LOCUS       sequence-400635-        3455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  the promoter can be recognized by PadR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Jiang T, Li C, Yan Y
  TITLE     Optimization of a p-Coumaric Acid Biosensor System for Versatile 
            Dynamic Performance.
  JOURNAL   ACS Synth Biol. 2021 Jan 15;10(1):132-144. doi: 
            10.1021/acssynbio.0c00500. Epub 2020 Dec 30.
  PUBMED    33378169
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00500"; journalName: "ACS Synth Biol"; date: 
            "2021-01-15- 15"; volume: "10"; issue: "1"; pages: "132-144"
COMMENT     Sequence Label: pZE-PpadC-yveF-yveG-egfp
FEATURES             Location/Qualifiers
     source          1..3455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             611..1327
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(641..669)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1264..1284
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1339..1425
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1590..2178)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1670..1689)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2266..2360)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2390..3181,3182..3250))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3013..3032
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3251..3355)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3423..3441
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagggac tgtcttcaaa cagtccttgt ttttttatgt tcctattgtt tgacagttaa
       61 ctgcaatggt gttaaagtga acatgtaaat agttacatga ttttttctga aggtgaggtg
      121 gttctcgtga agaagccggt tttaaaacca ttcgcctctt tagaaatcaa ggttgatccg
      181 cctatcacga ttggtgagac aagcctggga ctgagatgat tcattccgat tcgttcggga
      241 acaataaccg gggaagtaaa gggacgtatt ttgccgggcg gtgccgattc acaaatgatt
      301 cgcgctaacg gcagaacaga tttatctgcc aggtatgtga ttgaaacagc agatcatgaa
      361 ctgatttaca ttgaaaacaa tggaatacgg caagtcagca agccgtttcg aaaacaagcg
      421 gcagccgggg aaattattga accggagcat gtttattttc gtacggtacc gacgtttgaa
      481 acaggcagtg aagtctatca atggctccat gaccgcttgt ttatcggttc cgcagaaaga
      541 acccctgatt acgttctact agacatttat gaagtacagt aaaagactaa ggagagtgtg
      601 taagtgtacc atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
      661 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
      721 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
      781 gccaacactt gtcactactt tcgcgtatgg tcttcaatgc tttgcgagat acccagatca
      841 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
      901 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
      961 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
     1021 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
     1081 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
     1141 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     1201 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     1261 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     1321 caaatagtct agaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1381 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gccctagacc
     1441 tagggcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1501 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1561 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1621 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1681 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1741 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1801 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1861 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1921 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1981 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     2041 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2101 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2161 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2221 aacgaaaact cacgttaagg gattttggtc atgactagtg cttggattct caccaataaa
     2281 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     2341 tggatctatc aacaggagtc caagcgagct cgtaaacttg gtctgacagt taccaatgct
     2401 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2461 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2521 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2581 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2641 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2701 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2761 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     2821 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     2881 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2941 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3001 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3061 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3121 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3181 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3241 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3301 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3361 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     3421 aaaataggcg tatcacgagg ccctttcgtc ttcac
//
