LOCUS       sequence-400831-       12037 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SpRY Cas9 nickase cytosine base editor FNLS-BE3 and 
            blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12037)
  AUTHORS   Morris JA, Caragine C, Daniloski Z, Domingo J, Barry T, Lu L, Davis 
            K, Ziosi M, Glinos DA, Hao S, Mimitou EP, Smibert P, Roeder K, 
            Katsevich E, Lappalainen T, Sanjana NE
  TITLE     Discovery of target genes and pathways at GWAS loci by pooled 
            single-cell CRISPR screens.
  JOURNAL   Science. 2023 May 19;380(6646):eadh7699. doi: 
            10.1126/science.adh7699. Epub 2023 May 19.
  PUBMED    37141313
REFERENCE   2  (bases 1 to 12037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2023-05-19"; volume: "380(6646):eadh7699. doi"; pages: " 
            10.1126/science.adh7699. Epub 2023 May 19"
COMMENT     Sequence Label: lentiBE3-SpRY-Blast
FEATURES             Location/Qualifiers
     source          1..12037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..21
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3..21
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        49..275
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             276..456
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    503..628
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1121..1354
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1539..1583
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1767..1884
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1953..2164
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2184..2193
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2193..2258
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2265..2285
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2310..2996
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             3045..7145
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTRLGAPRAFKYFDTTIDPKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7176..7424
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7437..7457
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7467..7523
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7524..7922
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7938..8526
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7991..8011)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8409..8420)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8598..8831
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8909..9030
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8946..8965)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9000..9019
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9070..9205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9225..9244)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9226..9244)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9254..9271)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9254..9270)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9263..9285)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9412..9867
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9499..9518)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9709..9730
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9893..9997
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9998..10066,10067..10858)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10216..10235)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11029..11617
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11518..11537
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11771..11788
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11905..11926
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11941..11946,11947..11964,11965..11971)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11979..11995
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11984..12006
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12003..12019
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12003..12019
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
       61 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      121 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      181 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
      241 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
      301 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      361 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      421 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      481 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      541 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
      601 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
      661 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
      721 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
      781 ctgtagacaa atactgggac agctacaacc atcccttcag acaggattag aagaacttag
      841 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
      901 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
      961 gcaagcggcc gctgatcttc agacctggag gagaagatat gagggacaat tggagaagtg
     1021 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1081 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1141 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1201 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1261 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1321 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1381 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1441 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1501 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1561 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     1621 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     1681 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     1741 caaccccgag gggacccaga ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     1801 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     1861 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
     1921 cgccggctcg agtggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     1981 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     2041 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     2101 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     2161 acaggtgtcg tgacgcggga tccgccacca tggattacaa agatcatgac ggcgactata
     2221 aggaccacga tattgactac aaagatgatg acgacaagat ggctcccaaa aagaaaagga
     2281 aagtaggcat tcatggagtt cccgcagcca tgagttctga gactggtcca gtcgccgtcg
     2341 atccaaccct tcgacggagg attgagcccc acgagtttga agtatttttc gatccaagag
     2401 aattgcgaaa agagacatgt cttctgtacg agatcaactg ggggggccgc cacagtattt
     2461 ggagacacac cagccagaac acaaataagc atgtcgaggt aaatttcata gagaagttca
     2521 ctaccgaacg ctacttttgc cctaacacaa gatgtagcat tacttggttc ttgagttggt
     2581 ccccttgcgg tgaatgctca agagcaatta ccgagtttct gtctcggtat ccccacgtca
     2641 ctttgttcat ctacatagcc cgactttacc atcacgcaga ccctcgaaat cgccagggtc
     2701 ttagagattt gatttcatcc ggtgtaacca tacaaattat gaccgaacaa gagagtgggt
     2761 attgttggcg gaatttcgtc aactacagtc catcaaatga ggcccattgg ccccgctacc
     2821 cccacctttg ggtgagattg tacgtattgg agttgtattg cataatcctg ggccttcccc
     2881 cctgtcttaa catccttaga aggaagcagc cccagttgac attctttact atcgctcttc
     2941 agtcatgtca ctatcagcga ctgcctcctc atatcctttg ggctactggg ttgaaaagcg
     3001 gttccgagac acctggtact tcagagtccg ccacacccga aagtgacaag aagtacagca
     3061 tcggcctggc catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg
     3121 tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc
     3181 tgatcggagc cctgctgttc gacagcggcg aaacagccga gagaacccgg ctgaagagaa
     3241 ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca
     3301 gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg
     3361 tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg
     3421 cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg
     3481 acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc
     3541 acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc
     3601 agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg
     3661 acgccaaggc catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg
     3721 cccagctgcc cggcgagaag aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg
     3781 gcctgacccc caacttcaag agcaacttcg acctggccga ggatgccaaa ctgcagctga
     3841 gcaaggacac ctacgacgac gacctggaca acctgctggc ccagatcggc gaccagtacg
     3901 ccgacctgtt tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga
     3961 gagtgaacac cgagatcacc aaggcccccc tgagcgcctc tatgatcaag agatacgacg
     4021 agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt
     4081 acaaagagat tttcttcgac cagagcaaga acggctacgc cggctacatt gacggcggag
     4141 ccagccagga agagttctac aagttcatca agcccatcct ggaaaagatg gacggcaccg
     4201 aggaactgct cgtgaagctg aacagagagg acctgctgcg gaagcagcgg accttcgaca
