LOCUS       sequence-400593-       12465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For DNA insertion test in protoplast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12465)
  AUTHORS   Sun C, Lei Y, Li B, Gao Q, Li Y, Cao W, Yang C, Li H, Wang Z, Li Y, 
            Wang Y, Liu J, Zhao KT, Gao C
  TITLE     Precise integration of large DNA sequences in plant genomes using 
            PrimeRoot editors.
  JOURNAL   Nat Biotechnol. 2023 Apr 24. doi: 10.1038/s41587-023-01769-w.
  PUBMED    37095350
REFERENCE   2  (bases 1 to 12465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Apr 24. doi: 10.1038/s41587-023-01769-w."
COMMENT     Sequence Label: PrimeRoot.v2C-FLP
FEATURES             Location/Qualifiers
     source          1..12465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2009..2029
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2030..4543,4544..4546,4547..6130)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             6043..6051
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6353..6373
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8036..8056
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8153..8173
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9458..9505
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     polyA_signal    9565..9741
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(10295..10311)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10295..10311)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10308..10330)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10319..10335
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10343..10349,10350..10367,10368..10373))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10388..10409
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10526..10543)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10697..11285)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10777..10796)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11456..12247,12248..12316))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12079..12098
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12317..12421)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
      721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
      781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
      841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
      901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
      961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccccctctct
     1021 accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca
     1081 tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc
     1141 gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc
     1201 tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg
     1261 catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg
     1321 gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg
     1381 ttctagatcg gagtagaatt aattctgttt caaactacct ggtggattta ttaattttgg
     1441 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
     1501 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
     1561 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
     1621 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
     1681 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
     1741 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
     1801 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
     1861 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
     1921 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
     1981 gtttggtgtt acttctgcaa agcttatgcc taagaaaaag agaaaagtgg acaagaagta
     2041 ctcgatcggc ctcgatattg ggactaactc tgttggctgg gccgtgatca ccgacgagta
     2101 caaggtgccc tcaaagaagt tcaaggtcct gggcaacacc gatcggcatt ccatcaagaa
     2161 gaatctcatt ggcgctctcc tgttcgacag cggcgagacg gctgaggcta cgcggctcaa
     2221 gcgcaccgcc cgcaggcggt acacgcgcag gaagaatcgc atctgctacc tgcaggagat
     2281 tttctccaac gagatggcga aggttgacga ttctttcttc cacaggctgg aggagtcatt
     2341 cctcgtggag gaggataaga agcacgagcg gcatccaatc ttcggcaaca ttgtcgacga
