LOCUS       sequence-400572-        9135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of evoCjCas9 TadCDd.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Schmidheini L, Mathis N, Marquart KF, Rothgangl T, Kissling L, Bock 
            D, Chanez C, Wang JP, Jinek M, Schwank G
  TITLE     Continuous directed evolution of a compact CjCas9 variant with broad
            PAM compatibility.
  JOURNAL   Nat Chem Biol. 2023 Sep 21. doi: 10.1038/s41589-023-01427-x.
  PUBMED    37735239
REFERENCE   2  (bases 1 to 9135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2023 Sep 21. doi: 10.1038/s41589-023-01427-x."
COMMENT     Sequence Label: p2T-CMV-evoCjCas9-TadCDd-BlastR
FEATURES             Location/Qualifiers
     source          1..9135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             116..136
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3710..3958
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             3989..4237
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             4280..4300
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4310..4327
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4350..4367)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4356..4580
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4651..4667)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4651..4667)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4664..4686)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4675..4691
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4699..4705,4706..4723,4724..4729))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4744..4765
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4882..5003
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4893..4912)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4947..4966
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5161..5559)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(5578..5625)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(5673..6002)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5687..5822
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5741..5760)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     5987..6007
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    join(6126..6313,6314..6325)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     rep_origin      complement(6386..6974)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6466..6485)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7145..7936,7937..8005))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7768..7787
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8006..8110)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8189..8208)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(8400..8411,8412..8549)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     enhancer        8558..8937
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(8938..9135,1..6)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9092..9112
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agagctggtt tagtgaaccg tcagatccgc tagagatccg cggccgctaa tacgactcac
       61 tatagggaga gccgccacca tgaaacggac agccgacgga agcgagttcg agtcaccaaa
      121 gaagaagcgg aaagtctctg aggtggagtt ttcccacgag tactggatga gacatgccct
      181 gaccctggcc aagagggcac gggatgagag gaaggcccct gtgggagccg tgctggtgct
      241 gaacaataga gtgatcggcg agggctggaa cagagccatc ggcctgcacg acccaacagc
      301 ccatgccgaa attatcgccc tgagacaggg cggcctggtc atgcagaact acagactgat
      361 tgacgccacc ctgtacgtga cattcgagcc ttgcgtgatg tgcgccggcg ccatgatcaa
      421 ctctaggatc ggccgcgtgg tgtttggcgt gaggaactca aaaagaggcg ccgcaggctc
      481 cctgatgaac gtgctgaact accccggcat gaatcaccgc gtcgaaatta ccgagggaat
      541 cctggcagat gaatgtgccg ccctgctgtg cgatttctat cggatgccta gacaggtgtt
      601 caatgctcag aagaaggccc agagctccat caactccgga ggatctagcg gaggctcctc
      661 tggctctgag acacctggca caagcgagag cgcaacacct gaaagcagcg ggggcagcag
      721 cggggggtca gcccgcatcc tggcctttgc catcggcatc agcagcatcg gatgggcctt
      781 cagcgagaat gacgagctga aggactgcgg tgtgagaatc tttacaaagg tcgagaaccc
      841 caagaccggc gaaagcctgg cgctgccaag acggctggcc cggtctgctc ggaagagata
      901 cgcccggaga aaagccagac tgaatcacct gaaacacctg atcgccaacg agttcaaact
      961 gaactacgag gactaccaga gcttcgatga gagcttggct aaggcctaca aaggcagcct
     1021 gatcagcccc tacgagctga gattcagagc tctgaatgag ctcctgagca agcaggactt
     1081 cgctagagtg atcctgcaca tcgccaagcg aagaggctac gacgacatca agaactctga
     1141 tgacaaggag aagggcgcca ttctgaaagc catcaagcag aacgaagaga aactggccaa