     4261 acggcagcat cccccaccag atccacctgg gagagctgca cgccattctg cggcggcagg
     4321 aagattttta cccattcctg aaggacaacc gggaaaagat cgagaagatc ctgaccttcc
     4381 gcatccccta ctacgtgggc cctctggcca ggggaaacag cagattcgcc tggatgacca
     4441 gaaagagcga ggaaaccatc accccctgga acttcgagga agtggtggac aagggcgctt
     4501 ccgcccagag cttcatcgag cggatgacca acttcgataa gaacctgccc aacgagaagg
     4561 tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt gtataacgag ctgaccaaag
     4621 tgaaatacgt gaccgaggga atgagaaagc ccgccttcct gagcggcgag cagaaaaagg
     4681 ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg
     4741 actacttcaa gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt
     4801 tcaacgcctc cctgggcaca taccacgatc tgctgaaaat tatcaaggac aaggacttcc
     4861 tggacaatga ggaaaacgag gacattctgg aagatatcgt gctgaccctg acactgtttg
     4921 aggacagaga gatgatcgag gaacggctga aaacctatgc ccacctgttc gacgacaaag
     4981 tgatgaagca gctgaagcgg cggagataca ccggctgggg caggctgagc cggaagctga
     5041 tcaacggcat ccgggacaag cagtccggca agacaatcct ggatttcctg aagtccgacg
     5101 gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg
     5161 acatccagaa agcccaggtg tccggccagg gcgatagcct gcacgagcac attgccaatc
     5221 tggccggcag ccccgccatt aagaagggca tcctgcagac agtgaaggtg gtggacgagc
     5281 tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt gatcgaaatg gccagagaga
     5341 accagaccac ccagaaggga cagaagaaca gccgcgagag aatgaagcgg atcgaagagg
     5401 gcatcaaaga gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc
     5461 agaacgagaa gctgtacctg tactacctgc agaatgggcg ggatatgtac gtggaccagg
     5521 aactggacat caaccggctg tccgactacg atgtggacca tatcgtgcct cagagctttc
     5581 tgaaggacga ctccatcgac aacaaggtgc tgaccagaag cgacaagaac cggggcaaga
     5641 gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa gaactactgg cggcagctgc
     5701 tgaacgccaa gctgattacc cagagaaagt tcgacaatct gaccaaggcc gagagaggcg
     5761 gcctgagcga actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga
     5821 tcacaaagca cgtggcacag atcctggact cccggatgaa cactaagtac gacgagaatg
     5881 acaagctgat ccgggaagtg aaagtgatca ccctgaagtc caagctggtg tccgatttcc
     5941 ggaaggattt ccagttttac aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg
     6001 cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg
     6061 agttcgtgta cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc
     6121 aggaaatcgg caaggctacc gccaagtact tcttctacag caacatcatg aactttttca
     6181 agaccgagat taccctggcc aacggcgaga tccggaagcg gcctctgatc gagacaaacg
     6241 gcgaaaccgg ggagatcgtg tgggataagg gccgggattt tgccaccgtg cggaaagtgc
     6301 tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca
     6361 aagagtctat cagacccaag aggaacagcg ataagctgat cgccagaaag aaggactggg
     6421 accctaagaa gtacggcggc ttcctgtggc ccaccgtggc ctattctgtg ctggtggtgg
     6481 ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca
     6541 ccatcatgga aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct
     6601 acaaagaagt gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg
     6661 aaaacggccg gaagagaatg ctggcctctg ccaagcagct gcagaaggga aacgaactgg
     6721 ccctgccctc caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg
     6781 gctcccccga ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg
     6841 acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc
     6901 tggacaaagt gctgtccgcc tacaacaagc accgggataa gcccatcaga gagcaggccg
     6961 agaatatcat ccacctgttt accctgacca gactgggagc ccctagagcc ttcaagtact
     7021 ttgacaccac catcgacccc aagcagtaca gaagcaccaa agaggtgctg gacgccaccc
     7081 tgatccacca gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag
     7141 gtgacagcgg cgggagcggc gggagcggcg ggagcactaa tctgtcagat attattgaaa
     7201 aggagaccgg taagcaactg gttatccagg aatccatcct catgctccca gaggaggtgg
     7261 aagaagtcat tgggaacaag ccggaaagcg atatactcgt gcacaccgcc tacgacgaga
     7321 gcaccgacga gaatgtcatg cttctgacta gcgacgcccc tgaatacaag ccttgggctc
     7381 tggtcataca ggatagcaac ggtgagaaca agattaagat gctctctggt ggttctccca
     7441 agaagaagag gaaagtcgga tccggcgcaa caaacttctc tctgctgaaa caagccggag
     7501 atgtcgaaga gaatcctgga ccgatggcca agcctttgtc tcaagaagaa tccaccctca
     7561 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     7621 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     7681 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     7741 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     7801 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     7861 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
     7921 aaacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     7981 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     8041 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     8101 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     8161 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     8221 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     8281 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     8341 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     8401 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     8461 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     8521 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     8581 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     8641 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8701 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     8761 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8821 aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc
     8881 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     8941 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     9001 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     9061 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     9121 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     9181 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     9241 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     9301 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     9361 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     9421 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     9481 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     9541 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     9601 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     9661 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     9721 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     9781 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     9841 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     9901 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     9961 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
    10021 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
    10081 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
    10141 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
    10201 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
    10261 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
    10321 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
    10381 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
    10441 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
    10501 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
    10561 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
    10621 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
    10681 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
    10741 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
    10801 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
    10861 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    10921 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    10981 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    11041 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    11101 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    11161 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    11221 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    11281 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    11341 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    11401 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
    11461 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    11521 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    11581 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    11641 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
    11701 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
    11761 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
    11821 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
    11881 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
    11941 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
    12001 cacaggaaac agctatgacc atgattacgc caagcgc
//
