LOCUS       sequence-400590-       12219 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For DNA insertion test in protoplast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12219)
  AUTHORS   Sun C, Lei Y, Li B, Gao Q, Li Y, Cao W, Yang C, Li H, Wang Z, Li Y, 
            Wang Y, Liu J, Zhao KT, Gao C
  TITLE     Precise integration of large DNA sequences in plant genomes using 
            PrimeRoot editors.
  JOURNAL   Nat Biotechnol. 2023 Apr 24. doi: 10.1038/s41587-023-01769-w.
  PUBMED    37095350
REFERENCE   2  (bases 1 to 12219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Apr 24. doi: 10.1038/s41587-023-01769-w."
COMMENT     Sequence Label: PrimeRoot.v2C-Cre
FEATURES             Location/Qualifiers
     source          1..12219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2009..2029
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2030..4543,4544..4546,4547..6130)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             6043..6051
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6353..6373
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8036..8056
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8153..8173
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9212..9259
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     polyA_signal    9319..9495
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(10049..10065)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10049..10065)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10062..10084)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10073..10089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10097..10103,10104..10121,10122..10127))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10142..10163
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10280..10297)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10451..11039)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10531..10550)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11210..12001,12002..12070))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11833..11852
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12071..12175)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
      721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
      781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
      841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
      901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
      961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccccctctct
     1021 accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca
     1081 tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc
     1141 gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc
     1201 tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg
     1261 catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg
     1321 gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg
     1381 ttctagatcg gagtagaatt aattctgttt caaactacct ggtggattta ttaattttgg
     1441 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
     1501 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
     1561 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
     1621 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
     1681 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
     1741 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
     1801 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
     1861 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
     1921 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
     1981 gtttggtgtt acttctgcaa agcttatgcc taagaaaaag agaaaagtgg acaagaagta
     2041 ctcgatcggc ctcgatattg ggactaactc tgttggctgg gccgtgatca ccgacgagta
     2101 caaggtgccc tcaaagaagt tcaaggtcct gggcaacacc gatcggcatt ccatcaagaa
     2161 gaatctcatt ggcgctctcc tgttcgacag cggcgagacg gctgaggcta cgcggctcaa
     2221 gcgcaccgcc cgcaggcggt acacgcgcag gaagaatcgc atctgctacc tgcaggagat
     2281 tttctccaac gagatggcga aggttgacga ttctttcttc cacaggctgg aggagtcatt
