LOCUS       sequence-400557-       15886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15886)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 15886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2388
FEATURES             Location/Qualifiers
     source          1..15886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(11043..11057)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     misc_feature    11743..12331
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11796..11816)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12214..12225)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12403..12636
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12714..12835
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12751..12770)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12805..12824
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12875..13010
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(13030..13049)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13031..13049)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13059..13076)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13059..13075)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13068..13090)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13217..13672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13304..13323)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13514..13535
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13698..13802
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13803..13871,13872..14663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14021..14040)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14834..15422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15323..15342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15576..15593
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    15710..15731
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15746..15751,15752..15769,15770..15776)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15784..15800
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15789..15811
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15808..15824
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15808..15824
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     15843..15863
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        15845..15863
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag gtgcttcagt gtgatggctg
     4141 tgatttccga gccccgtctt atgaagatct caaggcacac attcaggatg tccacacggc
     4201 atttctgcag ccaactgatg ttgctgagga caatgtgaat gagctacgat gtgggtccgt
     4261 gaatgccagt aatcagacag aggtggagtt ttcttctata aaggatgaat ttgccattgc
     4321 agaagattta tcaggtcaaa atgcaacttc attggggacc ggaggttact atggccacag
     4381 tccaggatat tatggtcagc atattgccgc taatcccaaa ccaacaaaca agttttttca
     4441 atgcaagttc tgtgtacgct acttcaggtc aaaaaacctc ctcatagaac acactagaaa
     4501 ggtccatgga gctcaagctg aagggagttc atcaggaccc cctgtcccgg gatccttaaa
     4561 ttataatatc atgatgcacg agggatttgg aaaggtcttc tcttgccagt tttgcacata
     4621 caagtcacca agaagggcaa gaataattaa gcatcagaag atgtatcaca aaaacaattt
     4681 gaaggagacc actgctcccc cacctgctcc tgctccaatg ccagaccctg tggttccgcc
     4741 cgtatcactg caggacccct gcaaggaact gccagcagag gttgtggagc gcagcatctt
     4801 agagtctatg gtcaagcctt tgaccaaatc tcgaggcaac ttttgttgtg agtggtgcag
     4861 ctaccagacc ccccgccgag aacgctggtg tgaccacatg atgaagaaac accgcagtat
     4921 ggtcaagatc ctttccagtc tcagacagca acaagaagga actaatctac ctgatgtgcc
     4981 gaacaagagt gcccccagcc ccacttccaa ctccacctat ctgaccatga atgctgcaag
     5041 ccgggagata cccaatacta ccgtctccaa cttcaggggc tccatgggca actccatcat
     5101 gagacccaat tcttcagctt ccaagttttc gcccatgtct taccctcaga tgaagccgaa
     5161 gtcacctcac aattctggtc tagttaactt gacagagaga tcccgttatg gaatgactga
     5221 catgaccaat tcttctgctg acctggaaac taacagcatg ctaaatgact ctagttctga
     5281 tgaagagtta aatgaaatag acagtgagaa tggtttaagt gctatggatc accagacatc
     5341 aggcctgtct gcagagcagc tgatgggctc agatggcaac aaattattgg agaccaaggg
     5401 gattccattt agaagattca tgaataggtt ccagtgcccc ttttgtcctt tcctcaccat
