LOCUS       sequence-400560-       14572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14572)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 14572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2433
FEATURES             Location/Qualifiers
     source          1..14572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             6895..6903
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    10429..11017
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10482..10502)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10900..10911)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11089..11322
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11400..11521
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11437..11456)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11491..11510
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11561..11696
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11716..11735)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11717..11735)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11745..11762)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11745..11761)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11754..11776)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11903..12358
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11990..12009)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12200..12221
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12384..12488
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12489..12557,12558..13349)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12707..12726)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13520..14108
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14009..14028
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14262..14279
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14396..14417
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14432..14437,14438..14455,14456..14462)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14470..14486
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14475..14497
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14494..14510
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14494..14510
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14529..14549
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14531..14549
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcc gcgcaggtcg cccccgccgc
     4141 cgccagcagc ctgggcaacc cgccgccgcc gccgccctcg gagctgaaga aagccgagca
     4201 gcagcagcgg gaggaggcgg ggggcgaggc ggcggcggcg gcagcggccg agcgcgggga
     4261 aatgaaggca gccgccgggc aggaaagcga gggccccgcc gtggggccgc cgcagccgct
     4321 gggaaaggag ctgcaggacg gggccgagag caatgggggt ggcggcggcg gcggagccgg
     4381 cagcggcggc gggcccggcg cggagccgga cctgaagaac tcgaacggga acgcgggccc
     4441 taggcccgcc ctgaacaata acctcacgga gccgcccggc ggcggcggtg gcggcagcag
     4501 cgatggggtg ggggcgcctc ctcactcagc cgcggccgcc ttgccgcccc cagcctacgg
     4561 cttcgggcaa ccctacggcc ggagcccgtc tgccgtcgcc gccgccgcgg ccgccgtctt
     4621 ccaccaacaa catggcggac aacaaagccc tggcctggca gcgctgcaga gcggcggcgg
     4681 cgggggcctg gagccctacg cggggcccca gcagaactct cacgaccacg gcttccccaa
     4741 ccaccagtac aactcctact accccaaccg cagcgcctac cccccgcccg ccccggccta
     4801 cgcgctgagc tccccgagag gtggcactcc gggctccggc gcggcggcgg ctgccggctc
     4861 caagccgcct ccctcctcca gcgcctccgc ctcctcgtcg tcttcgtcct tcgctcagca
     4921 gcgcttcggg gccatggggg gaggcggccc ctccgcggcc ggcgggggaa ctccccagcc
     4981 caccgccacc cccaccctca accaactgct cacgtcgccc agctcggccc ggggctacca
     5041 gggctacccc gggggcgact acagtggcgg gccccaggac gggggcgccg gcaagggccc
     5101 ggcggacatg gcctcgcagt gttggggggc tgcggcggcg gcagctgcgg cggcggccgc
     5161 ctcgggaggg gcccaacaaa ggagccacca cgcgcccatg agccccggga gcagcggcgg
     5221 cggggggcag ccgctcgccc ggacccctca gccatccagt ccaatggatc agatgggcaa
     5281 gatgagacct cagccatatg gcgggactaa cccatactcg cagcaacagg gacctccgtc
     5341 aggaccgcag caaggacatg ggtacccagg gcagccatac gggtcccaga ccccgcagcg
     5401 gtacccgatg accatgcagg gccgggcgca gagtgccatg ggcggcctct cttatacaca
     5461 gcagattcct ccttatggac aacaaggccc cagcgggtat ggtcaacagg gccagactcc
     5521 atattacaac cagcaaagtc ctcaccctca gcagcagcag ccaccctact cccagcaacc
     5581 accgtcccag acccctcatg cccaaccttc gtatcagcag cagccacagt ctcaaccacc
     5641 acagctccag tcctctcagc ctccatactc ccagcagcca tcccagcctc cacatcagca
     5701 gtccccggct ccatacccct cccagcagtc gacgacacag cagcaccccc agagccagcc
     5761 cccctactca cagccacagg ctcagtctcc ttaccagcag cagcaacctc agcagccagc
     5821 accctcgacg ctctcccagc aggctgcgta tcctcagccc cagtctcagc agtcccagca
     5881 aactgcctat tcccagcagc gcttccctcc accgcaggag ctatctcaag attcatttgg
     5941 gtctcaggca tcctcagccc cctcaatgac ctccagtaag ggagggcaag aagatatgaa
     6001 cctgagcctt cagtcaagac cctccagctt gcctgatcta tctggttcaa tagatgacct
     6061 ccccatgggg acagaaggag ctctgagtcc tggagtgagc acatcaggga tttccagcag