     2341 cctcgtggag gaggataaga agcacgagcg gcatccaatc ttcggcaaca ttgtcgacga
     2401 ggttgcctac cacgagaagt accctacgat ctaccatctg cggaagaagc tcgtggactc
     2461 cacagataag gcggacctcc gcctgatcta cctcgctctg gcccacatga ttaagttcag
     2521 gggccatttc ctgatcgagg gggatctcaa cccggacaat agcgatgttg acaagctgtt
     2581 catccagctc gtgcagacgt acaaccagct cttcgaggag aaccccatta atgcgtcagg
     2641 cgtcgacgcg aaggctatcc tgtccgctag gctctcgaag tctcggcgcc tcgagaacct
     2701 gatcgcccag ctgccgggcg agaagaagaa cggcctgttc gggaatctca ttgcgctcag
     2761 cctggggctc acgcccaact tcaagtcgaa tttcgatctc gctgaggacg ccaagctgca
     2821 gctctccaag gacacatacg acgatgacct ggataacctc ctggcccaga tcggcgatca
     2881 gtacgcggac ctgttcctcg ctgccaagaa tctgtcggac gccatcctcc tgtctgatat
     2941 tctcagggtg aacaccgaga ttacgaaggc tccgctctca gcctccatga tcaagcgcta
     3001 cgacgagcac catcaggatc tgaccctcct gaaggcgctg gtcaggcagc agctccccga
     3061 gaagtacaag gagatcttct tcgatcagtc gaagaacggc tacgctgggt acattgacgg
     3121 cggggcctct caggaggagt tctacaagtt catcaagccg attctggaga agatggacgg
     3181 cacggaggag ctgctggtga agctcaatcg cgaggacctc ctgaggaagc agcggacatt
     3241 cgataacggc agcatcccac accagattca tctcggggag ctgcacgcta tcctgaggag
     3301 gcaggaggac ttctaccctt tcctcaagga taaccgcgag aagatcgaga agattctgac
     3361 tttcaggatc ccgtactacg tcggcccact cgctaggggc aactcccgct tcgcttggat
     3421 gacccgcaag tcagaggaga cgatcacgcc gtggaacttc gaggaggtgg tcgacaaggg
     3481 cgctagcgct cagtcgttca tcgagaggat gacgaatttc gacaagaacc tgccaaatga
     3541 gaaggtgctc cctaagcact cgctcctgta cgagtacttc acagtctaca acgagctgac
     3601 taaggtgaag tatgtgaccg agggcatgag gaagccggct ttcctgtctg gggagcagaa
     3661 gaaggccatc gtggacctcc tgttcaagac caaccggaag gtcacggtta agcagctcaa
     3721 ggaggactac ttcaagaaga ttgagtgctt cgattcggtc gagatctctg gcgttgagga
     3781 ccgcttcaac gcctccctgg ggacctacca cgatctcctg aagatcatta aggataagga
     3841 cttcctggac aacgaggaga atgaggatat cctcgaggac attgtgctga cactcactct
     3901 gttcgaggac cgggagatga tcgaggagcg cctgaagact tacgcccatc tcttcgatga
     3961 caaggtcatg aagcagctca agaggaggag gtacaccggc tgggggaggc tgagcaggaa
     4021 gctcatcaac ggcattcggg acaagcagtc cgggaagacg atcctcgact tcctgaagag
     4081 cgatggcttc gcgaaccgca atttcatgca gctgattcac gatgacagcc tcacattcaa
     4141 ggaggatatc cagaaggctc aggtgagcgg ccagggggac tcgctgcacg agcatatcgc
     4201 gaacctcgct ggctcgccag ctatcaagaa ggggattctg cagaccgtga aggttgtgga
     4261 cgagctggtg aaggtcatgg gcaggcacaa gcctgagaac atcgtcattg agatggcccg
     4321 ggagaatcag accacgcaga agggccagaa gaactcacgc gagaggatga agaggatcga
     4381 ggagggcatt aaggagctgg ggtcccagat cctcaaggag cacccggtgg agaacacgca
     4441 gctgcagaat gagaagctct acctgtacta cctccagaat ggccgcgata tgtatgtgga
     4501 ccaggagctg gatattaaca ggctcagcga ttacgacgtc gatgccatcg ttccacagtc
     4561 attcctgaag gatgactcca ttgacaacaa ggtcctcacc aggtcggaca agaaccgggg
     4621 caagtctgat aatgttcctt cagaggaggt cgttaagaag atgaagaact actggcgcca
     4681 gctcctgaat gccaagctga tcacgcagcg gaagttcgat aacctcacaa aggctgagag
     4741 gggcgggctc tctgagctgg acaaggcggg cttcatcaag aggcagctgg tcgagacacg
     4801 gcagatcact aagcacgttg cgcagattct cgactcacgg atgaacacta agtacgatga
     4861 gaatgacaag ctgatccgcg aggtgaaggt catcaccctg aagtcaaagc tcgtctccga
     4921 cttcaggaag gatttccagt tctacaaggt tcgggagatc aacaattacc accatgccca
     4981 tgacgcgtac ctgaacgcgg tggtcggcac agctctgatc aagaagtacc caaagctcga
     5041 gagcgagttc gtgtacgggg actacaaggt ttacgatgtg aggaagatga tcgccaagtc
     5101 ggagcaggag attggcaagg ctaccgccaa gtacttcttc tactctaaca ttatgaattt
     5161 cttcaagaca gagatcactc tggccaatgg cgagatccgg aagcgccccc tcatcgagac
     5221 gaacggcgag acgggggaga tcgtgtggga caagggcagg gatttcgcga ccgtcaggaa
     5281 ggttctctcc atgccacaag tgaatatcgt caagaagaca gaggtccaga ctggcgggtt
     5341 ctctaaggag tcaattctgc ctaagcggaa cagcgacaag ctcatcgccc gcaagaagga
     5401 ctgggatccg aagaagtacg gcgggttcga cagccccact gtggcctact cggtcctggt
     5461 tgtggcgaag gttgagaagg gcaagtccaa gaagctcaag agcgtgaagg agctgctggg