     5461 gcatcgacgt agcatctctc gtcacataga aaacatccac ttatctggaa agacagctgt
     5521 ctacaaatgt gacgaatgtc cgtttacttg caagagctcg ttgaaacttg gggctcacaa
     5581 acagtgtcac acgggtacaa cgtcagattg ggatgctgtg aattcccaga gtgaaagcat
     5641 ttcttcctca ctgaatgaag gtgtggtgtc ttatgagagc tcaagcatca atggtagaaa
     5701 gtcaggagtc atgttggatc ccttgcagca gcaacagcca ccgcagccac caccaccgcc
     5761 gccgccacca ccaccatcac agccacagcc actgcagcag ccacagccac cacagctgca
     5821 gccaccacat caggtgccac cccagccaca aacacagcca ccaccaacgc agcagccaca
     5881 gccacccaca caagccgcac ctctgcaccc atacaaatgc accatgtgta attactccac
     5941 cacaactctg aaagggctaa gagtccatca gcagcataaa cattcattct gtgacaactt
     6001 gccaaaattc gaggggcagc cctcaagcct accattggaa aatgagacag acagccaccc
     6061 ctcttccagc aacactgtga agaaaagtca gacctcaatt cttgggttgt cctccaagaa
     6121 caattttgta gctaaagcct ctaggaagct cgccaatgac tttcctctag atttgtcacc
     6181 cgtgaagaag agaaccagga ttgacgagat agcaagcaac cttcagagca aaattaacca
     6241 aaccaaacag caggaagatg cagtgatcaa tgttgaggat gatgaagagg aagaggaaga
     6301 caacgaagtc gagatagagg ttgagttgga cagggaggaa gaaccgacag aacccatcat
     6361 agaggttccc acttcctttt ctgcccaaca gatatgggta agagatacca gtgagcccca
     6421 gaaagagccc aacttcagaa acatcaccca cgattacaat gccaccaatg gggctgagat
     6481 tgagctcacc ctttctgaag atgaagagga ttattatggc tcctcaacaa acttgaaaga
     6541 tcaccaagtt tccaatactg ctctgctgaa tacccaaact cccatctatg ggactgagca
     6601 caatagtgaa aacacagact ttggtgactc tggaaggctt tactattgta aacactgtga
     6661 ctttaacaac aaatctgccc ggagtgttag cacccactac caacgaatgc acccatacat
     6721 taaattcagc tttaggtaca tcttggaccc caatgatcac agtgcagtgt acaggtgcct
     6781 ggaatgctac atcgattaca ccaacttcga agatctccag cagcattatg gcgagcacca
     6841 cccagaagcc atgaatgtac tcaactttga tcactcggac ctgatctacc ggtgtcggtt
     6901 ttgttcatac acgagcccga atgttagaag cctgatgcca cattaccaaa gaatgcatcc
     6961 cacggtgaag atcaacaacg cgatgatatt ttcaagctat gtcgtggagc agcaggaagg
     7021 gctgaataca gaatcccaga ccctgaggga gattctgaat tcggctccca agaacatggc
     7081 gacttccaca cctgtggctc gtggtggtgg tttgccagct acgttcaaca aaaacactcc
     7141 taagaccttt actcctgaat gtgaaaatca gaaggaccct ttggtcaaca ctgttgttgt
     7201 ttatgattgt gatgtttgtt cgtttgcaag ccccaacatg cattctgtct tggttcatta
     7261 tcagaagaaa caccccgaag aaaaggcttc ctactttagg atccagaaaa ctatgcgaat
     7321 ggtgtctgtg gacaggggct ctgccctttc tcaattatca tttgaggtgg gtgctccaat
     7381 gtctcccaaa atgtccaaca tgggttcccc acccccccca caacccccgc caccagacct
     7441 cagtactgag ctttactact gcaaacactg ttcctacagc aatcggtcag ttgtgggagt
     7501 gcttgtccac taccagaaaa gacacccaga aataaaggtt actgccaaat atatcagaca
     7561 ggctcctccc acagctgcaa tgatgagagg ggtcgaaggg ccccaaggct ccccccggcc
     7621 acccgccccc atacaacagc tgaaccgaag cagctctgag agagatggcc ctcctgtgga
     7681 gaatgagatg ttcttttgcc agcactgtga ttatgggaac cggacggtca aaggggtact
     7741 cattcattat cagaagaagc accgagactt caaggccaat gcagatgtga tccggcagca
     7801 tacggccacc attcgaagcc tctgcgaccg aaatcagaag aagcctgcca gctgcgtgct
     7861 tgtctccccc tctaatctgg agcgggacaa aacgaaactc cgagcactca aatgtaggca
     7921 gtgctcatat acctccccct acttctatgc actgaggaag catatcaaga aagaccaccc
     7981 cgccctgaaa gccacagtca cgtccatcat gcgatgggca tttctagatg gcttgataga
     8041 agctggctac cactgcgagt ggtgcatcta ctcccatacg gagcccaacg gtttgctcct
     8101 gcattaccaa cggaggcatc cagaacacta tgttgattac acctacatgg ctactaaact
     8161 gtgggctggg ccagacccat cccctccctc tctcacaatg ccagccgaag ccaaaaccta
     8221 cagatgcagg gactgtgttt tcgaagctgt ttccatctgg gacatcacta atcactacca
     8281 agcattccac ccctgggcca tgaatggtga tgagtcagtg ctactggaca tcatcaagga
     8341 gaaagatgct gtggagaagc ccattctttc atccgaagag ttggcaggcc ctgtgaattg
     8401 tgaaaacagt atacccaccc ctttcccgga gcaggaagct gaatgtccag aggatgcaag
     8461 actgtcccct gagaaaagcc tgcagctagc ttcagccaac cccgccatat cctccacccc
     8521 ataccagtgc acggtatgcc aatctgagta taacaacttg cacggccttc tcactcatta
     8581 tgggaagaag caccctggca tgaaagtgaa ggctgctgac tttgcccagg acattgacat
     8641 caacccaggt gccgtctaca aatgcaggca ttgcccatac atcaacaccc gcatccacgg
