821 accctcgacg ctctcccagc aggctgcgta tcctcagccc cagtctcagc agtcccagca
     5881 aactgcctat tcccagcagc gcttccctcc accgcaggag ctatctcaag attcatttgg
     5941 gtctcaggca tcctcagccc cctcaatgac ctccagtaag ggagggcaag aagatatgaa
     6001 cctgagcctt cagtcaagac cctccagctt gcctgatcta tctggttcaa tagatgacct
     6061 ccccatgggg acagaaggag ctctgagtcc tggagtgagc acatcaggga tttccagcag
     6121 ccaaggagag cagagtaatc cagctcagtc tcctttctct cctcatacct cccctcacct
     6181 gcctggcatc cgaggccctt ccccgtcccc tgttggctct cccgccagtg ttgctcagtc
     6241 tcgctcagga ccactctcgc ctgctgcagt gccaggcaac cagatgccac ctcggccacc
     6301 cagtggccag tcggacagca tcatgcatcc ttccatgaac caatcaagca ttgcccaaga
     6361 tcgaggttat atgcagagga acccccagat gccccagtac agttcccccc agcccggctc
     6421 agccttatct ccgcgtcagc cttccggagg acagatacac acaggcatgg gctcctacca
     6481 gcagaactcc atggggagct atggtcccca ggggggtcag tatggcccac aaggtggcta
     6541 ccccaggcag ccaaactata atgccttgcc caatgccaac taccccagtg caggcatggc
     6601 tggaggcata aaccccatgg gtgccggagg tcaaatgcat ggacagcctg gcatcccacc
     6661 ttatggcaca ctccctccag ggaggatgag tcacgcctcc atgggcaacc ggccttatgg
     6721 ccctaacatg gccaatatgc cacctcaggt tgggtcaggg atgtgtcccc caccaggggg
     6781 catgaaccgg aaaacccaag aaactgctgt cgccatgcat gttgctgcca actctatcca
     6841 aaacaggccg ccaggctacc ccaatatgaa tcaagggggc atgatgggaa ctggacctcc
     6901 ttatggacaa gggattaata gtatggctgg catgatcaac cctcagggac ccccatattc
     6961 catgggtgga accatggcca acaattctgc agggatggca gccagcccag agatgatggg
     7021 ccttggggat gtaaagttaa ctccagccac caaaatgaac aacaaggcag atgggacacc
     7081 caagacagaa tccaaatcca agaaatccag ttcttctact acaaccaatg agaagatcac
     7141 caagttgtat gagctgggtg gtgagcctga gaggaagatg tgggtggacc gttatctggc
     7201 cttcactgag gagaaggcca tgggcatgac aaatctgcct gctgtgggta ggaaacctct
     7261 ggacctctat cgcctctatg tgtctgtgaa ggagattggt ggattgactc aggtcaacaa
     7321 gaacaaaaaa tggcgggaac ttgcaaccaa cctcaatgtg ggcacatcaa gcagtgctgc
     7381 cagctccttg aaaaagcagt atatccagtg tctctatgcc tttgaatgca agattgaacg
     7441 gggagaagac cctcccccag acatctttgc agctgctgat tccaagaagt cccagcccaa
     7501 gatccagcct ccctctcctg cgggatcagg atctatgcag gggccccaga ctccccagtc
     7561 aaccagcagt tccatggcag aaggaggaga cttaaagcca ccaactccag catccacacc
     7621 acacagtcag atccccccat tgccaggcat gagcaggagc aattcagttg ggatccagga
     7681 tgcctttaat gatggaagtg actccacatt ccagaagcgg aattccatga ctccaaaccc
     7741 tgggtatcag cccagtatga atacctctga catgatgggg cgcatgtcct atgagccaaa
     7801 taaggatcct tatggcagca tgaggaaagc tccagggagt gatcccttca tgtcctcagg
     7861 gcagggcccc aacggcggga tgggtgaccc ctacagtcgt gctgccggcc ctgggctagg
     7921 aaatgtggcg atgggaccac gacagcacta tccctatgga ggtccttatg acagagtgag
     7981 gacggagcct ggaatagggc ctgagggaaa catgagcact ggggccccac agccgaatct
     8041 catgccttcc aacccagact cggggatgta ttctcctagc cgctaccccc cgcagcagca
     8101 gcagcagcag cagcaacgac atgattccta tggcaatcag ttctccaccc aaggcacccc
     8161 ttctggcagc cccttcccca gccagcagac tacaatgtat caacagcaac agcagaatta
     8221 caagcggcca atggatggca catatggccc tcctgccaag cggcacgaag gggagatgta
     8281 cagcgtgcca tacagcactg ggcaggggca gcctcagcag cagcagttgc ccccagccca
     8341 gccccagcct gccagccagc aacaagctgc ccagccttcc cctcagcaag atgtatacaa
     8401 ccagtatggc aatgcctatc ctgccactgc cacagctgct actgagcgcc gaccagcagg
     8461 cggcccccag aaccaatttc cattccagtt tggccgagac cgtgtctctg caccccctgg
     8521 caccaatgcc cagcaaaaca tgccaccaca aatgatgggc ggccccatac aggcatcagc
     8581 tgaggttgct cagcaaggca ccatgtggca ggggcgtaat gacatgacct ataattatgc
     8641 caacaggcag agcacgggct ctgcccccca gggccccgcc tatcatggcg tgaaccgaac
     8701 agatgaaatg ctgcacacag atcagagggc caaccacgaa ggctcgtggc cttcccatgg
     8761 cacacgccag cccccatatg gtccctctgc ccctgtgccc cccatgacaa ggccccctcc
     8821 atctaactac cagcccccac caagcatgca gaatcacatt cctcaggtat ccagccctgc
     8881 tcccctgccc cggccaatgg agaaccgcac ctctcctagc aagtctccat tcctgcactc
     8941 tgggatgaaa atgcagaagg caggtccccc agtacctgcc tcgcacatag cacctgcccc
     9001 tgtgcagccc cccatgattc ggcgggatat caccttccca cctggctctg ttgaagccac
     9061 acagcctgtg ttgaagcaga ggaggcggct cacaatgaaa gacattggaa ccccggaggc
     9121 atggcgggta atgatgtccc tcaagtctgg tctcctggca gagagcacat gggcattaga
     9181 taccatcaac atcctgctgt atgatgacaa cagcatcatg accttcaacc tcagtcagct
     9241 cccagggttg ctagagctcc ttgtagaata tttccgacga tgcctgattg agatctttgg
     9301 cattttaaag gagtatgagg tgggtgaccc aggacagaga acgctactgg atcctgggag
     9361 gttcagcaag gtgtctagtc cagctcccat ggagggtggg gaagaagaag aagaacttct
     9421 aggtcctaaa ctagaagagg aagaagaaga ggaagtagtt gaaaatgatg aggagatagc
     9481 cttttcaggc aaggacaagc cagcttcaga gaatagtgag gagaagctga tcagtaagtt
     9541 tgacaagctt ccagtaaaga tcgtacagaa gaatgatcca tttgtggtgg actgctcaga
     9601 taagcttggg cgtgtgcagg agtttgacag tggcctgctg cactggcgga ttggtggggg
     9661 ggacaccact gagcatatcc agacccactt cgagagcaag acagagctgc tgccttcccg
     9721 gcctcacgca ccctgcccac cagcccctcg gaagcatgtg acaacagcag agggtacacc
     9781 agggacaaca gaccaggagg ggcccccacc tgatggacct ccagaaaaac ggatcacagc
     9841 cactatggat gacatgttgt ctactcggtc tagcaccttg accgaggatg gagctaagag
     9901 ttcagaggcc atcaaggaga gcagcaagtt tccatttggc attagcccag cacagagcca
     9961 ccggaacatc aagatcctag aggacgaacc ccacagtaag gatgagaccc cactgtgtac
    10021 ccttctggac tggcaggatt ctcttgccaa gcgctgcgtc tgtgtgtcca ataccattcg
    10081 aagcctgtca tttgtgccag gcaatgactt tgagatgtcc aaacacccag ggctgctgct
    10141 catcctgggc aagctgatcc tgctgcacca caagcaccca gaacggaagc aggcaccact