     5521 gatcacgatt atggagcgct ccagcttcga gaagaacccg atcgatttcc tggaggcgaa
     5581 gggctacaag gaggtgaaga aggacctgat cattaagctc cccaagtact cactcttcga
     5641 gctggagaac ggcaggaagc ggatgctggc ttccgctggc gagctgcaga aggggaacga
     5701 gctggctctg ccgtccaagt atgtgaactt cctctacctg gcctcccact acgagaagct
     5761 caagggcagc cccgaggaca acgagcagaa gcagctgttc gtcgagcagc acaagcatta
     5821 cctcgacgag atcattgagc agatttccga gttctccaag cgcgtgatcc tggccgacgc
     5881 gaatctggat aaggtcctct ccgcgtacaa caagcaccgc gacaagccaa tcagggagca
     5941 ggctgagaat atcattcatc tcttcaccct gacgaacctc ggcgcccctg ctgctttcaa
     6001 gtacttcgac acaactatcg atcgcaagag gtacacaagc actaaggagg tcctggacgc
     6061 gaccctcatc caccagtcga ttaccggcct ctacgagacg cgcatcgacc tgtctcagct
     6121 cgggggcgac gaattctccg ggagcgagac gccaggcacc tccgagtcgg ccaccccaga
     6181 atctgccaca gtggtgtccg gccaaaagca ggaccgccag ggcggagaac gcagaaggtc
     6241 ccagctcgat agggatcagt gtgcctactg caaggagaag ggccactggg ccaaagactg
     6301 cccgaaaaag ccgcgcggcc cacgcggccc aaggccacaa acatccctcc ttccaaagaa
     6361 gaagcggaag gtggagctca gcggaggatc ttccggagga tctagcggct ccgagacacc
     6421 aggaacatcc gaaagcgcta caccagaatc tagcggaggc tcttccggag gatctaggcc
     6481 taccctcaac atcgaggatg agtatcgcct ccacgaaacc tccaaagaac cggacgtgtc
     6541 cctcggcagc acatggctca gcgacttccc acaagcgtgg gccgaaaccg gcggcatggg
     6601 cctcgccgtc cgccaagccc cactcattat cccgctgaag gcgacctcca caccggtgtc
     6661 catcaagcag tacccgatga gccaagaggc gaggctcggg attaagccgc acattcagcg
     6721 cctcctcgat caaggcattc tcgtgccgtg ccaatccccg tggaatacac cactcctccc
     6781 ggtcaaaaag ccgggcacca acgactatcg cccggtccaa gatctccgcg aggtcaacaa
     6841 gcgcgtggaa gacatccacc cgaccgtccc gaacccgtat aatctgctct ccgggctccc
     6901 accatcccac cagtggtata cagtgctgga cctcaaagac gccttcttct gtctccgcct
     6961 ccacccaaca agccagccgc tcttcgcctt cgagtggcgc gacccggaga tgggcatctc
     7021 cggccaactg acatggacac gcctcccgca aggcttcaag aacagcccga cactcttcaa
     7081 cgaggcgctc catagggacc tcgcggattt tcgcatccag catccggacc tcatcctcct
     7141 ccagtatgtg gatgatctcc tcctcgccgc gacctccgag ctggattgtc aacaaggcac
     7201 acgcgcgctc ctccaaacac tcgggaacct cggctatcgc gcgtccgcga aaaaggccca
     7261 aatctgccag aagcaagtga agtacctcgg gtatctgctc aaggaaggcc aacgctggct
     7321 caccgaagcg cgcaaagaaa cagtgatggg gcaaccgaca ccgaaaacac cacgccagct
     7381 gcgcgagttt ctcggcaaag ccggcttctg tcgcctcttc atcccgggct ttgccgagat
     7441 ggccgcgcca ctctacccac tcaccaagcc gggcacactg tttaactggg ggccggatca
     7501 gcagaaagcc taccaagaga tcaaacaagc gctcctcacc gccccagcgc tcgggctccc
     7561 agatctcaca aagccgttcg agctgttcgt cgatgagaag caaggctacg cgaagggcgt
     7621 gctcacacag aagctcggcc cgtggaggag gccagtggcc tatctctcca aaaaactcga
     7681 tccagtggcc gccggctggc caccgtgtct gcgcatggtc gccgcgattg ccgtgctcac
     7741 aaaggatgcc ggcaaactca caatgggcca gccgctggtg atcctcgcgc cacatgccgt
     7801 ggaagccctc gtcaaacagc cgccggatag gtggctctcc aatgcgcgca tgacccatta
     7861 ccaagcgctc ctcctcgaca ccgatcgcgt ccagttcggc ccagtggtcg ccctcaatcc
     7921 ggcgacactg ctgccactcc cagaggaggg cctccaacac aactgtctgg atattctcgc
     7981 ggaagcgcat ggcacaaggc cagacctcac agatcaaccg ctcagcggcg gcagcccgaa
     8041 gaagaaaagg aaggtgtccg gcggatcttc tggaggatct agcggctccg agacaccagg
     8101 aacatccgaa tccgctacac cagagtcttc tggaggatct agcggaggat ctccaaagaa
     8161 gaaaagaaag gtttctggcg gctccagtaa tctcttgacc gttcatcaaa atttaccggc
     8221 gctcccggtg gacgccacaa gcgatgaggt gcggaagaac ctgatggata tgttccgtga
     8281 tcggcaggcg tttagcgaac atacatggaa gatgcttctt tccgtgtgtc gtagttgggc
     8341 agcgtggtgc aagctcaaca accggaagtg gttccctgct gagccggaag acgtcaggga
     8401 ctatttactc tacctccaag ctagggggtt agcagttaaa actatacagc agcatctcgg
     8461 tcaattgaac atgctgcacc ggcgcagcgg actgccgcgg cccagtgact ctaatgctgt
     8521 gtcgctcgtg atgaggcgca tcagaaagga gaatgtggat gctggtgaac gagccaagca
     8581 ggcgcttgcc ttcgagagga cagattttga tcaagttcgc agcctcatgg agaacagcga
     8641 tcgttgccaa gacatcagga atctagcctt cctgggtatc gcctacaata ctctgttgag
