LOCUS       sequence-400553-       12754 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12754)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2678
FEATURES             Location/Qualifiers
     source          1..12754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8611..9199
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8664..8684)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9082..9093)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9271..9504
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9582..9703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9619..9638)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9673..9692
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9743..9878
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9898..9917)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9899..9917)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9927..9944)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9927..9943)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9936..9958)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10085..10540
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10172..10191)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10382..10403
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10566..10670
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10671..10739,10740..11531)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10889..10908)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11702..12290
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12191..12210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12444..12461
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12578..12599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12614..12619,12620..12637,12638..12644)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12652..12668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12657..12679
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12676..12692
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12676..12692
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12711..12731
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12713..12731
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtac caaccagaag acgacaacaa
     4141 cagtgacgtg accagcgacg acgacatgac ccggaacaga agagagtcct caccacctca
     4201 ctcagtccat tctttcagtg accgggactg ggaccggagg ggcagaagca gagacatgga
     4261 gccacgagac cgctggtccc acaccaggaa cccaagaagc aggatgcctc cgcgggatct
     4321 ttcccttcct gtggtggcga aaacaagctt tgaaatggac agagaggacg acagggactc
     4381 cagggcttat gagtcccgat ctcaggatgc tgaatcatac caaaatgtgg tggacctcgc
     4441 tgaggacagg aaacctcaca acacaatcca ggacaacatg gaaaactaca ggaagctgct
     4501 ctccctcgtg cagcttgctg aagacgatgg ccactcccac atgacgcagg gccactcatc
     4561 aagatccaag agaagtgcct acccaagcac cagtcgaggt ctaaaaacta tgcctgaagc
     4621 caaaaaatca acccaccggc gggggatttg tgaagatgaa tcttcccacg gagtgataat
     4681 ggaaaaattc atcaaggatg tgtcacgcag ttccaaatcg ggaagagcaa gggagtcaag
     4741 cgaccggtca cagagattcc ccagaatgtc agatgataac tggaaggaca tttcattgaa
     4801 caagagggag tcagtgatcc agcagcgggt ttatgaaggg aatgcattta ggggaggctt
     4861 taggtttaat tcaacccttg tttccagaaa gagagttctt gaaagaaaga ggcgctatca
     4921 ttttgacaca gatgggaagg gctcgattca cgatcaaaaa ggctgtccca ggaagaagcc
     4981 ctttgaatgt ggtagtgaga tgagaaaagc catgagcgtg agcagcctga gcagcctcag
     5041 ctccccctcc tttaccgagt cacagccaat tgattttggg gcaatgccat atgtatgtga
     5101 tgagtgtggg aggtcgttca gtgtcatctc agaatttgtt gagcaccaga tcatgcatac
     5161 tagagagaac ctctatgagt atggtgagtc ctttatccac agtgtggctg tcagtgaagt
     5221 tcagaaaagt caggttggag ggaaacgttt tgaatgtaag gactgtggag agaccttcaa
     5281 taagagtgcc gccttggctg aacatcggaa gattcatgct agaggttatc ttgtggaatg
     5341 taagaatcag gaatgtgagg aagccttcat gcctagcccc acctttagtg agcttcagaa
     5401 aatatatggc aaagacaaat tctacgagtg cagggtgtgt aaggaaacct tccttcatag
     5461 ttctgccctg attgagcacc agaaaatcca ctttggggat gacaaagata atgagcgtga
     5521 acatgaacgt gaacgtgaac gtgagcgcgg ggaaaccttt aggcccagcc cagcccttaa
     5581 tgagtttcag aaaatgtatg gtaaagagaa aatgtacgaa tgtaaggtgt gtggggagac
     5641 tttccttcat agctcatccc tgaaagaaca tcagaaaatc catactagag ggaacccatt
     5701 tgaaaacaag ggtaaagtgt gtgaggaaac ctttattcct ggtcagtccc ttaaaaggcg
     5761 tcagaaaact tacaataagg agaagctctg tgactttaca gatggccggg atgccttcat
     5821 gcaaagctca gagctcagtg agcatcagaa aattcattct cgaaagaacc tctttgaagg
     5881 cagagggtat gagaaatctg tcattcatag tgggccattc actgaatctc agaagagtca
     5941 tactataaca agacctcttg aaagtgatga ggacgaaaag gcgttcacca ttagctctaa
     6001 cccctatgaa aaccagaaga ttcccactaa ggaaaatgtc tatgaggcaa aatcatatga
     6061 gaggtctgtt attcatagct tagcctctgt ggaagctcag aaaagtcaca gtgtagcagg
     6121 gcccagtaaa ccaaaagtaa tggcagagtc taccattcag agcttcgatg ctatcaacca
     6181 tcagagagtt cgtgctggag ggaacacctc tgaaggaagg gaatacagta ggtctgttat
     6241 ccatagctta gtggcttcca aacctccaag aagtcacaat ggaaatgaat tggtggaatc
     6301 taatgagaag ggagaatcct ccatttatat ctcagacctt aatgataagc gacagaagat
     6361 tcctgccaga gagaaccctt gtgaaggggg cagtaagaat cgcaactatg aagactctgt
     6421 catacagagt gtattccgtg ccaaacctca gaaaagtgtt cctggagagg gatctggtga
     6481 gtttaagaag gatggcgaat tctctgttcc cagctcaaat gtccgtgaat accagaaggc
     6541 tcgtgctaaa aagaaataca ttgagcatag gagcaatgag acctctgtaa ttcactctct
     6601 gccttttggt gaacaaacat ttcgccctcg agggatgctc tatgaatgtc aggagtgtgg
     6661 ggagtgcttt gctcatagct ctgacctcac tgagcaccag aagattcatg atagggagaa
     6721 gccctctgga agcagaaact atgaatggtc tgtcattcgc agcttggccc ctactgaccc
     6781 tcaaacaagt tacgcccaag agcagtatgc taaagagcaa gcgcggaaca aatgtaagga
     6841 cttcagacaa ttttttgcta ccagcgaaga cctcaacaca aaccagaaaa tctatgacca
     6901 agagaagtct catggcgagg agtctcaagg cgagaatact gatggggagg agacccacag
     6961 cgaggagacc catggtcagg agacaattga agaccctgtc attcaaggct cagacatgga
     7021 agaccctcag aaggatgacc ctgatgacaa aatctatgaa tgtgaggact gtggcctggg
     7081 ctttgtggat ctcacagacc tcacagacca tcagaaagtc cacagcagga agtgcctggt
     7141 tgacagtcgg gagtacacac attctgtaat tcacacccat tccatcagcg agtatcagag