     2401 ggttgcctac cacgagaagt accctacgat ctaccatctg cggaagaagc tcgtggactc
     2461 cacagataag gcggacctcc gcctgatcta cctcgctctg gcccacatga ttaagttcag
     2521 gggccatttc ctgatcgagg gggatctcaa cccggacaat agcgatgttg acaagctgtt
     2581 catccagctc gtgcagacgt acaaccagct cttcgaggag aaccccatta atgcgtcagg
     2641 cgtcgacgcg aaggctatcc tgtccgctag gctctcgaag tctcggcgcc tcgagaacct
     2701 gatcgcccag ctgccgggcg agaagaagaa cggcctgttc gggaatctca ttgcgctcag
     2761 cctggggctc acgcccaact tcaagtcgaa tttcgatctc gctgaggacg ccaagctgca
     2821 gctctccaag gacacatacg acgatgacct ggataacctc ctggcccaga tcggcgatca
     2881 gtacgcggac ctgttcctcg ctgccaagaa tctgtcggac gccatcctcc tgtctgatat
     2941 tctcagggtg aacaccgaga ttacgaaggc tccgctctca gcctccatga tcaagcgcta
     3001 cgacgagcac catcaggatc tgaccctcct gaaggcgctg gtcaggcagc agctccccga
     3061 gaagtacaag gagatcttct tcgatcagtc gaagaacggc tacgctgggt acattgacgg
     3121 cggggcctct caggaggagt tctacaagtt catcaagccg attctggaga agatggacgg
     3181 cacggaggag ctgctggtga agctcaatcg cgaggacctc ctgaggaagc agcggacatt
     3241 cgataacggc agcatcccac accagattca tctcggggag ctgcacgcta tcctgaggag
     3301 gcaggaggac ttctaccctt tcctcaagga taaccgcgag aagatcgaga agattctgac
     3361 tttcaggatc ccgtactacg tcggcccact cgctaggggc aactcccgct tcgcttggat
     3421 gacccgcaag tcagaggaga cgatcacgcc gtggaacttc gaggaggtgg tcgacaaggg
     3481 cgctagcgct cagtcgttca tcgagaggat gacgaatttc gacaagaacc tgccaaatga
     3541 gaaggtgctc cctaagcact cgctcctgta cgagtacttc acagtctaca acgagctgac
     3601 taaggtgaag tatgtgaccg agggcatgag gaagccggct ttcctgtctg gggagcagaa
     3661 gaaggccatc gtggacctcc tgttcaagac caaccggaag gtcacggtta agcagctcaa
     3721 ggaggactac ttcaagaaga ttgagtgctt cgattcggtc gagatctctg gcgttgagga
     3781 ccgcttcaac gcctccctgg ggacctacca cgatctcctg aagatcatta aggataagga
     3841 cttcctggac aacgaggaga atgaggatat cctcgaggac attgtgctga cactcactct
     3901 gttcgaggac cgggagatga tcgaggagcg cctgaagact tacgcccatc tcttcgatga
     3961 caaggtcatg aagcagctca agaggaggag gtacaccggc tgggggaggc tgagcaggaa
     4021 gctcatcaac ggcattcggg acaagcagtc cgggaagacg atcctcgact tcctgaagag
     4081 cgatggcttc gcgaaccgca atttcatgca gctgattcac gatgacagcc tcacattcaa
     4141 ggaggatatc cagaaggctc aggtgagcgg ccagggggac tcgctgcacg agcatatcgc
     4201 gaacctcgct ggctcgccag ctatcaagaa ggggattctg cagaccgtga aggttgtgga
     4261 cgagctggtg aaggtcatgg gcaggcacaa gcctgagaac atcgtcattg agatggcccg
     4321 ggagaatcag accacgcaga agggccagaa gaactcacgc gagaggatga agaggatcga
     4381 ggagggcatt aaggagctgg ggtcccagat cctcaaggag cacccggtgg agaacacgca
     4441 gctgcagaat gagaagctct acctgtacta cctccagaat ggccgcgata tgtatgtgga
     4501 ccaggagctg gatattaaca ggctcagcga ttacgacgtc gatgccatcg ttccacagtc
     4561 attcctgaag gatgactcca ttgacaacaa ggtcctcacc aggtcggaca agaaccgggg
     4621 caagtctgat aatgttcctt cagaggaggt cgttaagaag atgaagaact actggcgcca
     4681 gctcctgaat gccaagctga tcacgcagcg gaagttcgat aacctcacaa aggctgagag
     4741 gggcgggctc tctgagctgg acaaggcggg cttcatcaag aggcagctgg tcgagacacg
     4801 gcagatcact aagcacgttg cgcagattct cgactcacgg atgaacacta agtacgatga
     4861 gaatgacaag ctgatccgcg aggtgaaggt catcaccctg aagtcaaagc tcgtctccga
     4921 cttcaggaag gatttccagt tctacaaggt tcgggagatc aacaattacc accatgccca
     4981 tgacgcgtac ctgaacgcgg tggtcggcac agctctgatc aagaagtacc caaagctcga
     5041 gagcgagttc gtgtacgggg actacaaggt ttacgatgtg aggaagatga tcgccaagtc
     5101 ggagcaggag attggcaagg ctaccgccaa gtacttcttc tactctaaca ttatgaattt
     5161 cttcaagaca gagatcactc tggccaatgg cgagatccgg aagcgccccc tcatcgagac
     5221 gaacggcgag acgggggaga tcgtgtggga caagggcagg gatttcgcga ccgtcaggaa
     5281 ggttctctcc atgccacaag tgaatatcgt caagaagaca gaggtccaga ctggcgggtt
     5341 ctctaaggag tcaattctgc ctaagcggaa cagcgacaag ctcatcgccc gcaagaagga
     5401 ctgggatccg aagaagtacg gcgggttcga cagccccact gtggcctact cggtcctggt
     5461 tgtggcgaag gttgagaagg gcaagtccaa gaagctcaag agcgtgaagg agctgctggg
     5521 gatcacgatt atggagcgct ccagcttcga gaagaacccg atcgatttcc tggaggcgaa
     5581 gggctacaag gaggtgaaga aggacctgat cattaagctc cccaagtact cactcttcga