     6121 ccaaggagag cagagtaatc cagctcagtc tcctttctct cctcatacct cccctcacct
     6181 gcctggcatc cgaggccctt ccccgtcccc tgttggctct cccgccagtg ttgctcagtc
     6241 tcgctcagga ccactctcgc ctgctgcagt gccaggcaac cagatgccac ctcggccacc
     6301 cagtggccag tcggacagca tcatgcatcc ttccatgaac caatcaagca ttgcccaaga
     6361 tcgaggttat atgcagagga acccccagat gccccagtac agttcccccc agcccggctc
     6421 agccttatct ccgcgtcagc cttccggagg acagatacac acaggcatgg gctcctacca
     6481 gcagaactcc atggggagct atggtcccca ggggggtcag tatggcccac aaggtggcta
     6541 ccccaggcag ccaaactata atgccttgcc caatgccaac taccccagtg caggcatggc
     6601 tggaggcata aaccccatgg gtgccggagg tcaaatgcat ggacagcctg gcatcccacc
     6661 ttatggcaca ctccctccag ggaggatgag tcacgcctcc atgggcaacc ggccttatgg
     6721 ccctaacatg gccaatatgc cacctcaggt tgggtcaggg atgtgtcccc caccaggggg
     6781 catgaaccgg aaaacccaag aaactgctgt cgccatgcat gttgctgcca actctatcca
     6841 aaacaggccg ccaggctacc ccaatatgaa tcaagggggc atgatgggaa ctggacctcc
     6901 ttatggacaa gggattaata gtatggctgg catgatcaac cctcagggac ccccatattc
     6961 catgggtgga accatggcca acaattctgc agggatggca gccagcccag agatgatggg
     7021 ccttggggat gtaaagttaa ctccagccac caaaatgaac aacaaggcag atgggacacc
     7081 caagacagaa tccaaatcca agaaatccag ttcttctact acaaccaatg agaagatcac
     7141 caagttgtat gagctgggtg gtgagcctga gaggaagatg tgggtggacc gttatctggc
     7201 cttcactgag gagaaggcca tgggcatgac aaatctgcct gctgtgggta ggaaacctct
     7261 ggacctctat cgcctctatg tgtctgtgaa ggagattggt ggattgactc aggtcaacaa
     7321 gaacaaaaaa tggcgggaac ttgcaaccaa cctcaatgtg ggcacatcaa gcagtgctgc
     7381 cagctccttg aaaaagcagt atatccagtg tctctatgcc tttgaatgca agattgaacg
     7441 gggagaagac cctcccccag acatctttgc agctgctgat tccaagaagt cccagcccaa
     7501 gatccagcct ccctctcctg cgggatcagg atctatgcag gggccccaga ctccccagtc
     7561 aaccagcagt tccatggcag aaggaggaga cttaaagcca ccaactccag catccacacc
     7621 acacagtcag atccccccat tgccaggcat gagcaggagc aattcagttg ggatccagga
     7681 tgcctttaat gatggaagtg actccacatt ccagaagcgg aattccatga ctccaaaccc
     7741 tgggtatcag cccagtatga atacctctga catgatgggg cgcatgtcct atgagccaaa
     7801 taaggatcct tatggcagca tgaggaaagc tccagggagt gatcccttca tgtcctcagg
     7861 gcagggcccc aacggcggga tgggtgaccc ctacagtcgt gctgccggcc ctgggctagg
     7921 aaatgtggcg atgggaccac gacagcacta tccctatgga ggtccttatg acagagtgag
     7981 gacggagcct ggaatagggc ctgagggaaa catgagcact ggggccccac agccgaatct
     8041 catgccttcc aacccagact cggggatgta ttctcctagc cgctaccccc cgcagcagca
     8101 gcagcagcag cagcaacgac atgattccta tggcaatcag ttctccaccc aaggcacccc
     8161 ttctggcagc cccttcccca gccagcagac tacaatgtat caacagcaac agcaggtatc
     8221 cagccctgct cccctgcccc ggccaatgga gaaccgcacc tctcctagca agtctccatt
     8281 cctgcactct gggatgaaaa tgcagaaggc aggtccccca gtacctgcct cgcacatagc
     8341 acctgcccct gtgcagcccc ccatgattcg gcgggatatc accttcccac ctggctctgt
     8401 tgaagccaca cagcctgtgt tgaagcagag gaggcggctc acaatgaaag acattggaac
     8461 cccggaggca tggcgggtaa tgatgtccct caagtctggt ctcctggcag agagcacatg
     8521 ggcattagat accatcaaca tcctgctgta tgatgacaac agcatcatga ccttcaacct
     8581 cagtcagctc ccagggttgc tagagctcct tgtagaatat ttccgacgat gcctgattga
     8641 gatctttggc attttaaagg agtatgaggt gggtgaccca ggacagagaa cgctactgga
     8701 tcctgggagg ttcagcaagg tgtctagtcc agctcccatg gagggtgggg aagaagaaga
     8761 agaacttcta ggtcctaaac tagaagagga agaagaagag gaagtagttg aaaatgatga
     8821 ggagatagcc ttttcaggca aggacaagcc agcttcagag aatagtgagg agaagctgat
     8881 cagtaagttt gacaagcttc cagtaaagat cgtacagaag aatgatccat ttgtggtgga
     8941 ctgctcagat aagcttgggc gtgtgcagga gtttgacagt ggcctgctgc actggcggat
     9001 tggtgggggg gacaccactg agcatatcca gacccacttc gagagcaaga cagagctgct
     9061 gccttcccgg cctcacgcac cctgcccacc agcccctcgg aagcatgtga caacagcaga
     9121 gggtacacca gggacaacag accaggaggg gcccccacct gatggacctc cagaaaaacg
     9181 gatcacagcc actatggatg acatgttgtc tactcggtct agcaccttga ccgaggatgg
     9241 agctaagagt tcagaggcca tcaaggagag cagcaagttt ccatttggca ttagcccagc
     9301 acagagccac cggaacatca agatcctaga ggacgaaccc cacagtaagg atgagacccc
     9361 actgtgtacc cttctggact ggcaggattc tcttgccaag cgctgcgtct gtgtgtccaa
     9421 taccattcga agcctgtcat ttgtgccagg caatgacttt gagatgtcca aacacccagg
     9481 gctgctgctc atcctgggca agctgatcct gctgcaccac aagcacccag aacggaagca
     9541 ggcaccacta acttatgaaa aggaggagga acaggaccaa ggggtgagct gcaacaaagt
     9601 ggagtggtgg tgggactgct tggagatgct ccgggaaaac accttggtta cactcgccaa
     9661 catctcgggg cagttggacc tatctccata ccccgagagc atttgcctgc ctgtcctgga
     9721 cggactccta cactgggcag tttgcccttc agctgaagcc caggacccct tttccaccct
     9781 gggccccaat gccgtccttt ccccgcagag actggtcttg gaaaccctca gcaaactcag
     9841 catccaggac aacaatgtgg acctgattct ggccacaccc cccttcagcc gcctggagaa
     9901 gttgtatagc actatggtgc gcttcctcag tgaccgaaag aacccggtgt gccgggagat
     9961 ggctgtggta ctgctggcca acctggctca gggggacagc ctggcagctc gtgccattgc