     7201 agattacact ggagagcagc tgtatgaatg tccaaagtgt ggggaatctt ttattcatag
     7261 ctcattcctt ttcgagcatc agagaatcca tgaacaagac cagttgtatt ccatgaaggg
     7321 gtgtgatgat ggttttattg ccctcttgcc catgaagcca cggaggaatc gtgctgcaga
     7381 gaggaatcct gctcttgctg ggtcggccat tcgatgcctt ttgtgtggac aaggcttcat
     7441 tcatagctct gcccttaatg agcatatgag acttcatagg gaagatgatt tactggagca
     7501 gagccagatg gctgaggaag ctatcattcc aggcttagcc ctcactgagt ttcagagaag
     7561 tcagaccgaa gagagactct ttgaatgtgc agtctgtgga gaatctttcg tcaacccagc
     7621 agaacttgca gatcacgtaa ctgttcataa gaatgagccc tatgagtacg ggtcctccta
     7681 tactcacacc tcatttctta ctgagcccct caaaggagct ataccattct atgaatgcaa
     7741 ggattgtggt aagtccttta ttcatagcac agtcctcact aaacataagg agcttcatct
     7801 ggaagaagaa gaagaagatg aagcagcagc agctgcagca gcagcagccc aggaagttga
     7861 agccaatgtc catgttccac aagtagttct gaggattcag ggcttaaacg tagaggctgc
     7921 tgagccagaa gtggaggctg ccgagccaga agtggaggct gctgagccag aagtggaggc
     7981 tgctgagcca aacggagagg ctgaagggcc agatggagag gctgcagagc ccattggaga
     8041 ggctggacag ccaaatggag aggccgagca gccaaatggg gatgctgatg agccagatgg
     8101 tgcaggtatt gaagacccag aagaaagagc tgaagagcca gagggaaaag ctgaagagcc
     8161 agagggagat gccgacgagc ctgacggtgt gggaattgaa gacccagaag aaggtgaaga
     8221 tcaagagatt caggtagaag aaccatacta tgactgccat gaatgcacag aaaccttcac
     8281 ttccagcaca gcattcagtg aacacctgaa aactcatgcc agcatgatca tatttgagcc
     8341 tgcaaatgcc tttggggagt gctcaggcta catcgaacgt gccagcacca gcacaggtgg
     8401 tgccaatcaa gctgatgaga agtacttcaa atgtgacgtc tgtgggcagc tcttcaatga
     8461 ccgcctgtcc ctcgccagac accagaatac ccacactggc tagtaatgaa ctagtccgta
     8521 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg agcgtgcaat
     8581 aaacgacaag ctcggacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     8641 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     8701 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     8761 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     8821 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     8881 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     8941 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     9001 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     9061 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     9121 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     9181 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     9241 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     9301 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     9361 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     9421 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     9481 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     9541 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     9601 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     9661 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     9721 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9781 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     9841 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     9901 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     9961 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    10021 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
    10081 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
    10141 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
    10201 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
    10261 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
    10321 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
    10381 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
    10441 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
    10501 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
    10561 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    10621 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
    10681 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    10741 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    10801 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    10861 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
    10921 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    10981 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    11041 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    11101 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    11161 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    11221 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    11281 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    11341 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    11401 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    11461 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    11521 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    11581 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    11641 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    11701 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    11761 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    11821 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    11881 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    11941 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    12001 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    12061 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    12121 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    12181 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    12241 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    12301 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    12361 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    12421 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    12481 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    12541 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    12601 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    12661 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
    12721 ctcactaaag ggaacaaaag ctggagctgc aagc
//