     5641 gctggagaac ggcaggaagc ggatgctggc ttccgctggc gagctgcaga aggggaacga
     5701 gctggctctg ccgtccaagt atgtgaactt cctctacctg gcctcccact acgagaagct
     5761 caagggcagc cccgaggaca acgagcagaa gcagctgttc gtcgagcagc acaagcatta
     5821 cctcgacgag atcattgagc agatttccga gttctccaag cgcgtgatcc tggccgacgc
     5881 gaatctggat aaggtcctct ccgcgtacaa caagcaccgc gacaagccaa tcagggagca
     5941 ggctgagaat atcattcatc tcttcaccct gacgaacctc ggcgcccctg ctgctttcaa
     6001 gtacttcgac acaactatcg atcgcaagag gtacacaagc actaaggagg tcctggacgc
     6061 gaccctcatc caccagtcga ttaccggcct ctacgagacg cgcatcgacc tgtctcagct
     6121 cgggggcgac gaattctccg ggagcgagac gccaggcacc tccgagtcgg ccaccccaga
     6181 atctgccaca gtggtgtccg gccaaaagca ggaccgccag ggcggagaac gcagaaggtc
     6241 ccagctcgat agggatcagt gtgcctactg caaggagaag ggccactggg ccaaagactg
     6301 cccgaaaaag ccgcgcggcc cacgcggccc aaggccacaa acatccctcc ttccaaagaa
     6361 gaagcggaag gtggagctca gcggaggatc ttccggagga tctagcggct ccgagacacc
     6421 aggaacatcc gaaagcgcta caccagaatc tagcggaggc tcttccggag gatctaggcc
     6481 taccctcaac atcgaggatg agtatcgcct ccacgaaacc tccaaagaac cggacgtgtc
     6541 cctcggcagc acatggctca gcgacttccc acaagcgtgg gccgaaaccg gcggcatggg
     6601 cctcgccgtc cgccaagccc cactcattat cccgctgaag gcgacctcca caccggtgtc
     6661 catcaagcag tacccgatga gccaagaggc gaggctcggg attaagccgc acattcagcg
     6721 cctcctcgat caaggcattc tcgtgccgtg ccaatccccg tggaatacac cactcctccc
     6781 ggtcaaaaag ccgggcacca acgactatcg cccggtccaa gatctccgcg aggtcaacaa
     6841 gcgcgtggaa gacatccacc cgaccgtccc gaacccgtat aatctgctct ccgggctccc
     6901 accatcccac cagtggtata cagtgctgga cctcaaagac gccttcttct gtctccgcct
     6961 ccacccaaca agccagccgc tcttcgcctt cgagtggcgc gacccggaga tgggcatctc
     7021 cggccaactg acatggacac gcctcccgca aggcttcaag aacagcccga cactcttcaa
     7081 cgaggcgctc catagggacc tcgcggattt tcgcatccag catccggacc tcatcctcct
     7141 ccagtatgtg gatgatctcc tcctcgccgc gacctccgag ctggattgtc aacaaggcac
     7201 acgcgcgctc ctccaaacac tcgggaacct cggctatcgc gcgtccgcga aaaaggccca
     7261 aatctgccag aagcaagtga agtacctcgg gtatctgctc aaggaaggcc aacgctggct
     7321 caccgaagcg cgcaaagaaa cagtgatggg gcaaccgaca ccgaaaacac cacgccagct
     7381 gcgcgagttt ctcggcaaag ccggcttctg tcgcctcttc atcccgggct ttgccgagat
     7441 ggccgcgcca ctctacccac tcaccaagcc gggcacactg tttaactggg ggccggatca
     7501 gcagaaagcc taccaagaga tcaaacaagc gctcctcacc gccccagcgc tcgggctccc
     7561 agatctcaca aagccgttcg agctgttcgt cgatgagaag caaggctacg cgaagggcgt
     7621 gctcacacag aagctcggcc cgtggaggag gccagtggcc tatctctcca aaaaactcga
     7681 tccagtggcc gccggctggc caccgtgtct gcgcatggtc gccgcgattg ccgtgctcac
     7741 aaaggatgcc ggcaaactca caatgggcca gccgctggtg atcctcgcgc cacatgccgt
     7801 ggaagccctc gtcaaacagc cgccggatag gtggctctcc aatgcgcgca tgacccatta
     7861 ccaagcgctc ctcctcgaca ccgatcgcgt ccagttcggc ccagtggtcg ccctcaatcc
     7921 ggcgacactg ctgccactcc cagaggaggg cctccaacac aactgtctgg atattctcgc
     7981 ggaagcgcat ggcacaaggc cagacctcac agatcaaccg ctcagcggcg gcagcccgaa
     8041 gaagaaaagg aaggtgtccg gcggatcttc tggaggatct agcggctccg agacaccagg
     8101 aacatccgaa tccgctacac cagagtcttc tggaggatct agcggaggat ctccaaaaaa
     8161 gaagaggaaa gtctccgggg gcagcagcat gccgcagttc gacatcctct gcaagacccc
     8221 gccgaaggtg ctcgtgaggc agttcgtgga gaggttcgag aggccgagcg gcgagaagat
     8281 cgccctctgc gccgccgagc tcacctacct ctgctggatg atcacccaca acggcaccgc
     8341 catcaagagg gccaccttca tgagctacaa caccatcatc agcaacagcc tcagcttcga
     8401 catcgtgaac aagagcctgc agttcaagta caagacacag aaggccacca tcctcgaggc
     8461 gagcctcaag aagctcatcc cggcctggga gttcaccatc atcccgtact acgggcagaa
     8521 gcatcagagc gacatcaccg acatcgtgag cagcctgcag ctgcagttcg agagcagcga
     8581 ggaggccgac aagggcaaca gccacagcaa gaagatgctc aaggccctcc tcagcgaggg
     8641 cgagagcatc tgggagatca ccgagaagat cctcaacagc ttcgagtaca caagccgctt
     8701 caccaagacc aagaccctct atcagttcct cttcctcgcc accttcatca actgcgggag
     8761 gttcagcgac atcaagaacg tggacccgaa gagcttcaag ctcgtgcaga acaagtacct
     8821 cggcgtgatc attcagtgcc tcgtgaccga aaccaagaca agcgtgagcc gccacatcta