     1201 ttaccagagc gtgggcgagt acctgtacaa ggagtacttc cagaagttca aagaaaatag
     1261 caaagagttc accaacgtgc ggaacaagaa ggagtcttat gaaagatgta tcgcccagag
     1321 cttcctgaaa gacgagctga aactgatctt caagaagcaa agagagtttg gcttcagctt
     1381 cagcaaaaaa tttgaagaag aggtgctgtc tgtcgccttc tacaagaggg ctctgaagga
     1441 cttcagccac ctggtgggca attgcagctt tttcacagac gaaaagcggg cccctaagaa
     1501 cagccctctg gccttcatgt tcgtggctct gaccagaatc atcaacctgc tgaacaacct
     1561 gaaaaatacc gagggcatcc tctataccaa ggacgatctg aacgccctgc tgaatgaggt
     1621 gctcaagaat ggcaccctga cctacaagca aacaaaaaaa ctgctgggcc tgtctgacga
     1681 ctacgagttt aaaggcgaga agggcaccta tttcatcgaa tttaagaagt acaaggaatt
     1741 catcaaggca ctgggcgaac acaacctgtc tcaggacgac ctgaacgaga tcgccaagga
     1801 catcaccctg atcaaggatg agatcaagct gaaaaaagct ctggccaagt acgacctgaa
     1861 tcaaaaccag atcgacagct taagcaagct ggaatttaag gatcacctga acatctcctt
     1921 taaggccctg aagctggtga ccccactgat gctggaagga aagaagtacg acgaagcatg
     1981 caacgagctt aacctgaagg ttgctatcaa cgaggataag aaggatttcc tgcctgcctt
     2041 taacgagaca tactacaagg atgaggtgac caaccccgtg gtgctgagag ctatcaaaga
     2101 gtacagaaag gtgctgaacg ccctgctgaa gaagtacggc aaggtccaca agatcaatat
     2161 tgagctggcc cgggaggttg gaaagaacca ctctcagaga gcaaagatcg agaaggagca
     2221 aaacgagaac tacaaagcga agaaggacgc cgaactggag tgcgagaagc ttggcctgaa
     2281 gatcaactct aagaatatcc tgaaactcag acttttcaaa gaacagaagg aattctgtgc
     2341 ctacagcggc gagaagatca aaatttctga cctgcaggat gaaaagatgc tggagatcga
     2401 ccacatctac ccttactcca gaagcttcga cgacagctat atgaacaaag tgctggtgtt
     2461 cacaaagcag aaccaggaga agctgaatca gacccctttc gaggccttcg gcaatgactc
     2521 cgccaagtgg cagaaaatcg aggtgctggc caaaaacctg ccaaccaaga aacagaagag
     2581 aatcctcgac aagaactaca aggacaagga acagaagaac ttcaaggatc ggaacctgaa
     2641 cgacacccgg tacatcgcca ggctggtgtt aaattacacc aaggactacc tggatttcct
     2701 gcccctgagc gacgacgaga acaccaagct gaacgacaca cagaagggca gcaaggtgca
     2761 cgtggaagcc aagagcggca tgctgaccag cgcactgcgc cacacttggg gcttcagcgc
     2821 taaggaccgg aacaaccacc tgcatcacgc catcgatgcc gtgatcatag cctacgccaa
     2881 caactcaatc gtgaaagctt tcagtgactt taagaaagaa caggagagca actctgccga
     2941 actgtacgcc aagaaaatta gcgagctgga ctacaagaac aaacggaagt tcttcgaacc
     3001 tttctcagga tttagacaga aggtgctgga taagatcgat gaaatcttcg tgtccaagcc
     3061 cgagagaaag aagcctagcg gagccctgca caaggaaacc ttcagaaagg aagaagagtt
     3121 ctaccagtct tatgggggca aagaaggcgt gctgaaggcc ctggaactcg gcaagatcag
     3181 aaaggtgaag ggaaagattg tgaagaacgg cgacatgttc agagtggaca tcttcaagca
     3241 caagaagacc aacaagttct atgccgtgcc tatctacaca atggatttcg ccctgaaagt
     3301 gctgcctaac aaggctgtcg ccagatctaa gaagggcgag atcaaggact ggatcctgat
     3361 ggacgaaaac tacgagttct gcttcagcct gtacaaggac agcctgatcc tgatccaaac
     3421 aaagaagatg caggagcctg aattcgtgta ctacaacgcc ttcacaagca gcaccgtgag
     3481 cctgatcgtg tctaaacatg ataacaagtt cgaaaccctg tccaagaacc aaaagatcct
     3541 gttcaagaac gccaacgaga aggaagtgat cgccaagggc atcggcattc agaatctgaa
     3601 ggtgttcgag aaatatatcg tgtccgctct gggagaggtt acaaaggccg agtttcggca
     3661 gagagaagat tttaagaaga gcggcgggag cggcgggagc ggggggagca ctaatctgag
     3721 cgacatcatt gagaaggaga ctgggaaaca gctggtcatt caggagtcca tcctgatgct
     3781 gcctgaggag gtggaggaag tgatcggcaa caagccagag tctgacatcc tggtgcacac
     3841 cgcctacgac gagtccacag atgagaatgt gatgctgctg acctctgacg cccccgagta
     3901 taagccttgg gccctggtca tccaggattc taacggcgag aataagatca agatgctgag
     3961 cggaggatcc ggaggatctg gaggcagcac caacctgtct gacatcatcg agaaggagac
     4021 aggcaagcag ctggtcatcc aggagagcat cctgatgctg cccgaagaag tcgaagaagt
     4081 gatcggaaac aagcctgaga gcgatatcct ggtccatacc gcctacgacg agagtaccga
     4141 cgaaaatgtg atgctgctga catccgacgc cccagagtat aagccctggg ctctggtcat
     4201 ccaggattcc aacggagaga acaaaatcaa aatgctgtct ggcggctcaa aaagaaccgc
     4261 cgacggcagc gaattcgagc ccaagaagaa gaggaaagtc taaaccggtc atcatcacca
     4321 tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     4381 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     4441 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     4501 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     4561 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct cgataccgtc
     4621 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     4681 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctagggtgc
     4741 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     4801 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     4861 tattgggcgc tacagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     4921 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     4981 ttataagctg caataaacaa gttggggtgg gcgaagaact ccagcatgag atccccgcgc