    10021 agtgcagaag ggcagtatcg gcaacctcct gggcttccta gaggacagcc ttgccgccac
    10081 acagttccag cagagccagg ccagcctcct ccacatgcag aacccaccct ttgagccaac
    10141 tagtgtggac atgatgcggc gggctgcccg cgcgctgctt gccttggcca aggtggacga
    10201 gaaccactca gagtttactc tgtacgaatc acggctgttg gacatctcgg tatcaccgtt
    10261 gatgaactca ttggtttcac aagtcatttg tgatgtactg tttttgattg gccagtcata
    10321 gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
    10381 aaaggacgag gagatagaag ctagggcgtt tatacgcgtt aagtcgacaa tcaacctctg
    10441 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
    10501 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
    10561 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
    10621 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
    10681 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
    10741 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
    10801 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
    10861 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
    10921 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
    10981 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
    11041 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
    11101 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
    11161 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
    11221 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
    11281 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
    11341 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
    11401 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    11461 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    11521 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
    11581 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
    11641 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    11701 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
    11761 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
    11821 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
    11881 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
    11941 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
    12001 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
    12061 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
    12121 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
    12181 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
    12241 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
    12301 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
    12361 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    12421 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    12481 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    12541 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    12601 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    12661 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    12721 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    12781 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    12841 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    12901 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    12961 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    13021 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    13081 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    13141 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    13201 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    13261 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    13321 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    13381 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    13441 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    13501 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    13561 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    13621 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    13681 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    13741 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    13801 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    13861 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    13921 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    13981 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    14041 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    14101 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    14161 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    14221 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    14281 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    14341 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    14401 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    14461 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    14521 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gc
//