     8701 aattgcggag attgcgagga tccgtgtgaa ggacataagc cgaactgatg gtgggaggat
     8761 gttgattcac attggacgca ccaagacact cgtatcaacc gcaggagtcg agaaggctct
     8821 atcgcttggg gttactaagt tggtcgaacg atggatatca gtatccggcg tcgccgacga
     8881 tccaaacaat tacctgttct gccgcgtgcg caagaatggg gttgcggcgc cttcagctac
     8941 gtcccagctc tcgacgaggg cactggaggg catctttgag gctacgcaca ggctgatcta
     9001 tggcgcgaaa gatgattccg gccagagata cttggcatgg tctggacact ccgccagagt
     9061 cggcgccgcc cgcgacatgg cgcgggccgg cgtgtcaatt ccagaaataa tgcaggcagg
     9121 agggtggacc aatgtcaaca ttgtaatgaa ctatatcaga aacctcgact cggagacagg
     9181 agccatggtc aggcttctag aagacggtga caagcgcccc gcagcgacca agaaagctgg
     9241 ccaggcaaag aaaaaaaaat gagcggccgc ggtacgctga aatcaccagt ctctctctac
     9301 aaatctatct ctctctattt tctccataaa taatgtgtga gtagtttccc gataagggaa
     9361 attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     9421 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     9481 actaaaatcc agatctccta aagtccctat agatctttgt cgtgaatata aaccagacac
     9541 gagacgacta aacctggagc ccagacgccg ttcgaagcta gaagtaccgc ttaggcagga
     9601 ggccgttagg gaaaagatgc taaggcaggg ttggttacgt tgactccccc gtaggtttgg
     9661 tttaaatatg atgaagtgga cggaaggaag gaggaagaca aggaaggata aggttgcagg
     9721 ccctgtgcaa ggtaagaaga tggaaatttg atagaggtac gctactatac ttatactata
     9781 cgctaaggga atgcttgtat ttatacccta taccccctaa taacccctta tcaatttaag
     9841 aaataatccg cataagcccc cgcttaaaaa ttggtatcag agccatgaat aggtctatga
     9901 ccaaaactca agaggataaa acctcaccaa aatacgaaag agttcttaac tctaaagata
     9961 aaagatcttt caagatcaaa actagttccc tcacaccggt gacggggatc gcatgcgata
    10021 tctcgagatc tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
    10081 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    10141 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
    10201 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    10261 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    10321 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    10381 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    10441 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    10501 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    10561 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    10621 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    10681 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    10741 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    10801 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    10861 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
    10921 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    10981 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    11041 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    11101 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    11161 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    11221 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    11281 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    11341 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    11401 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    11461 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    11521 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    11581 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    11641 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    11701 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    11761 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    11821 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    11881 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    11941 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    12001 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    12061 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    12121 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    12181 ttccccgaaa agtgccacct gccagtgcca agctaattc
//