     8881 cttcttcagc gcgaggggga ggatcgaccc gctcgtgtac ctcgacgagt tcctgaggaa
     8941 cagcgagccg gtgctcaaga gggtgaatag gaccggcaac agcagcagca acaagcaaga
     9001 gtatcagctc ctcaaggaca acctcgtgag gagctacaac aaggccctca agaagaacgc
     9061 cccgtacagc atcttcgcca tcaagaacgg cccgaagagc cacatcggga ggcacctcat
     9121 gacatccttc ctcagcatga agggcctcac cgagctcacc aacgtggtgg gcaactggag
     9181 cgacaagagg gcgagcgccg tggcgaggac cacctacacc catcagatca ccgccatccc
     9241 ggaccactac ttcgccctcg tgagccgcta ctacgcctac gacccgatca gcaaggagat
     9301 gatcgccctc aaggacgaaa ccaacccgat cgaggagtgg cagcacatcg agcagctcaa
     9361 gggcagcgcc gagggcagca ttaggtaccc ggcctggaac ggcatcatca gccaagaggt
     9421 gctcgactac ctcagcagct acatcaatag gaggatcaag cgccccgcag cgaccaagaa
     9481 agctggccag gcaaagaaaa aaaaatgagc ggccgcggta cgctgaaatc accagtctct
     9541 ctctacaaat ctatctctct ctattttctc cataaataat gtgtgagtag tttcccgata
     9601 agggaaatta gggttcttat agggtttcgc tcatgtgttg agcatataag aaacccttag
     9661 tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa
     9721 tccagtacta aaatccagat ctcctaaagt ccctatagat ctttgtcgtg aatataaacc
     9781 agacacgaga cgactaaacc tggagcccag acgccgttcg aagctagaag taccgcttag
     9841 gcaggaggcc gttagggaaa agatgctaag gcagggttgg ttacgttgac tcccccgtag
     9901 gtttggttta aatatgatga agtggacgga aggaaggagg aagacaagga aggataaggt
     9961 tgcaggccct gtgcaaggta agaagatgga aatttgatag aggtacgcta ctatacttat
    10021 actatacgct aagggaatgc ttgtatttat accctatacc ccctaataac cccttatcaa
    10081 tttaagaaat aatccgcata agcccccgct taaaaattgg tatcagagcc atgaataggt
    10141 ctatgaccaa aactcaagag gataaaacct caccaaaata cgaaagagtt cttaactcta
    10201 aagataaaag atctttcaag atcaaaacta gttccctcac accggtgacg gggatcgcat
    10261 gcgatatctc gagatctagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
    10321 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
    10381 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
    10441 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    10501 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    10561 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    10621 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    10681 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    10741 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    10801 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    10861 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    10921 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    10981 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    11041 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    11101 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    11161 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    11221 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    11281 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    11341 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    11401 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    11461 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    11521 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    11581 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    11641 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    11701 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    11761 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    11821 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    11881 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    11941 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    12001 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    12061 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    12121 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    12181 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    12241 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    12301 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    12361 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    12421 gcacatttcc ccgaaaagtg ccacctgcca gtgccaagct aattc
//