     8701 cgtactgacc cactaccaga agcgacaccc gtccatcaag gtgaccgctg aggactttgt
     8761 gcacgacgta gagcagtctg ctgacatatc ccagaatgac gtggaggaga cgagcaggat
     8821 cttcaagcaa gggtatggcg cctaccggtg caaactgtgt ccgtacacac acggcacttt
     8881 ggagaaacta aaaatccact acgagaagta tcacaatcag cctgaatttg atgtcttttc
     8941 ccagtcgccc ccgaagctgc cagtccccct cgagcccgag atgaccactg aagtgagccc
     9001 ttcccaagtc tccatcactg aggaggaggt gggagaggag cccgtgtcca cttctcactt
     9061 ctctacctcc cacctggtct cccacactgt gttccggtgc cagctctgca agtacttctg
     9121 ctccacgagg aaggggatcg ccaggcacta ccgcatcaag cacaataatg tccgagccca
     9181 gccagaaggc aagaacaacc tcttcaagtg tgccctgtgt gcctacacca accccatccg
     9241 caaaggtctg gcagcccact accagaagcg ccacgacatt gatgcgtatt acactcactg
     9301 cttggcagcc tccaggacca tcagcgacaa gcccaacaaa gtgatcatcc catccccgcc
     9361 caaggacgac tcccctcagc tgagcgagga actccggcgg gcagtggaga agaaaaagtg
     9421 ctccttgtgc tctttccagt cgttcagcaa gaagggcatc gtgtcccatt acatgaaacg
     9481 ccacccaggg gtgttcccaa agaagcagca cgccagcaag ttggggggct acttcacggc
     9541 cgtctatgca gatgagcatg agaagcccac actgatggaa gaagaggaga gaggcaactt
     9601 tgagaaagcc gaggtggagg gtgaagctca ggaaatcgag tggctcccat tccgctgcat
     9661 caaatgcttc aagctgtcct ttagcactgc agagctgctg tgcatgcatt acactgacca
     9721 ccacagtcgg gacctaaaga gggacttcat cattctgggc aacggccccc gcttgcagaa
     9781 ctccacctac cagtgtaagc actgtgatag caaactgcaa agcacagccg agctgacctc
     9841 acacttgaac attcacaatg aggaattcca gaagcgtgcc aaacgtcagg agaggaggaa
     9901 acagcttttg agcaagcaga aatatgcaga tggtgctttt gcagatttca aacaagagag
     9961 gccttttggt cacttagaag aggtgccaaa gatcaaggag aggaaagtgg tgggctacaa
    10021 atgtaaattc tgtgtggaag tgcacccaac gctccgagcc atctgcaatc acctccgaaa
    10081 gcacgtccag tatggcaatg tcccagctgt gtcagctgct gtgaaggggc tgcgttctca
    10141 tgagaggagc cacctggccc tggccatgtt tacccgcgag gacaagtaca gctgccagta
    10201 ttgctcgttt gtttctgctt tcaggcacaa tttggatcgc catatgcaaa cccaccacgg
    10261 acaccataaa ccattccgat gcaaactctg ctccttcaag tcctcctata acagccggct
    10321 gaaaacacat atactcaaag ctcatgctgg tgagcatgcc tacaagtgtt cttggtgctc
    10381 attctccacc atgacaatca gccagctgaa ggaacactcc ctcaaggtcc acggaaaagc
    10441 cctgaccctc cccaggccac ggatcgtcag tctcctctcc tcacactccc accactcctc
    10501 ccaaaaagct accccggctg aagaagtgga agactccaat gactcatcat attcagagcc
    10561 cccagatgtt cagcagcagt tgaaccacta tcagtcagct gccctggcaa ggaacaacag
    10621 ccgtgttagc cctgtgcctc tttctggggc tgctgctggc actgagcaga aaactgaagc
    10681 cgtgcttcac tgcgaattct gtgaattctc ctccggctac atccagagca tcaggcgtca
    10741 ttaccgggac aagcatggtg ggaagaagct tttcaagtgc aaagactgct ccttttacac
    10801 aggctttaaa tctgctttta ctatgcacgt ggaagctggg cactcagcag ttcccgagga
    10861 gggccccaaa gatcttcgct gtcctctctg cctctatcac accaaataca agcgcaacat
    10921 gattgaccac atcgtgctgc accgagaaga gcgtgttgtc cccattgaag tttgccggtc
    10981 caaactgtcc aaatacttgc agggagtagt tttccgctgt gataagtgta ccttcacctg
    11041 ctccagtgat gagagcctcc agcaacatat agaaaagcac aatgaactga aaccttacaa
    11101 atgccagctc tgctactatg agaccaagca cacggaggaa ctggacagcc accttcggga
    11161 tgagcataag gtaagccgta actttgagct ggttggacgg gttaacttgg atcagctgga
    11221 acagatgaag gagaaaatgg agagctccag cagcgatgat gaggacaagg aagaagaaat
    11281 gaacagcaag gctgaagaca gagagctgat gagattttct gaccacgggg ctgctcttaa
    11341 cactgagaag cgttttccat gtgaattttg tggacgggcg ttttcacagg gctctgagtg
    11401 ggaaagacat gtgctgagac acggcatggc attgaatgac accaagcagg tgagcagaga
    11461 agaaatccac ccaaaagaga tcatggagaa cagtgttaaa atgccctcca tagaggaaaa
    11521 ggaagatgac gaggccattg ggatagactt ttccctaaag aatgaaacag tagccatctg
    11581 tgtagtaact gccgacaaat ctctcctgga gaatgcagag gccaaaaaag aatagtaatg
    11641 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
    11701 cgaggattgc tagctcgcta tgccgggacg cgttaagtcg acaatcaacc tctggattac
    11761 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
    11821 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
    11881 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
    11941 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
    12001 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
    12061 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
    12121 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
    12181 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
    12241 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
    12301 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
    12361 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
    12421 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
    12481 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
    12541 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
    12601 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
    12661 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
    12721 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    12781 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    12841 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
    12901 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    12961 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
    13021 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
    13081 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
    13141 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    13201 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    13261 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    13321 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    13381 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    13441 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    13501 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    13561 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    13621 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
    13681 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    13741 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    13801 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    13861 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    13921 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    13981 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    14041 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    14101 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    14161 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    14221 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    14281 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    14341 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    14401 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    14461 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    14521 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    14581 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    14641 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    14701 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    14761 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    14821 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    14881 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    14941 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    15001 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    15061 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    15121 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    15181 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    15241 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    15301 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    15361 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    15421 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    15481 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    15541 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    15601 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    15661 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    15721 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    15781 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
    15841 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagc
//
