LOCUS       sequence-400573-        8423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  hU6-BsmBI-gRNA-Puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   Schmidheini L, Mathis N, Marquart KF, Rothgangl T, Kissling L, Bock 
            D, Chanez C, Wang JP, Jinek M, Schwank G
  TITLE     Continuous directed evolution of a compact CjCas9 variant with broad
            PAM compatibility.
  JOURNAL   Nat Chem Biol. 2023 Sep 21. doi: 10.1038/s41589-023-01427-x.
  PUBMED    37735239
REFERENCE   2  (bases 1 to 8423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2023 Sep 21. doi: 10.1038/s41589-023-01427-x."
COMMENT     Sequence Label: LentiGuide_CjCas9-Puro
FEATURES             Location/Qualifiers
     source          1..8423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    227..348
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(264..283)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     318..337
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      388..523
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(543..562)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(544..562)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(572..589)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(572..588)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(581..603)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      730..1185
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(817..836)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1027..1048
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1211..1315
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1316..1384,1385..2176)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1534..1553)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2347..2935
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2836..2855
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3089..3106
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3223..3244
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3259..3264,3265..3282,3283..3289)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3297..3313
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3302..3324
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3321..3337
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3321..3337
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3356..3376
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3358..3376
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3404..3630
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3631..3811
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3858..3983
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4476..4709
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4894..4938
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5116..5364
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5116..5136
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5287..5306
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5488..5560
                     /label=Cj gRNA scaffold
                     /note="guide RNA scaffold for the Campylobacter jejuni 
                     CRISPR/Cas9 system (Kim et al., 2016)"
     misc_feature    5617..5734
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5873..7051
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6104..7042
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6999..7019
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7064..7663
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7064..7083)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7560..7580
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7679..8267
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7732..7752)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8150..8161)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             join(8339..8423,1..149)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
       61 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
      121 cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat
      181 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
      241 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
      301 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
      361 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
      421 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
      481 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
      541 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
      601 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      661 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      721 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
      781 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
      841 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
      901 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
      961 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     1021 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     1081 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     1141 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     1201 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     1261 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     1321 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     1381 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1441 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     1501 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     1561 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     1621 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     1681 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     1741 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     1801 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     1861 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1921 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1981 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     2041 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     2101 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     2161 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2221 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2281 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2341 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2401 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2461 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     2521 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2581 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2641 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2701 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2761 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2821 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2881 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2941 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3001 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3061 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     3121 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     3181 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     3241 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     3301 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     3361 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
     3421 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     3481 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     3541 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     3601 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
     3661 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     3721 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     3781 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     3841 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     3901 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     3961 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     4021 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     4081 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     4141 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     4201 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     4261 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     4321 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     4381 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     4441 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     4501 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     4561 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     4621 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     4681 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     4741 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     4801 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     4861 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     4921 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     4981 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     5041 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     5101 ccgaggggac ccagagaggg cctatttccc atgattcctt catatttgca tatacgatac
     5161 aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa
     5221 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
     5281 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
     5341 atcttgtgga aaggacgaaa caccggagac ggttgtaaat gagcacacaa aatacatttt
     5401 ggggtaaatt tagttcctgt tccattaact gcgctaaaaa taatttttaa atctttttta
     5461 gcttcttgct cttttttgta cgtctctgtt ctagtccctg aaaagggact agaataaaga
     5521 gtttgcggga ctctgcgggg ttacaatccc ctaaaaccgc tttttttaag cttggcgtaa
     5581 ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     5641 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     5701 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     5761 tccactttgg cgccggctcg agggggcccg ggtgcaaaga tggataaagt tttaaacaga
     5821 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
     5881 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     5941 cggcaattga tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     6001 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     6061 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
     6121 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
     6181 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
     6241 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
     6301 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
     6361 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     6421 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     6481 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     6541 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     6601 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     6661 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     6721 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     6781 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     6841 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     6901 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     6961 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     7021 aagttttttt cttccatttc aggtgtcgtg acgtacggcc accatgaccg agtacaagcc
     7081 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     7141 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     7201 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     7261 cgcggacgac ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     7321 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     7381 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     7441 caccgtcgga gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     7501 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     7561 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     7621 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaacgcgtt aagtcgacaa
     7681 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     7741 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     7801 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     7861 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     7921 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     7981 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     8041 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     8101 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     8161 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     8221 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga
     8281 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     8341 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     8401 tggttagacc agatctgagc ctg
//