    10201 aacttatgaa aaggaggagg aacaggacca aggggtgagc tgcaacaaag tggagtggtg
    10261 gtgggactgc ttggagatgc tccgggaaaa caccttggtt acactcgcca acatctcggg
    10321 gcagttggac ctatctccat accccgagag catttgcctg cctgtcctgg acggactcct
    10381 acactgggca gtttgccctt cagctgaagc ccaggacccc ttttccaccc tgggccccaa
    10441 tgccgtcctt tccccgcaga gactggtctt ggaaaccctc agcaaactca gcatccagga
    10501 caacaatgtg gacctgattc tggccacacc ccccttcagc cgcctggaga agttgtatag
    10561 cactatggtg cgcttcctca gtgaccgaaa gaacccggtg tgccgggaga tggctgtggt
    10621 actgctggcc aacctggctc agggggacag cctggcagct cgtgccattg cagtgcagaa
    10681 gggcagtatc ggcaacctcc tgggcttcct agaggacagc cttgccgcca cacagttcca
    10741 gcagagccag gccagcctcc tccacatgca gaacccaccc tttgagccaa ctagtgtgga
    10801 catgatgcgg cgggctgccc gcgcgctgct tgccttggcc aaggtggacg agaaccactc
    10861 agagtttact ctgtacgaat cacggctgtt ggacatctcg gtatcaccgt tgatgaactc
    10921 attggtttca caagtcattt gtgatgtact gtttttgatt ggccagtcat agtaatgaac
    10981 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
    11041 ccgccggaat acgggagggt ctaaacgcgt taagtcgaca atcaacctct ggattacaaa
    11101 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
    11161 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
    11221 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
    11281 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
    11341 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
    11401 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
    11461 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
    11521 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
    11581 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
    11641 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
    11701 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
    11761 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
    11821 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
    11881 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
    11941 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
    12001 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
    12061 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    12121 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    12181 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
    12241 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
    12301 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
    12361 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
    12421 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
    12481 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
    12541 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
    12601 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
    12661 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
    12721 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
    12781 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    12841 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
    12901 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
    12961 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
    13021 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    13081 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    13141 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    13201 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    13261 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    13321 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    13381 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    13441 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    13501 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    13561 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    13621 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    13681 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    13741 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    13801 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    13861 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    13921 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    13981 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    14041 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    14101 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    14161 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    14221 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    14281 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    14341 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    14401 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    14461 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    14521 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    14581 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    14641 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    14701 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    14761 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    14821 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    14881 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    14941 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    15001 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    15061 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    15121 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
    15181 caattaaccc tcactaaagg gaacaaaagc tggagctgca agc
//