     5041 tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag
     5101 gcggcggtgg aatcgaaatc tcgtagcacg tgtcagtcct gctcctcggc cacgaagtgc
     5161 ttagccctcc cacacataac cagagggcag caattcacga atcccaactg ccgtcggctg
     5221 tccatcactg tccttcacta tggctttgat cccaggatgc agatcgagaa gcacctgtcg
     5281 gcaccgtccg caggggctca agatgcccct gttctcattt ccgatcgcga cgatacaagt
     5341 caggttgcca gctgccgcag cagcagcagt gcccagcacc acgagttctg cacaaggtcc
     5401 cccagtaaaa tgatatacat tgacaccagt gaagatgcgg ccgtcgctag agagagctgc
     5461 gctggcgacg ctgtagtctt cagagatggg gatgctgttg attgtagccg ttgctctttc
     5521 aatgagggtg gattcttctt gagacaaagg cttggccatg gtttagttcc tcaccttgtc
     5581 gtattatact atgccgatat actatgccga tgattaattg tcaacacgtg ctgatcagat
     5641 ccgaaaatgg atatacaagc tcccgggagc tttttgcaaa agcctaggcc tccaaaaaag
     5701 cctcctcact acttctggaa tagctcagag gcagaggcgg cctcggcctc tgcataaata
     5761 aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg
     5821 atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat gctttgcata
     5881 cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc
     5941 atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac
     6001 acattccaca gaattcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6061 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6121 catgtaagtg atctccaaaa aataagtact ttttgactgt aaataaaatt gtaaggagta
     6181 aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa
     6241 ttgtactcaa gtaaagtaaa aatccccaaa aataatactt aagtacagta atcaagtaaa
     6301 attactcaag tactttacac ctctgcatgt gagcaaaagg ccagcaaaag gccaggaacc
     6361 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6421 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6481 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6541 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6601 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6661 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6721 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6781 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6841 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6901 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6961 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacact cagtggaacg
     7021 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7081 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7141 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7201 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7261 gccccagtgc tgcaatgata ccgcgggacc cacgctcacc ggctccagat ttatcagcaa
     7321 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7381 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7441 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7501 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7561 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7621 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7681 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7741 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7801 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7861 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7921 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7981 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8041 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8101 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat
     8161 ctcccgatcc cctagggtcg actctcagta caatctgctc tgatgccgca tagttaagcc
     8221 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     8281 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     8341 ttgcgctgct tcgcgatgta cgggccagat atacgcgggt accggcatat ggttcttgac
     8401 agaggtgtaa aaagtactca aaaattttac tcaagtgaaa gtacaagtac ttagggaaaa
     8461 ttttactcaa ttaaaagtaa aagtatctgg ctagaatctt acttgagtaa aagtaaaaaa
     8521 gtactccatt aaaattgtac ttgagtatta acgcgttgac attgattatt gactagttat
     8581 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     8641 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     8701 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     8761 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     8821 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     8881 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     8941 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     9001 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     9061 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     9121 gaggtctata taagc
//
